Preparation method of temperature-sensitive chitosan hydrogel cell factor composite stent

Abstract

The invention relates to the field of biological materials, and provides a preparation method of a temperature-sensitive chitosan hydrogel cell factor composite stent. According to the technical scheme, the preparation method of the temperature-sensitive chitosan hydrogel cell factor composite stent comprises the following steps: 1) mixing chitosan with gelatin at a mass ratio of 2.5: 0.8-1.2; 2)dissolving a mixture obtained from step 1) in an acetic acid solution with the concentration being 0.1 M or an aqueous solution, and blending for 24 hours or above at the temperature of 4 DEG C; 3) dropwise adding a glycerol phosphate disodium salt solution with the concentration being 44.4% into a mixture obtained from step 2), and adding in an ultra-clean table at the temperature of 4 DEG C while stirring, wherein the mass ratio of glycerol phosphate disodium salt to the mixture is 1: 0.5-1.0; 4) dropwise adding cell factors in the mixture obtained from step 3), and adding in the ultra-cleantable at the temperature of 4 DEG C while stirring; and 5) maintaining the mixture obtained from step 4) at 4 DEG C in a liquid state, and solidifying into solid gel at 37 DEG C 10 minutes later. Thestent obtained by the method can slowly release cell factors.

Description

technical field [0001] The invention relates to the technical fields of biological materials and medical materials, in particular to a preparation method of a novel temperature-sensitive chitosan hydrogel cytokine composite scaffold. Background technique [0002] Clinically, the current treatment of corneal epithelial injury mainly uses eye drops that promote epithelial growth factor and anti-inflammatory eye drops. These traditional treatment methods have a certain effect, but the repair time is long, and the repaired epithelial structure and function are far below normal. The corneal epithelium eventually leads to irreversible scarring of the cornea, affecting the patient's vision. [0003] Chitosan material, which is mainly extracted from the exoskeleton of crustaceans and the cell wall of fungi, is an amino polysaccharide formed by deacetylation of chitin. It has many excellent biological properties: transparency, non-toxicity, low immunogenicity, good biodegradability ...

AI Technical Summary

Problems solved by technology

Moreover, cytokines have a certain biological effect period, and multiple injections in situ will be a technical difficulty in the surgical treatment of various tissues

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