10 results about "Filtration diaphragm" patented technology

The utility model discloses a low energy consumption water purification device. The low energy consumption water purification device includes the filter mechanism for filtering, the desalination mechanism for desalination and the disinfection mechanism for disinfection, the desalination mechanism includes main desalination pipe, vice desalination pipe, diaphragm pump, nanofiltration membrane component, membrane capacitance deionization device and valve component for the water flow into membrane capacitance deionization device or vice desalination pipe of alternative control, the filter mechanism and disinfection mechanism are connected through main desalination pipe, diaphragm pump, nanofiltration membrane component and membrane capacitance deionization device are installed in sequence on main desalination pipe, vice desalination pipe is provided with on main desalination pipe in parallel with membrane capacitance deionization device. The low energy consumption water purification device of the utility model can select desalination path according to TSD, avoids unnecessary equipment starting, and saves energy consumption.

This utility model provides an ultrafiltration centrifuge tube, including an inner tube and an outer tube. The outer edge of the inner tube's opening overlaps the inner edge of the outer tube's opening. The gap between the outer wall of the inner tube and the inner wall of the outer tube forms a filtration cavity. The outer tube's port is provided with a cap. The upper end of the inner tube has a liquid storage cavity, and the lower end has a filtration cavity. A wedge-shaped block is provided in the filtration cavity. The inner tube has a first filtration surface and a second filtration surface arranged opposite each other on both sides of the filtration cavity. Both the first and second filtration surfaces are provided with filter membranes. The wedge-shaped block is located between the first and second filtration surfaces, dividing the filtration cavity into the first and second filtration cavities. The filter membrane is an ultrafiltration membrane, and the wedge-shaped block is wedge-shaped.

This invention provides a biomimetic microfluidic device for simulating the three-dimensional structure of alveoli. The fabrication method includes: preparing an inverse opal membrane; bonding the inverse opal membrane to the bottom of a transwell chamber structure (with the bottom filter membrane removed); bonding the top of the transwell chamber to a perforated plate cover; inserting one end of a gas delivery tube through the perforation in the perforated plate cover into the transwell chamber; and connecting the other end to an injection pump; culturing alveolar epithelial cells and vascular endothelial cells on the front and back sides of the inverse opal membrane, respectively, to obtain the biomimetic microfluidic device. The biomimetic microfluidic device provided by this invention can achieve three-dimensional stretching under the action of a microfluidic injection pump, simulating the periodic stretch-recovery process of human lung respiratory movement in vitro. The fabrication method is simple and reliable.

This invention discloses a highly efficient method for separating primary cells from cochlear tissue based on physical filtration. The method includes: S1: tissue pretreatment and viability preservation; S2: controlled mechanical dissociation; S3: automatic cell-impurity separation; b. differential centrifugation using a double-layer filtration centrifuge device; S4: impurity and cell removal and cell purification; S5: primary cell seeding and optimized culture. This separation method utilizes the natural differences in volume and mass between cells and bone fragments through a double-layer filtration centrifuge device and differential centrifugation filtration, simultaneously performing centrifugation sedimentation and filtration separation. Compared to traditional manual filtration or density gradient centrifugation, this device is simple to operate, has a fast separation speed, fundamentally avoids filter membrane clogging, and significantly improves separation efficiency, enabling the gentle and efficient separation of highly active primary cells from cochlear tissue.

The application relates to a laboratory integrated filtering device, characterized in that the device comprises a supporting base, a water storage cup body, a filtering cup body, a flow guide connecting pipe assembly, a filter membrane and a connecting sleeve, five flow guide connecting pipe assemblies are arranged on the top of the supporting base, the five flow guide connecting pipe assemblies are vertically fixed on the top of the supporting base and arranged in a linear shape, a guide groove is arranged in the bottom of the supporting base below the five flow guide connecting pipe assemblies, the upper end of the connecting sleeve is connected in the guide groove through a first spring, the lower end of the flow guide connecting pipe assembly enters the guide groove below the flow guide connecting pipe assembly and extends into the upper end of the connecting sleeve, and the lower end of the connecting sleeve is sleeved on the upper end of the water storage cup body under the action of the first spring. The five flow guide connecting pipe assemblies are arranged on the supporting base, and the design can complete the filtration of five samples at the same time, so that the work efficiency is greatly improved.

This invention discloses a method for preparing regulatory T cell-derived functionalized reconstructed vesicles, comprising the following steps: obtaining and expanding high-purity human Treg cells; resuspending the Treg cells in buffer solution, adjusting the density, and pre-treating with ultrasound; then, using a liposome extruder driven by a constant-speed injection pump, sequentially passing the Treg cell suspension through polycarbonate filter membranes with pore sizes of 10 μm, 5 μm, 1 μm, and 0.4 μm at different pressures for stepwise reconstruction; subsequently, enriching and purifying the reconstructed vesicles by high-speed centrifugation, graded filtration, and ultracentrifugation. The Treg-RVs prepared by this invention have uniform particle size, high concentration, and completely retain the core immunomodulatory functions of parental Treg cells, effectively inhibiting T cell proliferation.

This utility model discloses an automatic cell counting device, relating to the field of biomedical engineering technology. It includes a base with a metering box fixedly connected to the left side of the top of the base. The automatic cell counting device is equipped with a polycarbonate filter membrane, a superhydrophilic coating, and a Venturi tube. In use, a mucus sample is placed on the polycarbonate filter membrane, first contacting the superhydrophilic coating to prevent the adsorption of mucin in the mucus. The sample then flows downwards into the polycarbonate filter membrane, where the mucus is filtered to obtain a single-cell suspension. This suspension flows downwards into the Venturi tube, creating a micro-negative pressure environment as the cells pass through, preventing mechanical shearing forces from damaging the cells. The filtered cells then fall into a collection tray, which is placed on a detection pool. The sample is then imaged using a microscope imaging head, and the images are automatically identified and counted using image processing algorithms. This addresses the problem of devices being unable to handle viscous samples.

The application discloses a multi-channel double-layer membrane anti-blood cell interference electrochemical test sheet and application thereof, and belongs to the technical field of electrochemical test sheets, and comprises a base material, a sensing electrode, a top sealing layer, and a double-layer membrane structure composed of an enzyme reaction membrane layer and a blood cell filtration membrane layer, wherein the double-layer membrane structure is located above the sensing electrode. The application realizes a completely independent multi-channel microfluidic system through physical structure separation, and sets a three-dimensional protruding structure at the blood inlet of each microfluidic channel, thereby significantly improving blood absorption starting speed and sample entering efficiency, and solving the problems of unstable blood absorption efficiency and difficult-to-avoid cross interference in the existing multi-channel design in the structural aspect. The application adopts a double-layer membrane structure, constructs a physical filter membrane layer with a stable pore size structure through PU / PVA blending, and stacks the physical filter membrane layer on a glucose dehydrogenase reaction layer, so that red blood cells are blocked from entering a detection area from the source, and the influence of HCT on a signal is reduced.

The application discloses a kind of carbon dots based on paclitaxel, its preparation method, application and brain glioma treatment drug, the preparation method of the carbon dots includes the following steps: S1, paclitaxel is dissolved in ultrapure water, then ethanol solution is mixed, ultrasonic;S2, the mixed solution obtained in step S1 is transferred to reaction kettle, and heated reaction;S3, after reaction, the product is filtered with filter membrane, the filtrate is dialyzed, the solution obtained by dialysis is freeze-dried, to obtain the carbon dots.The carbon dots synthesized by one-pot hydrothermal method using paclitaxel have the advantages of good water solubility, low toxicity, strong penetration capacity, etc., have the ability of efficiently penetrating blood-brain barrier and killing brain glioma cells, can significantly inhibit the proliferation and migration of brain glioma cells, and the application can provide a feasible new strategy for developing new brain glioma treatment agents.