Circulating tumor cell isolation and enrichment device based on closed loop

Abstract

The present invention discloses a circulating tumor cell isolation and enrichment device based on a closed loop. The circulating tumor cell isolation and enrichment device based on the closed loop includes a top plate; two ends of the bottom of the top plate are respectively fixedly connected with a left bracket and a right bracket located at the right side of the left bracket; the left side of the right bracket is fixedly provided with a device main body which is of a hollow structure; the bottom of the device main body is provided with a sample outlet where a liquid collecting bottle is disposed, and a filter membrane is further disposed between a top bottleneck of the liquid collecting bottle and the sample outlet; the inside of the device main body is movably provided with an oscillation plate; and the top of the oscillation plate is fixedly provided with a lifting bar and a toothed bar located at the right side of the lifting bar. In the circulating tumor cell isolation and enrichment device based on the closed loop, the activity of a sample liquid in the device main body may be accelerated, the disadvantage that an original liquid is relatively still and relatively low in flowability and activity is changed, and the isolation of the sample liquid and the filter membrane in a cell enrichment device may be further accelerated, so that the tumor cell isolation and enrichmentefficiencies are greatly increased, and the use demands of people are met.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a device for separating and enriching circulating tumor cells based on a closed loop. Background technique [0002] For the purpose of medical diagnosis and treatment, it is necessary to screen and separate specific target cells from human or animal blood, tissue fluid or lymph fluid, especially blood, mainly diseased cells or cells exfoliated from diseased tissues and organs . Frequently, the target cells are present in very rare quantities in the aforementioned body fluids. [0003] For example, circulating epithelial cells are a kind of rare cells present in the blood, which are exfoliated from diseased epithelial tissues, among which those exfoliated from benign lesion tissues are benign epithelial cells, and those exfoliated from malignant lesion tissues (cancer) are tumor cells. It is extremely rare, and every 10ml of blood may only contain a few to dozens of circ...

AI Technical Summary

Problems solved by technology

[0009] However, in the existing technologies for the separation and enrichment of circulating tumor cells in a closed loop, due to the relative prohibition, low fluidity and low activity of the sample liquid in the cell enrichment device, the sample liquid in the cell enrichment device and the filter membrane The speed in the separation process is slow, which seriously affects the efficiency of cell separation and enrichment, and needs to be greatly improved. For this reason, the present invention proposes a circulating tumor cell separation and enrichment device based on a closed loop to solve the above-mentioned problems. question

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