Microbial limit detection method for membrane used for multi-layer coextrusion infusion

Abstract

The invention relates to a microbial limit detection method for a membrane used for multi-layer coextrusion infusion. The method includes the steps of taking out the membrane used for multi-layer coextrusion infusion under the aseptic environment, flushing the outer layer face of the membrane used for multi-layer coextrusion infusion through a stroke-physiological saline solution at least three times, taking a membrane material with the area of 50 cm<2> in the middle, cutting the taken membrane material with the area of 50 cm<2> into pieces through a pair of aseptic scissors, placing the cut membrane material in a conical aseptic bottle, adding 100 ml of aseptic sodium chloride peptone buffer solutions with the pH value of 7.0, shaking the mixture for 1 minute, standing still for 10 minutes, preparing a test solution, taking 10 ml of the test solution and filtering the test solution through a membrane filter after a filter membrane of the membrane filter is wetted through 10 ml of the aseptic sodium chloride peptone buffer solutions with the pH value of 7.0, taking out the filter membrane, attaching the filter membrane to a culture medium with the bacterium face of the filter membrane facing upwards, taking 10 ml of the test solution, inoculating the test solution to 100 ml of soybean casein digestion meat juice, placing the culture medium where the filter membrane is attached and the soybean casein digestion meat juice where inoculation is conducted in an incubator, and observing the result. By means of the established microbial limit detection method, the quality standard of the membrane used for multi-layer coextrusion infusion is improved, and gaps in microbial limit detection are filled in.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a method for detecting the limit of microorganisms for a multilayer co-extrusion transfusion film. Background technique [0002] Multi-layer co-extrusion film for infusion is mainly used for infusion soft bag. Because infusion products are a variety with extremely high safety requirements, multi-layer co-extruded infusion films are used as packaging materials for the production of ready-to-use medicines without washing. (I), bag and YBB00102005 five-layer co-extrusion film for infusion (I), bag standards clearly specify the detection of bacterial endotoxin items, but do not control the microbial limit. Microbiological limit detection of multi-layer co-extrusion infusion film is beneficial to control the degree of biological contamination during product production. [0003] At present, the detection method of the microbial limit of the composite film ...

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a simple, easy-to-operate multi-layer co-extruded co-extrusion film for the control of the non-microbial limit inspection items of the existing multi-layer co-extruded transfusion film, and the traditional detection method is easy to cause secondary pollution. Microbial limit testing method for membranes used for extrusion transfusion

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