41 results about "Filtration diaphragm" patented technology

The filtration element (1) includes a filtration membrane (3) enclosed within a housing (2). The element has openings for removing filtrate and concentrate. A filter (11) is disposed within the element (1) at an opening (6) in the housing (2) for introducing feed into the interior volume (20) of the filtration element (11). Alternatively, the filter (11) may be positioned within an end cap (7) associated with such opening. Pre-filtration is thus performed within the filtration element 1 itself, rather than as a separate upstream operation. The presence of the filter (11) does not significantly increase the pressure drop across the filtration module. Flocculated particles and other debris that accumulate on the membrane can be easily removed by backwashing, despite the presence of the filter (11).

The utility model discloses a low energy consumption water purification device. The low energy consumption water purification device includes the filter mechanism for filtering, the desalination mechanism for desalination and the disinfection mechanism for disinfection, the desalination mechanism includes main desalination pipe, vice desalination pipe, diaphragm pump, nanofiltration membrane component, membrane capacitance deionization device and valve component for the water flow into membrane capacitance deionization device or vice desalination pipe of alternative control, the filter mechanism and disinfection mechanism are connected through main desalination pipe, diaphragm pump, nanofiltration membrane component and membrane capacitance deionization device are installed in sequence on main desalination pipe, vice desalination pipe is provided with on main desalination pipe in parallel with membrane capacitance deionization device. The low energy consumption water purification device of the utility model can select desalination path according to TSD, avoids unnecessary equipment starting, and saves energy consumption.

The utility model discloses an efficient separator for human seminal spot mixed spots, which relates to the technical field of separators and comprises a centrifugal tube and a detachable sleeve matched with the centrifugal tube, the caliber of the detachable sleeve is consistent with that of the centrifugal tube, and a sleeve cavity for containing biological material evidences is arranged in the detachable sleeve. A plastic filter plate is arranged in the middle of the sleeve cavity, a filter membrane filter layer is arranged at the bottom of the sleeve cavity, and a bottom drainage hole is formed in the bottom of the detachable sleeve. According to the utility model, a two-stage filtering mode of carrier removal filtering and sperm enrichment filtering is constructed in one sleeve, so that a biological material evidence carrier can be efficiently removed, water-soluble substances of human epithelial cells can be quickly filtered, sperm cells can be efficiently separated from related biological material evidences only by using a small amount of biological material evidences, and the operation is simple and convenient. The test speed and efficiency are greatly improved, difficult biological material evidence treatment is simplified, the experiment repeatability is good, the applicability is high, biological material evidence samples are saved, and the cost of related experiments is reduced.

This invention relates to the construction and application of an exosome purification device. The purification device utilizes the differences in size and charge between exosomes and other substances, combining the tangential flow of the sample within the device with the dual effects of membrane sieving and an electric field to remove contaminants and achieve high-purity exosome enrichment. The device includes a pre-filtration section and an exosome purification section. This device is simple to operate, can handle large sample volumes, has a short processing time, is less prone to membrane clogging by contaminants, and achieves high purity exosome separation with minimal structural damage. It can meet the separation and purification needs of exosomes from different sources and can be further applied to research on exosome proteomics, drug loading, activity, and biological functions.

The invention provides a cell product production instrument and a cell product production method using the same. The cell product production instrument comprises a whole blood sample bag; a magnetic separation device; the target cell processing module comprises a module main body, an accommodating cavity is formed in the module main body, a filter membrane is arranged in the accommodating cavity, the accommodating cavity is divided into a first space and a second space by the filter membrane, and a transfection reaction cavity capable of accommodating target cells is formed in the bottom of the first space; a heating module; a sample pumping assembly; the cleaning fluid pumping assembly is used for pumping the cleaning fluid in the cleaning fluid bag into the first space through the cleaning fluid inlet; the reagent pumping assembly is used for pumping a transfection reagent and / or a culture medium into the first space through the reagent inlet; and the cell product pumping assembly is used for conveying the transfected target cells in the first space into the finished blood bag through the cell product outlet. According to the invention, manual intervention and process breakpoints are thoroughly eliminated by realizing full-automatic internal circulation among the modules.

The present invention relates to the field of exosome extraction. Disclosed are a low-temperature exosome extraction device and a method for using same. The low-temperature exosome extraction device comprises an extraction stand and a bearing platform fixed on the extraction stand, and further comprises a plurality of filter cylinders mounted on the bearing platform at equal angular intervals, a collection cylinder circumferentially slidably mounted on the extraction stand, a vacuum tube connected to the collection cylinder, and liquid intake tubes fixed on the extraction stand and used for adding an auxiliary exosome extraction reagent into the filter cylinders, wherein a sliding block is slidably mounted on the side wall of the collection cylinder, a connecting ring for connecting and sealing the collection cylinder and a filter cylinder is fixed on the sliding block, and a steering support is fixedly connected to the collection cylinder; and the extraction stand is provided with a steering assembly for controlling the collection cylinder to rotate to the position under different filter cylinders. The low-temperature exosome extraction device simplifies the extraction structure, and, during multiple filtration for exosome extraction, agitates and disperses a cell fluid to be filtered, thereby preventing macromolecular exosomes from clogging a filter membrane, and improving the purity of extracted exosomes.

The invention discloses a large-scale high-quality plasmid extraction and purification process, which belongs to the technical field of molecular biology and mainly comprises the following steps: thallus cracking, solid-liquid separation and clarification, concentration and liquid exchange, plasmid purification and quality inspection and warehousing. Wherein the plasmid purification step comprises the following steps: adding an ammonium sulfate solid into a plasmid extraction concentrated solution, and then centrifuging. And centrifuging, recovering the centrifuged supernate, filtering by using a 3-micron filter membrane, then carrying out ultrafiltration concentration by using a 10-750KDa membrane bag, adding pure water, continuing to concentrate and change the liquid, and thus obtaining the purified plasmids. According to the method, plasmids are purified by adopting a one-step salting-out method, the plasmid purification step can be completed only in 1-2 h, compared with an existing column chromatography method, the purification time can be shortened, the purification cost can be reduced, and the method is suitable for obtaining different plasmids.

The application discloses a hemostasis valve assembly and a device for delivering a delivery system, and applies to the technical field of medical devices, and has the technical scheme as follows: a head end hemostasis part comprises a hollow circular table provided with a first cavity, and a first hemostasis channel is arranged on the head side of the hollow circular table; a blocking filter membrane is installed on the tail side of the hollow circular table and blocks the first cavity, and a second hemostasis channel is arranged on the blocking filter membrane; a middle end hemostasis part comprises a ring-shaped cylinder provided with a second cavity, and the head end of the ring-shaped cylinder is in abutment with the blocking filter membrane; a tail end hemostasis part is sleeved on the tail part of the ring-shaped cylinder and is used for blocking the second cavity, a third hemostasis channel is arranged on the tail end hemostasis part, the first hemostasis channel, the second hemostasis channel and the third hemostasis channel are arranged along the same axis, and when a penetrating object is withdrawn in a reverse order, the blocking filter membrane moves to the tail side direction to make the pressure of the second cavity greater than the pressure of the first cavity. The application can reduce the occurrence of blood leakage and achieve a good blood sealing effect.

This invention discloses a hemodialysis coagulation inhibition device based on porous modified materials, relating to the field of dialysis technology. It includes a housing with an upper cover and a lower cover fixedly installed at its upper and lower ends, respectively. An exchange chamber and an expansion chamber are interconnected within the housing. A porous modified material filter element is installed within the exchange chamber, with its bottom fixed to the housing and its top floating within the exchange chamber. An overflow pipe extending into the expansion chamber is fixedly installed on the exchange chamber. A Venturi drain pipe is fixedly installed at the bottom of the expansion chamber. This invention achieves mainstream bubble separation of the dialysate through a spiral guide plate, and the micro-negative pressure formed by the Venturi drain pipe and the suction pipe continuously removes gas from the expansion chamber. The porous filter membrane filaments oscillate, disturbing the flow field and peeling away microbubbles in dead corners of the membrane surface. These three elements work together to achieve deep degassing throughout the entire area. Furthermore, the reduction in bubbles reduces membrane surface deposits, further assisting in anticoagulation.

This utility model provides an ultrafiltration centrifuge tube, including an inner tube and an outer tube. The outer edge of the inner tube's opening overlaps the inner edge of the outer tube's opening. The gap between the outer wall of the inner tube and the inner wall of the outer tube forms a filtration cavity. The outer tube's port is provided with a cap. The upper end of the inner tube has a liquid storage cavity, and the lower end has a filtration cavity. A wedge-shaped block is provided in the filtration cavity. The inner tube has a first filtration surface and a second filtration surface arranged opposite each other on both sides of the filtration cavity. Both the first and second filtration surfaces are provided with filter membranes. The wedge-shaped block is located between the first and second filtration surfaces, dividing the filtration cavity into the first and second filtration cavities. The filter membrane is an ultrafiltration membrane, and the wedge-shaped block is wedge-shaped.

The invention discloses a method for capturing, identifying and culturing circulating tumor cells of sarcoma in vitro. The method comprises a step of filtering a peripheral blood sample of a sarcoma patient by using a flexible microfiltration membrane, and cells obtained by filtration can be directly subjected to multiplication culture on the filtration membrane. Immunomagnetic beads of a monoclonal antibody combination group coupled with the anti-human leukocyte surface antigen CD45 can be used for negative enrichment and then multiplication culture is carried out in a cell culture bottle, and the captured or cultured cells can be subjected to subtype identification, characterization and counting by adopting an iFISH technology. It is found for the first time that the flexible microfiltration membrane can be used for capturing and culturing the sarcoma circulating tumor cells, and the flexible microfiltration membrane has no toxic effect on the sarcoma circulating tumor cells. The invention not only provides an effective means for real-time monitoring of relapse and metastasis processes of sarcoma patients and chemotherapy drug resistance monitoring, but also can perform drug sensitivity experimental verification on CTC after multiplication culture, and provides a powerful weapon for accurate diagnosis and treatment of sarcoma.

The utility model relates to the technical field of biology, in particular to a U-shaped tube cell separation device which comprises a bottom plate, a top plate is arranged at the upper end of the bottom plate, a negative pressure extraction mechanism is arranged on one side, located on the top plate, of the bottom plate, a lifting mechanism is arranged on the other side of the bottom plate, a containing groove is formed in the top plate, and a U-shaped cell filter tube is arranged in the containing groove. And the bottom of the U-shaped cell filter tube is supported by a lifting mechanism. The U-shaped tube cell separation device has the advantages of being simple in structure, convenient to operate, high in cell separation efficiency, small in cell damage and the like, can be suitable for separation of various types of cells through different microfiltration membranes, and meets the requirements of the fields of biological experiments, cell therapy and the like.

The utility model discloses a colloidal gold test paper card for rapidly determining rice yeast acid in whole blood. The colloidal gold test paper card comprises a bottom plate as well as a glass cellulose membrane, a filtration binding area, a detection area and a water absorption pad which are sequentially arranged on the bottom plate from left to right, the filtration binding area adopts an antigen-antibody binding technology, red blood cells in whole blood can be retained on a filter membrane of a sample pad without moving, other substances in the whole blood can smoothly pass through, and the detection effect is good. According to the present invention, the kit can be combined with the rice yeast acid so as to eliminate the background interference, can be combined with the rice yeast acid, and can be detected through the detection region, such that the rice yeast acid in the whole blood can be accurately detected, the accuracy and the specificity of the rice yeast acid determination in the whole blood can be effectively improved, the application range and the efficiency of the trace whole blood sample in the detection can be expanded, and the redundant pretreatment operation is not required; the method is simple in operation and rapid in detection, and can obtain a result within 15 minutes only by taking 10 microliters of trace whole blood, and the lowest detection limit of 50 micrograms / L is reached.

The application provides a cell product production instrument and a cell product production method using the same. The cell product production instrument comprises: a whole blood sample bag; a magnetic sorting device; a target cell processing module comprising a module main body, a containing cavity is formed in the module main body, a filter membrane is arranged in the containing cavity, the filter membrane separates the containing cavity into a first space and a second space, and the bottom of the first space is provided with a transfection reaction cavity capable of containing target cells; a heating module; a sample pumping assembly; a cleaning liquid pumping assembly for pumping cleaning liquid in a cleaning liquid bag into the first space through a cleaning liquid inlet pump; a reagent pumping assembly for pumping transfection reagents and / or culture medium into the first space through a reagent inlet pump; and a cell product pumping assembly for conveying the target cells in the first space after transfection into a finished product blood bag through a cell product outlet. The application completely eliminates manual intervention and process breakpoints by realizing full-automatic internal circulation among the modules.

The invention belongs to the technical field of biological medicine, and particularly relates to gradient centrifugation leukocyte extraction equipment and method for inhibiting release of inflammatory factors, the equipment comprises a centrifugal cavity with a cover body, a placing cavity is arranged in the cavity body, the placing cavity is equipped with an illuminating lamp, an image sensor, a temperature sensor, a cooling assembly and a heating assembly, and the bottom is connected with a centrifugal driving assembly; the supporting frame at the top end of the placement cavity is matched with the supporting block, and the equipment stability and the sample treatment effect are improved by combining microfiltration membranes and other structures. According to the extraction method, the equipment is utilized, through the four steps of equipment pre-adjustment, sample preparation and placement, centrifugal process synchronous adjustment (including photo-biological adjustment, precise temperature control, gas signal molecule intervention and dynamic monitoring feedback) and leukocyte extraction, inflammatory factor release is effectively inhibited while gradient centrifugal separation is performed, the leukocyte extraction quality and activity are improved, and the leukocyte extraction efficiency is improved. The method is suitable for efficient extraction and subsequent research application of leukocytes in clinical blood samples.

Traditional hemofiltration devices can include a linear blood channel, a linear dialysate channel, or both. Additionally, the blood channel or dialysate channel can be modified so that one region has a recess in a channel wall and a filtration membrane can be positioned along the edge of the recess. However, due to fluid dynamics, some blood or dialysate could linger within the recess by moving slowly. Therefore, other fractions of the blood or dialysate would simply bypass the recess and filtration membrane by flowing through the center of the channel. This flow pattern would reduce filtration efficiency since some blood or dialysate remains far from the filtration membrane. However, positioning a flow diverter adjacent to the recess could force most or all of the blood or dialysate into the recess, thereby increasing filtration efficiency. In other words, the flow diverter would modify the fluid dynamics in a manner that would reduce the dead space in the blood channel or dialysate channel and increase filtration efficiency.

The invention relates to the technical field of cell culture, and particularly discloses a pancreas islet cell culture device in pancreas islet transplantation, which comprises an outer bracket, the outer bracket comprises a bottom plate, two shells are fixedly connected above the bottom plate, two moving assemblies are mounted in the shells, one side of each moving assembly is provided with a cam assembly, and the other side of each moving assembly is provided with a cam assembly; a cam assembly is installed on the top of the moving assembly, a motion sealing assembly is installed on one side of the cam assembly, a material receiving weight measuring assembly is installed at the bottom of the moving assembly, and a filter membrane assembly is movably connected to the top of the moving assembly. By utilizing the characteristic that gas and spray particles can pass through the fluidity of gas and the size of a gap in the film, the effect that a nutrient solution can be provided without opening a cover in the islet cell culture process is realized, the possibility that bacteria enter a culture dish in the culture process is greatly reduced, the environmental purity of islet cell culture is improved, and the culture cost is reduced. The culture quality is ensured.

The utility model discloses an infusion system air filter which comprises an air filtering flexible cavity, two purifying filter membranes are arranged in the air filtering flexible cavity, connecting cavities are installed at the two ends of the air filtering flexible cavity, and a connecting plug is installed at one end of each connecting cavity. After the connecting plug is connected with the connecting pipeline, the connecting ring moves to the inserting position of the connecting plug and the connecting pipeline by moving the two movable blocks, so that the sealing balloon abuts against the inserting position of the connecting plug and the connecting pipeline for sealing again, and the sealing effect is improved; the outer side wall of a second limiting convex block abuts against the outer side wall of a first limiting convex block, so that a movable block is self-limited, and the problems that the connecting part of an external pipeline and the device is prone to being disconnected, liquid medicine is lost, microorganisms can enter an infusion system through cracks, and the safety of the device is reduced are effectively solved.

The invention discloses a filtering device for removing blood cancer cells by combining an antibody modified filter membrane, the filtering device comprises a device main body, two ends of the device main body are respectively provided with a liquid inlet and a liquid outlet, the inside of the device main body is sequentially provided with a first-stage pre-filtering membrane, a second-stage targeting membrane and a third-stage fine filtering membrane from the liquid inlet to the liquid outlet, and the first-stage pre-filtering membrane, the second-stage targeting membrane and the third-stage fine filtering membrane are arranged in the device main body. A liquid homogenizing and shunting part is arranged between the primary pre-filtration membrane and the liquid outlet, the secondary targeting membrane comprises at least one antibody modification plate, axially through micropores are uniformly distributed in the antibody modification plate, and a surface covalent coupling EpCAM antibody, a CK antibody and a PD-L antibody are arranged on the inner side wall of each micropore. The problem that an existing single filtering technology cannot meet the requirement for blood safety of autologous blood transfusion in a tumor patient operation can be solved.

The utility model discloses a microclot filter which comprises a shell assembly, a filter element, a filter element, a filter element, a filter element, a filter element, a filter element, a filter element, a filter element, a filter element and a filter element, and is characterized in that the shell assembly is provided with an upper shell and a lower shell; the filter material assembly is provided with a filter membrane which is continuously folded, the filter membrane is peripherally and thermally sealed in the upper shell or the lower shell to form sealed connection, and the filter cavity is divided into a pre-filter cavity and a post-filter cavity. Through the design of the filter membrane which is continuously folded, the effective filtering area is increased to more than 40 times of that of a traditional structure, large-volume blood can be treated, and blockage is not prone to occurring; by means of the sealing connection structure, the bypass leakage risk is eliminated, 20-160-micrometer microclots are accurately intercepted, and especially the filtering efficiency of 40-60-micrometer medium-sized clots is remarkably improved. In addition, the gradient filtering composite membrane is adopted, the single-layer load is reduced in the whole process, the filtering efficiency is improved, and meanwhile the service life of the filtering membrane is prolonged by more than two times.

This invention provides a biomimetic microfluidic device for simulating the three-dimensional structure of alveoli. The fabrication method includes: preparing an inverse opal membrane; bonding the inverse opal membrane to the bottom of a transwell chamber structure (with the bottom filter membrane removed); bonding the top of the transwell chamber to a perforated plate cover; inserting one end of a gas delivery tube through the perforation in the perforated plate cover into the transwell chamber; and connecting the other end to an injection pump; culturing alveolar epithelial cells and vascular endothelial cells on the front and back sides of the inverse opal membrane, respectively, to obtain the biomimetic microfluidic device. The biomimetic microfluidic device provided by this invention can achieve three-dimensional stretching under the action of a microfluidic injection pump, simulating the periodic stretch-recovery process of human lung respiratory movement in vitro. The fabrication method is simple and reliable.

The invention discloses a biological wave and negative oxygen ion synergistic blood purification device which comprises a barrel, the top end of the barrel is in threaded connection with a cover plate through bolts, one side of the top end of the cover plate fixedly communicates with a liquid inlet pipe, the middle of the bottom end of the barrel fixedly communicates with a liquid outlet pipe, and the top of the inner wall of the barrel is fixedly connected with a clamping ring. The bottom end of the clamping ring is fixedly connected with a liquid receiving hopper, the bottom end of the liquid receiving hopper is fixedly connected with a filtering cylinder, the bottom of the side, close to the liquid inlet pipe, of the cylinder body is fixedly communicated with a sampling pipe, one end of the sampling pipe is fixedly provided with an electromagnetic valve, and the bottom of the side, away from the liquid inlet pipe, of the cylinder body is fixedly provided with a water pump; by means of the filter screen, the micro-filtration membrane and the ultra-filtration membrane, the amount of pathogenic substances accumulated on each stage of filtration structure is reduced in a graded filtration mode, the blocking condition is reduced, blood can be repeatedly filtered till the requirement is met, and the purification effect is better.

The utility model discloses a degumming solution fine particle filtering device which comprises a device body, and a filter, a filtering water pipe and a filtering box which are arranged in the device body, one side of the device body is provided with a potassium permanganate solution input port, a drain outlet, a neutralizer input port, a DI water input port, a diaphragm pump air inlet, a compressed air input port and a degumming solution output port, the potassium permanganate solution input port and the drain outlet are respectively communicated with a filter, and the filter is internally provided with a high-precision filter membrane bag of which the filter hole size is 0.1-1.0 mu m; the high-precision filter membrane bag is communicated with the filter box through a filter water pipe, a diaphragm pump is arranged in the filter box, an air inlet of the diaphragm pump and a degumming solution output port are respectively communicated with the diaphragm pump, and a neutralizer input port and a DI water input port are respectively communicated with the filter. According to the utility model, fine particles in the potassium permanganate degumming liquid medicine can be effectively removed, and the cleanliness of the liquid medicine is ensured, so that the copper plating consistency of a PCB (Printed Circuit Board) is ensured.

The invention discloses a high-flux nylon needle type filter, and relates to the technical field of filters, the high-flux nylon needle type filter comprises a shell and a filter membrane arranged in the shell, the shell is provided with a liquid inlet pipe and a liquid outlet pipe, and the filter membrane divides the inner cavity of the shell into an upper chamber and a lower chamber; the filter membrane comprises an annular outer frame, a membrane sheet fixed in the outer frame in a tensioned manner, and a supporting net positioned below the membrane sheet; the supporting net is movably arranged in the outer frame and can rotate under the driving of filtering pressure, and the supporting net comprises an outer ring and an inner ring which are concentrically arranged in the outer frame, and a plurality of radial supporting strips for connecting the outer ring and the inner ring. According to the high-flux nylon needle type filter, the rotatable supporting net is introduced, and the shielding positions of the supporting strips on the filter membrane can be dynamically switched when the filter membrane is blocked, so that the inherent contradiction between the supporting reliability and the filtering flux is effectively balanced, and the improvement of the filtering efficiency is realized.

The invention discloses an automatic leaching control system for lead 212 radioactive isotopes. The leaching control system comprises a control system (1), a filtering device (2), a sampling accessory group (3), a column system (4) and a collecting accessory group (5), the control system comprises a first PLC controller (11) and a second PLC controller (12); the filter device (2) comprises a liquid level sensor (21), a stock solution cylinder (22), a centrifugal pump (23), a pressure sensor (24), a filter pump (25), a multi-layer filter membrane (26) and a filtrate cylinder (27); the sampling accessory group (3) comprises a six-way sampling valve (31), an acid bottle (32), an adjusting bottle (33), a second acid bottle (34), a sampling bottle (35), a three-way valve (36), a first four-way valve (37), a second four-way valve (38), a third four-way valve (39), a flow meter (30) and a second three-way valve (361); the column system comprises a sulfydryl modified silica gel chromatographic column (41), a diatomite column (42), a DTPA modified column (43), a second sulfydryl modified column (44) and an AG1-X8 resin column (45); the collecting accessory set comprises a first collecting bottle (51), a second collecting bottle (52), a six-way valve (53) and a lead collecting bottle set (54). The system is used for implementing the automatic leaching method for the lead 212 radioactive isotope.

The utility model discloses a filtering device for serum pretreatment, which relates to the technical field of filtering devices and comprises a cavity, the top of the cavity is in threaded connection with an upper cover, a sleeve plug is arranged in the middle of the inside of the cavity, and the outer surface of the top of the sleeve plug is sleeved with a stainless steel framework. A wire-wound filter element is arranged on the outer surface of the stainless steel framework, a feeding pipe is arranged at the top of the upper cover, the bottom of the feeding pipe extends to one side of the wire-wound filter element in the cavity, a clamping block is arranged at the top of the feeding pipe, a pressurizing pipe is arranged on one side of the cavity, and a bottom plate is arranged at the bottom end of the cavity; the bottom of the sleeve plug penetrates through the bottom plate of the cavity and extends to the bottom of the bottom plate. According to the filter device for serum pretreatment, a series of structures are arranged, so that serum can be completely filtered by the filter device for serum pretreatment, the filter membrane is prevented from being blocked by larger particles and impurities in the serum to influence the filtering effect, the filter device is convenient to disassemble and replace, and the operation steps are simple and efficient.

The utility model relates to the technical field of cell separation, and discloses a centrifugal tube for quickly separating peripheral blood bone marrow mononuclear cells, which comprises a centrifugal tube, an upper internal thread is arranged on the inner wall of the centrifugal tube, and a lower internal thread is arranged on the inner wall of the centrifugal tube. Blood and ficoll liquid are isolated through the filter membrane sheet and are prevented from being mixed too much to affect the separation effect, pure mononuclear cells can be simply, conveniently and rapidly collected in the centrifugal process due to the isolation effect of the inert separation gel and the blocking function of the filter membrane sheet, and after centrifugation is completed, the mononuclear cells are collected in a direct and rapid pouring mode, so that the separation effect is greatly improved. The inert separation gel is used for isolating centrifuged red blood cells, preventing the red blood cells from being mixed into separated mononuclear cells when supernatant is poured out, improving the separation method of the mononuclear cells, improving the separation efficiency of the mononuclear cells, reducing the experiment difficulty, saving the experiment time and guaranteeing the reliability of experiment results.

This invention discloses a highly efficient method for separating primary cells from cochlear tissue based on physical filtration. The method includes: S1: tissue pretreatment and viability preservation; S2: controlled mechanical dissociation; S3: automatic cell-impurity separation; b. differential centrifugation using a double-layer filtration centrifuge device; S4: impurity and cell removal and cell purification; S5: primary cell seeding and optimized culture. This separation method utilizes the natural differences in volume and mass between cells and bone fragments through a double-layer filtration centrifuge device and differential centrifugation filtration, simultaneously performing centrifugation sedimentation and filtration separation. Compared to traditional manual filtration or density gradient centrifugation, this device is simple to operate, has a fast separation speed, fundamentally avoids filter membrane clogging, and significantly improves separation efficiency, enabling the gentle and efficient separation of highly active primary cells from cochlear tissue.

The present invention provides an embedding container capable of providing an additional approach to methods for embedding cells and tissues. This embedding container 1 comprises: a filter 11 which has a surface on which an object 100 to be embedded by an embedding agent can be placed, and through which a chemical solution in which the object 100 is immersed can permeate; and a base material 10 that supports the filter 11 and accommodates the object 100. A space 12 that can be filled with the embedding agent is formed on the rear-surface side of the filter 11.