77 results about "Cell handling" patented technology

The centrifugal cup for cell treatment comprises a centrifugal cup body, a centrifugal cup head and a first flow guide pipe, the top of the centrifugal cup body is rotatably connected with the centrifugal cup head in a sealed mode along the longitudinal axis of the centrifugal cup, and a centrifugal treatment bin is arranged in the centrifugal cup body; an inlet, a first outlet and a second outlet are formed in the top of the centrifugal cup head, the bottom of the centrifugal cup head penetrates through the top of the centrifugal cup body and extends into the centrifugal cup body, and the top of the centrifugal cup body is rotatably connected with the middle of the centrifugal cup head in a sealed mode along the longitudinal axis of the centrifugal cup; the first flow guide pipe is arranged in the centrifugal cup body, the first end of the first flow guide pipe is fixedly connected with the inner side of the top of the centrifugal cup body, and the second end of the first flow guide pipe is arranged at the bottom of the centrifugal treatment bin and communicates with the outer edge of the centrifugal treatment bin; the inlet is communicated with the centrifugal treatment bin; the first outlet is communicated with the inner side of the centrifugal treatment bin through an interlayer flow channel in the side wall of the centrifugal cup body, and the interlayer flow channel is located above the outer edge; the second outlet is communicated with the outer edge through a first flow guide pipe.

A cell treatment system comprising: a liquid transfer unit for transferring a cell suspension from a first retention unit to a third retention unit via a branched connector, and transferring a cell treatment solution from a second retention unit to the third retention unit via the branched connector; and a transfer control unit for controlling the transfer of the cell suspension and the cell treatment solution by the liquid transfer unit so that the cell suspension and the cell treatment solution are combined at a prescribed ratio in the branched connector.

The present invention provides an AutoCell Platform (ACP), an advanced, fully automated, functionally closed system designed to revolutionize the manufacturing of genetically modified cell therapies. This innovative platform integrates automation, closed-loop processing, and novel technologies to address the inefficiencies and high costs of traditional methods. Key features include automated centrifugal seeding for enhanced genetic modification efficiency, microbubble-assisted cell selection for precise cell isolation, and a modular design for scalable amplification across clinical and research environments. By reducing production timelines from 30-40 days to less than 3 days and orders of magnitude in cost, the ACP enables instant manufacturing, decentralization, and broader accessibility. Advanced quality control measures and standardized design ensure compliance and consistent therapeutic outcomes. The ACP supports a variety of applications from CAR-T therapies to regenerative medicine, representing a transformative leap in global accessibility of precision medicine and life-saving cell therapies.

To reduce complication in incorporating a reagent into a cartridge for cell treatment.SOLUTION: A cartridge according to an embodiment is a cartridge for performing processing of cells therein, and includes a flow path through which a reagent used for the processing flows, a connection portion for connecting a reagent container containing the reagent to the flow path, and an opening / closing portion for connecting the reagent container to the connection portion and installing the reagent container in the cartridge.SELECTED DRAWING: Figure 4

The present invention provides a production method for iPS cells that uses a cell processing device. The cell processing device has a closed system portion at which a sealed container for material supply and a centrifugation chamber for counterflow centrifugation are connected by a connection conduit. The production method involves performing the following steps in order while keeping the closed system portion closed: a step (s1) for bringing an initialization factor into contact with somatic cells while performing counterflow centrifugation in the centrifugation chamber; and a step (s2) for establishing iPS cells from the somatic cells.

The present application relates to a method, an apparatus and a computer program product for wireless communication. The method comprises: receiving a candidate cell configuration for candidate cells, one or more first execution conditions associated with each of the candidate cells, and information associated with each of the candidate cells for subsequent cell addition / change, the information for subsequent cell addition / change is used for indicating candidate cell processing in a specific case and comprises at least one of the following: a list of subsequent / maintained candidate cells or one or more second execution conditions associated with each candidate cell in the list of subsequent / maintained candidate cells; storing the candidate cell configuration; evaluating one or more first execution conditions associated with each of the candidate cells; when a first execution condition associated with the target cell is satisfied, execution of a handover to a target cell selected from the candidate cells is performed.

Provided is a cell processing method including: preparing a cell suspension containing a cell to be processed and a target substance; and introducing the target substance into the cell to be processed by allowing a shear force to act on the cell to be processed, wherein the cell to be processed is a cell, which is selected from a cell group including cells in a proliferation process, and which has a cell diameter larger than a mode in a cell diameter distribution of the cell group.

The utility model relates to the technical field of photovoltaic module manufacturing, and particularly discloses a battery piece carrying module. The battery piece carrying module comprises a supporting frame, the supporting frame is connected with a ventilation sliding ring, a connecting piece is rotationally arranged in the ventilation sliding ring in a sealed mode, the top end of the connecting piece is connected with a first driving assembly driving the connecting piece to rotate relative to the ventilation sliding ring, and the bottom end of the connecting piece is connected with a mounting frame located below the supporting frame; two groups of suckers are arranged on the mounting frame; one group of suction cups are communicated with the interior of the ventilation slip ring through an air path, and the two air paths are not communicated with each other; the supporting frame is connected with a lifting driving assembly driving the supporting frame to move up and down. The battery piece carrying module can supply and carry the battery pieces at the two supply positions at the same time, and the carrying efficiency of the battery pieces can be improved.

The invention provides a cell product production instrument and a cell product production method using the same. The cell product production instrument comprises a whole blood sample bag; a magnetic separation device; the target cell processing module comprises a module main body, an accommodating cavity is formed in the module main body, a filter membrane is arranged in the accommodating cavity, the accommodating cavity is divided into a first space and a second space by the filter membrane, and a transfection reaction cavity capable of accommodating target cells is formed in the bottom of the first space; a heating module; a sample pumping assembly; the cleaning fluid pumping assembly is used for pumping the cleaning fluid in the cleaning fluid bag into the first space through the cleaning fluid inlet; the reagent pumping assembly is used for pumping a transfection reagent and / or a culture medium into the first space through the reagent inlet; and the cell product pumping assembly is used for conveying the transfected target cells in the first space into the finished blood bag through the cell product outlet. According to the invention, manual intervention and process breakpoints are thoroughly eliminated by realizing full-automatic internal circulation among the modules.

To provide an automatic culture device, an automatic culture system, and a culture method. Provided are an automatic culture device, an automatic culture system, and a culture method capable of accurately conveying a cell suspension between any containers without passing through a drive unit of a pump mechanism, regardless of the type of a cell culture container, a container containing the cell suspension, and a cell collection container to be used, and capable of efficiently culturing cells by conveying the cell suspension between any containers without passing through the drive unit of the pump mechanism. Cell supply and recovery with reduced physical damage to cells can be achieved. This automatic culture device is provided with: a cell suspension storage container capable of storing a cell suspension; a cell processing container for processing the cell suspension conveyed from the cell suspension storage container; a flow path connecting at least the cell suspension storage container and the cell processing container; a first buffer space connected to the flow path and having a predetermined pressure resistance; and a pressure source for introducing the cell suspension from the cell suspension storage container into the first buffer space by bringing the interior of the first buffer space into negative pressure.

Provided is a container for a plasma-type cell treatment apparatus that treats an object of interest with plasma, the container comprising: a container body; an electrode that is installed in an upper portion of the inside of the container body and generates plasma; a treatment unit that is provided in a lower portion of the inside of the container body; a flow path that allows the inside and outside of the container body to communicate with each other; and a variable mechanism that changes the relative position between the electrode and the treatment unit. The variable mechanism preferably has a connection part for connecting the container body to the electrode. The variable mechanism is preferably provided on a side surface of the container body.

The present disclosure provides systems and methods for measuring one or more analytes at the single cell level. In some instances, sequencing of a composite barcode sequence may identify a type of analyte, identify a cell, and determine that the type of analyte originated from the cell. In some instances, a probability that a type of analyte originated from the cell may be determined. Multiple types of analytes may be identified to have originated from the cell, and / or the respective probabilities determined. An analyte may be a protein, such as a surface-bound protein or an internal protein. An analyte may be a metabolite or other small molecule. An analyte may be any constituent of a cell.

There is provided an interface for coupling to a cell processing container having an internal volume for holding a cell suspension, the interface enabling sampling of material within he cell processing container. In particular, the interface includes a port configured to allow egress of a sample, and a conduit extending from said port so as to be located within the internal volume of the cell processing container and able to take a sample therefrom during use. The conduit has an internal bore and / or an inlet configured to minimise or mitigate agglomeration of cells during the sampling process.

Systems and method for separating, collecting, treating target cells and infusing the treated target cells to a patient are disclosed. The closed system includes a pre-defined fluid pathway providing flow communication between a whole blood source and said patient.

The present disclosure relates to cell processing technology. As an example, a cell processing system can include a plurality of sample processing devices configured to process patient samples and a plurality of readers associated with the plurality of sample processing devices, respectively, wherein each reader is configured to read information from a tracking device associated with a respective patient sample. The system can also include a controller that uses information from the readers to provide an estimated completion time for a patient sample based on availability of the sample processing devices.

The present invention relates to a system for collecting, separating, concentrating, and reinfusing blood components from whole blood. The system further includes cell modification. The system includes a blood processing device and a fluid flow circuit.

The invention relates to the technical field of biomedical treatment, and discloses a cell sorting and culturing method and device, computer equipment and a storage medium. The cell sorting and culturing method comprises the following steps: separating a sample solution by using a density gradient centrifugation method to obtain a to-be-cultured cell solution; conveying the to-be-cultured cell sap into culture equipment through first pipeline switching operation, and performing culture treatment on the to-be-cultured cell sap to obtain cultured cell sap; and obtaining a culture cell sap through a second switching pipeline operation, and concentrating and cleaning the culture cell sap to obtain a target cell sap. According to the invention, a totally-enclosed one-stop sterile operation environment can be ensured, the pollution risk during cell operation site change is avoided, and the cell treatment stability is improved. Meanwhile, continuous operation between sorting and culture treatment of the cell sap can be achieved through two times of pipeline switching operation, the automation degree and continuity of cell treatment are improved, the operation time is saved, and the cell treatment efficiency is improved.

The utility model provides a cell treatment consumable pipe group and cell treatment equipment. The cell treatment consumable pipe group comprises a centrifugal container, a first pipe group, a second pipe group, a first communicating pipeline, a magnetic separation column, a first separation pipeline and a second separation pipeline, one end of the third process first communicating pipeline communicates with the third process first pipe group and is located at a first communicating point, and the other end of the third process first communicating pipeline communicates with the third process second pipe group and is located at a second communicating point; the third process first separation pipeline, the third process magnetic separation column and the third process second separation pipeline are sequentially communicated, and the end, away from the third process magnetic separation column, of the third process first separation pipeline is communicated to the third process first pipe set and located at a third communication point. The end, away from the third process magnetic separation column, of the third process second separation pipeline communicates with the third process first pipe set and is located at a fourth communication point. According to the utility model, the pollution of cells is avoided, and the cell treatment efficiency is improved.

The application provides a cell product production instrument and a cell product production method using the same. The cell product production instrument comprises: a whole blood sample bag; a magnetic sorting device; a target cell processing module comprising a module main body, a containing cavity is formed in the module main body, a filter membrane is arranged in the containing cavity, the filter membrane separates the containing cavity into a first space and a second space, and the bottom of the first space is provided with a transfection reaction cavity capable of containing target cells; a heating module; a sample pumping assembly; a cleaning liquid pumping assembly for pumping cleaning liquid in a cleaning liquid bag into the first space through a cleaning liquid inlet pump; a reagent pumping assembly for pumping transfection reagents and / or culture medium into the first space through a reagent inlet pump; and a cell product pumping assembly for conveying the target cells in the first space after transfection into a finished product blood bag through a cell product outlet. The application completely eliminates manual intervention and process breakpoints by realizing full-automatic internal circulation among the modules.

The invention discloses a cell cryopreservation tube processing device, which comprises: a clamping mechanism, which comprises a storage rack and a clamping unit arranged on the storage rack, the top of the storage rack is provided with a storage hole for placing a container pipe fitting, and the clamping unit can abut against and fix the container pipe fitting placed in the storage hole; the centrifugal mechanism can bear the container pipe fitting and perform rotary centrifugation on the container pipe fitting; the culture mechanism comprises a culture box and a liquid shaking unit arranged in the culture box, and the liquid shaking unit is used for bearing and fixing the culture bottle and can control the culture bottle to swing in the culture box; the pipetting mechanism comprises a cap screwing unit for screwing a container pipe fitting or a culture bottle end cap and a pipetting unit for transferring a culture solution in the container pipe fitting or the culture bottle; according to the invention, the problems that the traditional cell cryopreservation tube treatment equipment lacks a synergistic effect in the multi-module working process of fixing, centrifuging, culturing and pipetting and cannot meet the requirements of high-throughput and high-precision cell treatment can be solved.

Described herein are methods for obtaining enucleated cells from nucleated cells. Also described herein are methods for cell processing, including providing a composition containing nucleated cells and enucleating at least a portion of the nucleated cells to produce an enucleated cell fraction. Also described herein are methods for cell processing, including expressing the heterologous gene product. Also provided are pharmaceuticals compositions comprising an enucleated cell.

A rocking assembly for mixing and cooling a liquid, such as a cell-containing liquid (e.g., a cell-based therapeutic product), comprises: a base (110) having a mount (120); a platform (130); a cooler module (150) operably connected to the platform and the mount, the cooler module configured to cool the platform; and an actuator operably connected with the chiller module, the actuator configured to move the platform in a rocking motion relative to the base. A cell processing system for filling and formulation processes in the manufacture of cell-based therapeutic agents, the cell processing system comprising the rocking assembly and a liquid dispensing system. A method of treating liquids for filling and formulation workflow applications.

The present invention provides a method for producing iPS cells using a cell processing apparatus. The cell processing apparatus has a closed system consisting of a sealed container for supplying materials and a centrifugation chamber configured to perform counterflow centrifugation, connected via a connecting pipe. The production method involves carrying out, in order, a step (s1) of contacting somatic cells with reprogramming factors and a step (s2) of establishing iPS cells from the somatic cells while performing counterflow centrifugation in the centrifugation chamber while maintaining the closed nature of the closed system.

This invention provides a system and method for processing biological samples. [Solution] The system comprises a support plate and at least one carriage assembly, the support plate having a central portion configured to reversibly interact with a motor for providing rotational force and at least one transverse arm, each of the at least one transverse arm including a first opening and at least one fixed opening, the at least one carriage assembly configured to reversibly interact with one of the at least one transverse arm, each of the at least one carriage assembly comprising a base portion and a tip assembly, the tip assembly comprising a microfluidic tip, a first sample chamber and a second sample chamber.

This cell treatment device is provided with: a factor introduction device 30 for introducing a pluripotent induction factor into cells so as to prepare induction factor-introduced cells; and a reprogramming suspension culture vessel for culturing the induction factor-introduced cells that have been prepared by the factor introduction device 30.

A cell processing platform and method for selecting and isolating target cells (e.g., T cells, NK cells, or hematopoietic stem cells) from a biological sample using functionalized microbubbles is provided. The platform employs microbubbles with a lipid shell functionalized with streptavidin and biotinylated linkers to bind specific target cells and make them buoyant in suspension. A centrifugation module separates non-target cells by sedimentation while maintaining the buoyancy of the target cell-microbubble complexes. A pneumatic control system disrupts the microbubbles at controlled pressure or frequency, releasing the target cells for downstream processing without compromising viability. Additional features include a microbubble generation module that can sequentially functionalize ligands for multi-step cell selection, and a temperature-controlled environment to ensure ligand stability. The system supports non-destructive cell release and sorting, enabling precise, contamination-free, and efficient cell isolation for therapeutic and research applications.

The utility model provides a cell treatment consumable pipe group and cell treatment equipment. The cell treatment consumable pipe group comprises a centrifugal container, a first pipe group, a second pipe group and a first communicating pipeline, wherein the first pipe group is communicated with the centrifugal container and used for being matched with a first driving pump; the second pipe group is communicated with the centrifugal container and used for being matched with a second driving pump; one end of the first communicating pipeline communicates with the first pipe set and is located at a first communicating point, and the other end of the first communicating pipeline communicates with the second pipe set and is located at a second communicating point. The first communication point and the centrifugal container are located on opposite sides of the first drive pump, and the second communication point and the centrifugal container are located on opposite sides of the second drive pump. According to the utility model, the pollution of cells is avoided, and meanwhile, the cell treatment efficiency is improved.

A method of transducing T cells includes providing a compressible container including a base, a top disposed substantially parallel to the base, and at least one flexible wall element extending between the top and the base and defining an interior volume of the compressible container. A population of T cells in a cell treatment medium is added to the internal volume. A transduction agent is added to the interior volume. The base of the compressible container moves relative to the top, causing turbulence of the contents in the interior volume during a first period of time.

The invention relates to the technical field of cell culture, and discloses a cell culture auxiliary device for cell culture, which comprises a material bag, an electric pinch valve connected below the material bag, a 22-channel conversion valve arranged below the electric pinch valve, a flowmeter sensor arranged below the left side of the 22-channel conversion valve, and a liquid level sensor arranged below the right side of the 22-channel conversion valve, the magnetic bead sorting device is arranged below the flow meter sensor, the peristaltic pump is arranged on the right side of the flow meter sensor, the eight-channel conversion valves are arranged below the magnetic bead sorting device, the number of the eight-channel conversion valves is three, the waste liquid collecting module is arranged below the magnetic bead sorting device, and the magnetic bead cleaning module is arranged on the right side of the waste liquid collecting module. The right side of the magnetic bead cleaning module is provided with a centrifugal structure, and the right side of the peristaltic pump is connected with a culture unit. The cell culture auxiliary device for cell culture has the advantages of integrating traditional dispersed cell treatment links into a continuous operation system, and solves the problems of dispersed cell culture process, slow process and low efficiency.