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Proliferation culture medium for 3D simulation culture of stem cells

A proliferation medium and stem cell proliferation technology, applied in the field of proliferation medium for 3D simulation culture, to achieve the effect of promoting efficiency and survival rate

Pending Publication Date: 2022-02-18
华子昂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above problems, the present invention provides a proliferation medium for stem cell 3D simulation culture, which has the characteristics of clear composition, good use effect and low price, and is formed based on statistical analysis of a large number of stem cell 3D simulation culture experiment data, which can solve the problem of stem cell The problem of proliferation medium for 3D simulation culture of stem cells that is urgently needed for industrial development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Proliferation medium for 3D simulation culture of stem cells used, with an osmotic pressure of 300 mOsmol / kg H 2 O, pH7.4, its composition includes: inorganic salts, amino acids, sugars, lipids, nucleosides, vitamins, cell metabolites, cell proliferation factors, water, the specific ingredients are listed as follows by type:

[0025] Inorganic salts: potassium ion 0.03%, sodium ion 0.5%, calcium ion 0.01%, magnesium ion 0.003%, iron ion 0.0001%, bicarbonate ion 0.16%, sulfate ion 0.001%, phosphate ion 0.02%, chloride ion 0.4%.

[0026] Natural amino acids: alanine 0.0005%, arginine 0.0005%, asparagine 0.0005%, aspartic acid 0.0005%, cysteine ​​0.0005%, glutamine 0.03%, glutamic acid 0.0005%, glycine 0.0005% , Histidine 0.0005%, Isoleucine 0.0005%, Leucine 0.0005%, Lysine 0.0005%, Methionine 0.0005%, Phenylalanine 0.0005%, Proline 0.0005%, Serine 0.0005%, Threonine 0.0005%, Tryptophan 0.0005%, Tyrosine 0.0005%, Valine 0.0005%.

[0027] Sugars: glucose 0.1%, sodium pyr...

Embodiment 2

[0040] Proliferation medium for 3D simulation culture of stem cells used, with an osmotic pressure of 270 mOsmol / kg H 2 O, pH7.2, its composition includes: inorganic salts, amino acids, sugars, lipids, nucleosides, vitamins, cell metabolites, cell proliferation factors, water, the specific ingredients are listed as follows by type:

[0041] Inorganic salts: potassium ion 0.01%, sodium ion 0.6%, calcium ion 0.003%, magnesium ion 0.0002%, iron ion 0.0002%, bicarbonate ion 0.1%, sulfate ion 0.0005%-0.005%, phosphate ion 0.005% -0.05%, Chloride 0.5%.

[0042] Natural amino acids: alanine 0.01%, arginine 0.03%, asparagine 0.01%, aspartic acid 0.01%, cysteine ​​0.01%, glutamine 0.06%, glutamic acid 0.01%, glycine 0.01% , Histidine 0.01%, Isoleucine 0.01%, Leucine 0.01%, Lysine 0.01%, Methionine 0.01%, Phenylalanine 0.01%, Proline 0.01%, Serine 0.01%, Threonine 0.01%, Tryptophan 0.01%, Tyrosine 0.01%, Valine 0.01%.

[0043] Sugars: glucose 0.01%, sodium pyruvate 0.05%.

[0044] L...

Embodiment 3

[0056] Proliferation medium for 3D simulation culture of stem cells used, with an osmotic pressure of 330 mOsmol / kg H 2 O, pH7.4, its composition includes: inorganic salts, amino acids, sugars, lipids, nucleosides, vitamins, cell metabolites, cell proliferation factors, water, the specific ingredients are listed as follows by type:

[0057] Inorganic salts: potassium ion 0.02%, sodium ion 0.7%, calcium ion 0.004%, magnesium ion 0.01%, iron ion 0.0002%, carbonate ion 0.1%, sulfate ion 0.005%, phosphate ion 0.05%, chloride ion 0.6 %.

[0058] Natural amino acids: alanine 0.01%, arginine 0.03%, asparagine 0.01%, aspartic acid 0.01%, cysteine ​​0.01%, glutamine 0.06%, glutamic acid 0.01%, glycine 0.01% , Histidine 0.01%, Isoleucine 0.01%, Leucine 0.01%, Lysine 0.01%, Methionine 0.01%, Phenylalanine 0.01%, Proline 0.01%, Serine 0.01%, Threonine 0.01%, Tryptophan 0.01%, Tyrosine 0.01%, Valine 0.01%.

[0059] Sugars: glucose 0.01%, sodium pyruvate 0.05%.

[0060] Lipids: Lecithin...

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Abstract

The invention relates to a proliferation culture medium for 3D simulation culture of stem cells, the proliferation culture medium is free of serum addition, clear in composition and suitable for 3D simulation perfusion culture of stem cells, and comprises inorganic salt, amino acid, saccharides, lipids, nucleosides, vitamins, cell metabolites, cell proliferation factors and water, the osmotic pressure of a culture solution is 270-330mOsmol / kg.H2O, and the pH of the culture solution is 7.2-7.4.

Description

technical field [0001] The invention belongs to the field of medicine, in particular to a proliferation medium for 3D simulation culture. Background technique [0002] Embryonic stem cells are a kind of primitive undifferentiated cells with unlimited proliferation ability and unlimited differentiation potential. They are the core driving force of contemporary medical progress and medical efficiency improvement. Mesenchymal stem cells and adult stem cells are currently the most popular stem cell research directions. They have the advantages of easy acquisition, easy scale-up and culture, strong differentiation ability, and immune regulation. Therefore, they have made great progress in regenerative medicine and stem cell therapy and have shown great potential. market application potential. [0003] The 2D culture technology based on petri dishes and culture bottles has made great contributions to stem cell culture, completed the technical transition of stem cells from human b...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
CPCC12N5/0662C12N2500/12C12N2500/14C12N2500/16C12N2500/24C12N2500/32C12N2500/34C12N2500/30C12N2500/36C12N2500/40C12N2500/38C12N2501/11C12N2501/135C12N2513/00C12N2500/35C12N2500/90
Inventor 华子昂刘宝全朱美瑛万君兴竹添
Owner 华子昂
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