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Application of ARRDC2 in evaluating development process of oral squamous cell carcinoma

A technology for ARRDC2 and oral squamous cell carcinoma, applied in the field of biomedicine, can solve the problems of lack of quantitative indicators, affecting the objectivity, accuracy and repeatability of tumor grading, achieve individualized treatment, avoid insufficient treatment intensity, and avoid overtreatment Effect

Active Publication Date: 2019-03-08
XIANGYA STOMATOLOGICAL HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complexity and heterogeneity of tumor tissue structure, different types of tumors (such as adenocarcinoma, squamous cell carcinoma, renal cell carcinoma, breast cancer, etc.) have different structural characteristics and grading standards, and there is a lack of quantitative indicators In addition, due to the influence of the sufficient degree of sampling and the subjective differences in diagnostic criteria and interpretation of atypia, the objectivity, accuracy and repeatability of tumor grading are affected to varying degrees.

Method used

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  • Application of ARRDC2 in evaluating development process of oral squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Screening Gene Markers Related to Oral Squamous Cell Carcinoma

[0043] 1. Sample collection

[0044] 68 oral squamous cell carcinoma tissues and adjacent tissues were collected, including 20 patients with histological grade I (G1), 30 patients with histological grade II (G2), and histological grade III (G3 / G4) patients in 18 cases. The patients did not receive any treatment before operation. All the above-mentioned samples were obtained with the consent of the organizational ethics committee, and 4 samples were taken from each group for detection and analysis of gene expression profiles, screening of differentially expressed genes, and verification experiments were carried out in all samples of each group.

[0045] 2. Preparation of RNA samples (operated using QIAGEN tissue RNA extraction kit)

[0046] Take out the tissue samples frozen in liquid nitrogen, put the tissue samples into a pre-cooled mortar for grinding, and extract and isolate RNA according t...

Embodiment 2

[0061] Example 2 QPCR sequencing to verify the differential expression of the ARRDC2 gene

[0062] 1. Large-sample QPCR verification of differential expression of ARRDC2 gene.

[0063] 2. The RNA extraction steps are as described in Example 1.

[0064] 3. Reverse transcription:

[0065] Use FastQμant cDNA First Strand Synthesis Kit (Product No.: KR106) for reverse transcription of mRNA, first remove the genomic DNA reaction, add 5×gDNA Bμffer 2.0μl, total RNA 1μg, add RNase Free ddH 2 O Make the total volume to 10μl, heat in a water bath at 42°C for 3min, then add 2.0μl of 10×Fast RT Bμffer, 1.0μl of RT Enzyme Mix, 2.0μl of FQ-RT Primer Mix, RNase Free ddH 2 O5.0 μl, after mixing, add to the above-mentioned test tube and mix together a total of 20 μl, heat at 42°C for 15 minutes in a water bath, and heat at 95°C for 3 minutes.

[0066] 4. QPCR amplification detection

[0067] QPCR amplification primers were designed according to the sequences of ARRDC2 and GAPDH, and synth...

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Abstract

The invention discloses an application of ARRDC2 in evaluating the development process of oral squamous cell carcinoma. According to the invention, by means of high throughput sequencing and bioinformatics analysis, gene ARRDC2, which indicates significant changes in different development processes of the tumor, is screened, and ARRDC2 is suggested, as a marker molecule, to be applied to determination of the development of the oral squamous cell carcinoma, prediction of the prognosis of the tumor and guiding of therapeutic plans and curative effect monitoring.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of ARRDC2 in evaluating the development process of oral squamous cell carcinoma. Background technique [0002] Oral squamous cell carcinoma (OSCC) is the most common malignant tumor of the oral cavity. There are 500,000 new cases worldwide each year, which poses a huge challenge and burden to the medical security system of the whole society. The susceptibility of genetic background and environmental factors lead to the occurrence and development of oral squamous cell carcinoma. Due to frequent distant metastasis, poor prognosis and high recurrence rate, the survival time of oral squamous cell carcinoma patients is shorter. At present, most patients with oral squamous cell carcinoma are already in the advanced stage of the disease when they are diagnosed. Therefore, an appropriate tumor-related marker will help the early diagnosis and prognosis of oral squamous cell carcin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886G01N33/574
CPCG01N33/57407G01N33/57484C12Q1/6886C12Q2600/158
Inventor 唐瞻贵王月红潘灏邓智元刘欧胜方小丹顾立群全宏志王柏胜戴小寒李龙陈晓婧
Owner XIANGYA STOMATOLOGICAL HOSPITAL CENT SOUTH UNIV
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