External rooting culture method of endangered relict tree species yew seed tissue culture seedlings
A technology of exogenous rooting and tissue culture seedlings, which is applied in the field of tissue culture of endangered tree species of Chinese pine, rooting of water pine seedlings in a bottle, and can solve the problem of not being able to tolerate dirty air, the cones being easily damaged by insects, and being damaged by water and other issues to achieve the effect of improving production efficiency, reducing production costs and simplifying production procedures
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[0030] Example 1
[0031] 1. Experimental materials
[0032] The seeds of Chinese pine were provided by the Forestry Bureau of Doumen County, Zhuhai City.
[0033] 2. Experimental method
[0034] (1) Disinfection of explants and initiation of culture
[0035] Take plump yew pine seeds, wash them with tap water, and wash them with 0.1% HgCl 2 Disinfect for 8 minutes, rinse 3 times with sterile water, and inoculate in MS medium. At 30 days, 50% of the seeds germinated successfully.
[0036] (2) Cluster bud induction
[0037] The terminal buds of aseptic seedlings with 1.5cm hypocotyl were intercepted as explants and inoculated into MS medium + ZT0.1mg.L -1 +IBA0.1mg.L -1 + Carrageenan 9g.L -1 + 30g.L sucrose -1Cluster bud induction was carried out in , and after 30 days of culture, aseptic cluster buds of Chinese pine were obtained; the average cluster bud induction rate reached 80%.
[0038] (3) Subculture multiplication and multiplication
[0039] For the induced cl...
Example Embodiment
[0044] Example 2
[0045] 1. Experimental materials
[0046] The seeds of Chinese pine were provided by the Forestry Bureau of Doumen County, Zhuhai City.
[0047] 2. Experimental method
[0048] (1) Disinfection of explants and initiation of culture
[0049] Take plump cork seeds, wash them with tap water, and wash them with 0.1% HgCl 2 Disinfect for 5 minutes, rinse 3 times with sterile water, and inoculate in MS medium. At 30 days, 45% of the seeds germinated successfully.
[0050] (2) Cluster bud induction
[0051] The terminal buds of aseptic seedlings with 1.5cm hypocotyl were intercepted as explants, and inoculated into MS medium + ZT0.1mg.L -1 +IBA0.05mg.L -1 + Carrageenan 9g.L -1 + 30g.L sucrose -1 Cluster bud induction was carried out in . After 30 days of culture, aseptic cluster buds of Chinese pine were obtained; the average cluster bud induction rate was 75%.
[0052] (3) Subculture multiplication and multiplication
[0053] For the induced cluster bud...
Example Embodiment
[0058] Example 3
[0059] 1. Experimental materials
[0060] The seeds of Chinese pine were provided by the Forestry Bureau of Doumen County, Zhuhai City.
[0061] 2. Experimental method
[0062] (1) Disinfection of explants and initiation of culture
[0063] Take plump cork seeds, wash them with tap water, and wash them with 0.1% HgCl 2 Disinfect for 3 minutes, rinse 3 times with sterile water, and inoculate in MS medium. At 30 days, 40% of the seeds germinated successfully.
[0064] (2) Cluster bud induction
[0065] The terminal buds of aseptic seedlings with 1.5cm hypocotyl were intercepted as explants, and inoculated into MS medium + ZT0.1mg.L -1 +IBA0.3mg.L -1 + Carrageenan 9g.L -1 + 30g.L sucrose -1 Cluster bud induction was carried out in , and after 30 days of culture, aseptic cluster buds of Chinese pine were obtained; the average cluster bud induction rate reached 70%.
[0066] (3) Subculture multiplication and multiplication
[0067] For the induced clus...
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