7results about How to "High spatio-temporal resolution" patented technology

This invention relates to the field of biomedical detection technology, and discloses a bitetrahedral framework nucleic acid TDN cascade amplification system and its application, including TDN-1 and TDN-2. TDN-1 has a hairpin H1 containing a fluorescent reporter group attached to its surface, and TDN-2 has a hairpin H2 attached to its surface. The sequences of H1 and H2 are designed to undergo an alternating hybridization chain reaction triggered by target mRNA. This scheme relies on the synergistic effect of TDN structural anchoring and precise hairpin probe sequence design to overcome the technical defects of traditional free HCR hairpins in living cells, such as poor stability, low reaction efficiency, and non-specific triggering, achieving more efficient and specific intracellular signal amplification. Furthermore, it enables highly specific and sensitive in-situ imaging and dynamic tracking of low-abundance mRNAs such as c-Myc in single living cells, effectively solving the problems of insufficient detection sensitivity and false positives caused by the inability of traditional detection methods to effectively enrich targets and the low efficiency and high background of traditional HCR and other signal amplification technologies in intracellular applications.

The application provides a mechanism model and deep learning-based reservoir bloom spatiotemporal prediction method and device, relates to the land surface water environment prediction technical field, and includes the following steps: acquiring basic data of a target reservoir bay; constructing a three-dimensional water dynamics and water environment mechanism model based on the basic data, and performing bloom growth and decline simulation to construct a spatiotemporal continuous bloom prediction data set; constructing a bloom spatiotemporal prediction model, training the bloom spatiotemporal prediction model based on the bloom prediction data set; and predicting the bloom of the target reservoir bay based on the trained bloom spatiotemporal prediction model. The method and device provided by the application construct a three-dimensional water dynamics and water environment mechanism model based on basic data, perform bloom growth and decline simulation, and provide high-quality and high-density training data; construct a bloom spatiotemporal prediction model that integrates an attention mechanism, a graph convolutional neural network and a long short-term memory network, and realize high-precision and high-spatiotemporal-resolution rapid prediction of the bloom condition of the entire reservoir water area.

This invention discloses a method for real-time observation of biofilm growth on the surface of microplastics of different particle sizes. The method includes: preparing a microfluidic chip comprising a microchannel network layer and a transparent substrate layer bonded together; the microchannel network layer comprising one or more microchannel network units; each microchannel network unit comprising two or more interception cavities for intercepting microplastics of different particle sizes, with the particle size of the intercepted microplastics gradually decreasing from the inlet to the outlet; injecting a suspension containing microplastics of different particle sizes; injecting bacterial culture and liquid culture medium; placing the microfluidic chip on the stage of a microscope; and observing and photographing the biofilm growth in each interception cavity in real time during the culture process. Using the method and dedicated microfluidic chip provided by this invention, simultaneous interception and localization of microplastics of different particle sizes can be achieved. Combined with fluorescent labeling and microscopic imaging techniques, in-situ, real-time, and dynamic observation of the formation, development, and succession of biofilms on the surface of microplastics can be realized.

The invention relates to the technical field of cement performance detection, in particular to a detection method for measuring chemical shrinkage of neat cement paste, which comprises the following steps: pouring and sealing neat cement paste with a specified mixing ratio in an annular sample cavity with a flexible transparent cavity wall. And the annular sample cavity is horizontally immersed in the constant-temperature liquid medium tank. A laser emitting device is used for projecting a circular laser spot to the outer side of the flexible cavity wall of the annular sample cavity, and an image acquisition device is used for continuously acquiring a spot deformation image sequence caused by deformation of the cavity wall along with contraction of the internal neat paste. Light spot deformation parameters are analyzed and calculated based on the image sequence, then the volume change amount of the cement paste sample in the annular sample cavity is obtained through inversion, and the chemical shrinkage value is calculated by combining the initial volume. According to the invention, non-contact, continuous and high-precision measurement of the chemical shrinkage process of the cement paste is realized, the operation is simple and convenient, and the external interference is small.

This invention discloses a method for dynamically detecting the morphology of a cavity based on interlayer fracture vibrations, belonging to the field of cavity construction and monitoring technology for salt cavern gas storage. The method involves drilling and trenching to locate the cavity center and mudstone interlayer, deploying monitoring points and burying distributed monitoring optical fibers in the outer region of the cavity wall to capture the vibration wave signals of the interlayer fracture, recording the time difference and fluctuation intensity differences; analyzing the signal data of the propagation path to establish a three-dimensional coordinate system; and establishing a system of equations based on the time difference between the primary fracture vibration and the secondary vibration from the rock impact to calculate the three-dimensional coordinates of the fracture point and characteristic points of the cavity wall; then reconstructing the real-time contour of the cavity based on the layered symmetrical fracture characteristics and signal energy characteristics, and verifying it with other signals from the fracture layer and correcting the accuracy of the monitoring equipment. This method does not require interruption of the cavity construction operation, has high spatiotemporal resolution, and can accurately invert the cavity morphology, providing a basis for dynamic adjustment of the flow velocity and direction in water-soluble cavity construction, effectively reducing the risk of cavity collapse and excessive lateral dissolution.

The invention discloses a concentration field imaging device and method, relates to the field of gas concentration detection, and relates to gas leakage detection imaging and rapid traceability. The device comprises an optical modulation module, a micro galvanometer, a laser emission module, a single-point concentration measurement module and a data processing imaging module. The azimuth of the emergent laser is instantly modulated, motion noise in the scanning process is suppressed, and random sampling of scene concentration and concentration field inversion are realized in combination with a compressed sensing theory. According to the concentration field imaging device and method, millisecond-level light beam deflection regulation and control of emergent laser are achieved through the multiple birefringent prism regulation and control units, and the light spot static retention time in the laser wavelength modulation process is maximized. Noise interference caused by scanning motion to single-point concentration measurement is suppressed, the concentration measurement speed is increased, the reconstructability of a coefficient concentration field is ensured by combining a compressed sensing theory and using random scanning, and the temporal-spatial resolution of concentration field imaging is improved.

The present application relates to a kind of mouse sleep fragmentation research system based on electrophysiological electrochemical synchronous recording, belong to animal experiment equipment technical field, solve the lack of electrophysiological, electrochemical signal synchronous recording function in the prior art in the process of modeling;Cannot assess the influence of modeling degree and neurochemistry level one of the problems etc..The present application includes sleep fragmentation modeling module, signal measurement and acquisition control module and analysis module;Sleep fragmentation modeling module provides sleep space for mouse, and interval predetermined time is carried out to mouse sleep interference;Signal measurement and acquisition control module is used to measure and collect the electrophysiological signal and electrochemical signal of disturbed mouse;Analysis module is used to assess sleep quality and modeling effect.The present application realizes the integrated design of mouse sleep fragmentation modeling, electrophysiological signal recording, electrochemical signal detection, provides complete experimental system for the neurochemical mechanism research of sleep fragmentation.