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Preparation method of cortex albiziae neolignan monomeric compound

A compound, Albizia Julibrissin technology, applied in the field of biochemistry, can solve the problems of unclear pharmacological and pharmacodynamic activities, difficult compounds, etc.

Active Publication Date: 2019-12-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, compounds such as triterpenoids, flavonoids, and lignans have been isolated from the bark of Albizia Julibrissin. Since the pharmacological and pharmacological activities of each compound are not yet clear, and the molecular weight or functional group structure of similar compounds is similar, it is suitable for targeted separation and extraction. compounds that pose certain difficulties

Method used

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  • Preparation method of cortex albiziae neolignan monomeric compound
  • Preparation method of cortex albiziae neolignan monomeric compound
  • Preparation method of cortex albiziae neolignan monomeric compound

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Embodiment 1

[0047] The preparation of embodiment 1 compound Aj4

[0048](1) Extraction: take 20 kg of dried Albizia Julibrissin skin, pulverize it, use 5 times of 75% ethanol (water), ie 100 L each time, and extract twice at 80° C. for 2 hours each time. The residue of the bark of Albizia Julibrissin was removed by filtration, the 75% ethanol extract of the bark of Albizia Julibis was combined, and freeze-dried to obtain 1.6 kg of crude ethanol extract of the bark of Albizia Julibis. Grind the crude extract and suspend it in 2L deionized water to dissolve it as much as possible. After suspension, extract with ethyl acetate and saturated n-butanol successively. The extraction adopts the principle of adding ethyl acetate and saturated n-butanol several times each time, combining the extracts of ethyl acetate phase and saturated n-butanol phase respectively. Extracts of ethyl acetate and n-butanol fractions were obtained.

[0049] (2) Separation: take 254g of n-butanol, dissolve the suspen...

Embodiment 2

[0063] The preparation of embodiment 2 compound Aj5

[0064] The step of extraction and separation is with reference to embodiment 1, according to Figure 6 The retention time of each component in the analytical high performance liquid chromatogram, determine the CH 2 Cl 2 :CH 3 The elution condition of the OH=8:1 elution section through the reverse silica gel column (Davisil C18, 50μm) is t R = CH corresponding to 22min, 24min, 26min, 60min 3 OH concentration is 39.8%, 42.9%, 46.2%, 100%, because the diameter of the C18 filler used is 50 μm, so CH 3 The concentration of OH needs to be subtracted by 10%, that is, 29.8%, 32.9%, 36.2%, and 100%. Elution was carried out with methanol and water as the elution phase. according to Figure 6 Determine the elution phase combination as 29% CH 3 OH(H 2 O), 32% CH 3 OH(H 2 O), 36% CH 3 OH(H 2 O), 100% CH 3 OH. Analytical HPLC detection was carried out for each eluted component, and the separation conditions were explored to...

Embodiment 3

[0073] The preparation of embodiment 3 compound Aj6

[0074] Extraction, separation condition are the same as embodiment 2, semi-preparative separation chromatogram is as Figure 11 shown. to 32% CH 3 The OH elution section is subjected to semi-preparative liquid phase separation (the chromatographic column is X-Bridge C18, 5μm, 10×250mm, the flow rate is 4ml / min, and the column temperature is 30°C), such as Figure 15 Shown (UV detection wavelength 290nm), collection retention time t R = The compound at 35.20 min was rotated under reduced pressure at 75°C, the solvent was recovered, and the monomer compound Aj6 was obtained by drying in a vacuum oven.

[0075] Structural identification: Aj6 is a light yellow powder, detected by UPLC-ESI-MS, and its mass spectrum is as follows Figure 12 shown. ESI-MS m / z 617[M+Cl - ], through Monoisotopic Mass, Even Electron Ions determine its molecular formula is C 28 h 38 o 13 .

[0076] Dissolve the sample Aj5 in a nuclear magnet...

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Abstract

The invention discloses a preparation method of a cortex albiziae neolignan monomeric compound and belongs to the technical field of biochemistry. The prepared compound can effectively treat FFAs-induced lipid metabolism disorder and fatty degeneration and can reduce the lipid droplet area by about 3 times at the administration concentration of 20 [mu] M 24 h after administration; the compound cansignificantly enhance cell viability of HUVEC and significantly promote self replication of cells so as to promote proliferation of endothelial cell HUVEC. The cell viability can reach 242.233% in control at the administration concentration of 40 [mu] M 24 h after administration. The prepared compound can remarkably inhibit HG-induced reactive oxygen species (ROS) of the HUVEC cells and reduce the average flurescence intensity of an HG group to 176.36% from 818.37%.

Description

technical field [0001] The invention relates to a preparation method of a new lignan compound of Albizia juliensis, belonging to the technical field of biochemistry. Background technique [0002] Albiziae Cortex is the bark of Albzia julibrissin Durazz, a leguminous plant. It is a commonly used traditional Chinese medicine. In recent years, with the in-depth research of Albizia Julibrissin by scholars, its chemical composition and pharmacological and pharmacological activities have been continuously discovered. [0003] At present, the research on the active compounds in Albizia Julibrissin is mainly extracted and separated by chemical methods, such as extraction with organic solvents such as ethanol, and elution with macroporous resins, silica gel column chromatography, and reverse C18 chromatographic separation. So far, compounds such as triterpenoids, flavonoids, and lignans have been isolated from the bark of Albizia Julibrissin. Since the pharmacological and pharmacolo...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H15/203C07H1/08
CPCC07H1/08C07H15/18C07H15/203
Inventor 邱丽颖史学林艾敏蔡维维李忠杰刘艺筱
Owner JIANGNAN UNIV
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