Filtering-precipitation dual perfusion system for animal cell perfusion culture

A technology of perfusion culture and animal cells, which is applied in the field of cell engineering and biopharmaceuticals, can solve the problems of high cost, short use time, limited retention efficiency, etc., achieve low cost, simple structure, reduce the probability of filtration load and clogging Effect

Active Publication Date: 2007-12-12
石药集团明复乐药业(广州)有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The interception devices that have been reported so far include built-in or external filters, settling devices, hollow fibers, ultrasonic filters, centrifuges, etc. Some of these interception devices have limited interception efficiency, some have high cost, and some have a short service life. long, there are still some deficiencies that cannot meet the needs of all parties

Method used

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  • Filtering-precipitation dual perfusion system for animal cell perfusion culture
  • Filtering-precipitation dual perfusion system for animal cell perfusion culture

Examples

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Embodiment Construction

[0019] As shown in Figures 1 and 2, a filtration-sedimentation dual-combination perfusion system for animal cell perfusion culture provided by the present invention includes a filtration-sedimentation two-in-one perfusion column, peristaltic pumps 5 and 6, an automatic controller 9, Three-way pipeline 10 and pipeline clamp 12, the described filtration-sedimentation two-in-one perfusion column is connected with peristaltic pump 5,6 through three-way pipeline 10, and peristaltic pump 5,6 is operated by automatic controller 9 control, and pipeline clamp 12 is set On the pipeline connected between the filtration-sedimentation two-in-one perfusion column and the peristaltic pump. The filtration-sedimentation two-in-one perfusion column includes an inner column 1 and a jacket 2, the inner column 1 is set inside the jacket 2, and the inner column 1 is composed of a filter screen 11 with a pore size of 20-70 μm, a bracket 14 and a liquid outlet tube 13, and the filter screen The mater...

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Abstract

The invention discloses one filteration- deposition combined perfusion system used for animal cell perfusion culture. It comprises filtering- deposition- perfusion column, peristaltic pump, automatic controller, tri- way pipeline and pipe clamp; filtering- deposition- perfusion column is connected with peristaltic pump through tri- way pipeline, the rotation of peristaltic pump is controlled by automatic controller, pipe clamp is installed on pipe between filtering- deposition- perfusion column and peristaltic pump. The invention is characterized by simple structure, low cost, high collecting efficiency, long life length and wide application on micro- carrier or cluster animal cell perfusion culture.

Description

technical field [0001] The invention relates to a filtration-sedimentation double combined perfusion system for perfusion culture of animal cells, belonging to the fields of cell engineering and biopharmaceuticals. Background technique [0002] There are more and more varieties of genetically engineered drugs produced by animal cells, especially the emergence of engineered antibodies, and the output has become the "bottleneck" of production. There has been increasing interest in replacing traditional batch or batch-fed production processes with perfusion culture systems. The use of a perfusion culture system can greatly increase cell density and yield, and greatly reduce the investment in equipment and delivery rooms. In order to carry out effective perfusion culture, its core is to have a high-efficiency, low-cost, and long-term use perfusion system that traps cells in the reaction tank. The interception devices that have been reported so far include built-in or external ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/12
Inventor 肖成祖杨琴李智陆进丽梁倩茹陈小飞
Owner 石药集团明复乐药业(广州)有限公司
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