Marker used for assisted detection of abrupt highland advancing low pressure low oxygen environment related myocardial damages

Abstract

The invention discloses a marker used for assisted detection of abrupt highland advancing low pressure low oxygen environment related myocardial damages, and applications of a product used for detection of miR-144-3p expression level in preparation of kits used for auxiliary diagnosis of myocardial damages caused by low pressure low oxygen. According to the applications, a low-pressure oxygen cabin is adopted for stimulation of highland low pressure low oxygen environment, and establishment of rat animal models with low pressure low oxygen myocardial damages. High flux sequencing technology and bioinformatics methods are adopted, key miRNA related to highland low pressure low oxygen myocardial damages are screened, and fluorogenic quantitative PCR is adopted for experiment verifying of thekey miRNAs in cardiac muscle tissues and blood plasma. The miRNAs disclosed in the invention can be used for assisted detection of abrupt highland advancing low pressure low oxygen environment related myocardial damages, and possess important meaning in diagnosis and assessment of abrupt highland advancing low pressure low oxygen environment related myocardial damages.

Description

technical field [0001] The invention relates to a marker for assisting in the detection of myocardial injury associated with rapid plateau hypobaric hypoxic environment. Background technique [0002] MicroRNA (miRNA) is a kind of highly conserved non-coding RNA, which induces mRNA degradation or inhibits its translation into protein by binding to specific complementary sequences in target gene mRNA, and negatively regulates gene expression at the post-transcriptional level. miRNA plays a key role in many important life processes (such as cell growth, tissue differentiation, cell proliferation, embryonic development, cell apoptosis, etc.). There are about 2,000 known human miRNAs. Researchers have isolated and purified miRNA in various types of body fluids such as blood, urine, ascites, and pleural fluid. These miRNAs can undergo different rapid and highly sensitive changes in different pathophysiological states, suggesting that intercellular information exchange can be ach...

AI Technical Summary

Problems solved by technology

Due to the complex occurrence and development of heart disease caused by plateau hypobaric hypoxia, there are no clear diagnostic criteria and intervention strategies, and there is no simple and easy diagnosis and evaluation method for myocardial injury caused by plateau hypobaric hypoxia

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