The invention discloses a production method of gecko wine. The production method comprises the following steps: (1) taking a live gecko, washing by adopting saline water, slightly hitting the head of the live gecko till death, removing the head, the feet, the scales and the internal organs, completely washing away the blood and slime, soaking by adopting sodium bicarbonate solution, fishing up, and washing till the gecko is clean; (2) soaking the gecko by adopting fishiness-removing water for 4-6 h, airing the water on the surface of the gecko, and carrying out sterilization; (3) sealing the sterilized gecko, placing the sealed gecko into a freezing chamber for freezing, taking out the gecko, removing the sealing device, and placing the gecko into a low-temperature drying machine for drying for 21-30 h; and (4) soaking the gecko by adopting soy sauce flavor type baijiu as base liquor for 2-24 months. According to the production method, the water inside the body of the gecko is removed through the low-temperature drying manner, so that the fishy smell volatilizes along with the sublimation of the water, and meanwhile, the effective components inside the body of the gecko can be maintained to the greatest extent, so that the integral quality of the product can be improved.