Active Ganoderma lucidum polysaccharide peptide reference substance, and preparation method and application thereof

A technology of Ganoderma lucidum polysaccharide peptide and reference substance, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., and can solve the problems that cannot truly reflect the quality of Ganoderma lucidum products, cannot exclude the interference of excipients, and the content of polysaccharides in Ganoderma lucidum products is too high.

Active Publication Date: 2012-07-18
FUJIAN ZHENGYUAN JUNCAO INT COOP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, during the production process of Ganoderma lucidum extract, starch or other starch-based auxiliary materials are often added to the Ganoderma lucidum extract, so that the method cannot eliminate the in

Method used

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  • Active Ganoderma lucidum polysaccharide peptide reference substance, and preparation method and application thereof
  • Active Ganoderma lucidum polysaccharide peptide reference substance, and preparation method and application thereof
  • Active Ganoderma lucidum polysaccharide peptide reference substance, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] 1. An active ganoderma polysaccharide peptide (GL-PPS) reference substance, which has the following characteristics:

[0065] (1) Properties: light brown to brown powder;

[0066] (2) Properties: soluble in water, insoluble in ethanol, acetone, ether, etc., easy to be affected by moisture;

[0067] (3) Purity: over 99%;

[0068] (4) Peak molecular weight (MP) 1.06~1.2×10 4 ;

[0069] (5) Chemical structure: composed of rhamnose (Rha), mannose (Man) and glucose (Glc), containing 17 kinds of amino acids, and the glycosidic bond is β-configuration;

[0070]

[0071] Its main chain is composed of 1,3- and 1,4-glucose alternately connected, and there are branches at the 6-position of part of the main chain glucose, and the non-reducing ends are all composed of glucose, and the main chain is also composed of 1, 6 or 1 , the composition of 3-linked β-D-Glcp, with branches at the 2 or 3 position of the main chain glucose of part 1.6-;

[0072] (6) The percent molar rati...

Embodiment 2

[0075] A preparation method of active ganoderma lucidum polysaccharide peptide (GL-PPS) reference substance, its steps are as follows:

[0076] (1) Select the fruiting body of Ganoderma lucidum, cut it into thin slices (2-20mm) or crush it, pass through a 40-60 mesh screen, add 10-30 times the volume of deionized water, soak it at 90°C for 2 hours, 90-100 Extract at ℃ for 1.5-2.0 hours, stir and stir intermittently, centrifuge at 3000-4000rpm, remove the precipitate and take the extract, add water 5-10 times the weight of the atomic entity to the residue after centrifugation, repeat the extraction twice, and combine the extracted supernatant;

[0077] (2) Concentrate the supernatant to 5-10 times of the original feeding amount, centrifuge to remove impurities, leave the clear liquid, and dialyze (36MD) for 48 hours, concentrate half of the dialysate, and centrifuge again (10000-12000rpm) to remove impurities , keep the supernatant;

[0078] (3) Slowly add three times the volu...

Embodiment 3

[0092] Example 3. The method for detecting the content of ganoderma lucidum polysaccharide peptide products using GL-PPS as a reference substance:

[0093] 1. Detection condition test

[0094] 1.1 Selection of wavelength: scan on a spectrophotometer with 0.25% GL-PPS (900-450nm), the maximum absorption peak is at 761.50nm, and the absorption value is 0.509. Therefore, the detection absorption wavelength is selected as 760nm.

[0095] 1.2 Preparation of test solution

[0096] 1.2.1 Preparation of Ganoderma lucidum test solution: Accurately weigh 5 g of crushed Ganoderma lucidum fruiting body and place it in a 100 mL Erlenmeyer flask, add 20 times the volume of distilled water, ultrasonicate at 60°C for 15 minutes, cool and filter to obtain the filtrate ①. Add 10 times the volume of distilled water to the filter residue, sonicate at 60°C for 15 minutes, cool and filter to obtain the filtrate ②. Combine the filtrates from ① and ②, concentrate by evaporation, centrifuge at 4000...

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Abstract

The invention discloses an active Ganoderma lucidum polysaccharide peptide reference substance, and a preparation method and application of the reference substance, and provides an active Ganoderma lucidum polysaccharide peptide reference substance for measuring and evaluating the quality of Ganoderma lucidum and Ganoderma lucidum products. The reference substance can correct the errors in polysaccharide measurement generated using glucose as the reference substance after addition of starch as an adjuvant during the deep processing process of Ganoderma lucidum. The preparation method uses Ganoderma lucidum as a raw material and comprises the steps of soaking in water, boiling, collecting an extractive solution, concentrating, centrifuging, dialyzing, entrapping polysaccharide protein GL-PP with an active macromolecular site, further carrying out ethanol gradient separation of the GL-PP, centrifuging, and collecting precipitates; and separately carrying out column separation, and selecting active polysaccharide peptide GL-PPS as the reference substance for detecting polysaccharide peptides, wherein the reference substance can be used for accurate measurement of the content of trace active polysaccharide peptides in a UV (ultraviolet) instrument. The pure product of the reference substance disclosed by the invention can be used for detecting the quality of Ganoderma lucidum products, and can eliminate the interference of starch and other adjuvants in the Ganoderma lucidum products, so as to improve the quality standards of Ganoderma lucidum products.

Description

technical field [0001] The invention relates to a reference substance for ganoderma lucidum and ganoderma product quality determination, in particular to a reference substance for active ganoderma lucidum polysaccharide peptide (GL-PPS), a preparation method and application. Background technique [0002] The existing method for determining the quality of Ganoderma lucidum and Ganoderma lucidum products, according to the anthrone-sulfuric acid method stipulated in the Chinese Pharmacopoeia, is suitable for dextran as the main structure, and the relative molecular mass is 1×10 4 Determination of the above water-soluble crude polysaccharides. In this method, glucose or dextran is used as the reference substance, and the content of active ingredients of polysaccharide peptides with medicinal value is ignored in terms of anhydrous glucose or dextran. In particular, during the production process of Ganoderma lucidum extract, starch or other starch-based auxiliary materials are of...

Claims

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Application Information

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IPC IPC(8): C07K14/37C07K1/36C07K1/34C07K1/30C07K1/16G01N30/02G01N21/31
Inventor 王赛贞林占熺林冬梅林志彬林树钱吕松涛罗虹建林辉
Owner FUJIAN ZHENGYUAN JUNCAO INT COOP CO LTD
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