Basophilic granulocyte activation and degranulation identification method

Abstract

The invention provides a basophilic granulocyte activation and degranulation identification method. The basophilic granulocyte activation and degranulation identification method comprises the following steps that test samples are numbered in sequence, and flow type sample feeding pipes required by testing are marked; a required anti-human CD123, anti-human CCR3, anti-human HLA-DR, anti-human CD203c and / or anti-human CD63 fluorescence labeling flow type antibody combination is added into each flow type sample feeding pipe; mixed whole blood is added into the marked flow type pipes, and shading incubation is conducted at the indoor temperature; red blood cell lysate is added into each sample pipe, and shading incubation is conducted at the indoor temperature again; supernatant is removed in a centrifugal mode after incubation; detection is conducted by means of a flow cytometry, and the number of basophilic granulocytes and the average fluorescence intensity are analyzed. According to the basophilic granulocyte activation and degranulation identification method, 80%-100% of the basophilic granulocytes in peripheral blood can be distinguished from other types of cells under the condition that the basophilic granulocytes do not need to be separated or purified, the basophilic granulocyte activation and / or degranulation state can be identified, whole blood of no more than 100 microlitres is needed by each sample to be tested, and the repeatability is good.

Description

technical field [0001] The invention relates to the technical field of allergic disease diagnosis, in particular to an identification method for basophil activation and degranulation. Background technique [0002] The incidence of allergic diseases accounts for more than 30% of the world's total population, and is listed by the World Health Organization as one of the four major non-infectious diseases in the 21st century. With the development of industrial economy and the change of ecological environment, such diseases have been increasing in recent years, becoming common and frequently-occurring diseases, which is a major problem that needs to be solved in the field of health and economic development in our country. [0003] Historically, most of the definitions and diagnostic schemes of diseases were proposed by developed countries. In 2011, He Shaoheng and Zhang Huiyun took the lead in revising and discussing the definition of allergic diseases that had been used for nea...

AI Technical Summary

Problems solved by technology

Unfortunately, to date there is no reliable and reproducible method for specifically identifying the activated, degranulated state of basophils in whole blood, leading to allergen-specific basophil priming. The test cannot be carried out

Our recent study found that if CD123, HLA-DR, and CCR3 are detected simultaneously, 98-100% of the CCR3+CD123+HLA-DR- cell population is basophils, which is the first to basically solve the specificity of basophils. sexual immune recognition, but did not address the identification of basophil activation and degranulation

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