116 results about "Biopsy sample" patented technology

The invention provides a traditional Chinese medicine meridian prescription system based on multi-omics integration analysis of extracellular vesicles, and the system comprises a data collection module which is used for collecting biopsy sample information of different groups of individuals based on related experiments and literatures of traditional Chinese medicine meridian prescription treatment; the data processing module is used for obtaining and preprocessing extracellular vesicle data and intestinal flora data based on biopsy sample information; the multi-omics integration module is used for obtaining multi-omics integration data; the discovery and verification module is used for obtaining the potential menstrual prescription drug effect evaluation biomarker and performing experimental verification on the potential menstrual prescription drug effect evaluation biomarker to obtain a verification result; and the system biology application module is used for obtaining a regulatory mechanism of the extracellular vesicles in a biological system based on construction of a gene-protein-metabolite interaction network. The invention can comprehensively reveal the pharmacodynamic mechanism of the traditional Chinese medicine menstrual prescription, and provides strong technical support and application prospect for the modern, scientific and internationalized development of traditional Chinese medicines.

Systems and methods for determining a blood tumor mutational burden (bTMB) for a test subject are provided in which there is obtained, from a panel-enriched sequencing reaction, a plurality of nucleic acid sequences. The plurality of nucleic acid sequences comprises a corresponding sequence for each cell-free DNA fragment in a plurality of cell-free DNA fragments obtained from a liquid biopsy sample from the test subject. Each respective cell-free DNA fragment in the plurality of cell-free DNA fragments corresponds to a respective probe sequence in a plurality of probe sequences used to enrich cell-free DNA fragments in the liquid biopsy sample in the panel-enriched sequencing reaction. There is determined, using the panel-enriched sequencing reaction, that a circulating tumor fraction (ctFE) is above a threshold ctFE value. Responsive to this determination, the bTMB is calculated for the test subject from the panel-enriched sequencing reaction and reported.

A method and apparatus for preparing tissue of interest in a patient for possible excision by surgery. In one embodiment, the method comprises the steps of: removing a biopsy sample from the tissue of interest; placing a magnetic marker at the biopsy site; performing a pathology analysis of the biopsy sample; and if the pathology analysis indicates that the tissue of interest should be removed, locating the tissue for surgery using a magnetic detection probe. In one embodiment, the marker comprises magnetic nanoparticles in a bioabsorbable matrix. A system for preparing tissue of interest in a patient for possible excision by surgery. In one embodiment, the system includes a magnetic marker and magnetic detection probe system.

A core biopsy system includes a core biopsy needle device having a curvable core biopsy needle which is positionable within and movable with respect to a coaxial needle guide. Systems and methods for obtaining biopsy samples from multiple locations with a single insertion of a needle of the biopsy needle device include controlling rotation and displacement of the core biopsy needle. Additional features and methods of the invention include tracking and planning of the obtaining of biopsy samples.

Systems, methods, and devices include a biopsy tissue extraction device for creating, extracting and / or collecting a biopsy tissue sample. The biopsy tissue extraction device includes a housing with an extraction opening at a first distal end and an actuation handle at a second distal end. Extraction blade(s) extend out the extraction opening responsive to an engagement of the actuation handle. The extraction blade(s) move in a linear and / or circular motion, caused by a shaft connecting the extraction blade to the actuation handle, to cut the biopsy sample from a target tissue angle. The extraction blade(s) have a non-perpendicular or rotational approach angle with the target tissue area, such that the blade(s) fully sever the biopsy sample with a single motion.

A biopsy instrument (1) comprising a base member (10) which extends from a proximal end (10a) to a distal end (10b) along a central geometrical axis (A), wherein at least a distal end portion (10b′) of the base member (10) is shaped as an elongated hollow tube (10), the distal end (10b) being intended to be at least partly inserted into a tissue (50) from which a biopsy is to be obtained, wherein the hollow tube (10) is provided with a distally facing circular cutting edge (11) defining a mouth (10c) of the distal end (10b) of the hollow tube (10), wherein the hollow tube (10) has, at a distal portion (10b′) of the hollow tube (10), a hollow elongated tubular sample acquiring portion (10b′) having a smooth interior surface (12). The disclosure also relates to a kit of parts and a method of acquiring a biopsy.

Disclosed are methods and systems that make use of light scattering techniques, such as the use of a multiphoton laser-scanning microscope, to quantify the scattering directionality of second harmonic generation (SHG) from biopsy samples of cancer in order to predict efficacy of neoadjuvant chemotherapy.

The invention relates to the technical field of intelligent diagnosis of medical images, and discloses an intelligent diagnosis and classification method for biopsy pathological images. The method comprises the following steps: acquiring digital pathological image data of a biopsy sample, and extracting a morphological feature region in the image as a basic analysis unit; and constructing a multi-scale feature fusion network, inputting the basic analysis unit into the first feature extraction layer to obtain a primary feature map, and performing spatial attention weighting through the secondary feature extraction layer to generate a fusion feature vector. And establishing a dynamic classification threshold pool, dividing feature subspaces according to the dimension distribution of the fusion feature vector, and distributing independent classification boundary parameters for each subspace. And loading a pre-trained pathological knowledge map, carrying out similarity matching on the fusion feature vector and node features in the map, and screening out knowledge nodes of which the matching degrees exceed a preset threshold value as an auxiliary diagnosis basis. And generating an initial classification label based on an output result of the dynamic classification threshold pool and an auxiliary diagnosis basis, and outputting a final diagnosis classification result.

The utility model discloses a mammary gland biopsy sample storage device which comprises a storage box, the inner side of the box body is connected with a placing disc in a sliding mode, the bottom of the inner side of the box body is provided with a buffering mechanism facilitating shock absorption of the placing disc, and the top of the placing disc is provided with a plurality of sets of placing grooves facilitating placing of storage containers. According to the biopsy sample storage device, the multiple sets of biopsy samples can be stored conveniently through the arrangement of the containing disc and the multiple sets of containing grooves, buffering and damping protection can be provided for the placed biopsy samples through the arrangement of the buffering mechanism, the biopsy samples can be clamped and fixed through the arrangement of the clamping mechanisms, and the biopsy samples can be stored conveniently. According to the biopsy sample storage device, a storage container can be conveniently clamped and fixed, so that through the arrangement, the biopsy sample storage container can be conveniently clamped and fixed, the placement stability of the storage container is guaranteed, meanwhile, a better buffering protection effect can be provided for the biopsy sample storage container, and the stability of cells is guaranteed.

The invention relates to a method and system for detecting cells of a first cell type (1) in a biopsy sample in which a plurality of cells of different cell types (2, 3) are present. The invention further relates to a device for carrying out a magnetic field measurement on cells of a biopsy sample that are labeled with magnetic nanoparticles. The method comprises the following method steps: carrying out tissue dissociation of the biopsy sample in order to singulate the cells present in the biopsy sample; supplying magnetic nanoparticles (11) to the singulated cells in order to selectively label the cells of the first cell type (1); removing the excess magnetic nanoparticles (11); introducing the cells into a microchannel (5), the microchannel (5) having a cross section which allows only one labeled cell (1) to pass through at a time; carrying out a magnetic field measurement in the microchannel (5) by means of a quantum magnetic field sensor (6) based on the F-center principle; and sorting out the labeled cells (1) after they have passed through the microchannel (5).

The application discloses a gastric cancer immunotherapy efficacy prediction biopsy sample MHC-II expression kit and belongs to the field of kit preparation, and particularly relates to a preparation method of an antigen repair solution, which is prepared by mixing citric acid, sodium citrate, an additive and pure water, and the additive comprises a glucose amido polyether derivative and a propyl gallate / calcium ion complex. The gastric cancer immunotherapy efficacy prediction biopsy sample MHC-II expression kit is composed of the antigen repair solution; the kit comprises the following components in parts by mass: 20-30 parts of a dewaxing solution, 10-20 parts of the antigen repair solution, 2-10 parts of sheep serum, 10-20 parts of an enhancing solution, 15-25 parts of an antibody, 5-15 parts of a staining solution and 10-20 parts of a mounting agent. The gastric cancer immunotherapy efficacy prediction biopsy sample MHC-II expression kit provided by the application has high sensitivity and high specificity.

The invention relates to a method for preparing a sample for microscopic examination comprising the steps of using a chitosan-based porous material having interconnected pores with sizes between 5 μm and 700 μm and a total porosity (volumetric fraction) between 40% and 90%, adding at least one biopsy sample containing tissue and / or eukaryotic and / or prokaryotic cells and / or eukaryotic cells containing viral particles to the chitosan-based porous material; and adding (C1-C6)-alkyl alcohol or mixtures thereof.

The invention discloses an immunostaining reagent for MHC-II expression detection of a gastric cancer biopsy sample, and belongs to the field of bioengineering. The invention establishes a preparation method of an immunohistochemical staining section. The preparation method specifically comprises the following steps: step 1, early-stage treatment: baking a tissue section, dewaxing, rehydrating, subsequently adding an antigen solution for repairing, adding an endogenous enzyme inhibitor for incubation, adding a sealing agent for sealing and the like; step 2, antibody incubation: adding a first antibody and a second antibody for incubation; and step 3, post-treatment: dyeing, re-dyeing, bluing, sealing and the like. A mixed buffer solution is used as an endogenous enzyme inhibitor, so that the background staining intensity is effectively reduced, and the staining sensitivity and specificity are improved; meanwhile, a staining result interpretation standard is established, it is proved that MHC-II expression is remarkably related to pathological remission of the gastric cancer patient, and an evaluation method is provided for pathological remission of the gastric cancer patient.

Processes and materials to detect neoplasms from a biopsy are described. Processes and materials to build a sequencing library are described. Processes and material to perform targeted sequencing are described. Processes and materials to mitigate confounding sources are described. Cell-free nucleic acids can be sequenced and the sequencing result can be utilized to detect sequences derived from a neoplasm.

The invention discloses a methylation marker for differential diagnosis of breast malignant tumors and breast benign tumors and application of the methylation marker. The research finds that the methylation of the biopsy sample AJAP1 can be used as a potential marker for differential diagnosis of benign breast tumors, malignant breast tumors, different subtypes or different stages of malignant breast tumors. The invention has important scientific significance and clinical application value for identifying benign breast tumors, malignant breast tumors, different subtypes or different stages of malignant breast tumors and guiding and formulating a reasonable clinical treatment scheme.

A method capable of determining a probability of a subject having one or multiple cancer tissues of origin is disclosed. The method includes inputting the expression profile of a miRNA set obtained from a biopsy sample such as serum sample of the subject into a classifier which is based on a machine learning model such as a support vector machine model. In certain embodiments, the method can be used to simultaneously determine the probability of the subject having each of multiple different cancer types, which can enhance the prediction accuracy.

The present application relates to the technical field of medical image intelligent diagnosis, and discloses a biopsy pathological image intelligent diagnosis classification method. The method collects digital pathological image data of biopsy samples, extracts morphological feature regions in the image as a basic analysis unit. A multi-scale feature fusion network is constructed, the basic analysis unit is input into a first feature extraction layer to obtain a primary feature atlas, spatial attention weighting is performed through a secondary feature extraction layer, and a fusion feature vector is generated. A dynamic classification threshold pool is established, a feature subspace is divided according to the dimension distribution of the fusion feature vector, and independent classification boundary parameters are allocated to each subspace. A pre-trained pathological knowledge graph is loaded, the fusion feature vector is matched with the node features in the graph in terms of similarity, and knowledge nodes with a matching degree exceeding a preset threshold are selected as auxiliary diagnosis basis. An initial classification label is generated based on the output result of the dynamic classification threshold pool and the auxiliary diagnosis basis, and a final diagnosis classification result is output.

A method is disclosed comprising providing a biological sample on a swab, directing a spray of charged droplets onto a surface of the swab in order to generate a plurality of analyte ions, and analysing the analyte ions.

Provided is a miniature biopsy device for use in a patient. The miniature biopsy device is configured to be operated remotely. store and collect a biopsy sample within the miniature device. Also provided is the related system configured to carry out such an operation. and the related methods for use in biopsies.

The application discloses a use of MHC-II expression in prediction of curative effect of gastric cancer immunotherapy and belongs to the field of bioengineering. Specifically, the application comprises the following steps: detecting MHC-II expression in a gastric cancer biopsy sample; baking, dewaxing and gradient alcohol treating the gastric cancer biopsy sample slice; carrying out antigen repairing and blocking treatment; then adding diluted MHC Class II antibody for incubation, and then adding immunopotentiator and enhanced enzyme-labeled antibody for incubation; finally, completing immunostaining through DAB color development, staining liquid staining and counter blue treatment, mounting and observing. The MHC-II expression signal is clear and distinguishable, the staining effect is good, and through negative and positive expression interpretation and pathological remission evaluation, it is confirmed that the MHC-II expression is significantly related to pathological remission of a gastric cancer patient and plays an important role in prediction of curative effect of gastric cancer immunotherapy.

The invention relates to a bending control device, a biopsy device, biopsy device moving equipment and a biopsy system, and belongs to the field of interventional biopsy sampling technical equipment. The device comprises a first mounting box, a first transmission mechanism and a bending control part, wherein the tail part of the biopsy accessory is inserted into the first mounting box along a first direction; the first transmission mechanism is arranged in the first mounting box, and the first transmission mechanism is used for outputting reciprocating motion in the first direction; the bending control part extends in the length direction of the biopsy accessory, the two ends of the bending control part are fixedly connected with the first transmission mechanism and the head end of the biopsy accessory respectively, and when the first transmission mechanism drives the tail end of the bending control part to slide relative to the tail of the biopsy accessory and enables the bending control part to be tightened, the bending control part is driven to rotate relative to the tail of the biopsy accessory. The head end of the bending control piece is bent towards the tail end of the bending control piece. According to the invention, the effect of independently controlling the bending of the head end of the biopsy accessory is realized.

This invention discloses a bone marrow cell paraffin block, its preparation method, and its application, belonging to the field of pathological detection technology. The preparation method includes the following steps: Red blood cell lysis: Bone marrow aspirate is taken, and a precipitate containing cells and sediment is separated. A red blood cell lysis agent is added to lyse the red blood cells, and a lysed precipitate containing unlysed cells and sediment is separated. Formation: The lysed precipitate is resuspended in formalin solution for fixation, the supernatant is removed, and the precipitate is resuspended in agar solution. The agar is cooled and solidified, and then fixed again in neutral formalin. Dehydration: The solidified agar block is dehydrated. Embedding: The dehydrated agar block is embedded in paraffin to form a paraffin block. The cell paraffin block prepared by this method can supplement the diagnosis when bone marrow smears or bone marrow biopsy samples are not ideally prepared.

The present invention relates to a combination punch biopsy tool comprising both cutting means cutting a biopsy or sample from a cutaneous or mucosal surface, and a gripping part for catching and safe-handling of the biopsy or sample. In particular, the present invention relates to a punch biopsy tool for providing a skin or mucous membrane or tissue sample (15) comprising a cutting edge (1) and gripping means (2, 2a, 2b) for gripping a biopsy sample, wherein the cutting edge (1) is positioned at a first end of the punch biopsy tool, while the gripping means (2, 2a, 2b) are positioned at a second end of the punch biopsy tool, both the cutting edge (1) and the gripping means (2, 2a, 2b) are secured to a central body.

Systems, methods, and devices, for melanoma pathology using one or more neural networks (NNs). The techniques described herein may include receiving molecular data corresponding to a patient biopsy sample; molecular data; encoding molecular output data according to a preselected encoding scheme; receiving image data corresponding to the patient biopsy sample; applying the image data to an image analysis NN; concatenate the encoded molecular data to the image data at an intermediate layer of the image analysis NN; and producing, based on the encoded molecular information and the image information concatenated at the intermediate layer, a prognosis output from the image analysis NN. Many other features and examples are described herein.

A biopsy sample secondary sampling device comprises an air bag, and a one-way air inlet valve is arranged on the rear portion of the air bag. An air blowing connector is arranged on the front portion of the air bag and connected with the air bag in a pluggable or threaded mode. An air blowing channel is arranged in the air blowing connector, and an airtight ring is arranged in the air blowing channel. The utility model solves the problem that the secondary sampling of the biopsy sample is difficult in the prior art, realizes the complete secondary sampling of the biopsy sample, avoids the rupture of the sample, and is beneficial to the subsequent segmentation and pathological detection. Particularly, the device is simple in structure, low in manufacturing cost, capable of being combined and disassembled to facilitate cleaning and sterilization, capable of being reused and high in medical popularization value.

An ultrasonic endoscope assembly (110) has an endoscope (111) defining one or more lumens and a needle (114) that can be pushed forward from a lumen to collect a biopsy sample. An ultrasonic subassembly (120) is attached to the endoscope (111) and includes a communication cable (126) including a set of conductors having a distal end and an ultrasonic imaging head (122) connected to the distal end of the cable. Further included is an imaging head movement subassembly (140) including a conduit (148) releasably connected to the endoscope. The conduit houses a tension member (144) that is releasably connected to the imaging head (122). Thus, the imaging head movement subassembly (140) can be released from the cable and imaging head after use for disposal.

A biological structure identification system includes an optical imaging system configured to illuminate a liquid biopsy sample for a subject. The liquid biopsy sample has one or more biological structures that are labeled with one or more fluorophores associated with a fluorescence assay for a cancer allowing detection of emitted electromagnetic radiation from the liquid biopsy sample as image data. The system also includes a processing system configured to identify a cancer stage for the subject by determining a predetermined cancer stage profile from a set of predetermined cancer stage profiles to which the subject profile is most similar.

The invention relates to the technical field of medical instruments, and discloses a hematology department bone marrow biopsy puncture sampling device which comprises a handheld seat. The trocar is fixedly mounted on the handheld seat, and a rectangular opening is formed in the surface of the bottom of the trocar; the needle body is inserted into the trocar, a rectangular groove matched with the rectangular opening is formed in the surface of the needle body, and a precisely machined sharp cutting edge is designed on the edge of the rectangular groove; according to the bone marrow biopsy puncture sampling device for the hematology department, the sealed cavity is formed through dislocation after sampling is completed, tissue is wrapped in the groove and prevented from making contact with path tissue chippings on the surface of the trocar, sample pollution is reduced from the source, the problem that in a traditional device, path tissue is mixed into biopsy samples is solved, and the pollution condition is reduced; meanwhile, the isolation sleeve is clamped in the annular clamping groove through the elastic buckle piece and is taken out along with the needle body, so that impurities in the external environment are prevented from polluting a sample, and tissue fragments are prevented from falling into the external environment.

A method includes inserting an introducer needle into a tissue to be biopsied. A biopsy sample is obtained through the introducer needle from the tissue and the tissue proximate a tip of the introducer needle is ablated by an ablation antenna without removing the introducer needle. When removing the introducer needle from the tissue, the method may include coagulating blood in or cauterizing an insertion path in the tissue with the ablation antenna as the introducer needle is removed from the tissue. The insertion path is formed by the insertion of the introducer needle into the tissue.

The invention relates to a biopsy sample flattening, fixing and embedding box and a use method thereof, belongs to the technical field of medical instruments, solves the technical problem of standard storage, fixing and embedding of biopsy samples, and adopts the technical scheme that a box body is detachably clamped in the embedding box and comprises a hollow inner cavity, a through hole is formed in the head end of the upper top surface of the box body and communicated with the inner cavity, and the through hole is communicated with the inner cavity; a guide plate is arranged above the through hole in a covering manner; the flattening fixing plate is detachably installed on the bottom face of the inner cavity, the sliding plate is placed above the flattening fixing plate, and the sliding plate slides in the length direction of the inner cavity; a biopsy sample to be flattened is placed in the box body through the through hole, one end of the biopsy sample to be flattened is pasted on the first pasting fixing layer at the head end of the flattening fixing plate, and the other end of the biopsy sample to be flattened is pasted on the second pasting fixing layer at the head end of the sliding plate. The device is simple in structure, convenient to operate, time-saving, labor-saving and standard, ensures that the mucous membrane surface, the intrinsic layer and the mucous membrane muscle layer are fully exposed when a biopsy sample is fixed, meets the requirements of pathological diagnosis, and avoids missed diagnosis and misdiagnosis.