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Inductive agent for inducing marine bivalve mollusk to produce eggs as well as application method thereof

A bivalve mollusk and inducer technology, which is used in medical raw materials from birds, drug combinations, sexual diseases, etc., can solve the problems of long stimulation time, unstable spawning, and low spawning efficiency, and achieve induction efficiency. High, improve spawning efficiency, simple operation effect

Active Publication Date: 2018-06-05
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods to stimulate the spawning of marine bivalve molluscs mainly include physical methods such as drying in the shade, flowing water and variable temperature stimulation, chemical methods such as ammonia water and ultraviolet radiation stimulation, and biological methods such as semen stimulation, but the spawning is generally unstable and the spawning efficiency is low. , long stimulation time and other disadvantages

Method used

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  • Inductive agent for inducing marine bivalve mollusk to produce eggs as well as application method thereof
  • Inductive agent for inducing marine bivalve mollusk to produce eggs as well as application method thereof
  • Inductive agent for inducing marine bivalve mollusk to produce eggs as well as application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Under the conditions of water temperature: 23~25℃, salinity: 28~30, pH value: 7.6~7.9, set up six groups of hard-shell clams, three of which use 1.07 g / m 3 , 1.50 g / m 3 and 1.37 g / m 3 The concentration of boiled egg yolk inducer was splashed, and the other three control groups were subjected to dry stimulation for 8 hours, water stimulation for 6 hours, and ultraviolet radiation for 4 hours, respectively, while other conditions remained unchanged. At the end of the experiment, the spawning rate of the brood clams in each group was counted, and the results are shown in Table 1:

[0017] Table 1: Induction effect of boiled egg yolk on broodstock oviposition of hard-shelled clams

[0018]

[0019] It can be seen from Table 1 that when boiled egg yolk with different splashing concentrations was used as inducer, the spawning rate of hard clam broodstock was 36%, 53% and 42% higher than other induction methods, and the laying rate of hard clam broodstock The egg rate ...

Embodiment 2

[0021] Under the conditions of water temperature: 28~30℃, salinity: 25~28, pH value: 7.5~8.2, set up six groups of clam parenting experiments, three of which use 1.32 g / m 3 , 1.43 g / m 3 and 1.07 g / m 3 The concentration of boiled egg yolk inducer was splashed, and the other three control groups were subjected to dry stimulation for 7 hours, water stimulation for 5 hours, and ultraviolet radiation for 4 hours, respectively, while other conditions remained unchanged. At the end of the experiment, the spawning rate of clams in each group was counted, and the results are shown in Table 2:

[0022] Table 2: Induction effect of cooked egg yolk on oviposition of clam broodstock

[0023]

[0024] It can be seen from Table 2 that when different concentrations of boiled egg yolk were used as inducers, the oviposition rate of clam broodstock was 31%, 44% and 35% higher than other induction methods, respectively, and the concentration of boiled egg yolk inducer was 1.43 g / m 3 Th...

Embodiment 3

[0026] Under the conditions of water temperature: 23~24℃, salinity: 28~30, pH value: 7.5~8.0, set up six groups of scallop parenting experiments, three of which use 1.12g / m 3 , 1.34 g / m 3 and 1.27 g / m 3 The concentration of boiled egg yolk inducer was splashed, and the remaining three control groups were stimulated by drying in the shade for 4 hours, running water for 5 hours, and warming for 3°C, respectively, while keeping other conditions unchanged. At the end of the experiment, the spawning rate of the broodstock of Chlamys farreri in each group was counted, and the results are shown in Table 3:

[0027] Table 3: Induction effect of boiled egg yolk on oviposition of Chlamys farreri broodstock

[0028]

[0029] It can be seen from Table 3 that when different concentrations of boiled egg yolk were used as inducers, the egg laying rate of Chlamys farreri broodstock was 38%, 30% and 36% higher than other induction methods, respectively. The spawning rates of broodstock...

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Abstract

The invention relates to an aquaculture method of shells, in particular to an inductive agent for inducing marine bivalve mollusk to produce eggs as well as an application method thereof. The application method comprises the following steps: taking cooked egg yolk liquid obtained through bolting-silk treatment as the inductive agent, splashing the inductive agent in a parent shellfish pool uniformly according to the concentration, being 1.0 to 1.5 g / m<3>, of the cooked egg yolk under the conditions that the water temperature is 20 to 30 DEG C, the salinity is 25 to 35 and the pH value is 7.5 to 8.3, treating for 0.5 to 1 hour, performing pool separation according to a siphonage method after the parent shellfishes lay eggs, fully filling the pool with water again, and diluting the concentration of cooked egg yolk. Compared with other stimulation methods, the method has the following advantages: the egg producing efficiency can be increased by 30 to 50 percent and the egg producing efficiency of the parent shellfishes can be effectively improved. The inductive agent can be applied to various marine bivalve mollusk such as hard-shelled clam, clam, short-necked clam, cyclina sinensis gmelin, bay scallop, chlamys farreri and patinopecten yessoensis.

Description

technical field [0001] The invention relates to a shellfish aquaculture method, in particular to an inducer for inducing marine bivalve shellfish to lay eggs and application thereof. Background technique [0002] Bivalve mollusk is an important economic species of mariculture in my country, and improving the spawning efficiency of broodstock is of great practical significance for improving the efficiency of seedling raising. The successful discharge of sperm and eggs by marine bivalve molluscs for fertilization is the prerequisite for successful shellfish breeding. Whether bivalve mollusc brood can successfully spawn is not only related to the degree of gonad development of the brood brood, but also affected by environmental stimuli. At present, the methods to stimulate the spawning of marine bivalve molluscs mainly include physical methods such as drying in the shade, flowing water and variable temperature stimulation, chemical methods such as ammonia water and ultraviolet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/57A61P15/08
CPCA61K35/57Y02A40/81
Inventor 张涛徐涛于金诚胡志于正林宋浩杨美洁
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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