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Triglyceride determination kit and determination method thereof

A technology of triglycerides and kits, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of unsatisfactory anti-interference ability and poor anti-interference ability, and achieve good measurement accuracy and enhanced stability sexual effect

Active Publication Date: 2018-12-07
广州市伊川生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are many types of stabilizers used in the existing triglyceride determination kits. Although the stability can be improved, the anti-interference ability is often not ideal, especially the anti-interference ability of drugs such as calcium dobesilate.

Method used

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  • Triglyceride determination kit and determination method thereof
  • Triglyceride determination kit and determination method thereof
  • Triglyceride determination kit and determination method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment one triglyceride assay kit (formula two)

[0024] The triglyceride determination kit includes reagent R1, which includes the following components and their concentrations: lipoprotein esterase 1KU / L, glycerol kinase 0.5KU / L, glycerol phosphate oxidase 2KU / L, peroxidase PEO -302 2KU / L, piperazine-1,4-diethanesulfonic acid 8.462g / L, NaOH 2.238g / L, 4-aminoantipyrine 61mg / L, adenosine triphosphate 0.6g / L, N-ethyl- N-(2-hydroxy-3-sulfopropyl)-3'5-dimethoxyaniline sodium salt 0.45g / L, sodium glutamate 3g / L, magnesium acetate 3.22g / L, flavin adenine di Nucleotide 3mg / L, potassium sorbate 1g / L, polyethylene glycol 6000 5g / L, betaine solution 0.6ml / L. The concentration of betaine solution is 2mol / L.

Embodiment 2

[0025] Example Diglyceride Determination Kit

[0026] The triglyceride determination kit includes reagent R1, which includes the following components and their concentrations: lipoprotein esterase 2KU / L, glycerol kinase 1KU / L, glycerol phosphate oxidase 1KU / L, peroxidase PEO- 302 1KU / L, piperazine-1,4-diethanesulfonic acid 10g / L, NaOH 4g / L, 4-aminoantipyrine 70mg / L, adenosine triphosphate 1g / L, N-ethyl-N-(2 -Hydroxy-3-sulfopropyl)-3'5-dimethoxyaniline sodium salt 0.8g / L, sodium glutamate 5g / L, magnesium acetate 3g / L, flavin adenine dinucleotide 5mg / L L, potassium sorbate 1g / L, polyethylene glycol 6000 8g / L, betaine solution 0.8ml / L. The concentration of betaine solution is 2mol / L.

Embodiment 3

[0027] Embodiment Triglyceride assay kit

[0028] The triglyceride determination kit includes reagent R1, which includes the following components and their concentrations: lipoprotein esterase 1KU / L, glycerol kinase 0.5KU / L, glycerol phosphate oxidase 4KU / L, peroxidase PEO -302 2KU / L, piperazine-1,4-diethanesulfonic acid 6g / L, NaOH 2g / L, 4-aminoantipyrine 55mg / L, adenosine triphosphate 1g / L, N-ethyl-N-( 2-Hydroxy-3-sulfopropyl)-3-methylaniline sodium salt 0.5g / L, sodium glutamate 2g / L, magnesium acetate 4g / L, flavin adenine dinucleotide 5mg / L, sorbic acid Potassium acid 1g / L, polyethylene glycol 6000 6g / L, betaine solution 0.6ml / L. The concentration of betaine solution is 2mol / L.

[0029] Comparative Example 1 Triglyceride Determination Kit (Formula 1)

[0030] The triglyceride determination kit includes reagent R1, which includes the following components and their concentrations: lipoprotein esterase 1KU / L, glycerol kinase 0.5KU / L, glycerol phosphate oxidase 2KU / L, peroxid...

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Abstract

The invention provides a triglyceride determination kit. The kit comprises a liquid single reagent R1, the reagent R1 includes the following components with concentration: lipoprotein esterase, glycerol kinase, glycerol phosphate oxidase, peroxidase, piperazine-1,4-diethanesulfonic acid, NaOH, 4-aminoantipyrine, adenosine triphosphate, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium orN-ethyl-N-(2-hydroxy-3-propyl)-3'5-dimethoxyaniline sodium salt, sodium glutamate, magnesium acetate, flavin adenine dinucleotide, polyethylene glycol, and a betaine solution. The kit belongs to the technical field of biological detection, and the triglyceride determination kit provided by the invention significantly enhances the anti-interference ability while improving the stability of the reagent, and has good measurement accuracy for triglyceride.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a triglyceride assay kit and a assay method thereof. Background technique [0002] Triglyceride (TG) is a fat molecule formed by three long-chain fatty acids and glycerol. It is the most abundant lipid in the human body, the main source of energy in the body, and an important clinical blood lipid routine determination index. Elevated triglycerides are likely to cause "blood thickening", which is not only closely related to the risk of atherosclerotic cardiovascular disease, but also closely related to the risk of microvascular complications in diabetic patients, so it has received increasing attention. [0003] The determination methods of triglyceride (TG) in serum mainly include chemical method, enzymatic method and chromatographic method. Chemical methods and chromatography methods are not suitable for widespread application in clinical routine detection due to n...

Claims

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Application Information

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IPC IPC(8): C12Q1/61C12Q1/26C12Q1/28C12Q1/44C12Q1/48
CPCC12Q1/26C12Q1/28C12Q1/44C12Q1/485C12Q1/61
Inventor 刘光华杨玉军陈楚华刘秋明
Owner 广州市伊川生物科技有限公司
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