76 results about "Biopsy sample" patented technology

The invention relates to the technical field of intelligent diagnosis of medical images, and discloses an intelligent diagnosis and classification method for biopsy pathological images. The method comprises the following steps: acquiring digital pathological image data of a biopsy sample, and extracting a morphological feature region in the image as a basic analysis unit; and constructing a multi-scale feature fusion network, inputting the basic analysis unit into the first feature extraction layer to obtain a primary feature map, and performing spatial attention weighting through the secondary feature extraction layer to generate a fusion feature vector. And establishing a dynamic classification threshold pool, dividing feature subspaces according to the dimension distribution of the fusion feature vector, and distributing independent classification boundary parameters for each subspace. And loading a pre-trained pathological knowledge map, carrying out similarity matching on the fusion feature vector and node features in the map, and screening out knowledge nodes of which the matching degrees exceed a preset threshold value as an auxiliary diagnosis basis. And generating an initial classification label based on an output result of the dynamic classification threshold pool and an auxiliary diagnosis basis, and outputting a final diagnosis classification result.

The invention relates to a method and system for detecting cells of a first cell type (1) in a biopsy sample in which a plurality of cells of different cell types (2, 3) are present. The invention further relates to a device for carrying out a magnetic field measurement on cells of a biopsy sample that are labeled with magnetic nanoparticles. The method comprises the following method steps: carrying out tissue dissociation of the biopsy sample in order to singulate the cells present in the biopsy sample; supplying magnetic nanoparticles (11) to the singulated cells in order to selectively label the cells of the first cell type (1); removing the excess magnetic nanoparticles (11); introducing the cells into a microchannel (5), the microchannel (5) having a cross section which allows only one labeled cell (1) to pass through at a time; carrying out a magnetic field measurement in the microchannel (5) by means of a quantum magnetic field sensor (6) based on the F-center principle; and sorting out the labeled cells (1) after they have passed through the microchannel (5).

The application discloses a gastric cancer immunotherapy efficacy prediction biopsy sample MHC-II expression kit and belongs to the field of kit preparation, and particularly relates to a preparation method of an antigen repair solution, which is prepared by mixing citric acid, sodium citrate, an additive and pure water, and the additive comprises a glucose amido polyether derivative and a propyl gallate / calcium ion complex. The gastric cancer immunotherapy efficacy prediction biopsy sample MHC-II expression kit is composed of the antigen repair solution; the kit comprises the following components in parts by mass: 20-30 parts of a dewaxing solution, 10-20 parts of the antigen repair solution, 2-10 parts of sheep serum, 10-20 parts of an enhancing solution, 15-25 parts of an antibody, 5-15 parts of a staining solution and 10-20 parts of a mounting agent. The gastric cancer immunotherapy efficacy prediction biopsy sample MHC-II expression kit provided by the application has high sensitivity and high specificity.

A method capable of determining a probability of a subject having one or multiple cancer tissues of origin is disclosed. The method includes inputting the expression profile of a miRNA set obtained from a biopsy sample such as serum sample of the subject into a classifier which is based on a machine learning model such as a support vector machine model. In certain embodiments, the method can be used to simultaneously determine the probability of the subject having each of multiple different cancer types, which can enhance the prediction accuracy.

The present application relates to the technical field of medical image intelligent diagnosis, and discloses a biopsy pathological image intelligent diagnosis classification method. The method collects digital pathological image data of biopsy samples, extracts morphological feature regions in the image as a basic analysis unit. A multi-scale feature fusion network is constructed, the basic analysis unit is input into a first feature extraction layer to obtain a primary feature atlas, spatial attention weighting is performed through a secondary feature extraction layer, and a fusion feature vector is generated. A dynamic classification threshold pool is established, a feature subspace is divided according to the dimension distribution of the fusion feature vector, and independent classification boundary parameters are allocated to each subspace. A pre-trained pathological knowledge graph is loaded, the fusion feature vector is matched with the node features in the graph in terms of similarity, and knowledge nodes with a matching degree exceeding a preset threshold are selected as auxiliary diagnosis basis. An initial classification label is generated based on the output result of the dynamic classification threshold pool and the auxiliary diagnosis basis, and a final diagnosis classification result is output.

A method is disclosed comprising providing a biological sample on a swab, directing a spray of charged droplets onto a surface of the swab in order to generate a plurality of analyte ions, and analysing the analyte ions.

Provided is a miniature biopsy device for use in a patient. The miniature biopsy device is configured to be operated remotely. store and collect a biopsy sample within the miniature device. Also provided is the related system configured to carry out such an operation. and the related methods for use in biopsies.

The application discloses a use of MHC-II expression in prediction of curative effect of gastric cancer immunotherapy and belongs to the field of bioengineering. Specifically, the application comprises the following steps: detecting MHC-II expression in a gastric cancer biopsy sample; baking, dewaxing and gradient alcohol treating the gastric cancer biopsy sample slice; carrying out antigen repairing and blocking treatment; then adding diluted MHC Class II antibody for incubation, and then adding immunopotentiator and enhanced enzyme-labeled antibody for incubation; finally, completing immunostaining through DAB color development, staining liquid staining and counter blue treatment, mounting and observing. The MHC-II expression signal is clear and distinguishable, the staining effect is good, and through negative and positive expression interpretation and pathological remission evaluation, it is confirmed that the MHC-II expression is significantly related to pathological remission of a gastric cancer patient and plays an important role in prediction of curative effect of gastric cancer immunotherapy.

This invention discloses a bone marrow cell paraffin block, its preparation method, and its application, belonging to the field of pathological detection technology. The preparation method includes the following steps: Red blood cell lysis: Bone marrow aspirate is taken, and a precipitate containing cells and sediment is separated. A red blood cell lysis agent is added to lyse the red blood cells, and a lysed precipitate containing unlysed cells and sediment is separated. Formation: The lysed precipitate is resuspended in formalin solution for fixation, the supernatant is removed, and the precipitate is resuspended in agar solution. The agar is cooled and solidified, and then fixed again in neutral formalin. Dehydration: The solidified agar block is dehydrated. Embedding: The dehydrated agar block is embedded in paraffin to form a paraffin block. The cell paraffin block prepared by this method can supplement the diagnosis when bone marrow smears or bone marrow biopsy samples are not ideally prepared.

The present invention relates to a combination punch biopsy tool comprising both cutting means cutting a biopsy or sample from a cutaneous or mucosal surface, and a gripping part for catching and safe-handling of the biopsy or sample. In particular, the present invention relates to a punch biopsy tool for providing a skin or mucous membrane or tissue sample (15) comprising a cutting edge (1) and gripping means (2, 2a, 2b) for gripping a biopsy sample, wherein the cutting edge (1) is positioned at a first end of the punch biopsy tool, while the gripping means (2, 2a, 2b) are positioned at a second end of the punch biopsy tool, both the cutting edge (1) and the gripping means (2, 2a, 2b) are secured to a central body.

Systems, methods, and devices, for melanoma pathology using one or more neural networks (NNs). The techniques described herein may include receiving molecular data corresponding to a patient biopsy sample; molecular data; encoding molecular output data according to a preselected encoding scheme; receiving image data corresponding to the patient biopsy sample; applying the image data to an image analysis NN; concatenate the encoded molecular data to the image data at an intermediate layer of the image analysis NN; and producing, based on the encoded molecular information and the image information concatenated at the intermediate layer, a prognosis output from the image analysis NN. Many other features and examples are described herein.

An ultrasonic endoscope assembly (110) has an endoscope (111) defining one or more lumens and a needle (114) that can be pushed forward from a lumen to collect a biopsy sample. An ultrasonic subassembly (120) is attached to the endoscope (111) and includes a communication cable (126) including a set of conductors having a distal end and an ultrasonic imaging head (122) connected to the distal end of the cable. Further included is an imaging head movement subassembly (140) including a conduit (148) releasably connected to the endoscope. The conduit houses a tension member (144) that is releasably connected to the imaging head (122). Thus, the imaging head movement subassembly (140) can be released from the cable and imaging head after use for disposal.

The invention relates to the technical field of medical instruments, and discloses a hematology department bone marrow biopsy puncture sampling device which comprises a handheld seat. The trocar is fixedly mounted on the handheld seat, and a rectangular opening is formed in the surface of the bottom of the trocar; the needle body is inserted into the trocar, a rectangular groove matched with the rectangular opening is formed in the surface of the needle body, and a precisely machined sharp cutting edge is designed on the edge of the rectangular groove; according to the bone marrow biopsy puncture sampling device for the hematology department, the sealed cavity is formed through dislocation after sampling is completed, tissue is wrapped in the groove and prevented from making contact with path tissue chippings on the surface of the trocar, sample pollution is reduced from the source, the problem that in a traditional device, path tissue is mixed into biopsy samples is solved, and the pollution condition is reduced; meanwhile, the isolation sleeve is clamped in the annular clamping groove through the elastic buckle piece and is taken out along with the needle body, so that impurities in the external environment are prevented from polluting a sample, and tissue fragments are prevented from falling into the external environment.

The invention relates to a biopsy sample flattening, fixing and embedding box and a use method thereof, belongs to the technical field of medical instruments, solves the technical problem of standard storage, fixing and embedding of biopsy samples, and adopts the technical scheme that a box body is detachably clamped in the embedding box and comprises a hollow inner cavity, a through hole is formed in the head end of the upper top surface of the box body and communicated with the inner cavity, and the through hole is communicated with the inner cavity; a guide plate is arranged above the through hole in a covering manner; the flattening fixing plate is detachably installed on the bottom face of the inner cavity, the sliding plate is placed above the flattening fixing plate, and the sliding plate slides in the length direction of the inner cavity; a biopsy sample to be flattened is placed in the box body through the through hole, one end of the biopsy sample to be flattened is pasted on the first pasting fixing layer at the head end of the flattening fixing plate, and the other end of the biopsy sample to be flattened is pasted on the second pasting fixing layer at the head end of the sliding plate. The device is simple in structure, convenient to operate, time-saving, labor-saving and standard, ensures that the mucous membrane surface, the intrinsic layer and the mucous membrane muscle layer are fully exposed when a biopsy sample is fixed, meets the requirements of pathological diagnosis, and avoids missed diagnosis and misdiagnosis.

1. The name of the design product: the main body of biopsy needle. 2. The use of the design product: the whole is used for the tool of obtaining the biopsy sample or cell sample from the patient's body for the pathological examination of the pathologist, and the protected part is used for the main body of the biopsy needle. 3. The design points of the design product: the shape of the protected part. 4. The picture or photo that can best show the design points: perspective view 1. 5. Other circumstances that need to be explained: the dotted line in the figure is not the part to be protected.

The invention relates to the technical field of medical image processing, in particular to a lupus nephritis curative effect prediction system based on multi-staining kidney biopsy pathological images. The system comprises a feature extraction module used for extracting image features of staining biopsy images of patients, a similarity analysis module used for distinguishing reference patients from non-reference patients according to the image features, a data preparation module used for forming training samples, and a data processing module used for processing the training samples. And the prediction module is used for performing model training based on the training data and predicting the healing probability of the target patient. According to the scheme, the model can be helped to pay more attention to training data of biopsy samples which are similar to biopsy samples of a target patient and are effectively different from biopsy samples of other reference patients, the characteristic of high heterogeneity existing in lupus nephritis is matched, the information mining effect of the biopsy sample image of lupus nephritis is improved, and the accuracy of the biopsy sample image of lupus nephritis is improved. And a doctor is assisted to accurately complete evaluation of specific typing and severity of lupus nephritis of a patient.

The invention relates to the technical field of medical auxiliary instruments, in particular to a nail and breast surgery biopsy sample preservation device which comprises a protection shell, a plurality of L-shaped supporting plates are arranged on the inner wall of the protection shell, through holes are formed in the short edges of the L-shaped supporting plates, supporting columns are arranged in the through holes in a sliding fit mode, and push plates are fixedly connected to the ends, close to the inner wall of the protection shell, of the supporting columns. Springs sleeving the supporting columns are arranged on the sides, close to the supporting columns, of the push plates, and the other ends of the springs are fixedly connected with the L-shaped supporting plates; arc-shaped clamping blocks are arranged at the other ends of the supporting columns; rotating shafts corresponding to the pushing blocks in number are arranged on the inner wall of the protective shell and are rotationally connected with pushing assemblies located below the long edges of the L-shaped supporting plates and used for pushing the pushing plates. The invention aims to realize full-process protection of abnormal posture-automatic clamping-stable resetting through a mechanical linkage structure, so that the stability and safety of a sample tube in a transfer process are improved, and the preservation requirements of high integrity and low pollution risk of clinical samples in the department of thyroid and breast surgery are met.

A system for automated biopsy analysis is provided. The system includes a biopsy needle holder and delivery system configured to secure a biopsy needle, a sample transfer system configured to transfer a biopsy sample from the biopsy needle to a well of a multi-well plate, a liquid handling system configured to dispense and aspirate liquid to and from the well of the multi-well plate, an optical measurement system configured to measure a fluorescent signal from the well, and a control system configured to control operation of the biopsy needle holder and delivery system, the sample transfer system, the liquid handling system, and the optical measurement system. The system enables automated processing and analysis of biopsy samples, improving efficiency and reproducibility of metabolic rate determination and other tissue analysis.

The utility model relates to a biopsy sampling and positioning system. The biopsy sampling and positioning system comprises a sampling assembly and an auxiliary positioning assembly, wherein the sampling assembly comprises a sheath tube and an inner core, and a sampling structure is arranged at the front ends of the inner core and the sheath tube; the auxiliary positioning assembly comprises a positioning locking piece which is adjustably arranged on the sheathing canal in the length direction of the sheathing canal; the scale plate is provided with a first containing groove used for containing the sheathing canal and a second containing groove used for containing the positioning locking piece, the scale plate is further provided with scale marks arranged in the length direction of the first containing groove, and the second containing groove is communicated with the first containing groove and located at the starting end of the scale marks; and the optical navigation component comprises a reference frame arranged on the sheathing canal and an optical ball fixed on the reference frame. According to the scheme, the sampling length can be calibrated in advance, so that a sampling structure can quickly and accurately reach a focus part, the quick positioning of a sampling assembly is completed, accurate navigation can be performed, the visual operation of sampling is realized, and the sampling operation is more refined.

The present invention belongs to the technical field of immune cell culture, and specifically relates to a composition and kit for expanding tumor infiltrating lymphocytes, and an expansion method therefor. Provided is a composition for expanding tumor infiltrating lymphocytes for a trace amount of tumor tissue, i.e., a tumor biopsy sample, to perform the expansion of tumor infiltrating lymphocytes, which solves the problem that a large amount of a tumor tissue sample is required for tumor infiltrating lymphocytes, and is suitable for patients who cannot undergo surgical resection to obtain tumor tissue. In addition, by means of optimizing the combination and working concentration of antibody stimulants, a process flow for expanding tumor infiltrating lymphocytes on the basis of biopsy samples is established, such that the stemness of the expanded tumor infiltrating lymphocytes is maintained, and the killing ability is improved, thereby ensuring the anti-tumor efficacy after reinfusion into the body.

A computer-implemented method includes to determine a cell, tissue or a lesion representation in cell-free DNA comprises inputting, to a processor, cell-free DNA (cfDNA) genomic profiles from one or more fluid biopsy samples from a patient and one or more genomic profiles from one or more cells, tissues or lesions from the patient; constructing, by the processor, a plurality of synthetic fluid hypotheses (SFs); comparing, by the processor, each of the plurality of SFs to the cfDNA genomic profiles to determine goodness of fit, of each of the plurality of SFs; selecting, by the processor, a subset of the plurality of SFs, wherein each SF of the subset of SFs has a minimum distance in goodness of fit compared to the cfDNA genomic profile; and outputting, by the processor, based on the subset of SFs, a cell, tissue or a lesion representation in the cfDNA of the patient.

The present invention provides a method for determining whether a patient will respond to treatment with a myostatin pathway inhibitor, the method comprising: (a) determining a level of myostatin and / or activin type II receptor (ActRII) and / or follistatin in at least one muscle biopsy obtained from a treatment target muscle in a subject having or suspected of having muscle atrophy or a muscle wasting condition; and (b) determining a level of myostatin and / or follistatin in a systemic sample obtained from the patient, wherein if: (i) the level of myostatin in the systemic sample is higher than a threshold and / or if the level of follistatin in the sample is lower than a threshold; and (ii) the level of myostatin and / or ActRII receptor in the at least one biopsy sample is higher than a threshold level and / or if the level of follistatin in the at least one biopsy sample is lower than a threshold level, the patient will respond to treatment.

The application provides a traditional Chinese medicine classical prescription system for analyzing extracellular vesicles based on multi-omics integration, comprising: a data acquisition module for acquiring biopsy sample information of different groups of individuals based on related experiments and literature of traditional Chinese medicine classical prescription treatment; a data processing module for obtaining and preprocessing extracellular vesicle data and intestinal flora data based on the biopsy sample information; a multi-omics integration module for obtaining multi-omics integration data; a discovery and verification module for obtaining potential classical prescription efficacy evaluation biomarkers and experimentally verifying the potential classical prescription efficacy evaluation biomarkers to obtain verification results; and a systems biology application module for obtaining the regulation mechanism of extracellular vesicles in a biological system based on the construction of a gene-protein-metabolite interaction network. The application can comprehensively reveal the efficacy mechanism of traditional Chinese medicine classical prescriptions and provide strong technical support and application prospects for the modernization, scientification and internationalization development of traditional Chinese medicines.

Disclosed herein is a method for enhancing antitumor T cell responses in subjects. The method involves administering to the subject in need thereof a composition comprising a demethylating agent in an amount effective to demethylate STING proteins in the tumor cells. This method is particularly useful in subjects with deficient STING expression in the tumor cells. Therefore, also disclosed is a method for treating a tumor in a subject that involves detecting in a biopsy sample from the subject reduced STING expression, reduced cGAS expression, or a combination thereof; and then administering to the subject a demethylating agent in an amount effective to demethylate STING proteins in the tumor cells. The method can further involve administering to the subject a therapeutically effective amount of a STING agonist. The method can further involve administering to the subject tumor infiltrating lymphocytes (TILs), such as HLA-matched TILs.

The present invention concerns the field of cancer and methods for early detection of cancerous lesions. Specifically, the invention relates to a method for early detection of cancerous lesions through the analysis of microsatellite instability in the genome of an individual from a liquid biopsy.

The invention develops a prediction model based on pathomics characteristics of a conventional biopsy sample, and the prediction model is used for evaluating the recurrence risk of EGFR mutation LUAD patients receiving EGFR-TKI treatment. According to pathological omics data, five pathological omics features are found for the first time, and a patient scoring model pathomicScore is constructed based on the five features. The result shows that the pathomicScore model is in negative correlation with the progression-free lifetime of the disease. Besides, a comprehensive prediction model of the disease progression risk is developed by combining a pathomicScore model with a model based on clinical data, and the model has a good layering capability for the LUAD patient, so that a personalized treatment strategy can be guided. The method has an application prospect in the fields of risk assessment of EGFR targeted therapy and precise tumor.

Disclosed are methods for detecting and treating cancers (such as carcinoma, leukemia, lung cancer, colon cancer, central nervous system (CNS) cancer, melanoma, ovarian cancer, renal cancer, oral cancer, prostate cancer and / or breast cancer). The method may include receiving a sample from a patient (such as a sample from a biopsy, a sample from an extracellular vesicle, or a sample from a circulating tumor cell). The method may include determining whether a LY6C protein or a phosphorylated ANXA2 protein is detected in the sample. The method may include treating a patient for cancer by targeting the LY6C protein when the LY6C protein is detected (e.g., using photoimmunotherapy (PIT) or near infrared photoimmunotherapy (NIR-PIT)). or treating a patient for cancer by targeting an ANXA2 protein (e.g, using inhibitors, decoys, etc.) when the phosphorylated ANXA2 protein is detected.

The invention belongs to the technical field of immune cell culture, and particularly relates to a tumor infiltration lymphocyte amplification composition, a kit and an amplification method thereof. The invention provides the tumor infiltration lymphocyte amplification composition aiming at a trace amount of tumor tissue, namely a tumor puncture sample, the tumor infiltration lymphocyte amplification composition is used for amplifying the tumor infiltration lymphocyte, the problem that the tumor tissue sample size required by the tumor infiltration lymphocyte is large is solved, and the tumor infiltration lymphocyte amplification composition is suitable for a patient who cannot receive the tumor tissue obtained by surgical excision. Besides, by optimizing the antibody stimulant combination and the working concentration and establishing the technological process for amplifying the tumor infiltration lymphocytes based on the puncture sample, the dryness of the tumor infiltration lymphocytes obtained through amplification is maintained, the lethality is improved, and therefore the anti-tumor curative effect after the tumor infiltration lymphocytes are infused back into the body is guaranteed.

The present disclosure provides a fluidic device including a first inlet, an outlet, and a channel positioned between the first inlet and the outlet. The channel is in fluid communication with the first inlet and the outlet. The fluidic device further includes a second inlet positioned between the first inlet and the outlet. The second inlet is in fluid communication with the channel. The fluidic device further includes a pump in fluid communication with the second inlet. The pump is configured to provide a first volume of pulsatile flow to the channel. The first volume of pulsatile flow is greater than about 50 μL per pulse.