Unicell detector based on nano fiber probe and its probe manufacturing method

Abstract

The invention relates to an unicell detector based on a nano fiber probe, which comprises a nano probe, a light source unit, a micro operation system, an electricity detection unit, a cellular localization system and a photon detection unit, an innermost layer of the nano probe is a fiber layer, the outer wall of the fiber layer is wrapped with a nano ring electrode layer, and the outer wall of the nano ring electrode layer is wrapped with an insulating layer. A manufacturing method of the unicell detector based on the nano fiber probe comprises the steps of drawing, sputtering, making the insulating layer and cutting focused ion beam. The unicell detector has high sensitivity and can realize the unicell grade detection, compared with a traditional detection means by crushing millions of cells, the required cell sample amount is greatly reduced, and the success rate of disease detection at early stage can be increased. The unicell detector can perform in-vivo cell detection and can avoid the false appearance generation during a cell processing process. The space-time resolution and detectable target object scope can be greatly enhanced, and a biochemistry mechanism in the unicell enables real-time detection and analysis.

Description

technical field [0001] The invention relates to the application of electrical and optical technologies in the biological field, in particular to a single-cell detector and a method for making probes thereof. Background technique [0002] In recent years, with the gradual deepening of research on cell physiology, scientists have discovered that even with the same genetic material and the same surrounding environment, these cells will still develop in different directions, and sometimes produce different functions. Individual cells appear to be the same, but their chemical composition and stress response are very different, and changes in individual cells are also related to major diseases such as cancer and neurological diseases. Single-celled biological processes in plants and animals are also far from fully understood. Single-cell analysis is therefore an important means of understanding and deciphering many critical life processes. However, the size of a single cell is v...

AI Technical Summary

Problems solved by technology

However, the size of a single cell is very small, and the chemical components contained in it are also ultra-trace. However, the current analysis method Western blot needs to break millions of cells to achieve the detection of intracellular biochemical indicators. Therefore, traditional analysis Method is insufficient to detect differences at the level of individual cells

Not only that, the traditional methods are mostly fixed-point detection, which cannot realize real-time monitoring

Images