Mass detection method for dendrobium officinale medicine

A quality inspection method, the technology of Dendrobium candidum, applied in the field of medicine, can solve the problems of difficult determination of the content of the characteristic active ingredients of Dendrobium candidum, and difficulty in distinguishing whether it contains the characteristic ingredients of Dendrobium candidum, so as to achieve perfect quality standards, ensure safety, and good repeatability Effect

Active Publication Date: 2014-12-24
NINGBO FENGKANG BIOLOGICAL TECH
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Problems solved by technology

At present, the practice of identifying the raw materials of Dendrobium candidum by traits and identifying products with crude polysaccharides as the main index is not only difficult to distinguish whether it contains the charac

Method used

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  • Mass detection method for dendrobium officinale medicine
  • Mass detection method for dendrobium officinale medicine
  • Mass detection method for dendrobium officinale medicine

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0027] Example 1: Investigation of extraction method

[0028] 1. Weigh 1g of Dendrobium officinale powder that has passed a 50-mesh sieve, a total of 4 parts, and mark them as A, B, C, and D;

[0029] A, 50ml ethyl acetate ultrasonic extraction for 20min; B, 50ml 80% ethanol thermal extraction for 2h; C, 40ml acetone ultrasonic extraction for 30min; D, 60ml methanol thermal extraction for 1h.

[0030] Centrifuge 4ml of the extract for 10 minutes at a speed of 10000r / min. Dilute the supernatant by a certain multiple to determine the total bibenzyl content. The total bibenzyl extraction rate in each extraction method is shown in Table 1: These solvents can all The benzyl compounds are extracted, preferably by ethanol thermal extraction.

[0031] Table 1 Total bibenzyl extraction rate of different extraction methods

[0032] Test number

[0033] After the above extracts A, B, C, and D were evaporated to dryness, they were reconstituted with methanol, and then tested with Waters 2695-29...

Example Embodiment

[0034] Example 2: Bibenzyl reference substance

[0035] By measuring the ultraviolet absorption spectra of the reference substance and the extract, as shown in Table 2, it is determined that the vicinity of 280nm is a reasonable measurement interval of total bibenzyl ultraviolet spectrophotometry.

[0036] Table 2 UV maximum absorption of different substances

[0037]

Example Embodiment

[0038] Example 3: Ethanol hot bill of lading factor experiment

[0039] 3.1 Ethanol concentration

[0040] Weigh 0.5g of Dendrobium officinale powder that has passed through a 50-mesh sieve, add 25ml of ethanol aqueous solution of different concentrations, reflux for 2h, let cool, filter, accurately measure the filtrate and dilute 50 times, measure the absorbance A at 280nm, and then use the regression equation Calculate the concentration C in, and then calculate the bibenzyl extraction rate. The relationship between the ethanol concentration and the bibenzyl extraction rate is as follows figure 1 Shown: when the ethanol concentration is 50%, the extraction rate of bibenzyl is the highest, 8.87%.

[0041] 3.2 Extraction temperature

[0042] Weigh 0.5g dried stem powder of Dendrobium officinale that has passed a 50-mesh sieve, add 25ml of 50% ethanol, extract for 2h at different temperatures, let cool, filter, accurately measure the filtrate and dilute it 50 times, measure A at 280nm, ...

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Abstract

The invention discloses a mass detection method for dendrobium officinale medicine; total bibenzyl content in the medicine is detected by an ultraviolet spectroscopy; the mass detection method comprises the steps of: adopting bibenzyl compound as a reference substance, detecting total bibenzyl content by using an external standard method at 270-290nm. The method is simple, convenient, easy to implement and good in repeatability, can accurately reflect mass of the dendrobium officinale medicine, further completes quality standards of the dendrobium officinale medicine, and covers the shortage that mass evaluation of the dendrobium officinale is lack of the method for detecting fat-soluble characteristic components which are represented by bibenzyl; and therefore, the mass detection technology of the dendrobium officinale medicine is more scientific and reasonable. By adopting the detection method provided by the invention, mass of the dendrobium officinale medicine can be effectively controlled, and thereby safety, stability and effectiveness of clinical application of the dendrobium officinale medicine are ensured.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for detecting the quality of Dendrobium candidum medicinal materials. Background technique [0002] Dendrobium candidum is a perennial herbaceous plant of the family Dendrobium. Its main medicinal parts are fresh or dried stems. It is mainly distributed in Anhui, Zhejiang, Guangxi, Hunan, Yunnan, Guizhou and other places. It is a traditional precious Chinese medicinal material. Dendrobium officinale has the effect of benefiting the stomach and promoting body fluid, nourishing yin and clearing away heat. It is used for yin injury and fluid deficiency, dry mouth and polydipsia, insufficient stomach yin, retching due to lack of food, persistent deficiency heat after illness, hyperactivity of fire due to yin deficiency, fatigue and heat due to bone steaming, Obscurity of eyesight, muscle and bone flaccidity and weakness embolisms. "Shen Nong's Materia Medica" listed it as ...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 陈贤良
Owner NINGBO FENGKANG BIOLOGICAL TECH
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