67results about How to "Improve implantation rate" patented technology

A method is provided for the improvement of implantation rates and / or pregnancy rates in a female mammal, comprising administering to a female mammal in whom pregnancy is desired an effective amount of (a) a nitric oxide synthase substrate, a nitric oxide donor, or both, optionally in combination with (b) a progestin, and, (c) optionally, in further combination with an estrogen. A method is also provided for fertility control for a female mammal, comprising administering to a female mammal in whom pregnancy is not desired and at risk for becoming pregnant an effective amount of nitric oxide synthase inhibitor in combination with an antiprogestin. Pharmaceutical compositions are also provided.

A method is provided for the improvement of implantation rates and / or pregnancy rates in a female mammal, comprising administering to a female mammal in whom pregnancy is desired an effective amount of (a) a nitric oxide synthase substrate, a nitric oxide donor, or both, optionally in combination with (b) a progestin, and, (c) optionally, in further combination with an estrogen. A method is also provided for fertility control for a female mammal, comprising administering to a female mammal in whom pregnancy is not desired and at risk for becoming pregnant an effective amount of nitric oxide synthase inhibitor in combination with an antiprogestin. Pharmaceutical compositions are also provided.

The invention provides a protocol leading to improved embryo implantation rates and / or decreased miscarriage rates in which hCG, or a bio-analogue, is administered during the follicular phase.

The invention discloses a pregnant sow special-purposed bioactive feed additive, a feed premix, and a feed batch. The feed additive is prepared through the steps that: (1) a Chinese herbal medicine is prepared into Chinese herbal medicine powder, wherein the powder comprises the raw materials of, by weight: 15-30 parts of Mongolian milkvetch root, 6-15 parts of ligusticum, 10-25 parts of pilose asiabell root, 5-20 parts of largehead atractylodes rhizome, 5-20 parts of licorice root, 15-30 parts of immature tangerine fruit and dried tangerine peel, 10-40 parts of malt, 6-15 parts of Chinese pulsatilla, 8-20 parts of prepared rehmannia rhizome, and 16-40 parts of Szechwan lovage rhizome; (2) 15-20 parts by weight of a bioactive probiotics fermentation broth is uniformly mixed with 15 parts by weight of silicon dioxide; the mixture is subjected to low-temperature drying, such that a bioactive probiotics solid powder preparation is prepared; (3) 90% by weight of the Chinese herbal medicine powder obtained in the step (1) is uniformly mixed with 10% of the bioactive probiotics solid powder preparation obtained in the step (2), such that the feed additive is obtained. When the additive is used for feeding pregnant sows, healthy developments of fetuses in the mothers can be promoted. Also, pregnant sow miscarriage, stillbirths, mummified pig, postnatal hunger strike, mastitis, endometritis, and the like can be prevented. With the feed additive, piglet vitality and birth weight can be improved.

The present invention relates to the use of an oxytocin receptor antagonist in females undergoing embryo transfer as part of an assisted reproductive technology. In particular, methods are provided for increasing ongoing implantation rate, increasing ongoing pregnancy rate, increasing clinical pregnancy rate, and / or increasing live birth rate in a female subject undergoing embryo transfer. Specifically, the antagonists are released in the luteal phase when the endometrium is receptive for embryo implantation and / or when the embryo has reached the blastocyst-stage.

The invention relates to a manufacturing method for animal feed, in particular to a manufacturing method for a special animal feed exclusively used in fox, mink and raccoon dog. The method has the advantages that: raw materials of corn and soybean meal required in the formula are bulked by a dry method or wet method and ground; the ground material is mixed with meat and bone meal and imported fishmeal, and then bulked again (by the dry method or wet method); the second bulking is completely different from other bulking methods for an annular space discharging mode is adopted in the discharging in front of a bulking machine in the second bulking and the bulking temperature is 100-130 DEG C, which not only realizes the aim of sterilization and curing, but also avoids breaking the nutritional factors of the materials due to over-high temperature; the product produced by the process has high bulking degree, good water binding capacity, good palatability and thorough sterilization. The premix materials are added and mixed twice to avoid reducing the effect caused by heating.

A method is provided to functionally select cells with enhanced characteristics relevant to cell engraftment, including both spontaneous migration and directional migration towards specific chemo-attractants. The cells are preferably undifferentiated cells, such as mesenchymal stem cells. The method involves entrapping or encapsulating the cells in a biomaterial barrier, optionally inducing cell migration, and selecting cells that migrated through the barrier. The cells selected by this method have better migratory activities and enhanced in vivo engraftment to injured tissues when they are supplemented systemically.

The invention relates to umbilical cord mesenchymal stem cells (MSCs) and a culture method and application thereof, and belongs to the technical field of biological medicine. The culture method includes MSCs culture steps and phenotypic examination. The MSCs culture steps include primary culture and sub-culturing; during primary culture, Huatong gum tissue of the umbilical cord is obtained and cultured in a serum-free culture medium under hypoxic conditions; during sub-culturing, P1 primary cells are collected, a single cell suspension is prepared, cell precipitates are obtained after centrifuging, the serum-free culture medium is added into the cell precipitates, culturing is conducted under hypoxic conditions until next generations come out, continuous culturing is performed to obtain P2-P3 generations, ligustrazine hydrochloride and Shenmai injections are added in each sub-culturing stage, when the cells grow to a predetermined fusion degree, digestion is performed and the cells arecollected until the P6 generations come out. During phenotypic examination, the collected cells are subjected to phenotypic examination for use. An MSCs preparation obtained through culturing in themethod reduces the easy-to-aggregation characteristics of the stem cells, the occurrence of cell adhesion, erythrocyte rouleaux formation and cell mass embolism after intravenous infusion into the human body is thus avoided, and the stem cell (MSCs) technology can be better applied to clinical practice.

The invention provides a protocol leading to improved embryo implantation rates and / or decreased miscarriage rates in which hCG or LH, or a bio-analogue thereof, is administered during the follicular phase.

The present invention relates to a biodegradable suture-type cell delivery system, a preparation method thereof and a biodegradable suture comprising the same. The biodegradable suture-type cell delivery system has improved cell compatibility as a result of depositing a hydrophilic functional group-containing compound on the surface of a biodegradable suture yarn made of a biodegradable polymer, a portion of which has partially a bulky structure beneficial for the proliferation of cells or living tissue, so as to hydrophilically modify the surface, and then bonding a cell compatible material to the surface, and also functions as a cell culture scaffold, because the bulky structure is beneficial for the proliferation of cells. Accordingly, the biodegradable suture-type cell delivery system can improve the rate of engraftment of stem cells in vivo.

The invention discloses a cocooning and cocoon harvesting composite cocooning tool. The composite cocooning tool comprises a cocooning frame, a cocoon harvesting frame and supporting feet, the cocooning frame comprises transverse frame strips, longitudinal frame strips and cocoon falling columns, the cocoon harvesting frame is composed of transverse cocooning sheet strips and longitudinal cocooning sheet strips, and the supporting feet are arranged on the same side, without the cocoon falling columns, of the cocooning frame; and the cocooning frame is supported on the ground through the supporting feet, the overall air permeability of the device is improved, the cocoon falling columns on the transverse frame strips and the longitudinal frame strips are transversely and longitudinally arranged at intervals, an independent cocooning space is provided for silkworms, the nest entering rate is increased, the cross sections of the cocoon falling columns are designed to be non-circular, silkhanging is more convenient, and the cocooning rate is increased. The cocooning and cocoon harvesting cocooning tool can regularly provide an ideal and independent space for cocooning of silkworms, canimprove the silk hanging rate in the initial stage of silk and the implantation rate of silkworm cocoons, improves the spinning rate of the silkworm cocoons and the integrity of the silkworm cocoons,and can conduct cocoon harvesting and cocoon harvesting in a light, efficient and pollution-free mode.

The invention provides application of decidua NK cells and decidua NK cell exosomes from cell subsets in preparing drugs and auxiliary therapeutic agents for treating diseases related to infertility.Experiments prove that the exosomes treat endometrium growth disorders by promoting endometrial thickness increasing, improving endometrial cell activity, reducing endometrial cell damage, promoting VEGF expression, maintaining stem features of endometrium matrix cells and stimulating proliferation, so that the pregnancy success rate of endometrium damage model mice is increased to 50-70% from 20%; and the exosomes treat diseases related to maternal-fetal immunologic tolerance disorders by playing a role of immunologic tolerance, reducing a spontaneous abortion rate and improving a help T lymphocyte level. Besides, the exosomes can effectively increase the developmental rate of zygotes fertilized no matter in vitro or in vivo in a multiple mode, meanwhile, an in-vitro fertilization rate, an implantation rate of implantation and a birth rate are also increased, and positive assisting effects on infertility treatment are achieved.

The invention relates to feed set meals for pigs in different physiological stages, and discloses a systematic nutrition scheme for sows to increase the number of market commercial pigs each year, and the benefits of pig farms are improved accordingly. The systematic nutrition scheme comprises a sow fecundity N+ series product, a piglet higher survival rate 7030 scheme, a pig growing and fattening more-profit making V+ series product and matched feeding patterns. According to the sow fecundity N+ series product, healthy reproduction of boars can be guaranteed by providing enough maintenance, growth, reproduction and immunization nutrition. According to the piglet higher survival rate 7030 scheme, the nutritional requirements of pig stocks in each production stage are met by taking a 'comprehensive nutrients + high and balance nutritional levels + high-quality nutrient sources ' scheme into account, the digestive absorption capacity and the intestinal immunity of the pig stocks are improved by guaranteeing the gastrointestinal tract health, and the self immunity of the pig stocks is reinforced by guaranteeing production nutrition and paying close attention to immune nutrition and stress nutrition. The pig growing and fattening more-profit making V+ series product is prepared according to different nutritional requirements of the pigs in different growth and development stages, and the obtained commercial pigs grow fast, low in feed ration and good in meat quality.

The invention discloses broussonetia papyrifera antibiotic-free silage for pregnant black pigs. The broussonetia papyrifera antibiotic-free silage for pregnant black pigs is prepared from the following raw materials in parts by weight: 20-50 parts of fresh broussonetia papyrifera leaves, 10-15 parts of cassava dregs, 10-15 parts of soya bean meal, 2-5 parts of corn flour or rice bran or millet bran, 1-5 parts of passion fruit peels, 1-5 parts of crop leftovers, 0.5-2 parts of an enzyme preparation, 3-7 parts of sugars, 0.1-1 part of activated carbon powder, 0.01-0.1 part of microelements and 2-5 parts of probiotics. The invention further provides a preparation method of the broussonetia papyrifera antibiotic-free silage for the pregnant black pigs. By the fermented broussonetia papyriferaantibiotic-free silage, coarse fibers, hydrolyzed crude protein and total flavonoids are degraded, the content of polypeptide and the content of free amino acid are increased, the broussonetia papyrifera antibiotic-free silage is sour and fragrant, is easily absorbed by the pregnant pigs, and is good in palatability, by the silage, the function of the digestive system of the pregnant pigs can be improved, the implantation rate of fertilized eggs can be increased obviously, the litter size can also be increased obviously, the milk yield is increased, the immunity and disease resistance of the pregnant pigs are improved, health of suckling pigs is promoted, and growth of the suckling pigs is accelerated.

The invention provides a stem cell preparation for treating aplastic anemia and a preparation method thereof. The defects in the prior art are overcome, the occurrence rate of the graft versus host disease is decreased, more mesenchyme stem cells with the high immunosuppression function are obtained within a short period of time, the clinical requirements are met, a new thought is provided for the transplantation effect of stem cells, and important practical application value is brought into play. The invention further provides the preparation method of the stem cell preparation for treating aplastic anemia. According to the method, the mesenchymal stem cells are induced and cultivated through a special method, the immunosuppression capacity of the mesenchymal stem cells can be effectively improved and enhanced, and the mesenchymal stem cells can be further prepared into the stem cell preparation for treating aplastic anemia.

The invention discloses a traditional Chinese medicine composition for treating luteal phase defect type infertility and menstrual disorder. The traditional Chinese medicine composition disclosed by the invention is prepared from the following components in parts by weight: 1-6 parts of fried radix paeoniae alba, 1-9 parts of common yam rhizome, 1-6 parts of dogwood, 0.5-3 parts of vinegar baked bupleurum root, 1-9 parts of the seed of Chinese dodder, 1.5-9 parts of sliced cornu cervi, 1.5-9 parts of amethyst and 1-6 parts of radix salviae miltiorrhizae. According to the compound composition provided by the invention, by means of syndrome differentiation and treatment, the formula is obtained through a lot of experimental screening under the guidance of theories of traditional Chinese medicines; experimental results show that the traditional Chinese medicine composition is capable of improving the structure and form of ovary mitochondria endoplasmic reticulum, improving structures of endometrial glands and mesenchyme, adjusting ovary secreted active substances E2 and P, reducing endometrium estrogen receptors and progestrone receptors and increasing expression of integrins alpha v and beta 3; therefore, the endometrium receptivity can be increased; the embryo implantation action is promoted; the traditional Chinese medicine composition has the good effect for treating infertility; and furthermore, the traditional Chinese medicine composition is free from toxic and side effect, and safe and reliable to use.

The invention discloses a method for constructing an immunodeficiency mouse transplant model of human stem cells. The method comprises the following steps of: performing intraperitoneal injection on a receptor mouse (NOD / SCID) by adopting an N-acetylcysteine (NAC) antioxidant before transplanting, supplying drinking water containing NAC to the receptor mouse, continuously supplying the drinking water containing the NAC to the receptor mouse after the human stem cells are transplanted, and thus obtaining the immunodeficiency mouse transplant model of the human stem cells. The optimal model of NOD / SCID mouse transplant is successful established; and by the method, no matter in caudal vein or medullary cavity transplant, the implantation rate of the human stem cells is effectively improved, and the mouse is low in death rate and good in status in the transplant process and can survive for a long term. The method is simple, novel, easy to implement and low in wound to the mouse; and the NAC antioxidant used by the method is low in price.

The invention relates to an embryo transplantation tube which comprises an outer tube, a heat insulation tube and an embryo tube. The heat insulation tube is of an open or closed structural form. The heat insulation tube comprises a heating layer and a heat insulation layer. The outer tube is connected in the heat insulation tube. The outer tube comprises a straight part and a bent part. The bent part and the straight part are integrally formed in one time, one end of the straight part is provided with a fixed bolt, the other end of the straight part is provided with a cervix opening fixing part, and the cervix opening fixing part is movably connected to the straight part of the outer tube, the bent part is marked with a graduated scale, and the peripheral surface of the bent part is sleeved with a sealing cap. The embryo transplantation tube has the advantages that the embryo transplantation tube is low in cost, clinicians and laboratory workers operate the embryo transplantation tube conveniently, the transplanting efficiency is improved, the surgical operation time is shortened, the embryo transplantation tube has a fixing effect and conforms to the human physiological anatomy structure, it is guaranteed that an embryo is in a homoiothermal state before implantation, the embryo implantation rate and pregnancy rate can be improved, and the embryo transplantation tube can be further clinically popularized and applied later.

The invention relates to the field of functional foods and medicine materials, in particular to nanometer fungus dietary fibers and use thereof. The nanometer fungus dietary fibers disclosed by the invention are made from raw materials namely edible and medicinal funguses, and the nanometer fungus dietary fibers of which the granules are 200-300nm in particle diameter are made through technological processes of removing impurities, performing soaking in polished glutinous rice vinegar, performing rinsing, performing vacuum drying, performing superfine crushing, performing nanocrystallization crushing, performing sieving, performing freeze drying and the like. Through test, the nanometer fungus dietary fibers disclosed by the invention can significantly increase the implantation rate and the proliferation rate of probiotics; when the nanometer fungus dietary fibers are combined with the probiotics for preparing functional foods or medicines, the addition quantity of active probiotics can be reduced to a great extent, the cost and the price of products can be reduced, and the quality stability and the action effects of the products can be guaranteed.

The invention discloses a menstruation-regulating pregnancy-promoting pill. The menstruation-regulating pregnancy-promoting pill mainly comprises, by weight, 1-3% of pangolin scales, 1-3% of beautiful sweetgum fruit, 0.5-1.5% of fructus aurantii, 0.5-1.5% of bighead atractylodes rhizome, 8-12% of prepared rehmannia root, 13-17% of salvia miltiorrhiza, 0.5-1.5% of costusroot, 8-12% of cinnamon, 1-3% of fructus amomi, 3-7% of rhizoma cyperi, 8-12% of ligusticum wallichii, 8-12% of white peony root, 15-25% of Chinese angelica, 8-12% of himalayan teasel root and 0.5-1.5% of leonurus japonicus, and the weight percent sum of all the main materials is 100%. The menstruation-regulating pregnancy-promoting pill is capable of promoting ovulation, regulating menstruation, invigorating the kidney, strengthening Yang, regulating incretion and banishing stain and has remarkable curative effects on irregular menstruation, dysmenorrheal and amenorrhea of women.

The invention discloses a method for constructing a mixed sowing grassland by reseeding alfalfa in a slightly degraded leymus chinensis meadow, and relates to a method for constructing an artificial grassland in a degraded leymus chinensis meadow. The invention aims to improve the community structure of the leymus chinensis meadow and improve the yield and quality of forage grass. The method comprises the following steps that 1, in early July, the slightly degraded leymus chinensis meadow is cut, stubbles of 3-5 cm are reserved, and light raking operation is conducted; 2, after light raking operation, alfalfa seeds are sown, the sowing rate is 10 kg / hectare, and after sowing, a compacting machine is used for slight compacting; 3, in the current year of alfalfa sowing, the grassland is forbidden to be cut, the grassland is cut twice every year from the next year, the cutting time is selected at the initial flowering stage of alfalfa each time, and after the first cutting, the grassland is fertilized. According to the method, the yield of high-quality forage grass can be increased by 1.5-2 tons / hectare per hectare, the price of the forage grass is 1000 yuan / ton, the income can be increased by at least 1500 yuan / hectare every year, and the economic benefit in the later period is very considerable. The invention belongs to the field of degraded grassland recovery.

The invention discloses a rice seedling transplanting tube. The rice seedling transplanting tube having an opened mud discharging paw is adopted, a seedling insert channel is arranged nearby the mud discharging paw, the mud discharging paw is used to dig a hole in the field in advance, and a seedling is inserted into soil in a pneumatic manner, so the tube has the advantages of very simple operation, no foiling to hands, no need of repeated waist bending for seedling transplantation, and good social benefit.

The invention provides a traditional Chinese medicine composition for repairing endometrium and assisting reproduction. The traditional Chinese medicine composition comprises two parts, an external medicine and an internal medicine, wherein the external medicine is composed of processed monkshood, processed frankincense, myrrh, cinnamon, peach kernel, flos caryophylli, sinapis alba, aquilaria wood, and morinda officinalis with a specific proportion, the internal medicine is composed of Radix Rehmanniae Preparata, red sage root, Semen Cuscutae, aerial parts of epimedium, morinda officinalis, roasted tortoiseshell, Poria cocos, fried radix paeoniae rubra, Chinese angelica, and spatholobus stem. The traditional Chinese medicine composition has the advantages of convenient source of raw material, simple processing method, and wide application prospect.

The invention provides a protocol leading to improved embryo implantation rates and / or decreased miscarriage rates in which hCG, or a bio-analogue, is administered during the follicular phase.

The invention discloses a method for constructing an immunodeficiency mouse transplant model of human stem cells. The method comprises the following steps of: performing intraperitoneal injection on a receptor mouse (NOD / SCID) by adopting an N-acetylcysteine (NAC) antioxidant before transplanting, supplying drinking water containing NAC to the receptor mouse, continuously supplying the drinking water containing the NAC to the receptor mouse after the human stem cells are transplanted, and thus obtaining the immunodeficiency mouse transplant model of the human stem cells. The optimal model of NOD / SCID mouse transplant is successful established; and by the method, no matter in caudal vein or medullary cavity transplant, the implantation rate of the human stem cells is effectively improved, and the mouse is low in death rate and good in status in the transplant process and can survive for a long term. The method is simple, novel, easy to implement and low in wound to the mouse; and the NAC antioxidant used by the method is low in price.

The invention discloses a 3D printing collagen biological sponge scaffold and a preparation method thereof. The method comprises the steps that firstly, an acid solution containing chitosan and an acid solution containing collagen are mixed to be uniform to prepare 3D printing bio-ink, then the bio-ink is added into a printing gun head, and printing forming is conducted in a low-temperature environment according to design parameters and a model; and freeze-drying treatment is conducted on the freeze-formed product, the freeze-dried product is soaked in a solution containing procyanidine for cross-linking immobilization, and finally freeze-drying is performed after washing to obtain the recombinant human collagen biological sponge scaffold. By means of a low-temperature deposition 3D printing technology, all-environment temperature control can be achieved, and the forming stability is improved. The biological sponge scaffold has proper pores, forming controllability, good physical and chemical properties and biocompatibility, does not contain other impurities, and is low in manufacturing cost.

The invention belongs to the technical field of human assisted reproduction and relates to biopsy culture solution and a preparation method thereof. The biopsy culture solution is prepared by dissolving sucrose in concentration of 0.05-0.3mol / L into salt solution containing antibiotics and an indicator. By dissolution of sucrose in low molar ion into buffer salt solution without calcium and magnesium ions, embryo activity and plasma membrane integrality can be protected gently, embryo cell contraction is realized, adhesion to a biopsy needle is avoided, DNA integrality and activity can be improved after embryo biopsy, biopsy activity is protected, and accordingly biopsy sample detection effects are guaranteed, and the embryo implantation rate is increased. In addition, biopsy sample detection and assisted reproduction technology safety can be strongly guaranteed.

The invention provides a protocol leading to improved embryo implantation rates and / or decreased miscarriage rates in which hCG or LH, or a bio-analogue thereof, is administered during the follicular phase.