69 results about "Filtration diaphragm" patented technology

The invention discloses a rare cell filter and application thereof. The filter comprises a filter pipe, wherein an opening is formed at one end of the filter pipe; an elastic sealing head sliding along the inner wall of the filter pipe is arranged inside the filter pipe; a syringe sliding along the inner wall of the filter pipe is arranged above the elastic sealing head in a matching manner; the syringe comprises a bobbin with openings at two ends; a barrel cover is arranged above one end of the bobbin; a filter support ring is arranged at the opening of the bobbin below the barrel cover; a micro-hole array filter membrane with covered opening is arranged on the filter support ring; the bobbin, the elastic sealing head and the filter support ring form a recovery pool; the filter membrane is formed by a conical micro-hole and dual layers of micro-hole arrays with different calibers; a sample tube is arranged inside the recovery pool; one end of the sample tube passes through the filter support ring and extends into a filter cavity; the other end of the sample tube passes through the elastic sealing head and extends into a sealed cavity. By adopting the rare cell filter, the problems of poor selectivity and sensitivity for capturing rare cells are solved, and the rare cell filter is applicable to efficient capture, cultivation and analysis of circulating tumor cells and fetal circulation cells in human peripheral blood.

The present invention discloses a blood type detection method based on a membrane structure. According to the method, a filtration membrane allowing a single red blood cell to pass through is selected as a core material, blood type antibodies are bound to the filtration membrane, a water absorption material is surrounding the periphery of the filtration membrane, red blood cells with a certain concentration are added on the filtration membrane surface in a dropwise manner during detecting, rinsing is performed with a rinsing liquid, the red blood cells with no antigen-antibody binding exude along with the rinsing liquid, the red blood cells with the antigen-antibody binding are remained on the membrane surface so as to form the visible red clumps, and an operator can judge the result according to whether the red blood cells form the visible red clumps on the filtration membrane so as to determine the blood type of red blood cells. The method of the present invention has beneficial effects of short detection time and objective result.

The invention relates to a biological agent for treating habitual abortion in the medicine technological field, which comprises the following steps: extracting peripheral blood of healthy adult men; diluting the blood to 1. 5 times through glass fibers from the ball and Hanks liquid to separate lymphocyte to collect single core cells; washing by Hanks liquid to culture and flow and count with absolute culture liquid of RPMI-1640; adjusting the cell concentration to 2X106 / ml to add phaescolosaxin to culture at 37 Deg. C for 72hours; collecting supernatant to produce T cells through centrifuging of centrifugal ultrafiltration and cane sugar density gradient centrifugation; filtering by sterile filter membrane to be split charging to produce completed product at 80 Deg. C below zero. The invention evokes immune tolerance of body effectively and overcomes the more faults of evoking immune tolerance through cell adoptive immunotherapy to make easier clinical application, which can be produced in a large industrialization scale to get more stabilizing mass control and higher controllability for treating the habitual abortion with good curative effect.

The invention belongs to the technical field of electrochemical energy storage, and discloses preparation and application of a modified ultrathin cellulose diaphragm. According to the invention, cellulose or functional material modified cellulose is directly sucked and filtered to a filter membrane substrate by adopting a vacuum suction filtration method, and the filter membrane substrate is peeled off after drying, so that the modified ultrathin cellulose diaphragm is constructed. The modified ultrathin cellulose diaphragm has the effects of high puncture strength, promotion of homogenization of a zinc negative electrode surface electric field and an ion field during zinc ion deposition, optimization of zinc deposition crystal face orientation, reduction of a nucleation barrier and the like, so that the effect of inhibiting growth of zinc dendrites is achieved. Therefore, the water-based zinc-based energy storage system with high stability and long cycle life is realized, and the method has important significance in promoting the practical application of the water-based zinc-based energy storage system.

In one embodiment according to the invention, there is provided a filter device for liquid filtration, the filter device being in fluid communication with a plurality of liquid pathways, at least one pathway of the plurality of liquid pathways having a smallest equivalent fluid flow diameter of the plurality of liquid pathways. The device comprises a filter membrane (108) and a filter core (109) comprising at least one cross hole (110) in fluid communication with the filter membrane, each cross hole having an equivalent fluid flow, diameter. The sum of the equivalent fluid flow diameters of the at least one cross holes does not exceed the smallest equivalent fluid flow diameter of the plurality of liquid pathways.

The invention relates to clinical single-tube fluorine 18 multifunctional module equipment and a radiopharmaceutical synthesis process. The clinical single-tube fluorine 18 multifunctional module equipment comprises a fluorine-18 ioncapturing and releasingmodule, a high performance liquid chromatography column purification module and a product collection module, wherein the high performance liquidchromatography column purification module adopts liquid for intravenous injection as an HPLC mobile phase; a QMA column of the fluorine-18 ion capturing and releasing module captures fluorine-18 ionsgenerated from an accelerator, and the leacheate of the QMA column and a prodrug respectively enter a reaction tube for mixing with the HPLC mobile phase; the reaction tube is connected with an HPLCseparation column of the high performance liquid chromatography column purification module; the HPLC separation column is connected with the product collection module; and a product having been subjected to online dilution and sterile filtration membrane treatment can be used for intravenous injection. By adopting the equipment and the process provided by the invention, solvent conversion used bya common module can be avoided, a target compound can be correctly collected, the pH value and the radioactive concentration of the product can be adjusted on line, and the process is suitable for full-automatic preparation of clinically common positron emission drugs under a GMP condition.

The invention provides a battery diaphragm coating head cleaning wastewater treatment device and treatment method. The treatment device comprises a gravity settling tank, a coagulation and precipitation tank, an AO membrane biological treatment unit and a rolling type nano-filtration membrane unit which are sequentially communicated through a connecting pipeline; the treatment method comprises thestep of obtaining clear water at an outlet of the rolling type nano-filtration membrane unit after battery diaphragm coating head cleaning wastewater sequentially flows through the gravity settling tank, the coagulation and precipitation tank, the AO membrane biological treatment unit and the rolling type nano-filtration membrane unit. According to the technical scheme of the invention, the wastewater is deeply treated by adopting sedimentation, coagulation and sedimentation, AO membrane biological reaction and rolling type nano-filtration system and other various treatment processes, so thatthe obtained clear water is better in water quality, the chemical oxygen demand (COD) is reduced, and nitrides in the wastewater are reduced, therefore, the battery diaphragm coating head cleaning wastewater is recycled.

The invention discloses a portable water purifying device which comprises a casing, wherein a hand-operated water pump and a water filter device are mounted in the casing; the hand-operated water pump comprises a rocking handle, a reduction box and a pump body which are sequentially connected, the pump body is provided with a first working cavity and a second working cavity which are mutually spaced and provided with water inlets and water outlets, and a water inlet of the first working cavity is connected with a water pumping pipe extending out of the casing; and the water filter device comprises an ultra-filtration diaphragm filter, an RO (reverse osmosis) diaphragm filter and a water storage box, the water outlet of the first working cavity, a water inlet and a water outlet of the ultra-filtration diaphragm filter, a water inlet and a water outlet of the water storage box, the water inlet and the water outlet of the second working cavity, as well as a water inlet and a water outlet of the RO diaphragm filter are sequentially connected in series and communicated, and the water outlet of the RO diaphragm filter is connected with a water outlet pipe extending out of the casing. The portable water purifying device can pump water at 10-15 m depth underground, the lift can reach a height of 150-200 m, and various types of water can be deeply filtered and treated to meet the standard of purified water for drinking.

The present invention discloses a circulating tumor cell isolation and enrichment device based on a closed loop. The circulating tumor cell isolation and enrichment device based on the closed loop includes a top plate; two ends of the bottom of the top plate are respectively fixedly connected with a left bracket and a right bracket located at the right side of the left bracket; the left side of the right bracket is fixedly provided with a device main body which is of a hollow structure; the bottom of the device main body is provided with a sample outlet where a liquid collecting bottle is disposed, and a filter membrane is further disposed between a top bottleneck of the liquid collecting bottle and the sample outlet; the inside of the device main body is movably provided with an oscillation plate; and the top of the oscillation plate is fixedly provided with a lifting bar and a toothed bar located at the right side of the lifting bar. In the circulating tumor cell isolation and enrichment device based on the closed loop, the activity of a sample liquid in the device main body may be accelerated, the disadvantage that an original liquid is relatively still and relatively low in flowability and activity is changed, and the isolation of the sample liquid and the filter membrane in a cell enrichment device may be further accelerated, so that the tumor cell isolation and enrichmentefficiencies are greatly increased, and the use demands of people are met.

A whole blood filtration device is provided with a filter membrane separating a feeding volume and a clean side of the filter membrane from each other. The feeding volume communicates with a first feeding side opening and with a second feeding side opening. The filter membrane has pores with a pore size that ensures permeability of the filter membrane to blood plasma / serum and that retains blood cells. The first feeding side opening can be coupled to a first blood pump for feeding blood from the first feeding side opening into the feeding volume so that blood plasma / serum permeates the filter membrane and blood cells, retained by the filter membrane, exit from the feeding volume through the second feeding side opening.

The invention relates to a serialized perfusion type microfluidic device. The serialized perfusion type microfluidic device comprises a plate-shaped perfusion side body, a plate-shaped intermediate layer body and a plate-shaped cell side body which are arranged from top to bottom in sequence; the perfusion side body is provided with a perfusion side flow passage and a perfusion side inlet, the intermediate layer body is provided with three membrane windows or above, and the membrane windows are filled with microfiltration membranes; the cell side body is separately provided with a cell side inlet, a cell side flow passage and a cell side outlet which are sequentially connected; the perfusion side inlet and the cell side outlet are correspondingly positioned at the same side of the device from top to bottom, and the cell side inlet is positioned at the other side of the device; when the device works, perfusate is injected into the perfusion side inlet, and cell suspension is injected into the cell side inlet; the perfusate flows into the cell side flow passage in an one-way mode through the membrane windows, the perfusate is mixed with the cell suspension, and the mixture is discharged from the cell side outlet. The device can avoid an accumulation effect of intracellular and extracellular osmotic pressure difference in continuous type treatment systems, including a diffusion method, a dialysis method, etc., thereby better adapting to high-throughput treatment requirements.

The invention relates to filtration system (20), comprising a tank (40) filled at least partly with water to be filtered, and at least one filtration module (30), the at least one filtration module (30) comprising at least one filter membrane (10) for filtering the water comprising a substrate (12) which is penetrated by at least one capillary (16), and at least one filtrate pipe (32) for drawing filtered water out of the tank (40), whereat the at least one filtration module (30) is arranged in the tank (40) such that the at least one filter membrane (10) is submerged at least partly in the water to be filtered. The at least one filtration module (30) is designed and arranged such that water to be filtered flows into the at least one capillary (16) and from the at least one capillary (16) through the substrate (12) into the filtrate pipe (32). The invention also relates to a method for filtering water by means of a filtration system (20) according to the invention, whereat the water to be filtered is drawn into the at least one capillary (16) and from the at least one capillary (16) through the substrate (12) into the filtrate pipe (32) and from the filtrate pipe (32) out of the tank (40).

The invention relates to the technical field of medical instruments and particularly relates to a cell transfer device. The device comprises a suction head, a three-axis mechanical arm, a main machine, a suction nozzle head and a positive and negative pressure generating device, wherein the suction head is of a hollow structure with openings in the two ends, an annular protrusion is arranged on an opening in a lower end of the suction head, and a filter film is welded to the protrusion; the main machine is arranged on the three-axis mechanical arm, one end of the suction nozzle head is connected with the main machine, the other end of the suction nozzle head is connected with an upper end opening of the suction head, and a through hole is formed in the suction nozzle head; the positive and negative pressure generating device is arranged in the main machine, the positive and negative pressure generating device is communicated with an inner cavity defined by the side wall of the suction head through the through hole, and the positive and negative pressure generating device is used for separating cells from the mixed liquid through the suction head and absorbing the cells on the filter film or blowing the cells from the filter film away from the filter film; and the invention further provides a cell transfer and pretreatment method adopting the cell transfer device. According to the method, cell pretreatment and cell transfer can be carried out at the same time, the operation process is simplified, centrifugal separation treatment is avoided, and staining and slide-making efficiency is effectively improved.

The invention discloses a device for collecting aerosol in a near space. The device comprises a gas inlet pipe, a gas flowmeter, a molecular pump and a diaphragm pump which are communicated in sequence. A gas inlet is formed in one end, far away from the gas flow meter, of the gas inlet pipe; a gas outlet is formed in one end, far away from the molecular pump, of the diaphragm pump; the flow meter, the molecular pump, the diaphragm pump and the battery are electrically connected with an electric control system respectively; a filter membrane and a supporting net are sequentially arranged in the gas inlet pipe in the axial direction, and the supporting net is closer to the gas flow meter than the filter membrane. According to the device for collecting aerosol in a near space, effective collection of the aerosol in the near space is achieved, and systematic research on the aerosol in the near space is facilitated.

The present invention relates to a method for filtering blood to produce plasma or serum, the method comprising the steps of: a. providing a blood filter comprising a filter membrane having opposing first and second sides (6) and a receiving chamber (2) defining a hollow space; and b. injecting a blood sample (1) into said receiving chamber (2), wherein said hollow space has a larger volume than said blood sample (1) The volume is 3 to 20 times larger, thereby increasing the gas pressure in the receiving chamber, so that the blood sample is filtered by the filter membrane (6), and the plasma or serum contained in the blood sample is forced to through the filter. The invention also relates to a method for filtering blood to produce plasma or serum, said method comprising the steps of: a. providing a blood filter comprising a filter having opposite first and second sides; Membrane (6) and a receiving chamber (2'') with a first volume; b. inject blood sample (1) and gas (2') into the syringe, said blood sample (1) occupying the second volume of said syringe volume and the gas (2') occupies a third volume in the syringe; c. connect the syringe to the blood filter so that they are in fluid communication with each other; and d. increase the pressure in the syringe until The blood sample (1) is received by the receiving chamber (2), so that the blood sample (1) is filtered by the filter membrane (6); wherein, the plasma contained in the blood sample or The serum is forced to pass through the filter membrane (6); the sum of the first volume and the third volume is 3 to 20 times larger than the second volume. The present invention also relates to a filter membrane (6), the The filter membrane has opposite first and second sides; the receiving chamber (2) defines a hollow space for receiving the blood sample to be filtered, the receiving chamber has at least one opening, the at least one opening is covered by the Covered by the filter membrane (6), wherein the first side faces the receiving chamber (2), and the volume of the hollow space of the receiving chamber is 3 to 20 times larger than the volume of the blood sample to be filtered; The sampling chamber (10) is arranged on the second side of the filter membrane (6).

The invention discloses a safe water surface treatment device for oil leakage. The safe water surface treatment device comprises a box body, a water inlet pipe, a pressing plate, a drain pipe and an igniter, wherein the water inlet pipe is arranged at the bottom end of the box body, a pump and a flow meter are arranged on the water inlet pipe, grids are arranged in the water inlet pipe, the igniter is arranged on the inner wall of the box body, an air bag is arranged at the bottom end of the box body, an oxygen filling tank is mounted on the outer wall of the box body, a breather pipe is arranged on the oxygen filling tank, the breather pipe is provided with two outlet ends, one outlet end of the breather pipe is connected with an air valve of the air bag, the other outlet end of the breather pipe is provided with a spray row, the spray row is arranged in the box body, the side wall of the box body is communicated with an air collector through a suction pipe, a gas one-way valve is mounted on the suction pipe, a water filtration diaphragm plate is arranged in the box body, a telescopic rod is arranged on one side of the water filtration diaphragm plate, and the pressing plate is arranged at the bottom end of the telescopic rod. The safe water surface treatment device reduces oil contamination, facilitates environmental protection, and is simple in structure, convenient to use,and beneficial to popularization.

The invention relates to the technical field of engineered exosomes, and discloses a method for increasing the content of exosomes released by cells, which comprises the following steps: 1) treating a cancer cell line; 2) filtering the treated cancer cell supernatant with a 0.45 [mu] m filter membrane, and collecting the filtered liquid; (3) centrifuging 120000g of the solution for 90 minutes, and extracting the exosome; 4) collecting the exosome; the cancer cell line treatment comprises the following steps: 1) replacing a fresh culture medium for cancer cells; 2) irradiating cancer cells with UVB for 1 hour, and paying attention to the state of liver cancer cells at any moment; 3) culturing the cancer cells in an incubator for 2 hours; 4) placing the cancer cells in an anoxic device for 1 h; and 5) collecting the supernatant of the cancer cells after 24 hours. The exosome release amount can be increased under the condition that cell genetic materials are not changed, the method is suitable for various cell lines, compared with an ultrasonic method, an LED lamp irradiation method, a special culture medium treatment method and other methods, the method is simpler, and the exosome release amount of the various cell lines can be better increased.

The invention discloses a full-automatic sheep blood PBMC (peripheral blood mononuclear cell) separation system and method. The full-automatic sheep blood PBMC separation system comprises an input module, a separation module, an output module, a pipeline module and a power module, wherein the pipeline module comprises an input pipe, a peristaltic pipe and an output pipe; the head end of the input pipe is provided with a filter membrane structure, and the tail end of the input pipe and the head end of the output pipe both communicate with the head end of the peristaltic pipe; and the input end and the output end of the separation module both communicate with the tail end of the peristaltic pipe, and the power module is arranged on the peristaltic pipe and used for driving liquid in the peristaltic pipe to flow. The separation process is full-automatic, the consistency of the production environment, the raw materials and the production process can ensure that each batch of cells are prepared under the same standard condition, and the stability and uniformity of the cell quality and the repeatability of experimental results are ensured. The problem that the peripheral blood mononuclear cells need to be extracted and prepared on a large scale in scientific research and new drug research and development animal experiments is solved, traditional manual operation is changed into automatic operation through intelligent equipment, efficiency is improved, and time of scientific research personnel is saved.

The invention relates to a cell filter. The cell filter comprises a blood aspiration device, a blood filtration device and a blood recycling device; the blood recycling device comprises a waste liquidbottle and a vacuum pump; the vacuum pump is communicated with the waste liquid bottle; a blood aspiration needle is arranged in the blood aspiration device; the blood filtration device is correspondingly provided with an upper sealing cavity, a movable support plate and a lower sealing cavity in sequence from top to bottom; the lower sealing cavity is fixed on a lifting system; blood reaches a blood flow-in needle of the blood filtration device through the blood aspiration needle of the blood aspiration device, and then, reaches the waste liquid bottle through a filter film and a blood recycling pipeline; and the calibre of the blood aspiration needle is less than that of the blood flow-in needle. According to the cell filter in the invention, the blood aspiration needle is arranged at the input end; the blood flow-in needle is arranged at the output end; furthermore, the calibre of the aspiration needle is less than that of the flow-in needle; therefore, blood can be filtered by adoption of ultra-low negative pressure; the low pressure has little effect on cell damage; a new membrane pore structure is adopted; holes are same in size and arrangement form; and pollution of white blood cells on membrane pores is avoided.

The invention relates to the technical field of medical treatment, in particular to a pretreatment tube and a pretreatment method for a liquid-based cell sample.The pretreatment pipe comprises a pipe body; a pair of slots which are formed in the inner wall of the pipe body; an inserting plate, the width of the inserting plate is matched with the spacing distance of the pair of inserting grooves, and the inserting plate is used for being embedded into the pair of inserting grooves so as to divide the pipe body into a first cavity and a second cavity which are closed with each other; a filter membrane is arranged on the insertion plate and is provided with a plurality of filter holes, and the size of the filter holes is smaller than that of the cells to be treated. According to the technical scheme, the pretreatment tube has the beneficial effects that the insertion plate is arranged in the tube body of the pretreatment tube to divide the tube body into the first cavity and the second cavity, and the insertion plate is provided with the filter membrane with the filter holes, so that the tube body is not only used for storing cell samples, but also can realize other reaction functions such as dilution, enrichment and dyeing in the tube body.

The invention discloses a filtering centrifuging tube type lymphocyte culture tube and a culture method. The culture tube comprises a centrifuging tube, a filter and a tube cover, wherein an upper part of the centrifuging tube is a cylindrical tube, the filter is a detachable cylindrical tube, the diameter of the bottom of the filter is smaller than that of a tube body of the filter, the bottom ofthe filter is a meshed bottom face, a filter membrane and a pressing ring are sequentially arranged above the bottom of the filter, an external hanging edge is arranged at a tube orifice of the filter, a circular hole is formed in the center of the tube cover, and a rubber plug is arranged inside the tube cover. According to the lymphocyte culture method, lymphocytes are cultured by using the culture tube. The culture tube is simple in structure and ingenious in design and integrates the functions of culturing, centrifuging and cell filtering; and by using the innovative centrifugal culture tube with the filter, convenience is provided for human peripheral blood chromosome karyotype analysis, operating steps are greatly simplified, the experimental time is shortened, and meanwhile, a stable experimental result can be obtained.

The invention provides a cross-flow filtration system for enriching circulating tumor cells. The system comprises: a tumor cell sap-to-be-filtered volumetric flask (1); three peristaltic pumps which are a first peristaltic pump (2-1), a second peristaltic pump (2-2) and a third peristaltic pump (2-3) respectively; a centrifugal tube (3); a filtering chip (7); and a cross-flow filtered tumor cell sap volumetric flask (5). A process represented by figure 1 is a new method provided on the basis of filter membrane filtration and a micro-fluidic fluid principle. The cross-flow filtration is characterized in that the flow direction of a water phase is parallel to the surface of the filter membrane to form a large pressure on the surface of the filter membrane, so cells enter filter holes, and are filtered and screened, and the activity of the cells is not affected after the filtration in order to achieve the efficient enrichment effect. The cross-flow filtration system for enriching circulating tumor cells reaches the efficient enrichment effect. The system has the advantages of simplicity in sample treatment, high recovery rate of the sample, simplicity in operation, convenience in use,facilitation of promotion, good clinic application prospect and great social benefit.

The invention discloses a microfluidic chip, a whole blood hemocyte separation system based on the chip, a preparation method of the system and a method for separating whole blood hemocytes by using the system, is used for separating the hemocytes, can overcome the defects of low separation efficiency and insufficient miniaturization and lightweight in the prior art, and meets on-site real-time blood sample separation requirements. The chip is prepared by successively bonding a substrate layer containing a main flow channel structure with a microporous filter membrane layer and a flexible polymer layer containing an upper-layer flow channel structure, and a micro-channel system is formed in the chip; the micro-channel system includes a micro-pump area, a circulation area, a first channel, a second channel, a third channel, and a filtering area formed by the main flow channel structure, the microporous filter membrane layer and the upper-layer flow channel structure.

Provided is a red blood cell filtering apparatus comprising: a filtering film having multiple through holes; and a shell having a first opening on an upper portion of one lateral part of the shell, and a second opening on a lower portion of the other lateral part of the shell; the filtering film is accommodated in the shell. Also provided is a red blood cell filtering system comprising the red blood cell filtering apparatus, a first blood collector and a second blood collector; wherein the first blood collector has a first connecting opening, and the first connecting opening is connected with the first opening through the first connecting apparatus; the second blood collector has a second connecting opening, and the second connecting opening is connected with the second opening through the second connecting apparatus.

The invention provides a method for extracting and purifying drug-coated cell vesicles from a cell suspension, comprising: 1) filtering the cell suspension to collect the filtrate; 2) centrifuging the filtrate collected in step 1) to collect the precipitate, Obtaining drug-encapsulated cell vesicles; wherein the filtration is filtering the cell suspension through a filter membrane or filter element with a pore size of 5 μm-10 μm; the centrifugation is high-speed centrifugation with a centrifugal force of 100-100000 g; the The drug-encapsulated cell vesicles are microparticles containing tumor therapeutic drugs released by apoptotic cells, and the cell suspension is a mixed liquid containing the cells, cell fragments and cell vesicles. The process of the method of the present invention is relatively simple, the loss of the instrument is small, there is no pollution, the yield of cell vesicles is high, and it is beneficial to realize industrial production, and the cell vesicles wrapped with drugs obtained by the method have higher drug-containing quantity and better therapeutic effect.

Experimental microfiltration membrane equipment for concentrating a Dunaliella salina liquid is used for concentrating a Dunaliella salina liquid. The equipment comprises a microfiltration membrane, awater inlet pipe, a first ball valve, a pressure vacuum gauge, a diaphragm booster pump, a second ball valve, a third ball valve, a flowmeter and a water outlet pipe connected in sequence; the diaphragm booster pump is powered and the equipment is controlled to start and stop through the arrangement of a time relay and a power adapter. The time relay, the pressure vacuum gauge, the diaphragm booster pump, the power adapter and the second ball valve are fixed inside a distribution box, and the first ball valve, the third ball valve and the flowmeter are respectively fixed on different outer side walls of the distribution box. The invention has the beneficial effects as follows: the structure is simple; by setting the concentration time and controlling pre-pumping pressure of the diaphragmbooster pump, the pre-pumping pressure of the diaphragm booster pump is controlled to be 0.01 MPa and below; and after membrane filtration for concentration, the Dunaliella salina activity is kept good, and after late culture, various physiological indexes are not much different from physiological indexes of the original control group, and the concentration effect is ideal.

The invention discloses an integrated purification column integrating extraction, purification and filtration and application thereof.The integrated purification column comprises a cavity column body, a first sieve plate, a second sieve plate and a third sieve plate are sequentially arranged in the cavity column body in the fluid flowing direction, and the space between the second sieve plate and the third sieve plate is filled with purification filler; and a filter membrane is arranged below the third sieve plate. According to the integrated purification column disclosed by the embodiment of the invention, a sample does not need to be centrifuged, filtered and repeatedly transferred after being extracted, the sample directly enters the integrated purification column to be purified through the sieve plate under the action of gravity, and the sample can be directly detected after being filtered, so that the pretreatment of the sample is controlled within 30 minutes from 60 minutes; and the cost increase and environmental pollution caused by the use of consumables such as centrifugal tubes, gun heads, injectors and the like are reduced.

The invention discloses gas-liquid separation equipment facilitating gas concentration testing. The gas-liquid separation equipment comprises an upper casing, a lower casing and a gas-liquid filtration diaphragm which constitute a gas accommodating cavity, a transition cavity is formed between the lower casing and the gas-liquid filtration diaphragm, a liquid inlet pipe and a liquid outlet pipe which are communicated with the transition cavity are arranged on the side wall of the lower casing, a control assembly is arranged on the liquid inlet pipe, the control assembly comprises a pressure limiting assembly arranged on a pipeline between a liquid inlet and a liquid inlet as well as a pressure relief assembly arranged on a channel connecting the liquid outlet and a liquid drain port in the pressure limiting assembly. Spiral isolation plates wind around the outer side wall of the gas-liquid filtration diaphragm and divide the transition cavity to form spiral channels communicated withthe liquid inlet pipe and the liquid outlet pipe. The gas-liquid separation equipment can avoid damage to inner assemblies and is convenient to operate and high in separation efficiency when separating gas in a liquid, and thus, the detection efficiency of gas in the liquid can be improved.