76 results about "Filtration diaphragm" patented technology

The diaphragm one-way valve type bacteria-blocking filter comprises a bacteria-blocking filter membrane, a T-shaped rubber ring is arranged on the outer side of the bacteria-blocking filter membrane in an injection molding mode, an air hole penetrating through the T-shaped rubber ring is axially formed in the T-shaped rubber ring, the bacteria-blocking filter membrane is transversely arranged in the large-diameter end of the T-shaped rubber ring and blocks the air hole, and the air hole is communicated with the T-shaped rubber ring. An installation groove is formed in the T-shaped rubber ring in a downward extending mode, a one-way diaphragm is arranged in the installation groove and comprises a positioning ring, an elastic blocking piece and an elastic connecting wire, the positioning ring, the elastic blocking piece and the elastic connecting wire are integrally formed, the positioning ring is clamped in the installation groove, the elastic blocking piece is attached to the bottom wall of the T-shaped rubber ring in a zero-pressure mode and blocks an air hole, and the elastic connecting wire is connected with the T-shaped rubber ring. And the elastic connecting wires are circumferentially and uniformly distributed between the positioning ring and the elastic plugging sheet. The liquid dropping bottle has the advantages that the bacteria-blocking filter membrane is not in contact with liquid medicine in the liquid dropping bottle any more, so that membrane holes in the bacteria-blocking filter membrane cannot be blocked, the hydrophobicity of the bacteria-blocking filter membrane cannot be damaged, and complete hydrophobicity and air permeability are kept.

The filtration element (1) includes a filtration membrane (3) enclosed within a housing (2). The element has openings for removing filtrate and concentrate. A filter (11) is disposed within the element (1) at an opening (6) in the housing (2) for introducing feed into the interior volume (20) of the filtration element (11). Alternatively, the filter (11) may be positioned within an end cap (7) associated with such opening. Pre-filtration is thus performed within the filtration element 1 itself, rather than as a separate upstream operation. The presence of the filter (11) does not significantly increase the pressure drop across the filtration module. Flocculated particles and other debris that accumulate on the membrane can be easily removed by backwashing, despite the presence of the filter (11).

The present application discloses a hemoperfusion device, which includes a cylinder body. A filter element assembly is arranged inside the cylinder body. A flexible blood bag is arranged at the top of the cylinder body. A flexible liquid bag is arranged inside the cylinder body and is internally connected to the filter element assembly. The volume of the flexible liquid bag can change under the action of an external force. A filter membrane assembly internally connected to the filter element assembly is arranged inside the flexible blood bag. An inlet and an outlet are arranged on the flexible blood bag. Two one-way valves arranged in the same direction are respectively arranged on both sides of the filter membrane assembly. A partition is arranged inside the blood bag. A reflux channel is formed between the partition and the filter membrane assembly, and the interior of the blood bag is divided into a first inner cavity and a second inner cavity. After the blood enters the first inner cavity through the inlet, it flows into the cylinder body from one one-way valve. After the blood is filtered by the filter membrane assembly and the filter element assembly, it flows to the second inner cavity from the other one-way valve. Part of the blood flows out through the outlet, and part of the blood can flow back to the first inner cavity through the reflux channel.

The utility model discloses a container shell for a blood filter, which is characterized in that a second shell is inserted into one side of a first shell, a volume cavity is formed by the first shell and the second shell, and a filter membrane is arranged on the inner side of the volume cavity; a first flow guide structure is arranged on the inner wall of the first shell, a second flow guide structure is arranged on the inner wall of the second shell, and the first flow guide structure and the second flow guide structure guide liquid in the volume cavity; according to the device, the flow channels of the liquid inlet and the liquid outlet are additionally arranged, the flow guide structures are arranged on the two sides of the flow channels, blood enters the liquid inlet, flows out of the first flow channels and diffuses to the upper surface of the whole filter membrane along a path formed by the first flow guide structures arranged in the first shell in parallel, the blood filtering path is prolonged, the filter membrane and the edge part are fully utilized, and the blood filtering efficiency is improved. The second flow guide structure is arranged continuously or discontinuously and corresponds to the position of the part, with the maximum blood flow, of the liquid inlet.

The utility model relates to the technical field of serum extraction, in particular to a serum filtering device which comprises a base plate, a protective sleeve is arranged above the test tube, a hydraulic rod is fixedly connected to the interior of the protective sleeve, a connecting plate is fixedly connected to the outer side of the free end of the hydraulic rod, a cover plate is fixedly connected to one end of the connecting plate, a placement groove is formed in the center of the cover plate, and the cover plate is spirally connected with a pressure applying mechanism through the placement groove. According to the device, a flat plate filter membrane is cut and placed above the protective sleeve, the hydraulic rod is started to drive the connecting plate and the cover plate to move, the pressing ring below the cover plate can be matched with the annular groove to achieve the purpose of fixing the filter membrane, an injector for extracting materials is placed in the pressure applying mechanism, the pressure applying mechanism is connected with the cover plate, and then the filter membrane is fixed. And the pressure applying mechanism is started to apply pressure to the injector, so that the material can be conveniently pressurized and filtered, and a small amount of material / serum can be filtered even only 5-10ml.

The utility model discloses a low energy consumption water purification device. The low energy consumption water purification device includes the filter mechanism for filtering, the desalination mechanism for desalination and the disinfection mechanism for disinfection, the desalination mechanism includes main desalination pipe, vice desalination pipe, diaphragm pump, nanofiltration membrane component, membrane capacitance deionization device and valve component for the water flow into membrane capacitance deionization device or vice desalination pipe of alternative control, the filter mechanism and disinfection mechanism are connected through main desalination pipe, diaphragm pump, nanofiltration membrane component and membrane capacitance deionization device are installed in sequence on main desalination pipe, vice desalination pipe is provided with on main desalination pipe in parallel with membrane capacitance deionization device. The low energy consumption water purification device of the utility model can select desalination path according to TSD, avoids unnecessary equipment starting, and saves energy consumption.

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

This invention relates to the construction and application of an exosome purification device. The purification device utilizes the differences in size and charge between exosomes and other substances, combining the tangential flow of the sample within the device with the dual effects of membrane sieving and an electric field to remove contaminants and achieve high-purity exosome enrichment. The device includes a pre-filtration section and an exosome purification section. This device is simple to operate, can handle large sample volumes, has a short processing time, is less prone to membrane clogging by contaminants, and achieves high purity exosome separation with minimal structural damage. It can meet the separation and purification needs of exosomes from different sources and can be further applied to research on exosome proteomics, drug loading, activity, and biological functions.

The invention provides a cell product production instrument and a cell product production method using the same. The cell product production instrument comprises a whole blood sample bag; a magnetic separation device; the target cell processing module comprises a module main body, an accommodating cavity is formed in the module main body, a filter membrane is arranged in the accommodating cavity, the accommodating cavity is divided into a first space and a second space by the filter membrane, and a transfection reaction cavity capable of accommodating target cells is formed in the bottom of the first space; a heating module; a sample pumping assembly; the cleaning fluid pumping assembly is used for pumping the cleaning fluid in the cleaning fluid bag into the first space through the cleaning fluid inlet; the reagent pumping assembly is used for pumping a transfection reagent and / or a culture medium into the first space through the reagent inlet; and the cell product pumping assembly is used for conveying the transfected target cells in the first space into the finished blood bag through the cell product outlet. According to the invention, manual intervention and process breakpoints are thoroughly eliminated by realizing full-automatic internal circulation among the modules.

The present invention relates to the field of exosome extraction. Disclosed are a low-temperature exosome extraction device and a method for using same. The low-temperature exosome extraction device comprises an extraction stand and a bearing platform fixed on the extraction stand, and further comprises a plurality of filter cylinders mounted on the bearing platform at equal angular intervals, a collection cylinder circumferentially slidably mounted on the extraction stand, a vacuum tube connected to the collection cylinder, and liquid intake tubes fixed on the extraction stand and used for adding an auxiliary exosome extraction reagent into the filter cylinders, wherein a sliding block is slidably mounted on the side wall of the collection cylinder, a connecting ring for connecting and sealing the collection cylinder and a filter cylinder is fixed on the sliding block, and a steering support is fixedly connected to the collection cylinder; and the extraction stand is provided with a steering assembly for controlling the collection cylinder to rotate to the position under different filter cylinders. The low-temperature exosome extraction device simplifies the extraction structure, and, during multiple filtration for exosome extraction, agitates and disperses a cell fluid to be filtered, thereby preventing macromolecular exosomes from clogging a filter membrane, and improving the purity of extracted exosomes.

The invention discloses a large-scale high-quality plasmid extraction and purification process, which belongs to the technical field of molecular biology and mainly comprises the following steps: thallus cracking, solid-liquid separation and clarification, concentration and liquid exchange, plasmid purification and quality inspection and warehousing. Wherein the plasmid purification step comprises the following steps: adding an ammonium sulfate solid into a plasmid extraction concentrated solution, and then centrifuging. And centrifuging, recovering the centrifuged supernate, filtering by using a 3-micron filter membrane, then carrying out ultrafiltration concentration by using a 10-750KDa membrane bag, adding pure water, continuing to concentrate and change the liquid, and thus obtaining the purified plasmids. According to the method, plasmids are purified by adopting a one-step salting-out method, the plasmid purification step can be completed only in 1-2 h, compared with an existing column chromatography method, the purification time can be shortened, the purification cost can be reduced, and the method is suitable for obtaining different plasmids.

The utility model relates to a suspension cell culture bottle capable of improving ventilation efficiency, which is characterized by comprising a culture bin for cell culture and two ventilation holes positioned on the short side of the top. The air hole is located in the other side of the bottle opening I and the bottle opening II. The culture bottle is large in bottom area and low in height, the volume of the culture bottle is increased by increasing the bottom area of the culture bottle, meanwhile, a liquid-gas interface of a solution is increased along with the increase of the bottom area of the culture bottle, and the gas exchange efficiency of a cell culture solution is greatly improved. Except a conventional gas exchange way through a hydrophobic filter membrane at a bottle opening, the two air vents of the culture bottle provide an additional gas exchange way, so that the culture bottle can still provide enough gas exchange under the condition of culturing a large amount of cell suspension.

The application discloses a hemostasis valve assembly and a device for delivering a delivery system, and applies to the technical field of medical devices, and has the technical scheme as follows: a head end hemostasis part comprises a hollow circular table provided with a first cavity, and a first hemostasis channel is arranged on the head side of the hollow circular table; a blocking filter membrane is installed on the tail side of the hollow circular table and blocks the first cavity, and a second hemostasis channel is arranged on the blocking filter membrane; a middle end hemostasis part comprises a ring-shaped cylinder provided with a second cavity, and the head end of the ring-shaped cylinder is in abutment with the blocking filter membrane; a tail end hemostasis part is sleeved on the tail part of the ring-shaped cylinder and is used for blocking the second cavity, a third hemostasis channel is arranged on the tail end hemostasis part, the first hemostasis channel, the second hemostasis channel and the third hemostasis channel are arranged along the same axis, and when a penetrating object is withdrawn in a reverse order, the blocking filter membrane moves to the tail side direction to make the pressure of the second cavity greater than the pressure of the first cavity. The application can reduce the occurrence of blood leakage and achieve a good blood sealing effect.

The utility model discloses a plasma separation and rapid detection integrated device which comprises a storage tank, a limiting ring is fixedly installed on the inner side of the upper end of the storage tank, a containing tank is installed on the inner side of the limiting ring, a heating block is installed at the bottom end of the containing tank, a plurality of EP pipes are installed on the inner side of the containing tank, and a heating block is installed at the bottom end of the containing tank. A sealing film is arranged on the upper end face of the EP pipe. A connecting sleeve is mounted on the outer side of the upper end of the storage tank, a pressurizing cover is mounted on the inner side of the upper end of the connecting sleeve, an elastic connecting belt is arranged on one side of the connecting sleeve, a filtering mounting sleeve is mounted on the lower end face of the pressurizing cover, and a double-layer plasma separation filter membrane is mounted on the inner side of the bottom end of the filtering mounting sleeve. Collected blood is immediately pressurized and filtered, and meanwhile, the EP tube filled with a plasma rapid detection reagent can be used for constant-temperature storage, so that blood sample pollution, hemolysis possibly caused in the normal-temperature transportation process of a whole blood sample and the like are effectively avoided.

The invention relates to the technical field of detection, in particular to a microfiltration device for alpha fetoprotein heteroplasmon detection, a limiting baffle ring is arranged at the top of a supporting head in a microfiltration head component, corresponding limiting is provided for placement of a filter membrane, and the filter membrane is in contact with the inner side wall of the limiting baffle ring, so that a gap is prevented from being generated, and the detection accuracy of alpha fetoprotein heteroplasmon detection is improved. The working efficiency of filtering and adsorbing the alpha fetoprotein heterogeneous is improved, and the positive effect is achieved on the accuracy of subsequent detection work; an auxiliary assembly is further arranged in the supporting head part, so that the filter membrane can be better taken out, and the phenomenon that the filter membrane is easily damaged when the filter membrane is clamped by tweezers from the middle part of the filter membrane is avoided; in addition, an auxiliary pressing ring in the arranged volume cup assembly also has an extrusion limiting effect on the filter membrane, namely the problem that the filter membrane shakes when liquid is poured in is avoided, and the working stability of the filter membrane is improved; and the method has positive significance in improving the detection and filtering work efficiency of the alpha fetoprotein variant.

This invention discloses a hemodialysis coagulation inhibition device based on porous modified materials, relating to the field of dialysis technology. It includes a housing with an upper cover and a lower cover fixedly installed at its upper and lower ends, respectively. An exchange chamber and an expansion chamber are interconnected within the housing. A porous modified material filter element is installed within the exchange chamber, with its bottom fixed to the housing and its top floating within the exchange chamber. An overflow pipe extending into the expansion chamber is fixedly installed on the exchange chamber. A Venturi drain pipe is fixedly installed at the bottom of the expansion chamber. This invention achieves mainstream bubble separation of the dialysate through a spiral guide plate, and the micro-negative pressure formed by the Venturi drain pipe and the suction pipe continuously removes gas from the expansion chamber. The porous filter membrane filaments oscillate, disturbing the flow field and peeling away microbubbles in dead corners of the membrane surface. These three elements work together to achieve deep degassing throughout the entire area. Furthermore, the reduction in bubbles reduces membrane surface deposits, further assisting in anticoagulation.

This utility model provides an ultrafiltration centrifuge tube, including an inner tube and an outer tube. The outer edge of the inner tube's opening overlaps the inner edge of the outer tube's opening. The gap between the outer wall of the inner tube and the inner wall of the outer tube forms a filtration cavity. The outer tube's port is provided with a cap. The upper end of the inner tube has a liquid storage cavity, and the lower end has a filtration cavity. A wedge-shaped block is provided in the filtration cavity. The inner tube has a first filtration surface and a second filtration surface arranged opposite each other on both sides of the filtration cavity. Both the first and second filtration surfaces are provided with filter membranes. The wedge-shaped block is located between the first and second filtration surfaces, dividing the filtration cavity into the first and second filtration cavities. The filter membrane is an ultrafiltration membrane, and the wedge-shaped block is wedge-shaped.

The invention discloses a method for capturing, identifying and culturing circulating tumor cells of sarcoma in vitro. The method comprises a step of filtering a peripheral blood sample of a sarcoma patient by using a flexible microfiltration membrane, and cells obtained by filtration can be directly subjected to multiplication culture on the filtration membrane. Immunomagnetic beads of a monoclonal antibody combination group coupled with the anti-human leukocyte surface antigen CD45 can be used for negative enrichment and then multiplication culture is carried out in a cell culture bottle, and the captured or cultured cells can be subjected to subtype identification, characterization and counting by adopting an iFISH technology. It is found for the first time that the flexible microfiltration membrane can be used for capturing and culturing the sarcoma circulating tumor cells, and the flexible microfiltration membrane has no toxic effect on the sarcoma circulating tumor cells. The invention not only provides an effective means for real-time monitoring of relapse and metastasis processes of sarcoma patients and chemotherapy drug resistance monitoring, but also can perform drug sensitivity experimental verification on CTC after multiplication culture, and provides a powerful weapon for accurate diagnosis and treatment of sarcoma.

The utility model discloses a filter device for preparing a flow antibody, which comprises a filter component, the filter component comprises a filter membrane, an outer packing material and a fixing rod, the outer packing material comprises a first outer packing material and a second outer packing material, the filter membrane is arranged between the first outer packing material and the second outer packing material, the first outer packing material is provided with an input port and an output port, and the fixing rod is arranged on the first outer packing material. The filter membrane comprises an input surface and an output surface, the input port is communicated with the input surface, the output port is communicated with the output surface, fixing through holes are formed in the filter membrane and the outer packing material, and the filter membrane and the outer packing material are fixedly mounted through the fixing through holes and fixing rods; according to the present invention, by using the tangential flow principle, the large and small molecules can be effectively separated so as to improve the quality of the finished product, and by using the fixing rod and the through hole to mount and fix the filtration membrane and the outer packing material, the filtration assembly can be easily disassembled so as to easily clean, and the purposes of repeated use and cost reduction can be achieved.

The utility model relates to the technical field of biology, in particular to a U-shaped tube cell separation device which comprises a bottom plate, a top plate is arranged at the upper end of the bottom plate, a negative pressure extraction mechanism is arranged on one side, located on the top plate, of the bottom plate, a lifting mechanism is arranged on the other side of the bottom plate, a containing groove is formed in the top plate, and a U-shaped cell filter tube is arranged in the containing groove. And the bottom of the U-shaped cell filter tube is supported by a lifting mechanism. The U-shaped tube cell separation device has the advantages of being simple in structure, convenient to operate, high in cell separation efficiency, small in cell damage and the like, can be suitable for separation of various types of cells through different microfiltration membranes, and meets the requirements of the fields of biological experiments, cell therapy and the like.

The invention relates to the technical field of filtering devices, in particular to a medical filter and a preparation method thereof.The filter comprises a flow dividing assembly, a liquid inlet filter membrane and a liquid outlet filter membrane; wherein the liquid inlet filter membrane and the liquid outlet filter membrane are arranged on the two sides of the flow dividing assembly respectively, and a first liquid passing cavity supported by the flow dividing assembly is formed; the liquid inlet filter membrane and the liquid outlet filter membrane are arranged on the two sides of the flow dividing assembly respectively, a two-stage filter channel is formed, and liquid medicine is primarily filtered through the liquid inlet filter membrane, then evenly dispersed through the cavity supported by the flow dividing assembly and finally secondarily filtered through the liquid outlet filter membrane; a double-membrane structure is used for filtering twice, so that the impurity interception capability can be remarkably enhanced, and meanwhile, the stability of the filter during intermittent working can be effectively improved.

The utility model discloses a colloidal gold test paper card for rapidly determining rice yeast acid in whole blood. The colloidal gold test paper card comprises a bottom plate as well as a glass cellulose membrane, a filtration binding area, a detection area and a water absorption pad which are sequentially arranged on the bottom plate from left to right, the filtration binding area adopts an antigen-antibody binding technology, red blood cells in whole blood can be retained on a filter membrane of a sample pad without moving, other substances in the whole blood can smoothly pass through, and the detection effect is good. According to the present invention, the kit can be combined with the rice yeast acid so as to eliminate the background interference, can be combined with the rice yeast acid, and can be detected through the detection region, such that the rice yeast acid in the whole blood can be accurately detected, the accuracy and the specificity of the rice yeast acid determination in the whole blood can be effectively improved, the application range and the efficiency of the trace whole blood sample in the detection can be expanded, and the redundant pretreatment operation is not required; the method is simple in operation and rapid in detection, and can obtain a result within 15 minutes only by taking 10 microliters of trace whole blood, and the lowest detection limit of 50 micrograms / L is reached.

The application provides a cell product production instrument and a cell product production method using the same. The cell product production instrument comprises: a whole blood sample bag; a magnetic sorting device; a target cell processing module comprising a module main body, a containing cavity is formed in the module main body, a filter membrane is arranged in the containing cavity, the filter membrane separates the containing cavity into a first space and a second space, and the bottom of the first space is provided with a transfection reaction cavity capable of containing target cells; a heating module; a sample pumping assembly; a cleaning liquid pumping assembly for pumping cleaning liquid in a cleaning liquid bag into the first space through a cleaning liquid inlet pump; a reagent pumping assembly for pumping transfection reagents and / or culture medium into the first space through a reagent inlet pump; and a cell product pumping assembly for conveying the target cells in the first space after transfection into a finished product blood bag through a cell product outlet. The application completely eliminates manual intervention and process breakpoints by realizing full-automatic internal circulation among the modules.

To provide a filtration device which can increase of an amount of a filtrate.SOLUTION: A filtration device includes a spiral membrane module 10. The membrane module includes: an element in which a filtration membrane is wound around a winding axis extending in a predetermined direction; and a container which houses the element. The container includes a first container 110 and a second container 210 disposed at one side in the predetermined direction of the first container. The element includes: a first element 120 housed in the first container; and a second element 220 housed in the second container. The first container includes: a first outlet 114b of a filtrate, and the second container includes a second outlet 214b of the filtrate.SELECTED DRAWING: Figure 3

The utility model provides an ultrafiltration concentration tube device with a sterile filtration function, which solves the problems that the existing ultrafiltration tubes with different specifications and models can only achieve the purpose of protein concentration, different proteins can be confused due to frequent operation after the concentration is finished, the recovery rate is reduced, the risk of introducing bacteria is increased and the like. According to the main scheme, a tube body internally provided with a cavity is included, the top of the tube body is open, the tube body can be sealed into a whole through a tube cover, the tube body is formed by splicing three sections, the adjacent splicing positions are fixed through deformation friction, and the three sections of the tube body sequentially comprise an antibody storage area, a liquid storage area and a liquid discharging area from the upper end opening to the lower end opening; a first supporting table and a second supporting table are fixed to the two sequentially-connected positions of the inner wall of the pipe body respectively, a sterile filter membrane is embedded in the first supporting table, and an ultrafiltration membrane is embedded in the second supporting table.

The invention discloses a filter used in a hemodialyzer, and relates to the field of hemodialyzers, the filter comprises a shell sleeve, a middle plate rack, a flow guide unit, a membrane cleaning unit and a control unit, the membrane cleaning unit and the flow guide unit can move to a first position and a second position under the control of the control unit, the first position is a working position where the membrane cleaning unit is separated from the filtering channel and the flow guide unit is overlapped with the filtering channel, and the second position is a membrane cleaning position where the membrane cleaning unit is overlapped with the filtering channel and the flow guide unit is separated from the filtering channel. The hemodialysis filter membrane for hemodialysis filtration is arranged in the shell sleeve, and the shell sleeve is internally compatible with the membrane cleaning unit capable of regularly cleaning the hemodialysis filter membrane, so that when the device is actually used, the replacement frequency of the hemodialysis filter membrane is reduced, and the replacement efficiency of the hemodialysis filter membrane is improved. The membrane cleaning unit is used for regularly cleaning the membrane, so that the use cost of the equipment is reduced, and the downtime of the equipment can be reduced.

The invention belongs to the technical field of biological medicine, and particularly relates to gradient centrifugation leukocyte extraction equipment and method for inhibiting release of inflammatory factors, the equipment comprises a centrifugal cavity with a cover body, a placing cavity is arranged in the cavity body, the placing cavity is equipped with an illuminating lamp, an image sensor, a temperature sensor, a cooling assembly and a heating assembly, and the bottom is connected with a centrifugal driving assembly; the supporting frame at the top end of the placement cavity is matched with the supporting block, and the equipment stability and the sample treatment effect are improved by combining microfiltration membranes and other structures. According to the extraction method, the equipment is utilized, through the four steps of equipment pre-adjustment, sample preparation and placement, centrifugal process synchronous adjustment (including photo-biological adjustment, precise temperature control, gas signal molecule intervention and dynamic monitoring feedback) and leukocyte extraction, inflammatory factor release is effectively inhibited while gradient centrifugal separation is performed, the leukocyte extraction quality and activity are improved, and the leukocyte extraction efficiency is improved. The method is suitable for efficient extraction and subsequent research application of leukocytes in clinical blood samples.

Traditional hemofiltration devices can include a linear blood channel, a linear dialysate channel, or both. Additionally, the blood channel or dialysate channel can be modified so that one region has a recess in a channel wall and a filtration membrane can be positioned along the edge of the recess. However, due to fluid dynamics, some blood or dialysate could linger within the recess by moving slowly. Therefore, other fractions of the blood or dialysate would simply bypass the recess and filtration membrane by flowing through the center of the channel. This flow pattern would reduce filtration efficiency since some blood or dialysate remains far from the filtration membrane. However, positioning a flow diverter adjacent to the recess could force most or all of the blood or dialysate into the recess, thereby increasing filtration efficiency. In other words, the flow diverter would modify the fluid dynamics in a manner that would reduce the dead space in the blood channel or dialysate channel and increase filtration efficiency.

The invention relates to the technical field of cell culture, and particularly discloses a pancreas islet cell culture device in pancreas islet transplantation, which comprises an outer bracket, the outer bracket comprises a bottom plate, two shells are fixedly connected above the bottom plate, two moving assemblies are mounted in the shells, one side of each moving assembly is provided with a cam assembly, and the other side of each moving assembly is provided with a cam assembly; a cam assembly is installed on the top of the moving assembly, a motion sealing assembly is installed on one side of the cam assembly, a material receiving weight measuring assembly is installed at the bottom of the moving assembly, and a filter membrane assembly is movably connected to the top of the moving assembly. By utilizing the characteristic that gas and spray particles can pass through the fluidity of gas and the size of a gap in the film, the effect that a nutrient solution can be provided without opening a cover in the islet cell culture process is realized, the possibility that bacteria enter a culture dish in the culture process is greatly reduced, the environmental purity of islet cell culture is improved, and the culture cost is reduced. The culture quality is ensured.

The invention provides a schisandrin B-loaded nano-vesicle and functional hydrogel as well as a preparation method and application thereof, and belongs to the technical field of medical dressings. The preparation method comprises the following steps: blending human umbilical cord mesenchymal stem cells with schisandrin B, and carrying out gradient extrusion through a microfiltration membrane to obtain the schisandrin B-loaded nano-vesicles. The engineered stem cell-derived nanovesicles prepared by the invention can accelerate angiogenesis and promote liver cell proliferation, thereby promoting injury repair after hepatic resection. As a novel visceral wound dressing, the functional hydrogel provided by the invention not only has better biocompatibility, but also can adsorb blood cells and activate a blood coagulation cascade system so as to achieve a hemostatic effect.