11 results about "Endometrial cell" patented technology

Disclosed are methods for producing controlled endometrial organoids using micropatterning techniques, and methods for using endometrial cells or organoids for diagnostic and predictive purposes, and for optimizing IVF and other infertility-related treatments, including embryo transfer and intrauterine insemination (IUI). These organoids grow in a spatially controlled manner and exhibit less heterogeneity than organoids grown directly in a three-dimensional culture. The specification also describes methods for predicting the success of embryo transfer or other IVF-related treatments, optimizing embryo transfer, improving embryo implantation in the endometrium, and performing embryo transfer in subjects. These methods include culturing endometrial organoids or endometrial epithelial cells, and contacting the endometrial organoids or cells with one or more blast-like cells. In some embodiments, the endometrial organoids or cells include endometrial cells obtained from subjects undergoing or considering IVF-related treatment.

The invention discloses a system for endometriosis diagnosis and a computer storage medium. Aiming at major adjustment of endometriosis diagnosis standards, biomarkers and ploidy information of circulating endometrial cells in easily available samples such as peripheral blood of a patient are detected, and comprehensive risk assessment is performed by utilizing an optimization algorithm model, so that the diagnosis accuracy of endometriosis is improved. The invention provides an objective and quantifiable noninvasive diagnostic tool adapted to the adjusted standard, and the method solves the problem of missed diagnosis caused by false negative in the existing surgical diagnostic method, and has important clinical application value, social benefit and market prospect for improving the overall diagnostic level of endometriosis.

The application discloses a hydrogel for endometrial repair, which is prepared from platelet-rich plasma, benzene boronic acid modified hyaluronic acid methacrylate (HAMA-PBA), phenyl-2,4,6-trimethyl benzoyl phosphonic acid lithium (LAP), calcium chloride (CaCl2) and polyvinyl alcohol (PVA). The application further provides a preparation method of the hydrogel, which comprises the following steps: mixing HAMA-PBA aqueous solution, LAP aqueous solution and CaCl2 aqueous solution to obtain a precursor solution A, dissolving PVA in PRP to obtain a PVA-PRP mixed solution as a precursor solution B, mixing the precursor solution A and the precursor solution B at a volume ratio of 1:1 to obtain PRP@HAMA-PBA-PVA hydrogel, and irradiating the PRP@HAMA-PBA-PVA hydrogel under ultraviolet light to obtain the hydrogel for endometrial repair. The hydrogel can significantly promote endometrial cell proliferation, has strong pro-angiogenic characteristics, inhibits endometrial fibrosis, and thus comprehensively repairs the endometrial microenvironment, promotes endometrial regeneration and improves embryo implantation rate and live birth rate.

The application discloses high-adhesion modified mesenchymal stem cells and application thereof in preparation of a medicine for treating intrauterine adhesion. The modified mesenchymal stem cells are genetically engineered to overexpress integrin alpha 2 subunit and / or integrin alpha 5 subunit, thereby enhancing the adhesion capacity to the extracellular matrix (collagen and fibronectin) of the endometrium. In vitro experiments show that the initial adhesion rate, liquid flushing resistance and anoikis resistance of the modified MSCs are significantly better than those of common MSCs. When used in combination with the "dynamic culture and drug delivery device for intrauterine adhesion treatment" applied by the inventor on the same day, the retention rate of the modified MSCs in the simulated intrauterine dynamic culture environment is significantly improved. Animal experiments show that the modified MSCs in combination with the device can significantly promote endometrial regeneration, inhibit fibrosis and improve the pregnancy rate. The application solves the core bottleneck of low cell retention rate and short survival time in the MSC transplantation treatment of intrauterine adhesion by enhancing the adhesion capacity of the MSCs, and forms a complete treatment scheme in cooperation with the device, thereby having a good clinical application prospect.

The invention relates to the field of high-performance composite materials, in particular to an embryo cryopreservation cell carrier scaffold material and a preparation method of the embryo cryopreservation cell carrier scaffold material. The total amount is 100%: 50.0 to 65.0 weight percent of gelatin, 20.0 to 35.0 weight percent of silk fibroin, 5.0 to 12.0 weight percent of sodium hyaluronate, 3.0 to 8.0 weight percent of chitosan, 0.1 to 2.0 weight percent of RGD polypeptide and 0.5 to 3.0 weight percent of anti-ice crystal forming polypeptide AFP III, the gelatin, hyaluronic acid and silk fibroin ternary composite system simulates biochemical components and physical characteristics of an endometrial extracellular matrix, provides similar in-vivo microenvironment support for embryos, and is beneficial to maintaining embryo activity.

The invention discloses a sampler for collecting endometrial cells or tissue samples and a use method of the sampler, and belongs to the technical field of medical instruments, and the sampler comprises a flexible catheter and a deformable inner core guide wire; the inner core guide wire is made of hyperelastic shape memory alloy, the inner core guide wire is composed of a near-end rod body and a far-end self-adaptive brush head, and when the far end of the inner core guide wire is pushed out of the flexible catheter, the self-adaptive brush head is automatically unfolded to form an inverted triangular frame; a plurality of flexible bristles with Shore A hardness of 10A-30A are distributed on the self-adaptive brush head in a three-dimensional gradient manner; the invention further provides an application method. According to the invention, through the combination of the'automatically unfolded inverted triangular brush head 'and the'three-dimensional gradient distributed soft bristles', the shape self-adaption and full-coverage sampling of the uterine fundus and the uterine horn of the uterine cavity are realized, and the omission ratio is obviously reduced; the ultra-thin flexible catheter is combined with the extremely-soft bristles, so that the full-path noninvasive and safe operation from the hymen to the endometrium is ensured, and the applicable crowd is expanded.

The invention provides a pharmaceutical preparation for repairing endometrial injury, and belongs to the technical field of biomedicine. The pharmaceutical preparation takes improved polypeptide as an active ingredient, and the amino acid sequence of the improved polypeptide is shown as SEQ ID NO.5. Experimental results show that the polypeptide can significantly promote proliferation and migration of human endometrial stromal cells, still maintains low cytotoxicity under high concentration, and shows excellent safety. Furthermore, in a rat endometrial mechanical injury model, the preparation can obviously increase the endometrial thickness, improve the Ki-67 positive cell rate, enhance the proliferation activity of endometrial cells and promote the repair of damaged tissues. Compared with the original polypeptide, the preparation disclosed by the invention shows unexpected technical effects in the aspects of repairing activity and safety, and has a good application prospect.

The utility model relates to a collector applied to endometrial cells and tissues. The collector comprises a catheter, an elastic skeleton, a movable strip, a seat body and a flow channel structure, a medical worker guides the insertion end of the catheter into the uterine cavity of a patient, then the elastic framework at the connecting end is gradually pushed out of the insertion end by pushing the operation end of the movable strip, the elastic framework is outwards unfolded under the action of self elasticity, and the medical worker can drive the movable strip and the elastic framework to synchronously rotate by rotating the operation end; and the cells and tissues of the endometrium can be scraped by the outline of the elastic skeleton, so that the cells and tissues fall into the uterine cavity. After the operation of scraping the cells and the tissues of the endometrium is completed, a medical worker can connect an upper negative pressure device at the collection interface on the seat body, and the scraped cells and the tissues in the uterine cavity are fully extracted through the flow channel structure, so that the collection of the cells and the tissues of the endometrium is completed. Compared with the prior art, the collector has the advantage of high collection efficiency.

This invention relates to an endometrial cell collector, comprising an outer sheath, a sampling handle, and sampling brushes. The sampling handle is inserted inside the outer sheath, with one end extending out of the sheath. Multiple sampling brushes are arranged side-by-side, each rotatably mounted within the sampling handle and radially arranged along it. The length of each sampling brush is greater than the length of the sampling handle, with both ends extending out of the handle. Each sampling brush has a brush head at one end. When the sampling handle approaches the outer sheath axially, the brush head extends out of the sheath, and the extended brush heads are arranged in a fan shape to expand the contact area with the uterine cavity, reaching both uterine horns. The multiple sampling brushes arranged side-by-side within the outer sheath can extend and retract under the push and pull of the sampling handle. The fan-shaped arrangement of the extended brush heads ensures coverage throughout the entire uterine cavity, preventing missed sampling.

The application discloses an endometrium-targeted mitochondrial function compensation system and application thereof. The endometrium-targeted mitochondrial function compensation system comprises a function unit, a delivery unit and a control unit. The function unit comprises a vesicle preparation formed by wrapping donor mitochondria with red blood cell membranes. The donor mitochondria are isolated mitochondria with complete inner and outer mitochondrial membranes and ridge structures or engineered mitochondria carrying exogenous mitochondrial DNA. The application first constructs a comprehensive system integrating biological active ingredients, targeted delivery and intelligent control, can directly and efficiently repair the mitochondrial function of endometrial cells, promote endometrial proliferation, and provides an innovative solution for treating related reproductive diseases. The system has complete design, clear effect and good clinical transformation prospect.

The invention relates to an establishment and verification method of an infertility endometrial organoid. The method comprises the steps of endometrial cell extraction, organoid construction, passage and identification. The method comprises the following steps: digesting and dissociating biopsy tissues of a patient to obtain single cells, re-suspending the single cells in matrigel, culturing the single cells in an ExM culture medium for 7 days to form three-dimensional organs, and after passage, detecting specific markers through immunofluorescent staining, detecting immune cell factors through Luminex and analyzing gene expression through qPCR to verify functional differences. The method has the advantages that the organoid constructed by the method can really simulate the endometrial structure function and immune microenvironment, the hereditary heterogeneity of a patient is reserved, the culture period is short, and the cost is low; the method can be clinically used for etiology analysis, embryo implantation potential prediction and medication scheme optimization of IVF-ET repeated failure patients, and an efficient platform is provided for accurate diagnosis and treatment of infertility, drug screening and endometrial injury repair research.