44 results about "Cell handling" patented technology

The centrifugal cup for cell treatment comprises a centrifugal cup body, a centrifugal cup head and a first flow guide pipe, the top of the centrifugal cup body is rotatably connected with the centrifugal cup head in a sealed mode along the longitudinal axis of the centrifugal cup, and a centrifugal treatment bin is arranged in the centrifugal cup body; an inlet, a first outlet and a second outlet are formed in the top of the centrifugal cup head, the bottom of the centrifugal cup head penetrates through the top of the centrifugal cup body and extends into the centrifugal cup body, and the top of the centrifugal cup body is rotatably connected with the middle of the centrifugal cup head in a sealed mode along the longitudinal axis of the centrifugal cup; the first flow guide pipe is arranged in the centrifugal cup body, the first end of the first flow guide pipe is fixedly connected with the inner side of the top of the centrifugal cup body, and the second end of the first flow guide pipe is arranged at the bottom of the centrifugal treatment bin and communicates with the outer edge of the centrifugal treatment bin; the inlet is communicated with the centrifugal treatment bin; the first outlet is communicated with the inner side of the centrifugal treatment bin through an interlayer flow channel in the side wall of the centrifugal cup body, and the interlayer flow channel is located above the outer edge; the second outlet is communicated with the outer edge through a first flow guide pipe.

To reduce complication in incorporating a reagent into a cartridge for cell treatment.SOLUTION: A cartridge according to an embodiment is a cartridge for performing processing of cells therein, and includes a flow path through which a reagent used for the processing flows, a connection portion for connecting a reagent container containing the reagent to the flow path, and an opening / closing portion for connecting the reagent container to the connection portion and installing the reagent container in the cartridge.SELECTED DRAWING: Figure 4

To provide an automatic culture device, an automatic culture system, and a culture method. Provided are an automatic culture device, an automatic culture system, and a culture method capable of accurately conveying a cell suspension between any containers without passing through a drive unit of a pump mechanism, regardless of the type of a cell culture container, a container containing the cell suspension, and a cell collection container to be used, and capable of efficiently culturing cells by conveying the cell suspension between any containers without passing through the drive unit of the pump mechanism. Cell supply and recovery with reduced physical damage to cells can be achieved. This automatic culture device is provided with: a cell suspension storage container capable of storing a cell suspension; a cell processing container for processing the cell suspension conveyed from the cell suspension storage container; a flow path connecting at least the cell suspension storage container and the cell processing container; a first buffer space connected to the flow path and having a predetermined pressure resistance; and a pressure source for introducing the cell suspension from the cell suspension storage container into the first buffer space by bringing the interior of the first buffer space into negative pressure.

Provided is a container for a plasma-type cell treatment apparatus that treats an object of interest with plasma, the container comprising: a container body; an electrode that is installed in an upper portion of the inside of the container body and generates plasma; a treatment unit that is provided in a lower portion of the inside of the container body; a flow path that allows the inside and outside of the container body to communicate with each other; and a variable mechanism that changes the relative position between the electrode and the treatment unit. The variable mechanism preferably has a connection part for connecting the container body to the electrode. The variable mechanism is preferably provided on a side surface of the container body.

Systems and method for separating, collecting, treating target cells and infusing the treated target cells to a patient are disclosed. The closed system includes a pre-defined fluid pathway providing flow communication between a whole blood source and said patient.

The present disclosure relates to cell processing technology. As an example, a cell processing system can include a plurality of sample processing devices configured to process patient samples and a plurality of readers associated with the plurality of sample processing devices, respectively, wherein each reader is configured to read information from a tracking device associated with a respective patient sample. The system can also include a controller that uses information from the readers to provide an estimated completion time for a patient sample based on availability of the sample processing devices.

The present invention relates to a system for collecting, separating, concentrating, and reinfusing blood components from whole blood. The system further includes cell modification. The system includes a blood processing device and a fluid flow circuit.

The invention relates to the technical field of biomedical treatment, and discloses a cell sorting and culturing method and device, computer equipment and a storage medium. The cell sorting and culturing method comprises the following steps: separating a sample solution by using a density gradient centrifugation method to obtain a to-be-cultured cell solution; conveying the to-be-cultured cell sap into culture equipment through first pipeline switching operation, and performing culture treatment on the to-be-cultured cell sap to obtain cultured cell sap; and obtaining a culture cell sap through a second switching pipeline operation, and concentrating and cleaning the culture cell sap to obtain a target cell sap. According to the invention, a totally-enclosed one-stop sterile operation environment can be ensured, the pollution risk during cell operation site change is avoided, and the cell treatment stability is improved. Meanwhile, continuous operation between sorting and culture treatment of the cell sap can be achieved through two times of pipeline switching operation, the automation degree and continuity of cell treatment are improved, the operation time is saved, and the cell treatment efficiency is improved.

The utility model provides a cell treatment consumable pipe group and cell treatment equipment. The cell treatment consumable pipe group comprises a centrifugal container, a first pipe group, a second pipe group, a first communicating pipeline, a magnetic separation column, a first separation pipeline and a second separation pipeline, one end of the third process first communicating pipeline communicates with the third process first pipe group and is located at a first communicating point, and the other end of the third process first communicating pipeline communicates with the third process second pipe group and is located at a second communicating point; the third process first separation pipeline, the third process magnetic separation column and the third process second separation pipeline are sequentially communicated, and the end, away from the third process magnetic separation column, of the third process first separation pipeline is communicated to the third process first pipe set and located at a third communication point. The end, away from the third process magnetic separation column, of the third process second separation pipeline communicates with the third process first pipe set and is located at a fourth communication point. According to the utility model, the pollution of cells is avoided, and the cell treatment efficiency is improved.

The application provides a cell product production instrument and a cell product production method using the same. The cell product production instrument comprises: a whole blood sample bag; a magnetic sorting device; a target cell processing module comprising a module main body, a containing cavity is formed in the module main body, a filter membrane is arranged in the containing cavity, the filter membrane separates the containing cavity into a first space and a second space, and the bottom of the first space is provided with a transfection reaction cavity capable of containing target cells; a heating module; a sample pumping assembly; a cleaning liquid pumping assembly for pumping cleaning liquid in a cleaning liquid bag into the first space through a cleaning liquid inlet pump; a reagent pumping assembly for pumping transfection reagents and / or culture medium into the first space through a reagent inlet pump; and a cell product pumping assembly for conveying the target cells in the first space after transfection into a finished product blood bag through a cell product outlet. The application completely eliminates manual intervention and process breakpoints by realizing full-automatic internal circulation among the modules.

The invention discloses a cell cryopreservation tube processing device, which comprises: a clamping mechanism, which comprises a storage rack and a clamping unit arranged on the storage rack, the top of the storage rack is provided with a storage hole for placing a container pipe fitting, and the clamping unit can abut against and fix the container pipe fitting placed in the storage hole; the centrifugal mechanism can bear the container pipe fitting and perform rotary centrifugation on the container pipe fitting; the culture mechanism comprises a culture box and a liquid shaking unit arranged in the culture box, and the liquid shaking unit is used for bearing and fixing the culture bottle and can control the culture bottle to swing in the culture box; the pipetting mechanism comprises a cap screwing unit for screwing a container pipe fitting or a culture bottle end cap and a pipetting unit for transferring a culture solution in the container pipe fitting or the culture bottle; according to the invention, the problems that the traditional cell cryopreservation tube treatment equipment lacks a synergistic effect in the multi-module working process of fixing, centrifuging, culturing and pipetting and cannot meet the requirements of high-throughput and high-precision cell treatment can be solved.

Described herein are methods for obtaining enucleated cells from nucleated cells. Also described herein are methods for cell processing, including providing a composition containing nucleated cells and enucleating at least a portion of the nucleated cells to produce an enucleated cell fraction. Also described herein are methods for cell processing, including expressing the heterologous gene product. Also provided are pharmaceuticals compositions comprising an enucleated cell.

A rocking assembly for mixing and cooling a liquid, such as a cell-containing liquid (e.g., a cell-based therapeutic product), comprises: a base (110) having a mount (120); a platform (130); a cooler module (150) operably connected to the platform and the mount, the cooler module configured to cool the platform; and an actuator operably connected with the chiller module, the actuator configured to move the platform in a rocking motion relative to the base. A cell processing system for filling and formulation processes in the manufacture of cell-based therapeutic agents, the cell processing system comprising the rocking assembly and a liquid dispensing system. A method of treating liquids for filling and formulation workflow applications.

The present invention provides a method for producing iPS cells using a cell processing apparatus. The cell processing apparatus has a closed system consisting of a sealed container for supplying materials and a centrifugation chamber configured to perform counterflow centrifugation, connected via a connecting pipe. The production method involves carrying out, in order, a step (s1) of contacting somatic cells with reprogramming factors and a step (s2) of establishing iPS cells from the somatic cells while performing counterflow centrifugation in the centrifugation chamber while maintaining the closed nature of the closed system.

This invention provides a system and method for processing biological samples. [Solution] The system comprises a support plate and at least one carriage assembly, the support plate having a central portion configured to reversibly interact with a motor for providing rotational force and at least one transverse arm, each of the at least one transverse arm including a first opening and at least one fixed opening, the at least one carriage assembly configured to reversibly interact with one of the at least one transverse arm, each of the at least one carriage assembly comprising a base portion and a tip assembly, the tip assembly comprising a microfluidic tip, a first sample chamber and a second sample chamber.

The utility model provides a cell treatment consumable pipe group and cell treatment equipment. The cell treatment consumable pipe group comprises a centrifugal container, a first pipe group, a second pipe group and a first communicating pipeline, wherein the first pipe group is communicated with the centrifugal container and used for being matched with a first driving pump; the second pipe group is communicated with the centrifugal container and used for being matched with a second driving pump; one end of the first communicating pipeline communicates with the first pipe set and is located at a first communicating point, and the other end of the first communicating pipeline communicates with the second pipe set and is located at a second communicating point. The first communication point and the centrifugal container are located on opposite sides of the first drive pump, and the second communication point and the centrifugal container are located on opposite sides of the second drive pump. According to the utility model, the pollution of cells is avoided, and meanwhile, the cell treatment efficiency is improved.

The invention relates to the technical field of cell culture, and discloses a cell culture auxiliary device for cell culture, which comprises a material bag, an electric pinch valve connected below the material bag, a 22-channel conversion valve arranged below the electric pinch valve, a flowmeter sensor arranged below the left side of the 22-channel conversion valve, and a liquid level sensor arranged below the right side of the 22-channel conversion valve, the magnetic bead sorting device is arranged below the flow meter sensor, the peristaltic pump is arranged on the right side of the flow meter sensor, the eight-channel conversion valves are arranged below the magnetic bead sorting device, the number of the eight-channel conversion valves is three, the waste liquid collecting module is arranged below the magnetic bead sorting device, and the magnetic bead cleaning module is arranged on the right side of the waste liquid collecting module. The right side of the magnetic bead cleaning module is provided with a centrifugal structure, and the right side of the peristaltic pump is connected with a culture unit. The cell culture auxiliary device for cell culture has the advantages of integrating traditional dispersed cell treatment links into a continuous operation system, and solves the problems of dispersed cell culture process, slow process and low efficiency.

This invention discloses a photovoltaic module arrangement device, arrangement method, production system, and production method. The photovoltaic module arrangement device includes: a support unit for supporting a substrate assembly, the substrate assembly including a lower rigid plate and a lower flexible layer; a cell loading unit for moving multiple cells to corresponding positions on the substrate assembly, the cell loading unit being movable relative to the substrate assembly supported by the support unit; and a solder ribbon loading unit for moving multiple solder ribbons to corresponding positions on the substrate assembly, the solder ribbon loading unit being movable relative to the substrate assembly supported by the support unit. This photovoltaic module arrangement device reduces the number of cell handling operations, thereby improving the yield and production continuity of the module production line.

The application discloses a cell treatment method based on sinusoidal alternating current stimulation, and the method comprises the following steps: applying sinusoidal alternating current stimulation to target stem cells, and the mitochondrial membrane potential, ATP synthesis and aerobic respiration level of the stem cells treated by the electric stimulation are improved. The application further discloses a cell stimulation device based on sinusoidal alternating current stimulation, and the device comprises a cell culture container and a planar interdigital electrode substrate placed in the cell culture container, wherein the planar interdigital electrode substrate comprises an optically transparent substrate and metal planar interdigital electrodes on the substrate. The application discloses the application of the stem cells treated by the above method or the stem cells treated by the device in the preparation of a medicine for mitochondrial transplantation treatment. The application improves the mitochondrial membrane potential, ATP synthesis capacity and aerobic respiration level of the cells by applying sinusoidal alternating current stimulation to the target stem cells.

The present application discloses a tissue cell treatment method and apparatus, an electronic device, and a storage medium. The method comprises: on the basis of a stain image of a target object, determining a labeled region of each tissue type contained in the stain image; according to the cell size of each tissue type, determining the size of a sub-region to be treated corresponding to each labeled region; further performing grid processing on each labeled region to obtain grid coordinates; and extracting corresponding data from gene expression data and overlaying the data on the grid coordinates to obtain a tissue cell treatment result. Since the cell sizes of different tissues are inconsistent, the cell sizes corresponding to different tissue types can be differentiated according to the size of said sub-region, thus generating data of grids of different sizes in different labeled regions to simulate the pseudo cell sizes of different cell tissues, so as to implement tissue-level cell analysis; the obtained analysis result can accurately reflect tissue information of a sample, thus improving the accuracy of the analysis result, and achieving a better analysis effect.

This disclosure provides systems, devices, and methods for mechanoporosis of cells to facilitate the delivery of payloads into cells. By providing a disposable tube set that is compatible with existing pumps and other cell processing equipment and is operable at the low pressures provided by these existing pump systems, the mechanoporosis system described herein can simplify cell processing systems. The tube set can mix a cell suspension received from an upstream cell processing equipment with a payload optionally contained within the tube set, and the cell membranes can be disturbed by passing the cells through the cell disturbance filter of the tube set.

Described herein are improved microfluidic devices and methods for processing cells that can improve cell quality, streamline workflows, and lower costs. Applications include research and clinical diagnostics in cancer, infectious disease, and inflammatory disease, among other disease areas.

To provide a cell treatment system in which damage to cells is reduced when a cell suspension and a cell treatment liquid are mixed.SOLUTION: A cell treatment system comprising: a liquid moving part configured to move a cell suspension from a first storage part to a third storage part through a branch joint and move a cell treatment solution from a second storage part to the third storage part through the branch joint; and a movement control part configured to control movement of the cell suspension and the cell treatment solution by the liquid moving part so that the cell suspension and the cell treatment solution join at the branch joint at a predetermined ratio.SELECTED DRAWING: Figure 1

Methods for obtaining enucleated cells from nucleated cells are described herein. Methods for cell processing, comprising providing a composition containing nucleated cells and enucleating at least a portion of nucleated cells to produce an enucleated cell fraction, are also described herein. Methods for cell processing, comprising expressing heterologous gene products, are also described herein. Pharmaceutical compositions containing enucleated cells are also provided.

This invention belongs to the field of battery cell handling technology and discloses a TOPcon battery cell handling support device, including a fixed plate. An adjustment component is provided on the outer side of the fixed plate. The adjustment component includes an adjustment screw, and an adjustment sleeve is slidably provided on the outer side of the adjustment screw. By setting the adjustment component and the fixed plate and other structures, this invention facilitates the lateral sliding adjustment of the outer adsorption structures along the limiting bracket through the central adjustment structure. During the adjustment process, the distance between the suction cup structure and the central fixed plate can be changed, thereby changing the adsorption size according to the needs. At the same time, by setting a fixed spring to cooperate with the adsorption column to slide up and down along the inner side of the adjustment slider, auxiliary buffering is provided to avoid the problem of excessive pressure causing damage to the battery cells. This achieves flexible adjustment and uses the fixed spring to push the adsorption column to reset, while avoiding excessive pressure causing damage to the battery cells.

The application relates to the technical field of cell drying, and particularly discloses a dehydration drying method of cells and a cell dehydration drying preparation prepared by the method. The dehydration drying method of cells disclosed by the application specifically comprises the following steps: resuspending the cells in a drying protective agent, and performing vacuum dehydration drying treatment, so that the cell dehydration drying preparation is obtained; the specific steps of the vacuum dehydration drying treatment are as follows: placing a sample under the condition that the temperature is 15-40 DEG C and the first vacuum degree is 2-8 Torr, and keeping for 10-30 min; keeping the temperature unchanged, vacuumizing to the second vacuum degree of 0.3-1.2 Torr, and keeping for 20-45 min. The cells are treated by using the drying method, and the cell dehydration drying preparation is prepared, so that the operation is simple, the advantages of short time, high efficiency, low energy consumption and low cost are achieved, the cell dehydration and death can be effectively avoided, the cell recovery rate is improved, and the product quality of the cell drying preparation is ensured.

To provide a novel method for producing iPS cells that requires less patient-derived somatic cells as raw material and reduces the use of reagents (e.g., viral vectors) compared to conventional methods. [Solution] The present invention provides a method for producing iPS cells using a cell processing device. The cell processing device has a closed system portion in which a sealed container for supplying materials and a centrifugal chamber configured to perform counterflow centrifugal separation are connected via a connecting conduit. The production method involves performing a step (s1) of contacting somatic cells with reprogramming factors while performing counterflow centrifugal separation in the centrifugal chamber, while maintaining the closed nature of the closed system portion.

The embodiment of the invention provides a bifunctional micro-fluidic chip for guiding cancer medication, a preparation method and a detection method. The chip comprises a substrate, a cell processing layer and a substance injection layer which are sequentially stacked, the substance injection layer is communicated with the cell treatment layer; the cell processing layer comprises a cell mutation detection module and an organoid analysis module, and the substance injection layer comprises a drug injection module; the cell mutation detection module is used for capturing and detecting the type of injected cancer cells; the drug injection module is used for providing a corresponding anti-cancer drug for the organoid analysis module according to the type of injected cancer cells; and the organoid analysis module is used for culturing the injected cancer cells into organoid and monitoring the medication reaction of the organoid in real time. According to the chip, a feasible solution combining cell EGFR mutation detection and organoid-based drug reaction test is realized, and the chip can be used for guiding personalized lung cancer drug treatment.