Synchronous quantum dot fluorescence immunological detection method and kit of multiple small molecular compounds

Abstract

The invention provides an indirect competitive quantum dot fluorescence immunological detection method for synchronously detecting multiple small molecular compounds and detection kit, wherein the immunological detection method is a liquid phase immunological detection method which uses an encoded microsphere as a solid phase carrier and a quantum dot as a fluorescent marker and is used for competitive specificity reactions of a small molecular compound antigen. c Firstly, a captured antigen is covalently bound to the surface of the encoded microsphere, an indirect competitive fluorescent immune complex of microsphere-captured antigen-detected antibody-second antibody-quantum dot is formed in a filter film plate reaction hole through capturing the antigen, detecting the antibody and binding the second antibody specificity, and then the indirect competitive fluorescent immune complex flows across a suspension chip or a detection region of a flow analysis system one by one under the restraint of the sheath fluid, recognizes different encoded microspheres and detects the fluorescent intensity (or average fluorescence intensity) of the quantum dot to complete the detection. A plurality of small molecule compounds in the same sample can be synchronously detected, and one or more small molecule compounds in different samples can also be detected. The invention has the advantages of fast speed and high flux.

Description

technical field [0001] The invention relates to a microsphere-based small molecule multi-residue fluorescence immunoassay method, in particular to an indirect competitive immunoassay method using quantum dots as fluorescent markers and polystyrene fluorescently encoded microspheres as solid phase carriers and its application kit. Background technique [0002] Food monitoring has attracted more and more attention from countries all over the world and the public, and residue detection technology has always been an important hot spot in the field of international agricultural product quality and safety. Common analysis methods are mostly instrumental analysis methods used in laboratories, such as gas chromatography (GC), high performance liquid chromatography (HPLC), thin layer chromatography (TLC), mass spectrometry (MS), tandem mass spectrometry (MS-MS) and The combination technology between various methods, etc.; the application of immunochemical technology to the detection...

AI Technical Summary

Problems solved by technology

[0007] The present invention uses quantum dots for fluorescent labeling and is applied to a fluorescent immunoassay method for specific competitive reactions of antigens and antibodies. Polystyrene microspheres are coded according to the ratio of fluorescent dyes embedded inside, and microspheres with different codes are selected as the method. Solid phase carrier, connected with different capture antigens, reacted in the reaction wells of the 96-well filter plate, and simultaneously detected single or multiple small molecule compounds in different samples, which can overcome the disadvantage that ELISA can only detect a single target, Designed to increase detection throughput and increase detection speed