Joint application of three types of monoclonal antibody-coupled immunomagnetic beads to sorting tumor cells

Abstract

The invention discloses joint application of three types of monoclonal antibody-coupled immunomagnetic beads to sorting tumor cells and a method for sorting breast cancer tumor cells. The three types of monoclonal antibody-coupled immunomagnetic beads include anti-HLA-G (human leukocyte antigen-G) monoclonal antibody-coupled immunomagnetic beads, anti-EpCAM (epithelial cell adhesion molecule) monoclonal antibody-coupled immunomagnetic beads and anti-CK8 / 18 (casein kinase 8 / 18) monoclonal antibody-coupled immunomagnetic beads. The joint application and the method have the advantages that the three types of jointly applied antibody-coupled immunomagnetic beads are high in breast cancer tumor cell enrichment ratio and good in sensitivity and repeatability, and the relevant tumor cells can be detected; the three types of immunomagnetic beads (the anti-EpCAM immunomagnetic beads, the anti-HLA-G immunomagnetic beads and the anti-CK8 / 18 immunomagnetic beads) are jointly applied to enriching the tumor cells, so that the circulating tumor cell coverage rate can be increased to the greatest extent, the enrichment ratio can reach 80%, and a technology is international pioneer; the tumor cell enrichment specificity and sensitivity can be particularly guaranteed owing to usage of the anti-HLA-G monoclonal antibody-coupled immunomagnetic beads.

Description

technical field [0001] The present invention relates to a method for sorting tumor cells, in particular to a kind of anti-HLA-G antibody as the core, three anti-HLA-G, EpCAM, CK8 / 18 antibody-coupled magnetic beads, combined with three Method for sorting breast tumor cells by immunomagnetic beads coupled to antibodies. Background technique [0002] The number of circulating tumor cells in peripheral blood is very small, usually only a few tumor cells in about 100 million white blood cells and 50 billion red blood cells, and there is only one circulating tumor cell in about every 105-107 monocytes. Therefore, in order to improve the detection rate of circulating tumor cells, it is usually necessary to enrich circulating tumor cells before detection. Cell enrichment can be achieved by specific markers of tumor cells (immunoisolation) or by cell morphology characteristics such as cell volume and density. [0003] Commonly used cell enrichment techniques mainly include immuno...

AI Technical Summary

Problems solved by technology

The disadvantage of these techniques is that they use too little blood and must be diluted with the corresponding isotonic working fluid. If the experiment is carried out according to the blood volume commonly used in clinical practice, the working fluid volume can reach 70ml, and the passing speed is only 0.5. ml / h, the dilution process of the liquid will also cause the loss of circulating tumor cells

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