Preparation method of gel containing stem cell exosomes for repairing skin wounds

A technology for skin wounds and exosomes, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve the problems of clinical scale application limitations, complicated collagen processing technology, difficult to control degradation rate, etc., to achieve inhibition The formation of scar tissue, the shortening of wound healing time, and the effect of improving the repair effect

Inactive Publication Date: 2020-07-17
陕西朗泰生物科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

Collagen is one of the main components of the human extracellular matrix, and is generally considered to be an ideal biomaterial for wound dressings. However, the complex processing technology of collagen and the difficulty in controlling its degradation rate have brought certain limitations to clinical scale applications.

Method used

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  • Preparation method of gel containing stem cell exosomes for repairing skin wounds
  • Preparation method of gel containing stem cell exosomes for repairing skin wounds
  • Preparation method of gel containing stem cell exosomes for repairing skin wounds

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Embodiment Construction

[0044] The steps of the specific embodiment of the present invention are as follows:

[0045] 1) Primary extraction and culture of human umbilical cord mesenchymal stem cells:

[0046] 1.1) Primary extraction of human umbilical cord mesenchymal stem cells: the umbilical cord tissue was taken from a full-term healthy newborn. After the tissue was sterilized with 75% alcohol for 30 seconds, Wharton’s jelly was stripped and cut into 0.5cm 3 The centrifugation condition is room temperature, 200-300g speed centrifugation for 5-8min, after centrifugation, in the culture medium in an incubator at 37°C and 5% CO2; replace the medium carefully every 3 days, about 12 After ~14 days, the umbilical cord tissue was removed, and the adherent cells were routinely cultured;

[0047] 1.2) Subculture: When the confluence of the adherent cells reaches 80%, the cells are digested with 0.25% trypsin-EDTA and subcultured;

[0048] The culture medium for primary and subculture cells is α-MEM mediu...

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Abstract

The invention relates to a preparation method of a gel containing stem cell exosomes for repairing skin wounds. The method comprises the following steps: 1) primary extraction and culture of human umbilical cord mesenchymal stem cells: 1.1) primary extraction of human umbilical cord mesenchymal stem cells, 1.2) subculture, and 1.3) collection of the culture supernatant; 2) extraction of human umbilical cord mesenchymal stem cell exosomes: 2.1) primary centrifugation, 2.2) secondary centrifugation, 2.3) removal of organelles by centrifugation, 2.4) coarse extraction of exosomes, and 2.5) finalextraction of exosomes; 3) preparation of a gel material: 3.1) preparation of chitosan, 3.2) configuration of beta-glycerol phosphate (beta-GP), and 3.3) preparation of the gel material; and 4) gel loading of the exosomes. The gel containing stem cell exosomes can promote repair of skin wounds, shorten the healing time of wounds and reduce scar formation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing a gel containing stem cell exosomes for repairing skin wounds and an application thereof. Background technique [0002] The repair of skin and soft tissue wounds is a multi-stage process involving factors such as inflammation, proliferation, and tissue remodeling, and each stage is distinct and overlaps with each other. The growth factors, cytokines, chemokines and various cells involved in this process interact in complex and delicate ways. However, an imbalance in any of these stages and factors may lead to the disruption of the coordination of the normal wound healing process, eventually leading to the regression of chronic non-healing wounds and the formation of abnormal scars. For example, in conditions such as diabetes, infection or radiation exposure, disruptions in the body's cellular and molecular signaling can trigger protracted or chroni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00C12N5/0775
CPCA61L26/00A61L26/0023A61L26/0057A61L26/0066A61L2300/412A61L2300/602C12N5/0665C12N2500/02C12N2523/00C08L5/08
Inventor 胡储涵
Owner 陕西朗泰生物科技有限公司
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