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Preparation method of novel absorbable biological membrane

A biofilm, a new type of technology, applied in tissue regeneration, medical science, surgery, etc., can solve problems such as fast degradation of recombinant barrier membranes, drug resistance of bacterial strains, and bacterial infection, so as to reduce economic and psychological burden and promote repair , the effect of reducing pain

Active Publication Date: 2021-12-24
博纳格科技(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The disadvantage of the existing technology is that the recombinant barrier membrane has the problem of too fast degradation, and the acellular matrix membrane has good biocompatibility and high clinical acceptance, but it can only play the role of physical isolation and needs to rely on the body It has the potential to repair defects by itself, has no obvious osteoinductive activity, and there is a risk of bacterial infection within one week after the material is implanted in the oral cavity. Usually, it is necessary to use antibiotics systemically within one week of GBR surgery to prevent wound infection. Systemic medication can easily lead to drug resistance of bacterial strains

Method used

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  • Preparation method of novel absorbable biological membrane

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Embodiment 1

[0026] Example 1, please refer to Figure 1-3 , the embodiment of the present invention provides a technical solution: a method for preparing a novel absorbable biofilm, comprising the following steps;

[0027] S1: Preparation of pig pericardium, use 0.1-0.5% trypsin, shake at 37°C, 80-180rpm for 2-5h, remove part of the pericardium cells of large pigs, remove telopeptide, and then wash with purified water for 6 times, Shake with 0.1%-1% SDS at room temperature 80-180rpm for 1-4h, and then wash 10 times with purified water; the porcine pericardium is composed of three layers: serosal layer, fibrous layer and interstitial layer, and the serosal layer is close to The heart is covered with a layer of mesothelial cells, and the fibrous layer is the main component of the pericardium. The collagen structure of these two layers is relatively fine, smooth and dense, and is generally called the smooth surface. The interstitial layer is connected by some connective tissue and fat. Coll...

Embodiment 2

[0040] A method for preparing a novel absorbable biofilm, comprising the following steps;

[0041] S1: Preparation of porcine pericardium, using 0.1% trypsin, shaking at 37°C and 180rpm for 3 hours, removing part of the cells in the pericardium of large pigs, removing telopeptides, and then washing with purified water for 6 times, and then using 0.1% SDS at room temperature Shake at 180rpm for 3h, wash with purified water 10 times;

[0042] S2: Preparation of the growth-promoting layer. Place the decellularized porcine pericardium with the rough side up on a plate, add heparin and bone morphogenetic protein-2 mixture, incubate at room temperature for 4 hours, and then place the incubated porcine pericardium Membranes were freeze-dried;

[0043] S3: Preparation of the gel layer, mixing polyethylene glycol, chitosan and tetracycline to prepare a gel solution after defoaming, and pouring the gel solution with the smooth side of the freeze-dried composite membrane porcine pericar...

Embodiment 3

[0051] A method for preparing a novel absorbable biofilm, comprising the following steps;

[0052] S1: Preparation of porcine pericardium, using 0.5% trypsin, shaking at 37°C and 80rpm for 3 hours, removing part of the cells in the pericardium of large pigs, removing telopeptides, and then washing with purified water for 6 times, and then using 0.5% SDS at room temperature Shake at 80rpm for 2h, wash with purified water 10 times;

[0053] S2: Preparation of the growth-promoting layer. Place the decellularized porcine pericardium with the rough side up on a plate, add heparin and bone morphogenetic protein-9 mixture, incubate at room temperature for 2 hours, and then place the incubated porcine pericardium Membranes were freeze-dried;

[0054] S3: Preparation of the gel layer. Polyvinyl alcohol, sodium alginate, and gentamicin were mixed and defoamed to prepare a gel solution. The smooth side of the freeze-dried composite membrane porcine pericardium was placed upward, and the...

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Abstract

The invention discloses a preparation method of a novel absorbable biological membrane. The novel absorbable biological membrane is composed of a three-layer structure which is divided into a pig pericardium layer, a growth promoting layer and a gel layer. The preparation method comprises the following steps that the pig pericardium layer is prepared, most cells of the porcine pericardium layer are removed by using 0.1%-0.5% trypsin, and terminal peptide is removed; then cleaning is carried out for 6 times by using purified water; oscillating is carried out for 1-4 hours by using 0.1%-1% SDS at room temperature at 80-180rpm, and cleaning is carried out for 10 times by using purified water; and the growth promoting layer is prepared, specifically, a bioactive factor solution is added into the rough surface of the decellularized porcine pericardium layer, incubating is carried out at room temperature for 2-4 hours, and then the incubated porcine pericardium layer is freeze-dried. The novel absorbable biological membrane has enough mechanical properties to support the pressure of soft tissues and prevent epithelial cells, fiber cells and the like from invading a bone defect area, and has proper thickness and porosity, so that the membrane has good fitness and comfort; and the novel absorbable biological membrane is beneficial to diffusion of oxygen, nutrient substances and bioactive substances to the tissues and promotes cell growth.

Description

technical field [0001] The invention relates to the technical field of biofilm preparation, in particular to a preparation method of a novel absorbable biofilm. Background technique [0002] It is well known that guided bone tissue regeneration is a surgical method widely used in oral implant surgery, which can effectively solve the problem of insufficient bone mass. The principle is to place a barrier membrane between soft tissue and bone defect to establish a biological barrier , to separate the fast-regenerating soft tissue from the slow-growing bone tissue, to ensure that osteoblasts have sufficient time to occupy the osteogenic space and realize the regeneration of bone tissue. The types of barrier membranes include non-absorbable membranes and absorbable membranes. Non-absorbable membranes mainly include polytetrafluoroethylene membranes (e-PTFE), titanium membranes, and paraformaldehyde membranes. However, such barrier membranes are generally made of hard materials an...

Claims

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Application Information

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IPC IPC(8): A61L31/12A61L31/06A61L31/04A61L31/00A61L31/14A61L31/16
CPCA61L31/06A61L31/042A61L31/048A61L31/005A61L31/148A61L31/14A61L31/16A61L2430/40A61L2300/412A61L2300/404A61L2300/41A61L2300/236A61L2300/252A61L2300/414C08L71/02C08L5/08C08L5/04C08L29/04
Inventor 陈辉姜智旭蒋彩云李志军
Owner 博纳格科技(天津)有限公司
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