47 results about "Endometrial cell" patented technology

The present invention relates to markers of endometriosis which are differentially expressed in the endometrial cells of females with endometriosis compared to endometriosis-free females. The invention also relates to methods for determining likelihood of endometriosis in female subjects, to methods for grading endometriosis in females suffering from endometriosis and to methods for treating this disease. The invention is also concerned with polynucleotides, probes, primers and kits useful for reducing into practice the above-mentioned methods which are more rapid, non invasive, much less complicated and much less costly than laparoscopy.

The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic endometrial cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the polypeptides of the invention. The invention also relates to compositions containing the nucleic acid molecules, polypeptides, antibodies, agonists and antagonists of the invention and methods for the use of these compositions. These uses include identifying, diagnosing, monitoring, staging, imaging and treating endometrial cancer and non-cancerous disease states in endometrial, identifying endometrial tissue, monitoring and identifying and / or designing agonists and antagonists of polypeptides of the invention. The uses also include gene therapy, production of transgenic animals and cells, and production of engineered endometrial tissue for treatment and research.

The present invention provides biomarkers for the diagnosis and prognosis of endometriosis. Generally, the methods of this invention find use in diagnosing or for providing a prognosis for endometriosis by detecting the expression levels of biomarkers, which are differentially expressed (up- or down-regulated) in endometrial cells from a patient with endometriosis. Similarly, these markers can be used to diagnose reduced fertility in a patient with endometriosis or to provide a prognosis for a fertility trial in a patient suffering from endometriosis. The present invention also provides methods of identifying a compound for treating or preventing endometriosis. Finally, the present invention provides kits for the diagnosis or prognosis of endometriosis.

The present invention belongs to the tissue engineering field and is especially a preparation process of artificial endometrium. New type biodegradable material is used in making stereo rack, endometrium cell of experiment animal is used as seed cell, and artificial endometrium is in vitro constituted via special culture process. The artificial endometrium is a 3D structure, has the similar shapeand function to the in vivo endometrium, can secrete extracellular matrix component and can emulate the configuration and function of in vivo endometrium. The present invention provides the research of embryo nidatin mechanism with ideal platform and provides treating way for endometrium damage caused by induced abortion, tuberculosis, etc.

Provided are a cervical brush and a manufacturing process thereof. The cervical brush comprises a brush handle, a fixing piece and a brush head, the brush head is provided with a connecting rod and bristles, the connecting rod is provided with a blocking position and an end, and the end is provided with a round end portion and a collecting portion. The manufacturing process of the cervical brush comprises the following steps: screening the brush head, manufacturing the end, drying and shaping the end, processing the connecting rod, heating the connecting rod and mounting the brush handle, so that the brush head produced by a method for winding an iron wire is effectively and stably fixed on the brush handle; after successful installation, through quality detection, overall sterilization and cervical brush packaging, the quality and sanitation degree of the cervical brush are guaranteed. According to the manufacturing process of the cervix brush, the brush head and the brush handle can be firmly and fixedly connected, the stability degree of insertion fit connection is improved, the round end portion on the brush head effectively provides a judgment basis of the insertion depth for a user, and the collecting portion can effectively collect cells on the inner wall of the bottom of the uterus, and help is provided for a patient to deeply obtain the endometrial cells.

The invention discloses an endometrial cell sampler which comprises an outer sleeve and an inner rod arranged in the outer sleeve and capable of extending, contracting and moving in the outer sleeve.Each of the head end of the inner rod and that of the outer sleeve is provided with a sampling portion. The endometrial cell sampler has little irritation to an examined muscle body portion and has good sampling effect, and samples on the sampling portions are protected effectively.

A method for growing polarized endometrial cells, said method comprising: (a) disposing endometrial cells on a scaffold, said scaffold comprising a silica-based glass composition, characterized by multi-modal porosity, said scaffold being to define a top side and a bottom side; (b) providing nutrients to said top and bottom sides of said scaffold and an environment to grow polarized endometrial cells on said scaffold.

The invention relates to an adipose stem cell membrane, in particular to a method and application for constructing an adipose stem cell membrane. It belongs to the field of tissue engineering and regenerative medicine. The method comprises the following steps: step 1) selecting adipose stem cells as seed cells to construct cell membranes; step 2) using endometrial cells for induced co-culture cells; step 3) constructing adipose-derived stem cell membranes by means of chemical induction and co-cultivation piece. The present invention utilizes cell patch medium to add exogenous factors (TGF-β1, EGF, PDGF-BB, 17-β estradiol) to co-culture endometrial cells to induce human adipose stem cells to form a film. This patch culture system , the mRNA expression of each epithelial marker in the induced membrane was up-regulated, and immunofluorescence showed that the protein CK18 was positive, suggesting that some stem cells transformed into epithelial cells. The membrane sheets induced by this system are more effective in repairing the endometrial epithelium.

Methods for the detection and diagnosis of endometriosis are disclosed herein. More specifically, sensitive single-cell expression analysis methods to detect specific gene expression patterns in cellpopulations obtained from endometrial cells are provided.

The invention discloses a method for identifying peripheral blood circulation endometrial cells and application. The identification method according to the present invention comprises a step of selecting, as candidate cells, PECAM1-, DAPI +, and PTPRC-from an enriched liquid phase obtained by removing erythrocytes and leukocytes from peripheral blood from a subject, cells in which endometrial markers are positive. According to the method, the identification accuracy of the circulating endometrial cells can be greatly improved.