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Sample storing liquid applicable to parasitic ovum detection

A technology for parasite eggs and preservation solution, applied in the field of sample preservation solution, can solve the problems of heavy odor, cannot be stored for a long time, time-consuming, etc., and achieves the effects of improving environment, good fixation and maintenance, and easy observation.

Inactive Publication Date: 2018-07-24
博朗(厦门)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used methods to detect parasite eggs, such as direct inspection of worms in feces, saline smear method, Kato method, etc., but these methods are limited by certain conditions, such as samples cannot be stored for a long time, and the odor is heavy during detection. time consuming

Method used

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  • Sample storing liquid applicable to parasitic ovum detection
  • Sample storing liquid applicable to parasitic ovum detection
  • Sample storing liquid applicable to parasitic ovum detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of the sample preservation solution that is used for parasite egg detection

[0023] Prepare the sample preservation solution for the detection of parasite eggs according to the following formula, and the composition of each 1000mL is as follows:

[0024] Paraformaldehyde: 50g;

[0025] Methanol: 15mL;

[0026] Ethanol: 30mL;

[0027] L-alanine: 0.5g;

[0028] L-isoleucine: 0.5g;

[0029] Phosphate buffer: 0.01mol / L;

[0030] Sodium lauryl sulfate: 0.15g;

[0031] Vanillin: 7.5g;

[0032] Malachite green: 0.07g;

[0033] Glycerin: 5g;

[0034] The balance is water, and the pH value is adjusted to 6.7.

[0035] The method for detecting parasite eggs in human feces using the sample preservation solution for detection of parasite eggs, the method comprising:

[0036] (1) 2g of feces samples are placed in 10mL of the sample preservation solution for the detection of parasite eggs to obtain a mixture;

[0037] (2) mix the mixture of s...

Embodiment 2

[0041] Embodiment 2: the preparation of the sample preservation solution that is used for parasite egg detection

[0042] Prepare the sample preservation solution for the detection of parasite eggs according to the following formula, and the composition of each 1000mL is as follows:

[0043] Paraformaldehyde: 80g;

[0044] Methanol: 30mL;

[0045] Ethanol: 30mL;

[0046] L-alanine: 0.6g;

[0047] L-isoleucine: 0.6g;

[0048] Phosphate buffer: 0.012mol / L;

[0049] Sodium lauryl sulfate: 0.2g;

[0050] Vanillin: 8g;

[0051] Malachite green: 0.08g;

[0052] Glycerin: 30g;

[0053] The balance is water, and the pH value is adjusted to 6.4.

[0054] figure 2 It is the feces of animals treated with the sample preservation solution for detection of parasite eggs in this embodiment (the detection method is the same as in Example 1). Observation is convenient for clinical testing.

Embodiment 3

[0055] Embodiment 3: the preparation of the sample preservation solution that is used for parasite egg detection

[0056] Prepare the sample preservation solution for the detection of parasite eggs according to the following formula, and the composition of each 1000mL is as follows:

[0057] Paraformaldehyde: 70g;

[0058] Methanol: 20mL;

[0059] Ethanol: 20mL;

[0060] L-alanine: 0.4g;

[0061] L-isoleucine: 0.4g;

[0062] Phosphate buffer: 0.009mol / L;

[0063] Sodium lauryl sulfate: 0.12g;

[0064] Vanillin: 5g;

[0065] Malachite green: 0.07g;

[0066] Glycerin: 40g;

[0067] The balance is water, and the pH value is adjusted to 6.5.

[0068] image 3It is the feces of animals treated with the sample preservation solution for the detection of parasite eggs in this embodiment (the detection method is the same as in Example 1), and the detected eggs have a clear background in the figure, and the shape of the eggs is maintained well, which is easy to observe. The d...

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Abstract

The invention relates to sample storing liquid applicable to parasitic ovum detection and a use method thereof. The sample storing liquid applicable to parasitic ovum detection is prepared through malachite green, a pH buffering agent, sodium dodecyl sulfate, paraformaldehyde, methyl alcohol, ethyl alcohol, glycerinum, L-alanine, etc. The sample storing liquid has the main beneficial effects that(1) the storing liquid is applicable to storage and treatment of human and animal waste; the cost is low; the sample can be stored for a long term and is conveniently conveyed on the premise that theparasitic ovum is kept complete; the detection environment is improved; the form of the parasitic ovum stored in the liquid-based cell handling liquid can be fixed and maintained, and the parasitic ovum is prevented from swelling and breaking and atrophy, and thus, the convenience of diagnosing is ensured; and (2) the sample storing liquid contains the components which enable soft viewing field background and reduction of vision fatigue, and thus the convenience of observing is ensured.

Description

(1) Technical field [0001] The invention relates to a sample preservation solution for detection of parasite eggs and a method for detecting parasite eggs in feces by using the sample preservation solution for detection of parasite eggs. (2) Background technology [0002] Parasite eggs invade the host, which can cause various hazards and affect the health of the host. Parasite eggs can be excreted with the feces of the host, so the presence of parasites and the degree of infection can be detected through feces. Commonly used methods to detect parasite eggs, such as direct inspection of worms in feces, saline smear method, Kato method, etc., but these methods are limited by certain conditions, such as samples cannot be stored for a long time, and the odor is heavy during detection. Time consuming to wait. (3) Contents of the invention [0003] In view of the deficiencies of the existing fecal parasite detection technology, the purpose of the present invention is to provid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00G01N21/84
CPCA01N1/00G01N21/84
Inventor 魏兴江
Owner 博朗(厦门)生物科技有限公司
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