36results about How to "Reducing ROS levels" patented technology

A method and system used for treating proliferative disorders using carotenoids, carotenoid analogs, and / or carotenoid derivatives. The method and system may be used for chemoprevention and / or chemotherapy. The method and system may induce apoptosis in target cells, tissues, and / or organs. The analog, derivative, or intermediate may be administered to a cell, a group of cells, a tissue, an organ or a subject, such that at least a portion of the undesirable consequences of the proliferative disorder are thereby reduced.

The invention provides a nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin, essence lotion, a preparation method of the nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin, and an application of the essence lotion. The nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin comprises the symbiotic bacterium combined fermented products, decarboxy carnosine HCl, palmitoyl pentapeptide-4, tocopheiyl acetate, a pale butterflybush flower extract, resveratrol, a pomegranate fruit extract and a narcissus bulb extract, wherein symbiotic bacteria of the symbiotic bacterium combined fermented productscomprise combination of schizophyllum commune, thermus thermophilus, microzymes and lactobacilli, and fermentation raw materials of the symbiotic bacterium combined fermented products comprise combination of rice, coix lacryma-jobi ma-yuen seeds, ginseng, kelp and raspberries. Through synergistic compounding of effective active components of the symbiotic bacterium combined fermented products andthe like, the nursing composition containing symbiotic bacterium combined fermented products, for resisting ageing and repairing skin can condition and repair skin ageing resistant routes of oxidation resistance, saccharification resistance, blue light resistance, collagen synthesis promotion and the like, so that cell vitality is protected, and the skin has the capacity for resisting exogenous environmental factors.

The invention discloses TPP mitochondria target astaxanthin emulsion. The TPP mitochondria target astaxanthin emulsion comprises components of 0.5-1mg / mL of casein, 0.1-0.2mL / mL of medium chain triglyceride, 0.02-0.04mL / mL of dimethyl sulfoxide, 0.025-0.05mg / mL of triphenyl phosphonium bromide, 0.025-0.05mg / mL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride), 0.01-0.025mg / mL of N-hydroxysuccinimide, 0.05-0.1mg / mL of astaxanthin, and the balance water. During preparation of the emulsion, casein is used for entrapping astaxanthin, and the surface of the emulsion is subjected to triphenyl phosphonium bromide modification, so that the emulsion can be distributed in the mitochondria in a targeted manner. Nutrient entrapping is realized, besides, nutrients are in targeted positioning, the bioactivity is developed, and interfering and destroying of bioactivity by external environment are avoided.

The invention relates to novel compounds that are useful for modulating cellular ROS. The compounds are amide-derivatives of 2-hydroxy-2-methyl-4-(3,5,6-trimethyl-1,4-benzoquinon-2-yl)-butanoic acid. The compounds of the invention are formulated into pharmaceutical or cosmetic compositions. The invention further relates to methods wherein the compounds of the invention are used for treating or preventing diseases associated with increased ROS levels mitochondrial disorders and / or conditions associated with mitochondrial dysfunction, including adverse drug effects. The invention also relates to cosmetic methods for treating or delaying further aging of the skin and veterinary applications.

The present invention discloses an in vitro culture liquid (mPZM-3) and a culture method for a pig parthenogenetic activation embryo, and belongs to the field of in vitro animal embryo culture. The in vitro culture liquid (mPZM-3) comprises raw materials such as a mPZM-3 base culture liquid, calcium lactate pentahydrate and sodium pyruvate, wherein the concentration of the calcium lactate pentahydrate in the in vitro culture liquid is 0.606 g / L, the concentration of the sodium pyruvate is 0.022 g / L, the solvent used by the in vitro culture liquid is hydrogen-rich water, and the H2 content is 1.2-1.6 ppm. The culture method for the pig parthenogenetic activation embryo by using the in vitro culture liquid comprises: washing a pig parthenogenetic activation embryo by using an in vitro culture liquid containing 5 [mu]mol / L cytochalasin B, treating for 3-5 h in the in vitro culture liquid containing 5 [mu]mol / L cytochalasin B, washing with a cytochalasin B-free in vitro culture liquid, and culturing for 168 h in the cytochalasin B-free in vitro culture liquid until the embryo develops into a blastula. According to the present invention, with the in vitro culture liquid and the culture method, the energy and the development environment can be well provided for the ig parthenogenetic activation embryo so as to improve the culture quality and the culture effect of the embryo.

The invention discloses a new application of isopentene-based flavonoids compound IAA (isoangustone A) in a drug. The new application disclosed by the invention is as follows: pharmaceutically acceptable salt, ester, solvolyte, stereoisomers, tautomers, prodrugs and a mixture of the salt, the ester, the solvolyte, the stereoisomers, the tautomers and prodrugs are applied in the following aspects: 1) preparing an anti-inflammatory bowel disease drug; 2) preparing a drug for preventing and / or treating colorectal cancer; 3) preparing an eucaryon colorectal cancer cell apoptosis inducer; and 4) preparing an NF-kB (nuclear factor-kappa B) signal inhibitor. The compound IAA can obviously induce the colon cancer cell apoptosis, and inhibit the activity of colon cancer cell and inflammation signals, and also can obviously alleviate the colon inflammation induced by dextran sulfate.

The invention relates to an application of high voltage electric filed in fruitage, in particular to a method for treating tomato with an alternative electric field. The method of the invention solves such critical problems of the prior art as long time for treating tomato under a constant high voltage electrostatic field , complicated operation and big electricity leakage while a high voltage is loaded. The tomatoes are placed in a high voltage alternative electric field environment at a frequency of 40kHz, an electric field intensity of 200kV / m and treated for 2 hours at a time, which can delay the ripening process of fruitage such as reddening and softening. The invention has the effects of deferring the hardness of fruitage, the decrease of soluble sugar content in the fruitage and chlorophyll content in fruit peel, the rising of titratable acid and lycopene content, etc.

The invention relates to a folic acid targeting vector loading the single-substituted epigallocatechin gallate palmitate. The folic acid targeting vector comprises a zeolite imidazole skeleton, single-substituted epigallocatechin gallate palmitate embedded in the zeolite imidazole skeleton as well as folic acid-polyethyleneglycol modified on the zeolite imidazole skeleton by virtue of an amido bond. The invention also relates to a preparation method and application of the folic acid targeting vector loading the single-substituted epigallocatechin gallate palmitate. By adopting the folic acid targeting vector, the antioxidant effect of the single-substituted epigallocatechin gallate palmitate is improved, not only can the problem that the utilization rate of the single-substituted epigallocatechin gallate palmitate is low be solved, but also the toxic and side effect in treating the cancer can be reduced, and the treatment of a drug has targeting performance.

The invention discloses a method for constructing an immunodeficiency mouse transplant model of human stem cells. The method comprises the following steps of: performing intraperitoneal injection on a receptor mouse (NOD / SCID) by adopting an N-acetylcysteine (NAC) antioxidant before transplanting, supplying drinking water containing NAC to the receptor mouse, continuously supplying the drinking water containing the NAC to the receptor mouse after the human stem cells are transplanted, and thus obtaining the immunodeficiency mouse transplant model of the human stem cells. The optimal model of NOD / SCID mouse transplant is successful established; and by the method, no matter in caudal vein or medullary cavity transplant, the implantation rate of the human stem cells is effectively improved, and the mouse is low in death rate and good in status in the transplant process and can survive for a long term. The method is simple, novel, easy to implement and low in wound to the mouse; and the NAC antioxidant used by the method is low in price.

The invention belongs to the technical field of biology and discloses an anti-aging exosome-SOD (superoxide dismutase) nano preparation and a preparation method thereof. According to a strategy of combination of an extrusion method and saponin, SOD enzyme molecules are loaded into exosome, and an action effect and an action mechanism of the nano preparation in senescence resisting are systematically evaluated by taking the prepared SOD@EXO nano preparation as a nano drug and taking N2 wild type caenorhabditis elegans as a model organism. By loading of SOD in the exosome, catalytic activity of natural enzymes is kept, and remarkable advantages such as half-life period prolonging, immunogenicity reducing and membrane permeability improving are achieved as compared with performances of natural SOD. The nano preparation is high in drug-loading capacity and capable of realizing stable drug release, and the low-immunogenicity high-catalytic-activity exosome-superoxide dismutase nano preparation is constructed finally to prolong the half-life period of SOD molecules and improve anti-aging treatment effects remarkably.

The invention relates to the technical field of biological medicines, in particular to a targeting phase-change nano-drug system as well as a preparation method and application thereof. The targetingphase-change nano-drug system comprises a main body, liquid fluorocarbon is wrapped in the main body, the main body is loaded with a drug, and the main body is covalently connected with BMS-470539. The medicine system can solve the problems of toxic and side effects and weak targeting when immunosuppressive treatment is adopted for immune-related kidney diseases in the prior art. The targeting phase-change nano-drug system can be applied to preparation of related drugs for treating immune nephropathy.

The invention belongs to the technical field of tissue repair medicines, and particularly relates to application of a TGF-beta inhibitor in preparation of a medicine for treating diabetic foot. Aiming at the problems that cells are easy to apoptosis and the treatment effect of cell transplantation is not lasting when the cells are used for treating the diabetic foot, the invention provides the application of the TGF-beta inhibitor in preparing the medicine for treating the diabetic foot, and the TGF-beta inhibitor is proved to be capable of promoting the cells to maintain the mitochondrial membrane potential to be stable under the conditions of high glucose and low nutrition; and the intracellular active oxygen level is reduced, so that the cells maintain good proliferative activity and vascularization function. The function-enhanced endothelial cells obtained through induction and domestication culture of the TGF-beta inhibitor can survive for a long time in limbs of the ischemic diabetic foot of a mouse and form new vessels, and the treatment effect of endothelial cell transplantation on the ischemic diabetic foot is improved. According to the invention, the application range of the TGF-beta inhibitor is widened, a more effective method is provided for the cell therapy of diabetic foot, and the TGF-beta inhibitor has a good prospect.

The invention relates to a flavonoid derivative with a ROCK kinase inhibiting effect, belongs to the technical field of organic synthetic medicines, and solves the technical problems that the existingglaucoma treating medicines are poor in effect, especially effective therapeutic medicines for treating glaucoma with normal intraocular pressure lack, and medicines for treating diabetic retinopathy, such as conbercept and ranibizumab, can only be applied to the later stage of a disease, are ineffective for the early stage of the disease and destroy the protective effect of VEGF on a normal retinal tissue after long-term use. A technical scheme for solving the problems is achieved by providing a compound shown as a formula I. Experimental results show that the compound has the significant ROCK kinase inhibiting effect, can effectively reduce the intraocular pressure, protect retinal ganglion cells, reduce the retinal reactive oxygen level and inhibit retinal angiogenesis, and provides anovel medication choice for clinical treatment of the glaucoma and the diabetic retinopathy.

The invention discloses breast-milk infant formula goat milk powder capable of improving immunity and a preparation method thereof, and belongs to the technical field of food. The infant formula goatmilk powder mainly comprises the following raw materials: raw goat milk, high-oil desalted goat whey powder, goat whey protein powder, low-trans fatty acid compound vegetable oil, compound oligosaccharide, compound fruit and vegetable powder and various nutrient substances. The proportion of amino acids contained in the formula goat milk powder is closer to that of breast milk, the immunity of infants is improved through reasonable compatibility of various nutrient substances, and the burden of the digestive system of the infants is relieved; the invention further relates to the preparation method of the formula goat milk powder. The preparation method comprises the steps of raw goat milk acceptance and purification, pre-pasteurization, wet mixing, preheating, homogenization, sterilization, concentration, spray drying, dry mixing and the like, the operation is simple, and the process is easy to control; the formula goat milk powder is suitable for infants, and the preparation method issuitable for preparing the formula goat milk powder.

The invention discloses a method for constructing an immunodeficiency mouse transplant model of human stem cells. The method comprises the following steps of: performing intraperitoneal injection on a receptor mouse (NOD / SCID) by adopting an N-acetylcysteine (NAC) antioxidant before transplanting, supplying drinking water containing NAC to the receptor mouse, continuously supplying the drinking water containing the NAC to the receptor mouse after the human stem cells are transplanted, and thus obtaining the immunodeficiency mouse transplant model of the human stem cells. The optimal model of NOD / SCID mouse transplant is successful established; and by the method, no matter in caudal vein or medullary cavity transplant, the implantation rate of the human stem cells is effectively improved, and the mouse is low in death rate and good in status in the transplant process and can survive for a long term. The method is simple, novel, easy to implement and low in wound to the mouse; and the NAC antioxidant used by the method is low in price.

Methods of treating and preventing metabolic disease through inhibiting interleukin 11 (IL-11)-mediated signalling are disclosed, as well as agents for use in such methods.

The invention provides application of a natural product small molecular alpha-arbutin (Arb) to preparation of a medicine for preventing and controlling Parkinson's disease. The research result shows that the compound can be used for reducing the oxidative stress level in a nerve cell system and protecting neuronal cells. Furthermore, influences, caused by the Arb, on AMPK (Adenosine MonophosphateActivated Protein Kinase) and downstream protein p62 thereof are inspected through protein detection, and finds out that the Arb protects the neuronal cells from being injured by oxidative stress through inhibiting AMPK activation and p62 expression for the first time, so that the effect of preventing and controlling the Parkinson's disease is realized. The Arb is a novel autophagy activating agent derived from a natural product and has a good market application prospect.

The present disclosure relates, in general, to use of small diffusible thiols in the treatment of neurodegenerative diseases associated with glutamate excitotoxicity, protein aggregation and oxidative stress in the central nervous system, particularly in the brain.

The invention discloses an infant formula milk powder capable of optimizing amino acid patterns and improving immunity and a preparation method of the infant formula milk powder, and belongs to the technical field of food. The raw materials of the formula milk powder include raw milk, demineralized whey powder, concentrated whey powder, low trans-fatty acid compound vegetable oil, compound oligosaccharide, compound fruit and vegetable powder and various nutrients. Thus, the proportion of amino acid contained in the formula milk powder is closer to breast milk; and through the reasonable compatibility of the various nutrients, the immunity of infants can be improved, and the burdens of the digestive systems of the infants can be reduced. A preparation method of the formula milk powder is also disclosed. The preparation method includes raw milk acceptance and purification, pre-pasteurization, wet mixing, pre-heating, homogenization, sterilization, concentration, spray drying and dry mixing; and the preparation method is simple in operation and easy in process control. The formula milk powder is suitable for the infants to drink; and the involved preparation method is suitable for thepreparation of the formula milk powder.

The present invention provides an anti-aging and repairing skin care composition containing fermented products of symbiotic bacteria combination, essence milk and its preparation method and application. Palmitoyl pentapeptide‑4, tocopheryl acetate, Budisma japonica extract, resveratrol, pomegranate fruit extract and narcissus bulb extract; the symbiotic bacteria of the symbiotic bacteria combination fermentation product include Schizophyllum, A combination of Thermus thermus, saccharomyces and Lactobacillus; the fermented material of the symbiotic bacteria combination fermentation product includes a combination of rice, millet seeds, ginseng, macroalgae and raspberries. The anti-aging and repairing skin care composition can regulate and repair anti-aging pathways such as anti-oxidation, anti-glycation, anti-blue light, and promotion of collagen synthesis through the synergistic compounding of effective active ingredients such as symbiotic bacteria combination fermentation products, Thereby protecting the vitality of cells, so that the skin has the ability to resist exogenous environmental factors.

The invention relates to a preparation method of a pH-sensitive antioxidant nano-carrier. The preparation method comprises the following steps: (1) dissolving chitosan in distilled water to obtain a chitosan solution; (2) completely dissolving N-acetyl-L-histidine in dimethyl sulfoxide, and then adding N, N'-diimidazole; evaporating the solution to dryness after stirring, adding the chitosan solution and performing stirring, and performing freeze-drying after dialysis to obtain N-acetyl-L-histidine grafted chitosan; (3) taking coupling agents EDC and NHS, and adding N-acetylcysteine after uniform stirring to obtain carboxyl-activated N-acetylcysteine; and (4) mixing the N-acetyl-L-histidine grafted chitosan with the carboxyl-activated N-acetylcysteine, and then adding the mixture into a TEMED / HCI buffer solution; and reacting for 6-10h under magnetic stirring, performing dialysis by using a dialysis bag, and then carrying out freeze-drying treatment to obtain the pH-sensitive antioxidant nano-carrier. The antioxidant nano-carrier prepared by the invention can promote the survival of stem cells, and efficiently delivers and releases drugs in an acidic microenvironment in a targeted manner.

The invention relates to an application of high voltage electric filed in fruitage, in particular to a method for treating tomato with an alternative electric field. The method of the invention solves such critical problems of the prior art as long time for treating tomato under a constant high voltage electrostatic field , complicated operation and big electricity leakage while a high voltage isloaded. The tomatoes are placed in a high voltage alternative electric field environment at a frequency of 40kHz, an electric field intensity of 200kV / m and treated for 2 hours at a time, which can delay the ripening process of fruitage such as reddening and softening. The invention has the effects of deferring the hardness of fruitage, the decrease of soluble sugar content in the fruitage and chlorophyll content in fruit peel, the rising of titratable acid and lycopene content, etc.

The invention relates to flavonoid derivatives with ROCK kinase inhibitory effect, and belongs to the technical field of organic synthetic medicines. The technical problem to be solved by the present invention is that the existing glaucoma treatment drugs are not effective, especially the lack of effective treatment drugs for glaucoma with normal intraocular pressure; the drugs for the treatment of diabetic retinopathy such as conbocept and ranibizumab can only be used In the late stage of the disease, it is ineffective for the early stage of the disease, and long-term use will destroy the protective effect of VEGF on normal retinal tissue. The technical solution to solve the above problems is to provide the compound shown in formula I. The experimental results show that the compound of the present invention has a significant inhibitory effect on ROCK kinase, can effectively reduce intraocular pressure, protect optic ganglion cells, reduce retinal reactive oxygen levels and inhibit retinal angiogenesis, and provide a new method for clinical treatment of glaucoma and diabetic retinopathy. choice of medication.

The present disclosure relates, in general, to use of small diffusible thiols in the treatment of neurodegenerative diseases associated with glutamate excitotoxicity, protein aggregation and oxidative stress in the central nervous system, particularly in the brain.

The invention belongs to the technical field of irradiation and extraction of active ingredients of traditional Chinese medicines, and particularly discloses a method for preparing panax notoginseng polysaccharide with high anti-aging activity by utilizing irradiation, which comprises the following steps: spraying an irradiation sensitizer solution on panax notoginseng waste residues, and irradiating by electron beams until the dose is 30kGy-200kGy to obtain irradiated panax notoginseng residues; mixing the irradiated pseudo-ginseng dregs with water, and performing heating or ultrasonic extraction to obtain a pseudo-ginseng polysaccharide extracting solution; and concentrating the extracting solution under reduced pressure, adding acid to adjust the pH value to 4.5, adding ethanol into the filtrate, and freeze-drying the polysaccharide precipitate subjected to alcohol precipitation to obtain the panax notoginseng polysaccharide with the active polysaccharide content of more than 70%. The molecular weight of the panax notoginseng polysaccharide can be precisely regulated and controlled, compared with panax notoginseng polysaccharide processed without irradiation, the content of active polysaccharide is increased by 2-3 times or above, the processing capacity is large, the yield is high, energy consumption is low, the panax notoginseng polysaccharide is clean and free of pollution, large-scale industrial production can be achieved, and the antioxidant and anti-aging medicinal value of the panax notoginseng polysaccharide is further developed through the irradiation technology.

The invention discloses a medicament capable of delaying postharvest rot of a Manihot esculenta Crantz tuberous root, and a preparation method and application of the medicament. The medicament is an aqueous solution and comprises abscisic acid, melatonin and chitosan; and in each litre of medicament, the concentration of the abscisic acid is 400-600[mu]M / L, the concentration of the melatonin is 200-400[mu]M / L, and the concentration of the chitosan is 10-30mM / L. The level of active oxygen in the Manihot esculenta Crantz tuberous root dipped in the medicament disclosed by the invention is lowered, the browning and rotting speed of the Manihot esculenta Crantz tuberous root can be delayed, and in addition, the content of the vitamin C and the starch of the Manihot esculenta Crantz tuberous root is not affected. In addition, the method is convenient, practical, efficient and safe and has a good development potential in a storage, transportation and selling process of the Manihot esculentaCrantz tuberous root.