Apparatus and method for perfusion culture

Abstract

A bioreactor vessel incudes a first compartment configured to receive a suspension comprising cells and a cell culture medium, for use in a cell processing operation, a second compartment for receiving an overflow of the cell culture medium from the first compartment, and an overflow separating the first compartment from the second compartment, the overflow being configured to maintain a level of the cell culture medium in the first compartment.

Description

technical field

[0001] Embodiments of the invention relate generally to bioprocessing apparatus, systems, and methods, and more particularly to a bioreactor vessel and a method of perfusion for a bioreactor vessel. Background technique

[0002] Various vessels, devices, components and unit operations are known for performing biochemical and / or biological processes and / or manipulating liquids and other products of such processes. Such biological processes can be used, for example, in the manufacture of cellular immunotherapies such as chimeric antigen receptor (CAR) T cell therapy, which redirects a patient's T cells to specifically target and destroy tumor cells. As is known in the art, the manufacture of cellular immunotherapy, such as CAR T cell therapy, may involve the extraction, activation, genetic modification, culture and expansion of cells in one or more bioreactor vessels.

[0003] Recent advances in the manufacture of cellular immunotherapies have provided for the...

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