Cell handling device, human tissue regeneration composition,and human tissue regeneration method

A treatment device and cell technology, which is applied in the field of regenerative medicine, can solve problems such as difficulty in cell transportation, difficult implementation of cell transplantation therapy, troublesome differentiation induction process, etc., and achieve the effects of saving cumbersomeness, good regenerative medicine, and controlling the speed of internal pressure change

Inactive Publication Date: 2006-11-08
JMS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, in the cell transplantation of regenerative medicine, not only a series of processes such as cell collection, culture, differentiation induction, and transplantation into the living body are cumbersome, but also in order to prevent contamination (contamination) from the surroundings, it must be done in a clean environment, And use skilled cutting-edge technology to carry out a series of operations
Therefore, it is difficult to perform cell transplantation therapy without well-equipped facilities and personnel with cutting-edge skills
In addition, in addition to the operation, it is also difficult to transport the collected or cultured cells to specified equipment such as sterile rooms or incubators without being polluted by the surrounding environment.

Method used

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  • Cell handling device, human tissue regeneration composition,and human tissue regeneration method
  • Cell handling device, human tissue regeneration composition,and human tissue regeneration method
  • Cell handling device, human tissue regeneration composition,and human tissue regeneration method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0077] 1-1. Structure of the syringe-type cell processing device 1

[0078] figure 1 Shown is a configuration diagram of a syringe-type cell processing device 1 as an example of Embodiment 1 of the cell processing device of the present invention. figure 1 (A) is a perspective view, figure 1 (B) is a side view, figure 1 (C) for figure 1 (B) X-X' section view.

[0079] figure 1 The structure of the illustrated syringe-type cell processing device 1 is roughly divided into a syringe body 2 , a plunger (also referred to as a plunger or piston) 40 , and the like. A cell suspension 100 is stored in the syringe main body 2 .

[0080] The syringe main body 2 is composed of a cylindrical body 3 formed by injection molding a cell non-adhesive material into a cylindrical shape, and a gas permeable membrane 20 described later.

[0081] The front end side of the cylindrical body 3 is formed with a disc-shaped front end surface 110 and a bait portion 120 protruding thereon. The front...

Embodiment approach 2

[0118] figure 2 A cross-sectional view showing the structure of a syringe-type cell processing device 1 according to Embodiment 2 as an example of the cell processing device of the present invention is shown. Unlike Embodiment 1, in the syringe main body 2 , no through-hole is provided in the peripheral wall of the cylindrical body 3 , but an air-permeable region is formed on the front end surface 1100 of the cylindrical body 3 . This air-permeable region, specifically, when forming the syringe main body 2, can be that only the front end surface 1100 is made of an air-permeable material, and can also be formed by the following method: opening a through hole on the front end surface 1100, fusing and adhering Alternatively, a gas-permeable film is bonded to cover the through hole, thereby providing liquid-tightness.

[0119] The same materials as those listed in Embodiment 1 can be used for the above-mentioned cell non-adhesive material and the above-mentioned air-permeable me...

Embodiment approach 3

[0126] image 3 A cross-sectional view showing the structure of a syringe-type cell processing device 1 according to Embodiment 3 of the cell processing device of the present invention. The difference from the first and second embodiments described above is that the syringe body 2 is made of the same cylindrical body as the conventional syringe body, and the piston head 44 is made of a gas-permeable material. As the air-permeable material, those listed in Embodiment 1 or 2 above can be used.

[0127] The piston head 44 is fitted and fixed to the syringe-side front end of the main body 42 of the piston 40 so as to be fluid-tightly connected to the inner wall of the syringe main body 2 . Here, the piston head 44 is only selectively permeable to gas, so the solution of the cell suspension 100 will not leak to the outside.

[0128] Also in the third embodiment, it is preferable to use the above-mentioned cell non-adhesive material to form the syringe main body 2 and the plunger ...

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Abstract

A cell handling device capable of storing and carrying sampled or cultured cells without being contaminated from the surroundings in regeneration treatment and easily injecting the cells in the living body, wherein the cells are sampled from the living body, the sampled cells or the cells provided by culturing the cells are stored in a syringe type storage container, and the cells stored in the storage container are transplanted into the living body. The cell storage container is desirably such that a part or more of the inner wall of the container in contact with the cells is formed with a cell unbonded material. Since the cells can take in oxygen required for the living thereof in the container and are prevented from being adhered to the inner wall of the container, the cells can be easily and rapidly transplanted to the living body without applying cell releasing treatment to the cells when the cells are used in the field of the regeneration treatment.

Description

technical field [0001] The present invention relates to regenerative medicine, in particular to a cell treatment device and a composition for tissue regeneration used in regenerative medicine. Background technique [0002] In recent years, with the advancement of molecular cell biology and cell engineering, differentiated cells or cells (stem cells) with multi-differentiation function and self-replication ability taken from the living body are transplanted into patients for treatment. research has also been ongoing. The so-called regenerative medicine refers to a treatment method in which differentiated cells or stem cells are transplanted into the defective part of the biological tissue or the responsible disease site of the disease to repair and restore the target tissue (including organs). In general, in regenerative medicine, collected cells are temporarily cultured alone or in a dedicated container in the presence of a scaffold, and differentiated or undifferentiated c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/36G01N33/48
CPCC12M45/22C12M23/24
Inventor 弓削类松浦洋治栈敷俊信
Owner JMS CO LTD
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