Preparation method of patinopecten yessoensis gonad zymolyte/kappa-carrageenan mixed gel
A technology for mixing scallops and scallops with gelatin, which is applied in food science, fermentation and other directions, can solve problems such as single nutrition, and achieve the effect of simple operation process, good effect, and improving gel properties.
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Embodiment 1
[0040] S1. Raw material pretreatment: take fresh male scallop gonads, rinse them with deionized water, heat at 95°C for 10 minutes, cool to room temperature, vacuum freeze-dry at -25°C for 72 hours, grind and pulverize gonads of scallops lyophilized powder ;
[0041]S2, enzymatic hydrolysis reaction: the protein in the scallop gonad freeze-dried powder prepared in step S1 was measured by the Kjeldahl method, accounting for 83% of the dry basis of the freeze-dried powder, and the scallop gonad freeze-dried prepared in step S1 was weighed Powder 2.4g (the 2.4g lyophilized powder contains 2g of protein), add 50ml of deionized water and mix well, the protein concentration in the solution is 40g / L, adjust the pH of the solution to 8.0 with 0.5mol / L NaOH, add to the solution 6000U trypsin (the amount of trypsin added is 3000U / g protein), enzymatically hydrolyzed at 35°C for 3h, heated in a boiling water bath for 10min, and cooled to obtain the enzymatic hydrolyzate of gonad of Ezo s...
Embodiment 2
[0048] S1. Raw material pretreatment: take fresh male scallop gonads, rinse them with deionized water, heat at 80°C for 10 minutes, cool to room temperature, vacuum freeze-dry at -50°C for 60 hours, grind and pulverize gonads of scallops lyophilized powder ;
[0049] S2, enzymatic hydrolysis reaction: the protein in the scallop gonad freeze-dried powder prepared in step S1 was measured by the Kjeldahl method, accounting for 83% of the dry basis of the freeze-dried powder, and the scallop gonad freeze-dried prepared in step S1 was weighed Powder 2.4g (the 2.4g lyophilized powder contains 2g of protein), add 50ml of deionized water and mix well, the protein concentration in the solution is 40g / L, adjust the pH of the solution to 8.0 with 0.5mol / L NaOH, add to the solution 6000U trypsin (the amount of trypsin added is 3000U / g protein), enzymatically hydrolyzed at 40°C for 3h, heated in a boiling water bath for 10min, and cooled to obtain the enzymatic hydrolyzate of gonad of scal...
Embodiment 3
[0056] S1. Raw material pretreatment: take fresh male scallop gonads, rinse them with deionized water, heat at 90°C for 10 minutes, cool to room temperature, vacuum freeze-dry at -35°C for 65 hours, grind and pulverize gonads of scallops lyophilized powder ;
[0057] S2, enzymatic hydrolysis reaction: the protein in the scallop gonad freeze-dried powder prepared in step S1 was measured by the Kjeldahl method, accounting for 83% of the dry basis of the freeze-dried powder, and the scallop gonad freeze-dried prepared in step S1 was weighed Powder 2.4g (the 2.4g lyophilized powder contains 2g of protein), add 50ml of deionized water and mix well, the protein concentration in the solution is 40g / L, adjust the pH of the solution to 8.0 with 0.5mol / L NaOH, add to the solution 6000U trypsin (the amount of trypsin added is 3000U / g protein), enzymatically hydrolyzed at 38°C for 3h, heated in a boiling water bath for 10min, and cooled to obtain the enzymatic hydrolyzate of gonad of Ezo ...
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