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Preparation method for collagen-graphene oxide-adipose acellular matrix microcarrier

A technology of acellular matrix and collagen, which is applied in the field of tissue engineering bio-scaffold micro-carriers, can solve the problems of difficult preparation and achieve the effect of facilitating cell growth

Inactive Publication Date: 2019-04-26
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the fat acellular matrix has a flocculent structure, which is difficult to prepare as a carrier

Method used

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  • Preparation method for collagen-graphene oxide-adipose acellular matrix microcarrier
  • Preparation method for collagen-graphene oxide-adipose acellular matrix microcarrier
  • Preparation method for collagen-graphene oxide-adipose acellular matrix microcarrier

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Embodiment Construction

[0036] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0037] The capillary microfluidic chip in the following examples is prepared according to the following method: nest glass capillaries with an inner diameter of 100 um and an inner diameter of 500 um for about 3 cm, and then glue the glass slide 1 with epoxy glue, and fix the inner layer Both ends of the capillary 2 and the nested end of the outer capillary 3 and the inner capillary 2 are placed on the slide 1, the non-nested end of the outer capillary 3 protrudes from the slide 1, and the inner capillary 2 is not nested end and the nested end of the outer capillary 3 and the inner capillary 2 respectively buckle the tip 4 of the 10ul sampling gun, and the bottom edge of the tip 4 of the sampling gun is sealed and bonded to the glass slide 1 by epoxy glue, and after connection, the The undercut areas of the tip 4 of the two sampling guns respectively fo...

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Abstract

The invention provides a preparation method for a collagen-graphene oxide-adipose acellular matrix microcarrier. The method specifically comprises the following steps: taking a human adipose tissue, repeatedly freezing and thawing, and then emulsifying to obtain fat; centrifuging and removing upper layer oil and lower layer water; taking an intermediate layer, and performing decellularized treatment by adopting a PBS (Phosphate buffer solution), trypsase-EDTA digestive juice, isopropyl alcohol and a mixed enzyme solution; then homogenating by using an alpha-amylase water solution and acetic acid to obtain an adipose acellular matrix solution, and mixing with a collagen-graphene oxide solution to be used as a dispersion phase for feeding into a micro-fluidic chip; and using n-decyl alcoholcontaining span-80 as a continuous phase for feeding into the micro-fluidic chip to obtain an acellular matrix-collagen-graphene oxide liquid drop wrapped with the n-decyl alcohol, and crosslinking through crosslinking liquid to obtain the microcarrier. The method provided by the invention is capable of generating the acellular matrix microcarrier with homogeneous components; and the obtained microcarrier is more beneficial to cell growth, and can be used for culturing adipose stem cell.

Description

technical field [0001] The invention belongs to tissue engineering biological scaffold microcarriers, in particular to a method for constructing collagen-graphene oxide-fat decellularized matrix microcarriers using microfluidic chips, which can increase the adhesion of fat stem cells and is a new microcarrier . Background technique [0002] Soft tissue defect is a common clinical problem. Existing methods such as flap transplantation, fat filling, and hyaluronic acid filling have disadvantages such as trauma, easy absorption of fillers, and inflammatory reactions. The method of tissue engineering combines seed cells with bio-scaffold materials for filling, which can largely avoid these problems. However, currently commonly used bio-scaffold materials such as chitosan, fibrin, and hyaluronic acid do not have the ability to promote stem cell adhesion and proliferation. , The ability to differentiate. [0003] Acellular adipose matrix is ​​the remaining part of adipose tissu...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/08A61L27/24A61L27/50
CPCA61L27/08A61L27/24A61L27/3633A61L27/3641A61L27/3687A61L27/3691A61L27/50A61L2430/34A61L2430/40
Inventor 张馨月汪振星孙家明周牧冉罗超陈雳风曾宇阳侯金飞牟珊黎媛方慧敏王冰倩齐钰涵张郭
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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