119 results about "Cell handling" patented technology

The present disclosure relates to systems, devices, and methods for automated cell sorting within a cell processing system. In an embodiment, the present disclosure relates to an automated cell sorting system comprising a cartridge having a cell sorting module, where the cell sorting module comprises a flow cell, and an instrument within a bay of a cell processing workcell, where the instrument comprises a magnetic array couplable to the flow cell, each of the magnets within the magnetic array having a width of w and being spaced apart by between about w / 3 to about ¾w, and wherein the flow cell has a height of between about 1 / 12w to about ⅛w.

The centrifugal cup for cell treatment comprises a centrifugal cup body, a centrifugal cup head and a first flow guide pipe, the top of the centrifugal cup body is rotatably connected with the centrifugal cup head in a sealed mode along the longitudinal axis of the centrifugal cup, and a centrifugal treatment bin is arranged in the centrifugal cup body; an inlet, a first outlet and a second outlet are formed in the top of the centrifugal cup head, the bottom of the centrifugal cup head penetrates through the top of the centrifugal cup body and extends into the centrifugal cup body, and the top of the centrifugal cup body is rotatably connected with the middle of the centrifugal cup head in a sealed mode along the longitudinal axis of the centrifugal cup; the first flow guide pipe is arranged in the centrifugal cup body, the first end of the first flow guide pipe is fixedly connected with the inner side of the top of the centrifugal cup body, and the second end of the first flow guide pipe is arranged at the bottom of the centrifugal treatment bin and communicates with the outer edge of the centrifugal treatment bin; the inlet is communicated with the centrifugal treatment bin; the first outlet is communicated with the inner side of the centrifugal treatment bin through an interlayer flow channel in the side wall of the centrifugal cup body, and the interlayer flow channel is located above the outer edge; the second outlet is communicated with the outer edge through a first flow guide pipe.

The invention relates to the technical field of environmental toxicology, in particular to a construction method and application of micro-nano plastic combined toxicity (AOP) based on multi-omics integration, and the construction method comprises the following steps: respectively carrying out single exposure culture of multi-target risk substances by adopting human-derived hepatocyte HepaRG cells; determining a combined exposure dose; cell treatment and sample preparation; transcriptome analysis; analyzing metabolome; transcriptome and metabolome data are integrated, pathway cross analysis is carried out, a gene-metabolite interaction network is constructed, and a core regulation and control module is identified; and constructing an AOP framework: determining a molecular initial event, a key event and a harmful outcome of the AOP framework based on the integrated data, and importing an AOP-WIKI platform to generate a standardized AOP framework. According to the method, the limitation of a traditional single omics and fixed dose model is broken through, full-chain dynamic association from a molecular initial event to a harmful outcome is realized, and a scientific basis is provided for combined pollution risk assessment in the fields of environment and food.

A cell processing platform and method for selecting and isolating target cells, such as T-cells, NK-cells, or hematopoietic stem cells, from a biological sample using functionalized microbubbles. The platform employs microbubbles with lipid shells, functionalized with streptavidin and biotinylated linkers, to bind specific target cells and render them buoyant within the suspension. A centrifugation module separates non-target cells by sedimentation while maintaining the buoyancy of target cell-microbubble complexes. A pneumatic control system disrupts the microbubbles under controlled pressure or frequency, releasing the target cells for downstream processing without compromising viability. Additional features include a microbubble generation module capable of sequentially functionalizing ligands for multi-step cell selection and a temperature-controlled environment to ensure ligand stability. The system supports non-destructive cell release and sorting, enabling precise, contamination-free, and high-efficiency cell isolation for therapeutic and research applications.

The invention relates to the technical field of biological engineering, and particularly discloses an integrated cell treatment super-clean bench, which comprises a super-clean bench body with a fan system, and a pipetting mechanism, an uncovering mechanism, a centrifugal mechanism and a pipe cap opener which are arranged in the super-clean bench body, the super clean bench further comprises a first feeding and discharging mechanism and a second feeding and discharging mechanism, the first feeding and discharging mechanism is used for feeding / discharging culture dishes into / out of the super clean bench body through the feeding and discharging channel, and the second feeding and discharging mechanism is used for feeding / discharging test tubes into / out of the super clean bench body through the feeding and discharging channel. According to the scheme, the problem that a culture dish storage area and a test tube storage area are arranged in the super clean bench and occupy a large space in integrated equipment is solved.

A cell treatment system comprising: a liquid transfer unit for transferring a cell suspension from a first retention unit to a third retention unit via a branched connector, and transferring a cell treatment solution from a second retention unit to the third retention unit via the branched connector; and a transfer control unit for controlling the transfer of the cell suspension and the cell treatment solution by the liquid transfer unit so that the cell suspension and the cell treatment solution are combined at a prescribed ratio in the branched connector.

The present invention provides an AutoCell Platform (ACP), an advanced, fully automated, functionally closed system designed to revolutionize the manufacturing of genetically modified cell therapies. This innovative platform integrates automation, closed-loop processing, and novel technologies to address the inefficiencies and high costs of traditional methods. Key features include automated centrifugal seeding for enhanced genetic modification efficiency, microbubble-assisted cell selection for precise cell isolation, and a modular design for scalable amplification across clinical and research environments. By reducing production timelines from 30-40 days to less than 3 days and orders of magnitude in cost, the ACP enables instant manufacturing, decentralization, and broader accessibility. Advanced quality control measures and standardized design ensure compliance and consistent therapeutic outcomes. The ACP supports a variety of applications from CAR-T therapies to regenerative medicine, representing a transformative leap in global accessibility of precision medicine and life-saving cell therapies.

To reduce complication in incorporating a reagent into a cartridge for cell treatment.SOLUTION: A cartridge according to an embodiment is a cartridge for performing processing of cells therein, and includes a flow path through which a reagent used for the processing flows, a connection portion for connecting a reagent container containing the reagent to the flow path, and an opening / closing portion for connecting the reagent container to the connection portion and installing the reagent container in the cartridge.SELECTED DRAWING: Figure 4

The present invention provides a production method for iPS cells that uses a cell processing device. The cell processing device has a closed system portion at which a sealed container for material supply and a centrifugation chamber for counterflow centrifugation are connected by a connection conduit. The production method involves performing the following steps in order while keeping the closed system portion closed: a step (s1) for bringing an initialization factor into contact with somatic cells while performing counterflow centrifugation in the centrifugation chamber; and a step (s2) for establishing iPS cells from the somatic cells.

The present application relates to a method, an apparatus and a computer program product for wireless communication. The method comprises: receiving a candidate cell configuration for candidate cells, one or more first execution conditions associated with each of the candidate cells, and information associated with each of the candidate cells for subsequent cell addition / change, the information for subsequent cell addition / change is used for indicating candidate cell processing in a specific case and comprises at least one of the following: a list of subsequent / maintained candidate cells or one or more second execution conditions associated with each candidate cell in the list of subsequent / maintained candidate cells; storing the candidate cell configuration; evaluating one or more first execution conditions associated with each of the candidate cells; when a first execution condition associated with the target cell is satisfied, execution of a handover to a target cell selected from the candidate cells is performed.

The invention provides a biological laboratory single cell treatment instrument, which is characterized in that a cell culture area of a culture dish is covered with a limiting assembly, the limiting assembly comprises a culture cover covering the culture area, the edge of the culture cover is uniformly provided with a plurality of quantification grooves, and the inside of each quantification groove is movably connected with a quantification assembly; compared with the prior art, when single cells are treated, the single cells are quantified through the quantification tank and the quantification assembly, a large number of single cell treatment quantification samples are obtained through the quantification assembly at the same time, when an experiment is carried out, only usable samples need to be obtained from the quantification samples, and the single cells can be used repeatedly through one-time cultivation.

Provided is a cell processing method including: preparing a cell suspension containing a cell to be processed and a target substance; and introducing the target substance into the cell to be processed by allowing a shear force to act on the cell to be processed, wherein the cell to be processed is a cell, which is selected from a cell group including cells in a proliferation process, and which has a cell diameter larger than a mode in a cell diameter distribution of the cell group.

The utility model relates to the technical field of photovoltaic module manufacturing, and particularly discloses a battery piece carrying module. The battery piece carrying module comprises a supporting frame, the supporting frame is connected with a ventilation sliding ring, a connecting piece is rotationally arranged in the ventilation sliding ring in a sealed mode, the top end of the connecting piece is connected with a first driving assembly driving the connecting piece to rotate relative to the ventilation sliding ring, and the bottom end of the connecting piece is connected with a mounting frame located below the supporting frame; two groups of suckers are arranged on the mounting frame; one group of suction cups are communicated with the interior of the ventilation slip ring through an air path, and the two air paths are not communicated with each other; the supporting frame is connected with a lifting driving assembly driving the supporting frame to move up and down. The battery piece carrying module can supply and carry the battery pieces at the two supply positions at the same time, and the carrying efficiency of the battery pieces can be improved.

The invention provides a cell product production instrument and a cell product production method using the same. The cell product production instrument comprises a whole blood sample bag; a magnetic separation device; the target cell processing module comprises a module main body, an accommodating cavity is formed in the module main body, a filter membrane is arranged in the accommodating cavity, the accommodating cavity is divided into a first space and a second space by the filter membrane, and a transfection reaction cavity capable of accommodating target cells is formed in the bottom of the first space; a heating module; a sample pumping assembly; the cleaning fluid pumping assembly is used for pumping the cleaning fluid in the cleaning fluid bag into the first space through the cleaning fluid inlet; the reagent pumping assembly is used for pumping a transfection reagent and / or a culture medium into the first space through the reagent inlet; and the cell product pumping assembly is used for conveying the transfected target cells in the first space into the finished blood bag through the cell product outlet. According to the invention, manual intervention and process breakpoints are thoroughly eliminated by realizing full-automatic internal circulation among the modules.

To provide an automatic culture device, an automatic culture system, and a culture method. Provided are an automatic culture device, an automatic culture system, and a culture method capable of accurately conveying a cell suspension between any containers without passing through a drive unit of a pump mechanism, regardless of the type of a cell culture container, a container containing the cell suspension, and a cell collection container to be used, and capable of efficiently culturing cells by conveying the cell suspension between any containers without passing through the drive unit of the pump mechanism. Cell supply and recovery with reduced physical damage to cells can be achieved. This automatic culture device is provided with: a cell suspension storage container capable of storing a cell suspension; a cell processing container for processing the cell suspension conveyed from the cell suspension storage container; a flow path connecting at least the cell suspension storage container and the cell processing container; a first buffer space connected to the flow path and having a predetermined pressure resistance; and a pressure source for introducing the cell suspension from the cell suspension storage container into the first buffer space by bringing the interior of the first buffer space into negative pressure.

Provided is a container for a plasma-type cell treatment apparatus that treats an object of interest with plasma, the container comprising: a container body; an electrode that is installed in an upper portion of the inside of the container body and generates plasma; a treatment unit that is provided in a lower portion of the inside of the container body; a flow path that allows the inside and outside of the container body to communicate with each other; and a variable mechanism that changes the relative position between the electrode and the treatment unit. The variable mechanism preferably has a connection part for connecting the container body to the electrode. The variable mechanism is preferably provided on a side surface of the container body.

The present disclosure provides systems and methods for measuring one or more analytes at the single cell level. In some instances, sequencing of a composite barcode sequence may identify a type of analyte, identify a cell, and determine that the type of analyte originated from the cell. In some instances, a probability that a type of analyte originated from the cell may be determined. Multiple types of analytes may be identified to have originated from the cell, and / or the respective probabilities determined. An analyte may be a protein, such as a surface-bound protein or an internal protein. An analyte may be a metabolite or other small molecule. An analyte may be any constituent of a cell.

1. The name of this design product: experimental cabin. 2. Purpose of this design product: This design product is used in experimental chambers for cell processing work. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the design points: Stereoscopic diagram 1.

The current subject matter provides a cell engineering platform for vector-free and / or viral delivery of payload / cargo compounds and compositions into cells. The platform achieves delivery to cells quickly and in an easy to use manner. Related apparatus, systems, techniques, articles and compositions are also described.

A functionally closed cell processing platform designed to minimize contamination and automate cell processing. The platform integrates multiple specialized cassettes, including a reagent sample cassette (RSC) for reagent delivery and sample collection, a process fluids cassette (PFC) for waste and buffer management, and a cell processing cassette (CPC) for cell handling, along with a transfer syringe cassette (TSC) for precise fluid transfer. The system features a centrifuge capable of multi-mode operation for cell concentration and mixing, pneumatic controls for fluid and microbubble manipulation, and UV sanitizers to maintain aseptic conditions. Advanced functionalities include optical and thermal sensors for real-time monitoring, automated control of fluid and reagent handling, and a microbubble cell selection system for precise target cell isolation. This innovative platform ensures efficient, contamination-free cell processing, supporting applications in gene therapy, cell manufacturing, and biomedical research.

There is provided an interface for coupling to a cell processing container having an internal volume for holding a cell suspension, the interface enabling sampling of material within he cell processing container. In particular, the interface includes a port configured to allow egress of a sample, and a conduit extending from said port so as to be located within the internal volume of the cell processing container and able to take a sample therefrom during use. The conduit has an internal bore and / or an inlet configured to minimise or mitigate agglomeration of cells during the sampling process.

The present field relates generally to devices for the treatment of single cells or small cell masses, in particular for the treatment of single mammalian cells or mammalian cell masses, methods of making the same, and methods of using the same. The device is generally a single cell treatment device for treating a single cell during a microinjection procedure, comprising: a base vessel having an upper surface and a lower surface; an array of micro-pores comprising one or more micro-pores, the one or more micro-pores comprising: at least one micro-pore wall defining an upper micro-pore opening and a central pore, the at least one micro-pore wall having two protruding cell-stabilizing protrusions formed therein, each cell-stabilizing protrusion defining cavities positioned opposite each other on a common horizontal plane; and the groove is used for maintaining fluid therein, is positioned on the upper surface of the base vessel and extends around the periphery of the micro-pore array.

A cell processing platform and method for selective isolation and activation of target cells, such as T-cells, NK-cells, and hematopoietic stem cells, from a biological sample. The platform employs aptamer-functionalized microbubbles, which selectively bind to target cell surface antigens to create buoyant target cell-microbubble complexes. A centrifugation module separates buoyant target cells from non-target cells by exploiting their distinct buoyancy properties. The system includes advanced aptamer designs, such as Locked Nucleic Acid (LNA) modifications, temperature-controlled folding buffers, and reversible binding mechanisms using complementary strands or enzymatic cleavage to release target cells. Additional features include optimized spacer configurations to enhance binding accessibility and aptamer stability for high specificity and yield. This platform also enables selective removal of senescent cells using aptamer-functionalized microbubbles, facilitating continuous processing and supporting applications in cell therapy, research, and regenerative medicine.

The disclosed invention presents the AutoCell Platform (ACP), an advanced, fully automated, and functionally closed system designed to revolutionize the manufacturing of gene-modified cell therapies. This innovative platform integrates automation, closed-loop processing, and novel technologies to address the inefficiencies and high costs of conventional methods. Key features include automated spinoculation for enhanced genetic modification efficiency, microbubble-assisted cell selection for precise cell isolation, and modular design for scalability across clinical and research settings. By reducing production timelines from 30-40 days to under 3 days and lowering costs by an order of magnitude, the ACP enables point-of-care manufacturing, decentralization, and broader accessibility. Advanced quality control measures and a standardized design ensure regulatory compliance and consistent therapeutic outcomes. The ACP supports diverse applications, from CAR-T therapies to regenerative medicine, representing a transformative leap in precision medicine and the global accessibility of life-saving cell-based therapies.

Systems and method for separating, collecting, treating target cells and infusing the treated target cells to a patient are disclosed. The closed system includes a pre-defined fluid pathway providing flow communication between a whole blood source and said patient.

Multi version library cell handling and integrated circuit structures fabricated therefrom are described. In an example, an integrated circuit structure includes a plurality of gate lines parallel along a first direction of a substrate and having a pitch along a second direction orthogonal to the first direction. A first version of a cell type is over a first portion of the plurality of gate lines, the first version of the cell type including a first plurality of interconnect lines having a second pitch along the second direction, the second pitch less than the first pitch.

The present disclosure relates to cell processing technology. As an example, a cell processing system can include a plurality of sample processing devices configured to process patient samples and a plurality of readers associated with the plurality of sample processing devices, respectively, wherein each reader is configured to read information from a tracking device associated with a respective patient sample. The system can also include a controller that uses information from the readers to provide an estimated completion time for a patient sample based on availability of the sample processing devices.

The present invention relates to a system for collecting, separating, concentrating, and reinfusing blood components from whole blood. The system further includes cell modification. The system includes a blood processing device and a fluid flow circuit.

An apparatus includes a cartridge for holding a consumable kit and a dock for holding an instrument. The cartridge includes first docking members and first locking members, and the dock includes second docking members and second locking members. The first and second docking members are removably coupled to each other, establishing a single degree of freedom motion for moving the cartridge relative to the dock. The first and second locking members are selectively coupled to each other, selectively locking the cartridge with the dock in the single degree of freedom motion. The apparatus facilities automated loading of the consumable kit to the instrument.