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32results about How to "Low frequency" patented technology

Method for Cloning T Cell Receptor

An object is to provide a TCR closing system that enables not only bias-free analysis of TCR repertoires, but also collection of antigen-specific TCR α/β cDNA pairs and evaluation of functions thereof. There is provided a method for producing a gene of T cell receptor (TCR) specific to an antigen A, which comprises 1) the step of stimulating a group of T cells including a T cell specific to an antigen A or one T cell specific to an antigen A under a condition effective for amplification of a TCR gene; 2) the step of identifying a T cell specific to an antigen A among the group of T cells including a T cell specific to the antigen A, and sorting one T cell specific to the antigen A into a vessel; and 3) the step of subjecting the one activated T cell specific to the antigen A in the vessel to PCR to amplify a gene of TCR specific to the antigen A. According to the present invention, a target TCR gene can be cloned within a shorter time compared with that repaired by the conventional methods, for example, about ten days. Further, according to the present invention, genes of TCR α chain and β chain can be highly efficiently cloned. Under the conditions of the examples, a pair of a TCR α chain and TCR β chain could be obtained from stimulated T cells sorted as single cells at a ratio of 100%.
Owner:UNIVERSITY OF TOYAMA

Broadband active inductor with high Q values and coarsely tunable and finely tunable inductance value

The invention discloses a broadband active inductor with high Q values and a coarsely tunable and finely tunable inductance value. The broadband active inductor comprises a first transconductance unit (1), a modulation unit (2), a second transconductance unit (3), a third transconductance unit (4), a fourth transconductance unit (5), a first tunable bias circuit (6), a second tunable bias circuit (7), a third tunable bias circuit (8) and a fourth tunable bias circuit (9). The modulation unit is connected with the first transconductance unit and is used for increasing the Q values and bandwidth of the active inductor. The first transconductance unit, the modulation unit and the second transconductance unit form a master circuit. The third transconductance unit and the fourth transconductance unit form a slave circuit. The master circuit and the slave circuit are connected in parallel. The equivalent capacitance for synthesizing the inductance in the whole circuit is increased, so the inductance value of the active inductor is improved. According to the active inductor, the high Q peaks of the active inductor under different frequencies are realized, the Q peaks can be tuned, and the inductance value of the active inductor is coarsely tuned and finely tuned.
Owner:BEIJING UNIV OF TECH

Quick detecting method for harmonic wave of electric power system

The invention discloses a quick detecting method for a harmonic wave of an electric power system, and particularly relates to a method for detecting phase and amplitude of signals with different frequencies in the electric power system. The method comprises the following steps of (1), acquiring sampling points of which the number is at least two times of that of the harmonic waves through using two times of highest multiplied frequency as a sampling rate; (2), constructing a sine signal and a cosine signal which correspond with each harmonic wave frequency; (3), calculating a harmonic wave detecting parameter through fitting the coefficient before each sine item and cosine item; and (4), combining the coefficients in front of the sine item and the cosine item with the same frequency for obtaining the amplitude and the phase of the harmonic wave with each frequency. Compared with the prior art, the quick detecting method is advantageous in that a small volume of data are required; a result can be calculated just through the data with length of one half of that of a fundamental frequency signal; relative to an FFT (fast Fourier transform) method, a problem of frequency leakage can be prevented; and the method is compatible with clutter interference to a certain extent.
Owner:CHINA UNIV OF MINING & TECH (BEIJING) +2

Ultrahigh frequency metal RFID signboard

The invention discloses an ultrahigh-frequency metal RFID (radio frequency identification) signboard, which sequentially comprises a metal signboard, a first double-faced adhesive tape layer, an antenna grounding layer, a second double-faced adhesive tape layer, an insulating medium layer, a third double-faced adhesive tape layer, an antenna chip layer and a fourth double-faced adhesive tape layer from top to bottom, and is characterized in that the metal signboard is adhered to the antenna grounding layer through the first double-faced adhesive tape layer; the antenna grounding layer and the antenna chip layer are separated by filling an insulating medium layer, the antenna chip layer is adhered to a managed object through a fourth double-faced adhesive tape layer, the microstrip antenna is characterized in that the antenna grounding layer and the antenna chip layer are conducted through a side face conduction layer to form a microstrip antenna structure, a chip is arranged at the bottom of the microstrip antenna structure, and the antenna grounding layer and the antenna chip layer are connected through a second double-faced adhesive tape layer. And the metal signboard is conducted with the antenna grounding layer at the upper part of the microstrip antenna structure. By means of the mode, holes or excitation holes do not need to be formed, the integrity of the metal layer is guaranteed, and meanwhile the impact resistance of the metal layer is greatly improved.
Owner:泰芯智能科技(昆山)有限公司

Buffering device

The invention provides a buffering device. The buffering device comprises a shell, a plurality of buffering pipes, an elastic part, a supporting piece and an elastic supporting part; a plurality of similar annular grooves are formed in the hole walls of the buffering pipes, and a plurality of gaps formed in the axial directions of the buffering pipes are formed in the buffering pipes; the elastic part is arranged in the accommodating cavity; a plurality of ring-like bulges are arranged on the outer wall surface of each supporting column; the elastic supporting part is arranged in a connecting hole and located between the shell and the supporting columns; each supporting column comprises a connecting section, a protruding section and a supporting tail section which are sequentially connected; and the lengths of the supporting tail sections of the multiple supporting columns are at least two. According to the buffering device, due to the fact that the lengths of the supporting tail sections in the supporting columns are different, the protruding sections on the different supporting columns have phase differences; the resistances borne by the different supporting columns are different; therefore the load-displacement curve of the structure is changed through the combination of the different supporting columns; and the specific damping coefficient of the buffer device is greatly improved to enhance the buffering performance of the structure.
Owner:HARBIN INST OF TECH
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