The invention relates to a clinical nutrition formula special for female sterility. The clinical nutrition formula contains proteins, amino acid, fat, carbohydrate, dietary fiber, macroelements, microelements, vitamins, dietary essences, medical-edible components, natural plant compounds and new resource food, wherein the dietary essences include black bean powder; the medical-edible components are selected from at least one of mint, poria, liquorice, rhizoma polygonati, chrysanthemum, dandelion, Chinese yam, Chinese dates, Houttuynia cordata, platycodon grandiflorum, honeysuckles and radix puerariae. The preparation method comprises the steps of grinding the components into powder, and preparing corresponding dose forms, wherein the medical-edible components are prepared into powder through water extraction, concentration and spray drying, and the vitamins and the fat are prepared into powder through microencapsulation. Compared with the prior art, the clinical nutrition formula is capable of providing comprehensive nutrition support to patients with female sterility, so that the malnutrition state is improved; meanwhile, by adding the medical-edible components having the efficacies of nourishing kidney, tonifying spleen and soothing liver, the pregnancy of the patients can be promoted.