FTO gene for intramuscular fat deposition

A technology for intramuscular fat and fat deposition, applied in genetic engineering, plant genetic improvement, animal/human peptides, etc.

Inactive Publication Date: 2012-07-18
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the effect of FTO gene expression on pig intramuscular fat deposition and lipid metabolism and its regulatory mechanism.

Method used

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  • FTO gene for intramuscular fat deposition
  • FTO gene for intramuscular fat deposition
  • FTO gene for intramuscular fat deposition

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Experimental program
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Embodiment Construction

[0021] A kind of intramuscular fat deposition FTO gene is a fat deposition and obesity associated (Fat mass and obesity associated, FTO) gene related to intramuscular fat deposition isolated and cloned from Banna mini pig muscle tissue, characterized in that: The full length of the CDS sequence is 1518bp, and its CDS nucleotide sequence is shown in SEQ.ID.NO.1. Moreover, the CDS nucleotide sequence encodes 505 amino acids, and the amino acid sequence is as SEQ.ID.NO.2.

[0022] Primers were designed for the FTO gene of intramuscular fat deposition with the above characteristics, and PCR amplification was performed to detect the tissue expression profile of FTO gene in seven tissues of pig heart, liver, spleen, lung, kidney, muscle and fat. A large amount of expression in the tissue preliminarily shows that the FTO gene regulates the function of fat deposition in adipocytes.

[0023] 1. Extraction of total RNA from muscle tissue

[0024] Take 50kg of adult Banna mini-pig ...

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Abstract

The invention discloses an FTO gene for intramuscular fat deposition. The FTO gene is an FTO (fat mass and obesity associated) gene which is separated and cloned from Banna miniature pig muscle tissue and is associated with intramuscular fat deposition. The whole length of the CDA (Complete Coding Sequence) of the gene is 1518bp and the CDS nucleotide sequence is shown as SEQ.ID.NO.1. In addition, the CDS nucleotide sequence codes 505 amino acids of which the amino acid sequence is shown as SEQ.ID.NO.2. Through the detection of a tissue expression profile of the FTO gene in seven tissues, such as pig heart, liver, spleen, lung, kidney, muscle and fat, the result shows that the FTO gene is greatly expressed in the fat tissue and the functions of the FTO gene for regulating and controlling the fat deposition in fat cells are preliminarily shown.

Description

technical field [0001] The invention relates to the genetic engineering technology of animal molecular breeding, in particular to a fat mass and obesity associated (FTO) gene technology related to intramuscular fat deposition in pigs. Background technique [0002] In recent years, due to the excessive pursuit of high lean meat percentage in pig breeding work, the quality of pork has seriously declined. With the continuous improvement of living standards, people have higher and higher requirements for meat quality characteristics such as pork color, tenderness, and flavor. Intramuscular fat (IMF) is an important factor affecting meat quality, and has been increasingly recognized and valued. The deposition of intramuscular fat is a complex physiological and biochemical process, which mainly involves the metabolism of intramuscular triglycerides, including the synthesis, decomposition and transport of triglycerides. Therefore, lipid metabolism in intramuscular adipocytes is t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/47C12Q1/68
Inventor 高士争黄英杨明华赵素梅王静潘洪彬
Owner YUNNAN AGRICULTURAL UNIVERSITY
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