High-throughput detection method for metal ions and metal nanoparticles based on single cell enrichment and single cell mass spectrometry

Abstract

The invention discloses a high-throughput detection method for metal ions and metal nanoparticles based on single cell enrichment and single cell mass spectrometry. The invention provides a method fordetecting the metal ions and metal nanoparticles. The method comprises the following steps: absorbing and enriching the metal ions and the metal nanoparticles by utilizing cells or unicellular organisms; marking the treated cells or single-cell organisms by using a metal isotope A, wherein the metal isotope A is other metal isotopes except a to-be-detected metal element and can be used for marking survival and dead cells or single-cell organisms at the same time; and then carrying out single-cell mass spectrometry flow detection to obtain an intracellular signal intensity value of the to-be-detected metal element, and further calculating to obtain the content of the to-be-detected metal element in a to-be-detected sample. The sample pretreatment is simple, and no harmful reagent is introduced; the demand on samples is very small; the flux is high; the sensitivity is high; cell-related metal signals may be differentiated.

Description

technical field [0001] The invention relates to the field of detection of metal ions and metal nanoparticles, in particular to a high-throughput detection method of metal ions and metal nanoparticles based on single-cell enrichment and single-cell mass spectrometry. Background technique [0002] Atomic absorption spectrometry (AAS), inductively coupled plasma-atomic emission spectrometry (ICP-AES) and inductively coupled plasma mass spectrometry (ICP-MS) are currently commonly used elemental analysis methods. For the detection of metal nanoparticles, the commonly used methods are laser ablation-inductively coupled plasma mass spectrometry (LA-ICP-MS), single particle / single cell-inductively coupled plasma mass spectrometry (SP / SC-ICP-MS) Wait. The AAS analysis method needs to change the lamp source for the determination of different elements, and only one element can be determined at a time, and multiple elements cannot be analyzed at the same time, and the efficiency of sa...

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Problems solved by technology

[0004] In the process of realizing the present invention, the applicant found that the design of the above-mentioned patent 201910246159.4 has the following limitations: (1) the cell sample needs to be made into a single-cell array before it can be detected, and the sample pretreatment is more complicated; (2) although the combined isotope dilution method, but due to the use of LA-ICP-MS as a detector, its detection throughput is low; (3) for single-cell samples, its detection limit is high, at the fg / cell level, it is difficult to achieve some trace or super Detection of Trace Samples

In the process of realizing the present invention, the applicant found that the design of the above-mentioned patent 201910246159.4 has the following limitations: (1) The sample consumption is large, and it needs to consume tens or even hundreds of milliliters of samples in the process of realizing metal adsorption. Not applicable; (2) The required biomass is large, and each sample needs to consume 100mL algae solution; (3) The detection limit of this method is high, and the uniformity of the sample is high, otherwise the repeatability of the sample detection is poor

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