Method of preparing yeast rich in copper

A technology for the preparation of copper-rich yeast and strains, which is applied in the field of feed additives, can solve the problems of low biological potency, complex production process, and large addition amount of inorganic copper salts, so as to reduce the residue of livestock and poultry manure, improve economic benefits, and promote The effects of digestion and absorption

Inactive Publication Date: 2009-08-19
ZHEJIANG UNIV
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Problems solved by technology

[0003] At present, in China, inorganic copper salts such as high-dose copper sulfate are mostly used as copper nutritional additives, but inorganic copper salts have low biological potency and large additions, which easily cause serious environmental pollution; while they have good stability and high biological potency Amino acid complex copper salts (copper methionine, copper lysine, etc.) limit their application due to their complex production process and high price

Method used

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Examples

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Effect test

preparation example Construction

[0021] The preparation method of the copper-rich yeast firstly prepares the high-copper-tolerant yeast strain, and then uses the high-copper-tolerant yeast strain to prepare the copper-rich yeast.

[0022] 1. The specific examples of preparing high-copper-tolerant yeast strains are as follows:

Embodiment 1-1

[0024] Step a. Select yeast strains with different biochemical characteristics, and inoculate each yeast strain to 10~200μg / ml gradient copper concentration (copper concentration respectively 10μg / ml, 50μg / ml, 150μg / ml, 200μg / ml) Cultivate in the medium for 20 hours, and use the yeast strain with the highest survival rate as the strain to be mutagenized;

[0025] Step b. Inoculate the strain to be mutagenized on a medium containing diethyl sulfate for mutagenesis to form a mutagenized strain;

[0026] Step c. Separate and screen the mutagenized strains and inoculate them in a medium with a gradient of copper concentration of 10~200μg / ml (copper concentrations are 10μg / ml, 50μg / ml, 150μg / ml, 200μg / ml) and culture for 36h, The cultured bacteria liquid is collected, filtered, dried, weighed and tested for its copper content. Mutagenic strains with both biomass and copper content above the average are used as high-copper-tolerant yeast strains.

[0027] The culture conditions of the m...

Embodiment 1-2

[0029] Step a. Select yeast strains with different biochemical characteristics, and inoculate each yeast strain to a gradient of copper concentration of 10~200μg / ml (the copper concentration is 10μg / ml, 40μg / ml, 80μg / ml, 120μg / ml, 160μg respectively. / ml, 200μg / ml) culture medium for 36h, and the yeast strain with the largest survival rate as the strain to be mutagenized;

[0030] Step b. Inoculate the strain to be mutagenized on a medium containing diethyl sulfate for mutagenesis to form a mutagenized strain;

[0031] Step c. Separate and screen the mutagenized strains and inoculate them to a gradient of copper concentration of 10~200μg / ml (copper concentrations are 10μg / ml, 40μg / ml, 80μg / ml, 120μg / ml, 160μg / ml, 200μg / ml ) Culture medium for 36 hours, collect, filter, and dry the cultured bacteria liquid, weigh and detect the copper content, and use the mutagenic strains whose biomass and copper content are both higher than the average value as high copper tolerance Yeast species...

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Abstract

The invention relates to a preparation method of copper-rich yeast. In the invention, high-copper resistant yeast strains are firstly prepared: yeast strains with different biochemical characteristics are cultivated in a culture medium in a gradient copper concentration, and the ones having maximal survival ratio are used as strains to be mutagenized; the strains to be mutagenized are inoculated on the culture medium of diethyl sulfate for mutagenesis; the strains after mutagenesis are inoculated in the culture medium in a gradient copper concentration for cultivation, fungus liquid collection, filtering and drying are carried out, and the strains after mutagenesis with biomass and copper content simultaneously higher than average values are used as the high-copper resistant yeast strains. And then the high-copper resistant yeast strains are used for preparing the copper-rich yeast, and the working procedures are as follows: slant strain cultivation; first-stage liquid seed cultivation; second-stage seed cultivation; fermentation cylinder cultivation; separation drying; grinding; and products of copper-rich yeast. The additives of the copper-rich yeast prepared in the invention greatly improves the absorption utility ratio of copper in animal organisms, increases the economic benefit, and can be applied to copper addition for beasts and birds, and aquatic livestock as feed additives.

Description

Technical field [0001] The invention belongs to the technical field of feed additives, and specifically relates to a method for preparing copper-rich yeast for animal feed. Background technique [0002] Copper is one of the essential trace elements for humans and animals. Copper is an important component of ceruloplasmin and participates in the body's hematopoietic function; copper is also an important component of the copper enzyme lysyl oxidase, which participates in the formation of blood vessel tissues and bones. Maintain the normal elasticity of blood vessels and bone strength; at the same time, copper has an important effect on maintaining the normal pregnancy process, reproduction rate and hatching rate of eggs; in addition, copper plays an important role in regulating the body’s immunity, and it has an important effect on cellular immunity. , Humoral immunity and anti-infection have varying degrees of effect. [0003] At present, most inorganic copper salts such as high-d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23K1/16C12R1/645A23K10/18
Inventor 冯杰马文强
Owner ZHEJIANG UNIV
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