3D printing collagen biological sponge scaffold and preparation method thereof

A technology of collagen and original protein, which is applied in the direction of non-active components of polymer compounds, prostheses, additive processing, etc., can solve the problems of low viscosity of pure collagen, difficult to print and shape, etc., to facilitate cell growth and maintain survival rate , the effect of high cell viability

Pending Publication Date: 2021-06-08
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the viscosity of pure collagen

Method used

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  • 3D printing collagen biological sponge scaffold and preparation method thereof
  • 3D printing collagen biological sponge scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0038] Example 1

[0039] The recombinant human source protein was dissolved in a aqueous solution of 0.12 M acetic acid, and 3% (wt%) recombinant human gum were prepared; the chitosan was dissolved in a aqueous solution of chitosan in a aqueous solution of acetic acid, formulated into 3% (wt%) shell. Glycan solution; additionally, the original sputum is dissolved in phosphate buffer (pH = 6) containing 10% (V / V) anhydrous ethanol (pH = 6), and is equipped with 0.3% (wt%) of the original sputin solution.

[0040] Under ice bath conditions, the chitosan solution was dripped into a recombinant human gum protein solution, and the mixture was stirred until the mass ratio of the recombinant human collagen and chitosan was 1: 1, and the total content was 1.5% (wt%). ), Continued for 20 minutes, vacuum-defoated with vacuum defoaming machine, then injecting a mixed solution into the print cartridge, print the freezing form layer layer by layer by the CAD model at -30 ° C, placed in a va...

Example Embodiment

[0041] Example 2

[0042]The recombinant human source protein was dissolved in a aqueous solution of 0.12 M acetic acid, and 3% (wt%) recombinant human gum were prepared; the chitosan was dissolved in a aqueous solution of chitosan in a aqueous solution of acetic acid, formulated into 3% (wt%) shell. Glycan solution; additionally, the original sputum is dissolved in phosphate buffer (pH = 6) containing 10% (V / V) anhydrous ethanol (pH = 6), and is equipped with 0.3% (wt%) of the original sputin solution.

[0043] Under ice bath conditions, the chitosan solution was dripped into a recombinant human gum protein solution, and the mixture was stirred during the period until the mass ratio of the recombinant human gum and the mass ratio of chitosan was 4: 1, and the total content was 1.5% (wt%). When stirring was continued for 20 minutes, it was vacuum with vacuum defoaming machine, and then the mixed solution was injected into the print cartridge, and the freezing form layer was prin...

Example Embodiment

[0044] Example 3

[0045] The recombinant human source protein was dissolved in a aqueous solution of 0.12 M acetic acid, and 3% (wt%) recombinant human gum were prepared; the chitosan was dissolved in a aqueous solution of chitosan in a aqueous solution of acetic acid, formulated into 3% (wt%) shell. Glycan solution; additionally, the prototypes were dissolved in phosphate buffer (pH 6) containing 10% (V / V) anhydrous ethanol (pH 6), and was equipped with 0.3% (wt%) of the original sputin solution.

[0046] Under ice bath conditions, the chitosan solution was dripped into a recombinant human gelatin solution, and the mixture was stirred until the mass ratio of the recombinant human gelatin and chitosan was 1: 4, and the total content was 1.5% (wt%). When stirring was continued for 20 minutes, it was vacuum with vacuum defoaming machine, and then the mixed solution was injected into the print cartridge, and the freezing form layer was printed by the CAD model at -30 ° C, and then...

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Abstract

The invention discloses a 3D printing collagen biological sponge scaffold and a preparation method thereof. The method comprises the steps that firstly, an acid solution containing chitosan and an acid solution containing collagen are mixed to be uniform to prepare 3D printing bio-ink, then the bio-ink is added into a printing gun head, and printing forming is conducted in a low-temperature environment according to design parameters and a model; and freeze-drying treatment is conducted on the freeze-formed product, the freeze-dried product is soaked in a solution containing procyanidine for cross-linking immobilization, and finally freeze-drying is performed after washing to obtain the recombinant human collagen biological sponge scaffold. By means of a low-temperature deposition 3D printing technology, all-environment temperature control can be achieved, and the forming stability is improved. The biological sponge scaffold has proper pores, forming controllability, good physical and chemical properties and biocompatibility, does not contain other impurities, and is low in manufacturing cost.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and relates to a 3D printed collagen biological sponge support and a preparation method thereof. Background technique [0002] Collagen is the most common protein in the extracellular matrix (ECM) of animals. It is a triple helical structure composed of three intertwined polypeptide chains, which helps the growth and proliferation of cells in the body. However, pure collagen has a very low viscosity and is difficult to print. Therefore, it is usually necessary to add other substances to collagen to improve its viscosity problem. Chitosan is a naturally derived polysaccharide with excellent biodegradability, antifungal and antibacterial activities, and is non-cytotoxic. In addition, chitosan also contains the GAG ​​structural unit of N-acetyl-D-glucosamine, which is a good 3D printing material. In natural ECM, collagen is combined with glycosaminoglycans to improve its structural p...

Claims

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Application Information

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IPC IPC(8): A61L27/24A61L27/20A61L27/56A61K47/42C08J9/28C08J9/40C08L5/08C08L89/00B33Y70/10
CPCA61L27/24A61L27/20A61L27/56A61K47/42C08J9/28C08J9/40B33Y70/10C08J2389/00C08J2305/08C08J2201/0484C08L89/00C08L5/08
Inventor 杨树林朱帅金明杰周潮辉王小琼王子勋
Owner NANJING UNIV OF SCI & TECH
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