Mass detection method for dendrobium officinale medicine
A quality inspection method, the technology of Dendrobium candidum, applied in the field of medicine, can solve the problems of difficult determination of the content of the characteristic active ingredients of Dendrobium candidum, and difficulty in distinguishing whether it contains the characteristic ingredients of Dendrobium candidum, so as to achieve perfect quality standards, ensure safety, and good repeatability Effect
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Embodiment 1
[0027] Embodiment 1: Extraction method investigation
[0028] 1. Weigh 1g of Dendrobium officinale powder that has passed through a 50-mesh sieve, 4 parts in total, which are respectively marked as A, B, C, and D;
[0029] A. Ultrasonic extraction with 50ml ethyl acetate for 20min; B. Thermal extraction with 50ml 80% ethanol for 2h; C. Ultrasonic extraction with 40ml acetone for 30min; D. Thermal extraction with 60ml methanol for 1h.
[0030] Take 4ml of the extract and centrifuge for 10min at a speed of 10000r / min. Take the supernatant and dilute it by a certain number of times to measure the total bibenzyl content. The benzyl compound is extracted, preferably the ethanol thermal extraction method.
[0031] The total bibenzyl extraction rate of different extraction methods in table 1
[0032] Test No.
[0033] After the above A, B, C, and D extracts were evaporated to dryness, they were redissolved with methanol, and then detected with a Waters 2695-2996 Photodiod...
Embodiment 2
[0034] Embodiment 2: bibenzyl reference substance
[0035] By measuring the ultraviolet absorption spectrum of the reference substance and the extract, as shown in Table 2, it is determined that the vicinity of 280nm is a reasonable measurement interval for the total bibenzyl ultraviolet spectrophotometry.
[0036] Table 2 UV maximum absorption of different substances
[0037]
Embodiment 3
[0038] Embodiment 3: Ethanol hot bill of lading factor experiment
[0039] 3.1 Ethanol concentration
[0040] Weigh 0.5g of Dendrobium officinale powder that has passed through a 50-mesh sieve, add 25ml of ethanol aqueous solution of different concentrations, reflux for extraction for 2 hours, let cool, filter, accurately measure the filtrate and dilute it 50 times, measure the absorbance A at 280nm, and then from the regression equation Calculate the concentration C in, and then calculate the bibenzyl extraction rate, the relationship between the obtained ethanol concentration and the bibenzyl extraction rate is as follows figure 1 Shown: when the ethanol concentration is 50%, the extraction rate of bibenzyl is the highest, which is 8.87%.
[0041] 3.2 Extraction temperature
[0042] Weigh 0.5g dried stem powder of Dendrobium officinale through a 50-mesh sieve, add 25ml of 50% ethanol, extract for 2 hours at different temperatures, let cool, filter, accurately measure the f...
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