Method for detecting sedative drug metabolism residues in animal tissues

Abstract

The invention discloses a method for detecting sedative drug metabolism residues in animal tissues, which adopts a dispersive solid-phase extraction-high performance liquid chromatography triple quadrupole mass spectrometry method to simultaneously determine chlorpromazine, promethazine, methylthiazine and metabolites thereof such as chlorpromazine sulfoxide, 2-chlorophenothiazine, promethazine sulfoxide, 2, 6-dimethylaniline in pork, pork liver, pig kidney, pig heart and pig lung. The method optimizes the detection conditions, can accurately detect the sedative drugs in animal tissues, has accurate and reliable detection results, provides reliable data for the measurement of sedative drug metabolic residues, has significant economic benefits, and has wide market prospects. The method has a good reference value for guiding the measurement of drug residues.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a detection method for metabolic residues of sedative drugs in animal tissues. Background technique [0002] Chlorpromazine, promethazine, xylazine and other phenothiazines have been found to have some special effects other than clinical sedation, such as improving meat quality (like "clenbuterol") and water retention. In order to alleviate the stress response of pigs during transportation and slaughter, some slaughterhouses use sedatives to pigs to eliminate restlessness. Driven by economic interests, some producers add such drugs during the breeding process to increase weight and fatten, shorten the time for slaughter, reduce losses caused by stress, and coordinate water injection. Such drugs and their metabolites will inevitably remain in pigs, and when such drug residues enter the human body through the food chain and reach a certain concentration, symptoms such as nausea,...

AI Technical Summary

Problems solved by technology

However, according to reports, during the general survey on the residues of sedative drugs and their metabolites in animal-derived foods, it was found that some pork may contain trace amounts of promethazine sulfoxide

[0005] 2. Insufficient awareness of the hazards of chlorpromazine, promethazine, and xylazine metabolite residues

At present, there are no regulations on the limits of metabolites of chlorpromazine, promethazine, and xylazine in my country, and there is a lack of specific research reports on related metabolites

[0006] 3. Due to the lack of detection technology, there is a lack of research on the exposure risk of chlorpromazine, promethazine, xylazine and their metabolites

At present, there are no research reports on the risk of dietary exposure to chlorpromazine, promethazine, xylazine and metabolites, but in view of the relevant case reports on acute poisoning incidents caused by chlorpromazine and xylazine in meat, this study High attention should be paid to the risk of drug residues

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