67 results about "Cell handling" patented technology

This disclosure concerns a cytometry system including a handling system that presents single cells to at least one quantum cascade laser (QCL) source. The QCL laser source is configured to deliver light to a cell within the cells in order to induce resonant mid-IR vibrational absorption by one or more analytes, leading to local heating that results in thermal expansion and an associated shockwave. An acoustic detection facility that detects the shockwave emitted by the single cell.

The invention features devices for mixing fluids, e.g., for lysing cells, and methods of use thereof. One device is based on the ability to control the flow of fluids, e.g., by contact angle and channel size. Fluids in this device can be divided to form segments of controlled volume, which are then brought together to initiate midxing. An exemplary use of the device is for the lysis of single cells. Another device is based on the ability to two mix two fluids in a channel and affinity capture of analytes. The devices can be integrated on the same chip with other devices, for example, for cell handling or analysis of DNA, rnRNA, and proteins released from the lysis of a cell.

Method and systems provide improved cell handling in microfluidic systems and devices using lateral cell trapping and methods of fabrication of the same that allow for selective low voltage electroporation and electrofusion.

The main objects of the present invention, which relates to regenerative medical treatments, are to enable (i) storage and conveyance of harvested or cultured cells without contamination occuring (ii) simple injection of the cells into a living body. To achieve these objects, cells harvested from a living body, or cells obtained by culturing harvested cells, are stored in a syringe-type storage vessel and subsequently transplanted into a living body. It is preferable that at least a part of the storage vessel inner wall in contact with the cells is formed from a cell non-adhesive material. Besides enabling cells in the vessel to take in the oxygen they require to survive, the present invention also enables cells quick and easy transplantation of cells into a living body without a cell detachment process, because cells are prevented from adhering to the inside of the vessel. Further, it is preferable that a stored tissue regeneration composition contains cell culture microcarriers floating in a fluidity medium, and that the cell culture microcarriers are composed of a bioabsorbable material and have cells adhering to their surfaces. Using this kind of tissue regeneration composition, a regenerative treatment can be carried out satisfactorily by simply and quickly transplanting cells from the syringe-type cell storage vessel into a living body without intricate scaffold-related procedures being required.

The invention relates to an unicell detector based on a nano fiber probe, which comprises a nano probe, a light source unit, a micro operation system, an electricity detection unit, a cellular localization system and a photon detection unit, an innermost layer of the nano probe is a fiber layer, the outer wall of the fiber layer is wrapped with a nano ring electrode layer, and the outer wall of the nano ring electrode layer is wrapped with an insulating layer. A manufacturing method of the unicell detector based on the nano fiber probe comprises the steps of drawing, sputtering, making the insulating layer and cutting focused ion beam. The unicell detector has high sensitivity and can realize the unicell grade detection, compared with a traditional detection means by crushing millions of cells, the required cell sample amount is greatly reduced, and the success rate of disease detection at early stage can be increased. The unicell detector can perform in-vivo cell detection and can avoid the false appearance generation during a cell processing process. The space-time resolution and detectable target object scope can be greatly enhanced, and a biochemistry mechanism in the unicell enables real-time detection and analysis.

The embodiment of the invention provides a physical resource allocation method and physical resource allocation equipment. The physical resource allocation method comprises the steps that: user equipment receives a system broadcast message or a radio resource control protocol RRC reconfiguration message sent by a base station, and the system broadcast message or the RRC reconfiguration message is used for indicating that the base station supports the user equipment at a first ability category to carry out 64 quadrature amplitude modulation QAM on uplink data; the user equipment sends the ability category and uplink modulation information of the user equipment to the base station, the uplink modulation information is used for indicating that the user equipment is supportive of carrying out the 64 QAM on the uplink data, wherein the ability category of the user equipment belongs to the first ability category, so that the base station can allocate physical resources to the user equipment according to the ability category and the uplink modulation information of the user equipment; and the user equipment receives the physical resources allocated to the user equipment by the base station, utilizes the physical resources and reports data to the base station in a 64QAM modulation manner. The physical resource allocation method and the physical resource allocation equipment can enhance cell handling capacity.

The invention provides a method for preparing a calcium alginate-fat acellular matrix microcarrier by applying a micro-fluidic chip. The method specifically comprises the following steps: subjecting human adipose tissue to repeated freeze thawing, performing emulsifying to obtain nano fat, performing centrifuging to remove upper-layer oil and lower-layer water, subjecting the middle layer to decellularization with a PBS buffer solution, a trypsin-EDTA digestive solution, isopropanol and a mixed enzyme solution, performing soaking, extracting and centrifuging with an alpha-amylase aqueous solution and acetic acid to obtain supernatant, mixing the supernatant with a sodium alginate solution with the same volume, taking the obtained mixture as a dispersed phase, introducing the mixture into amicro-fluidic chip, introducing n-decanol containing span-80 as a continuous phase into micro-fluidic chip to obtain acellular matrix-sodium alginate droplets coated by the n-decanol, finally introducing an n-decanol solution containing CaCl2 for crosslinking overnight, and washing off n-decanol to obtain the calcium alginate-fat acellular matrix microcarrier. The method can produce the acellularmatrix microcarrier with uniform components, and the obtained microcarrier is more beneficial to cell growth and can be used for culturing adipose-derived stem cells.

A cell treatment system includes an analysis unit to calculate the area or size of each of cell aggregates existing on the cell culture vessel based on an image; a display device to show the area or size of each of the cell aggregates on a display; a laser irradiator to emit. laser toward the cell culture vessel so as to kill cells existing on the cell culture vessel; an input device to receive an operation input designating cells to be killed with irradiation of the laser or cells to survive without the irradiation of the laser among the cells existing on the cell culture vessel, or an operation input designating a position to be irradiated with the laser or a position not to be irradiated with the laser on the cell culture vessel; and a control unit to control a position irradiated with the laser,

A cell treatment apparatus capable of treating cells in a cell culture vessel. The cell treatment apparatus (100) according to the present invention includes a first region (1), a second region (3), and a third region (5). The first region (1) and the second region (3) are placed in succession. The first region (1) is a cell treatment chamber for treating cells. The cell treatment chamber can be closed from the outside of the cell treatment chamber and includes a culture vessel placement portion for placing a cell culture vessel. The second region (3) includes a laser irradiation device capable of irradiating the cell culture vessel placed in the culture vessel placement portion with a laser. The third region (5) includes a control device that controls at least one device in the cell treatment apparatus (100) and a power supply device (52) that supplies electric power to at least one device in the cell treatment apparatus (100). The culture vessel placement portion is placed to be adjacent to the second region (3) in the cell treatment chamber. An adjacent portion to the second region (3) in the culture vessel placement portion is translucent.

Provided is a microfluidic chip for physicochemically treating a single cell whose diameter is 50-400 micrometers. The body of the microfluidic chip comprises a fluidic layer, a pneumatic layer, and an elastic film sandwiched between the fluidic layer and the pneumatic layer; the fluidic layer comprises a switch-back single cell treating and retrieving channel, a first reagent inflow channel, a second reagent inflow channel, a reagent mixing channel, a third reagent inflow channel, a backwash channel, and a negative pressure and waste liquid sharing channel; the pneumatic layer comprises a pneumatic micro-valve group for controlling the opening and closing of each channel in the fluidic layer; the first reagent inflow channel and the second reagent inflow channel are separately connected with the inlet of the reagent mixing channel; the reagent mixing channel, the third reagent inflow channel, the backwash channel, and the negative pressure and waste liquid sharing channel are separately connected with the switch-back single cell treating and retrieving channel; and the third reagent inflow channel and the backwash channel are separately independent channels or share one channel. Also provided is a microfluidic device comprising the microfluidic chip and a method for physicochemically treating a single cell by using the microfluidic chip and the microfluidic device.

The invention relates to sample storing liquid applicable to parasitic ovum detection and a use method thereof. The sample storing liquid applicable to parasitic ovum detection is prepared through malachite green, a pH buffering agent, sodium dodecyl sulfate, paraformaldehyde, methyl alcohol, ethyl alcohol, glycerinum, L-alanine, etc. The sample storing liquid has the main beneficial effects that(1) the storing liquid is applicable to storage and treatment of human and animal waste; the cost is low; the sample can be stored for a long term and is conveniently conveyed on the premise that theparasitic ovum is kept complete; the detection environment is improved; the form of the parasitic ovum stored in the liquid-based cell handling liquid can be fixed and maintained, and the parasitic ovum is prevented from swelling and breaking and atrophy, and thus, the convenience of diagnosing is ensured; and (2) the sample storing liquid contains the components which enable soft viewing field background and reduction of vision fatigue, and thus the convenience of observing is ensured.

Provided are methods, systems, and kits for cell processing, e.g., for therapeutic use, such as for adoptive cell therapy. The provided methods include transduction methods, in which cells and virus are incubated under conditions that result in transduction of the cells with a viral vector. The incubation in some embodiments is carried out in an internal cavity of a generally rigid centrifugal chamber, such as a cylindrical chamber made of hard plastic, the cavity of which may have a variable volume. The methods include other processing steps, including those carried out in such a chamber, including washing, selection, isolation, culture, and formulation. In particular, the disclosure relates to method providing advantages over available processing methods, such as available methods for large-scale processing. Such advantages include, for example, reduced cost, streamlining, increased efficacy, increased safety, and increased reproducibility among different subjects and conditions.

The present invention provides improved methods, facilities and systems for parallel processing of biological cellular samples in an efficient and scalable manner. The invention enables parallel processing of biological cellular samples, such as patient samples, in a space and time efficient fashion. Process simulation may be used to determine the optimal arrangement and/or quantity of cell processing equipment needed. The methods, facilities and systems of the invention find particular utility in processing patient samples for use in cell therapy.