Tissue engineering bone tissue and preparation method thereof

A tissue engineering bone and tissue engineering technology, applied in the direction of bone implants, etc., can solve the problems of lack of basic mechanical stiffness, lack of bone mineral, lack of basic mechanical strength, etc., to achieve good biodegradability and physical and mechanical properties, The effect of good 3D connectivity and good biocompatibility

Inactive Publication Date: 2013-06-19
XIAN REJE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The former does not contain organic components, so it is loose and brittle, and does not have basic mechanical strength; the latter lacks bone mineral, so its texture is soft and does not have basic mechanical rigidity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) In vitro isolation and culture of bone marrow mesenchymal stem cells:

[0032] 10ml of the patient's autologous bone marrow was extracted, separated, and nucleated cells were extracted, washed twice with PBS+EDTA. Place in fibronectin-coated culture dishes, add DMEM culture medium, and incubator at 37°C containing 5% carbon dioxide. Two days later, unattached cells were removed and cultured in DMEM medium.

[0033] Subculture: When the cells are confluent to 60%-75% density, rinse with calcium and magnesium-free PBS, digest with trypsin, add DMEM medium containing fetal bovine serum to stop digestion, transfer to a centrifuge tube, and centrifuge. Discard the supernatant, add DMEM culture medium containing DMEM bovine serum, mix well, count, and subculture at a ratio of 1:2. Change the culture medium every 2-3 days, and subculture again when the cells reach a density of 60%-75%.

[0034] 2) Bone marrow mesenchymal stem cells are inoculated on cancellous bone scaff...

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Abstract

The invention discloses tissue engineering bone tissue and a preparation method thereof. The tissue engineering bone tissue is characterized in that the tissue engineering bone tissue is composed of a bone cortex frame constructed by reshaped cortical bone support materials and sponge bones filled in hollow positions of the bone cortex frame, and the reshaped cortical bone support materials are molded according to specific coloboma shapes of bones which need repairing. The tissue engineering bone tissue has a cortical bone, a sponge bone and cellular constituents similar to those of normal bone tissue. In clinical application, a physician can pre-determine the resection range according to a computed tomography (CT) slice of a patient, and reshapes the tissue engineering bone support materials according to CT reconstruction data of the coloboma part so as to achieve individual treatment.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a tissue engineering bone tissue and a preparation method thereof. Background technique [0002] Tissue engineering is a hot spot in life science in the 21st century. Its core content is three-dimensional cell culture in vitro, and its basic method is to form a complex of seed cells and biodegradable biomaterials to replace damaged tissues and organs. At present, autologous bone is mainly used as bone tissue substitutes at home and abroad, accounting for about 70%. Relatively successful in xenograft bone are some bone mineral materials that completely remove the organic components of bone, such as KEIL bone, BIOOSS bone, inorganic bone, and organic component materials that completely remove bone mineral components, such as demineralized bone, bone matrix gelatin, etc. The former does not contain organic components, so it is loose and brittle, and does not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/28
Inventor 孙叶芳
Owner XIAN REJE BIOLOGICAL TECH
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