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Intramuscular fat deposition related adiponectin gene

A technology of intramuscular fat and genetics, applied in genetic engineering, plant genetic improvement, animal/human peptides, etc., can solve the problem of little understanding of the molecular mechanism of intramuscular fat deposition in the growth and development of Banna miniature pigs

Inactive Publication Date: 2012-08-15
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
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  • Application Information

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Problems solved by technology

However, little is known about the growth and development and molecular mechanisms of intramuscular fat deposition in Banna minipigs

Method used

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  • Intramuscular fat deposition related adiponectin gene
  • Intramuscular fat deposition related adiponectin gene
  • Intramuscular fat deposition related adiponectin gene

Examples

Experimental program
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Embodiment Construction

[0018] A kind of intramuscular fat deposition Adiponectin gene, is the adiponectin (Adiponectin) gene related to intramuscular fat deposition isolated and cloned from Banna miniature pig muscle tissue, characterized in that: the full length of the CDS sequence of the gene is 732bp, its The CDS nucleotide sequence is shown in the description of the nucleotide and amino acid sequence listing SEQ.ID.NO.1. In addition, the CDS nucleotide sequence encodes 243 amino acids, and the amino acid sequence is as shown in SEQ.ID.NO.2 in the specification nucleotide and amino acid sequence table.

[0019] Primers were designed for the Adiponectin gene of the above-mentioned characteristics of intramuscular fat deposition, and PCR amplification was performed to detect the tissue expression profile of the Adiponectin gene in pig heart, liver, spleen, lung, kidney, muscle and fat. The expression level was the highest in tissues, preliminarily indicating that Adiponectin gene regulates the func...

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Abstract

The invention discloses an intramuscular fat deposition related adiponectin gene, which is obtained from Banna minipig muscular tissues by separating and cloning. The full length of a CDS (coding sequence) of the gene is 732dp, a nucleotide CDS of the gene is shown in SEQ. ID. NO.1, 243 amino acids are coded in the nucleotide CDS, and amino acid sequence is shown in SEQ. ID. NO.2. Results of detection of the adiponectin gene in pig heart, livers, spleen, lungs, kidneys, muscle and fat show that the adiponectin gene is highest in expression quantity in fat tissues, and the function of regulating fat deposition in fat cells is preliminarily indicated for the adiponectin gene.

Description

technical field [0001] The invention relates to the genetic engineering technology of animal molecular breeding, in particular to a gene technology of adiponectin related to pig intramuscular fat deposition. Background technique [0002] In recent years, due to the excessive pursuit of high lean meat percentage in pig breeding work, the quality of pork has seriously declined. With the continuous improvement of living standards, people have higher and higher requirements for meat quality characteristics such as pork color, tenderness, and flavor. Intramuscular fat (IMF) is an important factor affecting meat quality, and has been increasingly recognized and valued. The deposition of intramuscular fat is a complex physiological and biochemical process, which mainly involves the metabolism of intramuscular triglycerides, including the synthesis, decomposition and transport of triglycerides. Therefore, lipid metabolism in intramuscular adipocytes is the basis for intramuscular ...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/47C12Q1/68
Inventor 高士争潘洪彬黄英赵素梅王静王聪刘维全朱宝利
Owner YUNNAN AGRICULTURAL UNIVERSITY
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