Asparagus parent breeding method

Abstract

The invention relates to asparagus parent breeding method, mainly solving the problems that rooting rate is low, root quality is poor and the survive rate of the regeneration plant is low in asparagus organ tissue culture process. The method includes the process that high quality asparagus stem section is selected, culture is carried out in culture medium solution, and asparagus seedling meeting transplant standard is bred. The culture medium solution is confected by the way that hormones corresponding to various culture stages are added on the basis of the basic culture medium solution, the culture includes induction culture, propagation culture, rooting pre-differentiation culture and rooting culture sequentially. Compared with the existing organ culture, the invention has high propagation rate, rooting rate reaches 100%, root system quality is good, and differentiation rate of strong fleshy tap root is improved to 96%, thus ensuring survival rate after transplantation.

Description

technical field

[0001] The invention belongs to the field of parental propagation of herbaceous plants, in particular to asparagus parental propagation technology. Background technique

[0002] The scientific name of asparagus is Diaobo, which is a perennial herbaceous plant of the genus Asparagus in the family Liliaceae. It is a typical dioecious plant, and its hybrid parents can only be reproduced by asexual reproduction.

[0003] The vegetative propagation methods of asparagus include field natural division method and tissue culture method.

[0004] The natural branching method in the field is to dig out the dormant roots of the parent plants from the field when they have grown for more than 3 years, and divide them into 3 to 5 spore groups according to their natural growth state, and then plant them back in the field to achieve purpose of proliferation. This method has very low reproductive efficiency and is not suitable for large-scale parental reproduction.

[0005] ...

Examples

Embodiment 1

[0023] The asparagus parent breeding method of the present invention is to select high-quality asparagus and cut it into a stem section with a well-developed axillary bud, and then culture it in a culture medium solution after being sterilized and washed beforehand, so as to breed the asparagus that can reach the transplanting standard. The process of asparagus seedlings.

[0024] Components and preparation of basic medium solution:

[0025] The composition of this minimal medium solution is as follows: Each liter of solution contains NH 4 NO 3 1650mg, KNO 3 1900mg, CaCl 2 2H 2 O 440mg, MgSO 4 ·7H 2 O 370mg, KH 2 PO 4 170mg, KI 0.83mg, H 3 BO 3 6.2mg, MnSO 4 4H 2 O 22.3mg, ZnSO 4 ·7H 2 O8.6mg, Na 2 MoO 4 2H 2 O 0.25mg, CuSO 4 ·5H 2 O 0.025mg, CoCl 2 ·6H 2 O0.025mg, Na 2 EDTA (disodium ethylenediaminetetraacetic acid) 37.3mg, FeSO 4 ·7H 2 O 27.8mg, VB1 (vitamin B1) 0.4mg, VB6 (vitamin B6) 0.5mg, glycine 2.0mg, niacin 0.5mg, inositol 100mg, agar 5g, s...

Embodiment 2

[0034] Embodiment two: (the part not stated in this embodiment is all the same as embodiment one)

[0035] The asparagus parental tissue propagation method of the present invention, the asparagus parental propagation method of the present invention, is to select high-quality asparagus and cut into a stem section with a well-developed axillary bud, and then carry out in the culture medium solution after disinfection and washing pretreatment. The process of cultivating and propagating asparagus seedlings that can reach transplanting standards.

[0036] Components and preparation of basic medium solution:

[0037] The components of the basic medium solution are as follows: every liter of the basic medium solution contains: NH 4 NO 3 1400mg, KNO 3 2000mg, CaCl 2 2H 2 O 600mg, MgSO 4 ·7H 2 O250mg, KH 2 PO 4 260mg, KI 1.2mg, H 3 BO 3 5mg, MnSO 4 4H 2 O 15mg, ZnSO 4 ·7H 2 O 10mg, Na 2 MoO 4 2H 2 O 0.4mg, CuSO 4 ·5H 2 O 0.04mg, CoCl 2 ·6H 2 O 0.04mg, Na 2 ED...

technical field

[0001] The invention belongs to the field of parental propagation of herbaceous plants, in particular to asparagus parental propagation technology. Background technique

[0002] The scientific name of asparagus is Diaobo, which is a perennial herbaceous plant of the genus Asparagus in the family Liliaceae. It is a typical dioecious plant, and its hybrid parents can only be reproduced by asexual reproduction.

[0003] The vegetative propagation methods of asparagus include field natural division method and tissue culture method.

[0004] The natural branching method in the field is to dig out the dormant roots of the parent plants from the field when they have grown for more than 3 years, and divide them into 3 to 5 spore groups according to their natural growth state, and then plant them back in the field to achieve purpose of proliferation. This method has very low reproductive efficiency and is not suitable for large-scale parental reproduction.

[0005] ...

Examples

Embodiment 1

[0023] The asparagus parent breeding method of the present invention is to select high-quality asparagus and cut it into a stem section with a well-developed axillary bud, and then culture it in a culture medium solution after being sterilized and washed beforehand, so as to breed the asparagus that can reach the transplanting standard. The process of asparagus seedlings.

[0024] Components and preparation of basic medium solution:

[0025] The composition of this minimal medium solution is as follows: Each liter of solution contains NH 4 NO 3 1650mg, KNO 3 1900mg, CaCl 2 2H 2 O 440mg, MgSO 4 ·7H 2 O 370mg, KH 2 PO 4 170mg, KI 0.83mg, H 3 BO 3 6.2mg, MnSO 4 4H 2 O 22.3mg, ZnSO 4 ·7H 2 O8.6mg, Na 2 MoO 4 2H 2 O 0.25mg, CuSO 4 ·5H 2 O 0.025mg, CoCl 2 ·6H 2 O0.025mg, Na 2 EDTA (disodium ethylenediaminetetraacetic acid) 37.3mg, FeSO 4 ·7H 2 O 27.8mg, VB1 (vitamin B1) 0.4mg, VB6 (vitamin B6) 0.5mg, glycine 2.0mg, niacin 0.5mg, inositol 100mg, agar 5g, s...

Embodiment 2

[0034] Embodiment two: (the part not stated in this embodiment is all the same as embodiment one)

[0035] The asparagus parental tissue propagation method of the present invention, the asparagus parental propagation method of the present invention, is to select high-quality asparagus and cut into a stem section with a well-developed axillary bud, and then carry out in the culture medium solution after disinfection and washing pretreatment. The process of cultivating and propagating asparagus seedlings that can reach transplanting standards.

[0036] Components and preparation of basic medium solution:

[0037] The components of the basic medium solution are as follows: every liter of the basic medium solution contains: NH 4 NO 3 1400mg, KNO 3 2000mg, CaCl 2 2H 2 O 600mg, MgSO 4 ·7H 2 O250mg, KH 2 PO 4 260mg, KI 1.2mg, H 3 BO 3 5mg, MnSO 4 4H 2 O 15mg, ZnSO 4 ·7H 2 O 10mg, Na 2 MoO 4 2H 2 O 0.4mg, CuSO 4 ·5H 2 O 0.04mg, CoCl 2 ·6H 2 O 0.04mg, Na 2 ED...