Non-tube rootage method for camellia oleifem tissue culture seedling

A technology of tissue culture seedlings and external roots, applied in the field of plant tissue culture, can solve the problems of poor transpiration resistance and adaptability, low degree of lignification, and small number of effective seedlings, so as to save production costs, seedling space, and root development Good effect of shortening the seedling cycle
CN104094747AActive Publication Date: 2014-10-15JIANGXI ACAD OF FORESTRY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGXI ACAD OF FORESTRY
Publication Date
2014-10-15

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Abstract

The invention discloses a non-tube rootage method for a camellia oleifem tissue culture seedling. The method comprises the steps of inoculating a micro-branch which has 4-5 leaves, a slight red and brighten top leaf, a green stalk and height being 2-4cm and is obtained by performing subculture proliferation on a camellia oleifem seedling onto a root primordium induction culture medium for culturing till the base part generates a white root primordium, wherein each litre of the root primordium induction culture medium contains NAA of 3.0-5.0mg, and the rest root primordium induction culture medium is 1 / 2HB culture medium; taking the micro-branch with the white root primordium growing on the base part, washing the culture medium of the base part without roots and shoots, dipping with GGR (green plant growth regulator) of 200-600mg / L after filtering, instantly cutting onto a cutting medium, watering enough root fixed water, ensuring sufficient water and fertilizer supply during the growth period, and growing the micro-branch to a rooted seedling, wherein the cutting medium is formed by uniformly mixing 2-6 parts of yellow subsoil and 1-2 parts of plant ash. The method adopts non-tube rootage and breaks through the traditional mode in which the root system is induced in a tissue culture bottle, rootage and domestication are organically combined, direct rootage of the camellia oleifem tissue culture seedling outside a tissue culture bottle is realized, and the method has the advantages of short rootage time, well developed root system, absorption function and high transplantation survival rate.
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Description

Technical field:

[0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for rooting out of a bottle of camellia oleifera tissue culture seedlings. Background technique:

[0002] Camellia oleifem is a unique woody oil tree species in my country. The camellia oil produced by camellia oleifera contains more than 90% unsaturated fatty acids, which is easily absorbed and digested by the human body. It has a good effect on reducing serum cholesterol and preventing hyperlipidemia and cardiovascular diseases ; Tea dry and tea husks after oil extraction are also raw materials for extracting tea saponin, xylitol, producing high-protein feed and advanced activated carbon and other comprehensive utilization products. The whole plant of camellia oleifera is a treasure, with high economic value, wide application, strong adaptability, and great potential for comprehensive development and utilization. It is known as "Oriental olive oil". Wit...

Claims

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