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Biopsy culture solution and preparation method thereof

A culture solution and biopsy technology, applied in biochemical equipment and methods, culture process, tissue culture, etc., can solve the problems of inability to effectively maintain the vitality and integrity of biopsy embryos, and the preservation of biopsy samples is not perfect, so as to improve the vitality and Effects of DNA integrity, enhancement of implantation rate, and activity protection

Pending Publication Date: 2018-11-02
瑞柏生物(中国)股份有限公司
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

At present, the routine biopsy culture medium used clinically is a protein solution buffered only with HEPES or MOPS saline solution, which cannot effectively maintain the viability and integrity of biopsy embryos, especially for biopsy samples.

Method used

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Examples

Experimental program
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Embodiment Construction

[0021] The present invention will be further described below in combination with specific implementation methods.

[0022] The biopsy culture solution of the present invention is prepared by dissolving sucrose at a concentration of 0.05-0.3 mol / L in a saline solution containing antibiotics and indicators. The antibiotic is gentamicin with a concentration of 5-25 μg / mL. The indicator is phenol red with a concentration of 4.5-10.0 μg / mL. The salt solution is a compound solution using HEPES or MOPS as a buffer, based on sodium and potassium ions, and using glucose, sodium pyruvate, sodium lactate, and albumin as energy substances; it contains: sodium chloride 98.11-102.00mmol / L, potassium chloride 4.55-4.70mmol / L, sodium bicarbonate 3.90-4.10mmol / L, HEPES or MOPS19.99-22.00mmol / L, glucose 2.68-2.88mmol / L, sodium pyruvate 0.31-0.33mmol / L L, sodium lactate 21.11-21.66mmol / L, potassium dihydrogen phosphate 0.36-0.39mmol / L; the albumin is recombinant human serum albumin, which is ...

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Abstract

The invention belongs to the technical field of human assisted reproduction and relates to biopsy culture solution and a preparation method thereof. The biopsy culture solution is prepared by dissolving sucrose in concentration of 0.05-0.3mol / L into salt solution containing antibiotics and an indicator. By dissolution of sucrose in low molar ion into buffer salt solution without calcium and magnesium ions, embryo activity and plasma membrane integrality can be protected gently, embryo cell contraction is realized, adhesion to a biopsy needle is avoided, DNA integrality and activity can be improved after embryo biopsy, biopsy activity is protected, and accordingly biopsy sample detection effects are guaranteed, and the embryo implantation rate is increased. In addition, biopsy sample detection and assisted reproduction technology safety can be strongly guaranteed.

Description

technical field [0001] The invention relates to a biopsy culture solution and a preparation method thereof, belonging to the technical field of human assisted reproduction. Background technique [0002] In human assisted reproductive technology, biopsy culture medium is mainly used in embryo biopsy in human assisted reproductive technology. At present, the routine biopsy culture medium used clinically is a protein solution buffered only with HEPES or MOPS saline solution, which cannot effectively maintain the viability and integrity of biopsy embryos, especially for the preservation of biopsy samples. Contents of the invention [0003] The purpose of the present invention is to provide a biopsy culture solution with various quality control standards, which can not only effectively preserve the vitality of biopsy embryos, improve the integrity of embryo DNA, but also provide a strong guarantee for the safety of assisted reproductive technology. [0004] In order to solve t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073
CPCC12N5/0603C12N2500/12C12N2500/30C12N2500/34
Inventor 冯怀亮
Owner 瑞柏生物(中国)股份有限公司
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