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Target gene for diagnosis of oral squamous cell carcinoma and application thereof

An oral squamous cell carcinoma and chain reaction technology, applied in the field of biomedicine, can solve the problems affecting the quality of life of patients and poor prognosis, and achieve the effect of avoiding insufficient treatment intensity, individualized treatment, and avoiding excessive treatment.

Inactive Publication Date: 2019-06-14
THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its special anatomical location, oral squamous cell carcinoma often seriously affects the quality of life of patients and most of them have a poor prognosis

Method used

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  • Target gene for diagnosis of oral squamous cell carcinoma and application thereof
  • Target gene for diagnosis of oral squamous cell carcinoma and application thereof
  • Target gene for diagnosis of oral squamous cell carcinoma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Screening Gene Markers Related to Oral Squamous Cell Carcinoma

[0047] 1. Sample collection

[0048] 59 oral squamous cell carcinoma tissues and adjacent tissues were collected, including 15 patients with histological grade I (G1), 23 patients with histological grade II (G2), and histological grade III There were 21 patients (G3 / G4) who did not receive any treatment before operation. Four samples were taken from each group for high-throughput sequencing analysis, differentially expressed genes were screened, and verification experiments were performed in all samples of each group.

[0049] 2. RNA sample preparation and quality testing

[0050] Utilize Tiangen's animal tissue total RNA extraction kit (catalogue number DP431) to extract total RNA, the steps are as follows:

[0051] 1) Homogenization treatment

[0052] Add 300μl Lysis Solution RL for every 10-20mg tissue, and grind the tissue thoroughly with a grinding pestle; then add 590μl RNase-Free ddH t...

Embodiment 2

[0074] Example 2 QPCR sequencing to verify the differential expression of IQCD genes

[0075] 1. Perform QPCR verification of all collected samples for differential expression of IQCD genes.

[0076] 2. The RNA extraction steps are as described in Example 1.

[0077] 3. QPCR

[0078] Primers were designed according to the gene sequences of IQCD and GADPH, wherein the common region between different transcripts of IQCD was selected for primer design, and the primer sequences are shown in Table 1.

[0079] Table 1 Primer Sequence

[0080]

[0081] The Quant one-step reverse transcription-fluorescence quantitative kit (SYBR Green) kit (catalogue number: NG105) from TIANGEN Company was used for PCR reaction, and the reaction system and reaction conditions are shown in Table 2. In Thermal Cycler Perform PCR amplification on the Real Time System amplification instrument, confirm the amplification curve and melting curve of Real Time PCR after the reaction, and perform relati...

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Abstract

The invention discloses a target gene for diagnosis of oral squamous cell carcinoma and application thereof. By screening genes with an expression level related to a tumor differentiation level, the expression of genes in tumor tissues in each differentiation level is determined, so that the differentiation level of a tumor is determined, thereby accurately and objectively predicting the tumor prognosis, guiding the formulation of a treatment plan and monitoring a therapeutic effect.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a target gene for diagnosis of oral squamous cell carcinoma and application thereof, and the specific gene involved is IQCD. Background technique [0002] Oral squamous cell carcinoma (OSCC) is a very destructive and fatal malignant tumor, which accounts for a large proportion of all head and neck malignant tumors and has become a major killer of human health. Because of its special anatomical location, oral squamous cell carcinoma often seriously affects the quality of life of patients and most of them have a poor prognosis. People's daily bad habits, such as smoking and drinking, are risk factors for cancer metastasis, and tumor metastasis is a serious problem faced by cancer patients. According to statistics, more than 90% of cancer-related mortality is due to metastasis. In oral squamous cell carcinoma, more than 50% of patients have lymph node metastasis, and the 5-year survival ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11G01N33/574
Inventor 项海东高琪程东梅
Owner THE SECOND HOSPITAL OF HEBEI MEDICAL UNIV
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