Immune colloidal gold reagent for detecting sulfamethazine and preparation method thereof

A sulfamethazine, colloidal gold technology, applied in biological tests, antibodies, measuring devices, etc., can solve the problems affecting the accuracy of analysis results, high requirements for instrument maintenance, and cumbersome preprocessing procedures, and is easy to popularize and use. , The effect of fast detection and low price

Inactive Publication Date: 2004-11-17
博顿生物检验技术(杭州)有限公司
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The disadvantages of various chromatography, gas/mass spectrometry and capillary electrophoresis methods are: professional equipment is required; sample pretreatment procedures are cumbersome and demanding; the operation process is complicated and takes a long time; professionally trained technical personnel are required. Operators must have rich relevant experience; operators must understand various interference factors that affect chromatographic analysis, and understand the advantages and disadvantages of the pretreatment method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0007] Adhere one end of the PVC backing to the sample pad, the binding pad in turn, the nitrocellulose membrane in the middle, and the other end to the absorbent pad. The binding pad is coated with anti-sulfamethazine specific monoclonal antibody-colloidal gold label. The nitrocellulose membrane was coated with sulfamethazine-BSA conjugate and goat (rabbit) anti-mouse IgG.

[0008] Prepare according to the following steps: (1) prepare sulfamethazine-BSA conjugate: mix sulfamethazine and BSA in a ratio of 1:5~50 (mol / mol), make sulfamethazine and BSA BSA forms stable particles, and sulfamethazine-BSA conjugates are formed by purification; (2) Preparation of anti-sulfamethazine-specific monoclonal antibodies: multiple immunizations with sulfamethazine-BSA conjugates For Balb / c pure-line mice, mouse splenocytes and myeloma cells are fused in vitro to form hybridoma cells, and positive hybridoma cell lines can be obtained by selective medium screening. A large amount of anti-su...

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Abstract

The invention is a kind of immune colloidal gold reagent for measuring the sulfamethazole and the manufacturing method. The reagent includes a sample cushion, a colloidal gold combination cushion, aeotic acid cellulose film, a suction cushion and a PVC back lining, one end of the PVC back lining is adhered with sample cushion, colloidal gold combination cushion in order, the middle part is adhered with aeotic acid cellulose film, the other end is adhered with a suction cushion. The character lies in: the colloidal gold combination cushion is enclosed with a kind of sulfamethazole resisting specificity single clone antibody-colloidal gold label, the aeotic acid cellulose film is enclosed by sulfamethazole-BSA couplings and sheep (rabbit) antiplague IgG. The reagent has a strong specificity, high sensitivity, quick and simple, cheap, and convenient.

Description

technical field [0001] The invention relates to an immune colloidal gold reagent for detecting antibodies, and also relates to a preparation method of the reagent. Background technique [0002] Existing methods for detecting sulfamethazine mainly include high-performance liquid chromatography, gas / mass spectrometry (GC / MS), gas chromatography, thin-layer chromatography, spectrometry and wave spectrometry, capillary electrophoresis and supercritical fluid chromatography. The disadvantages of various chromatography, gas / mass spectrometry and capillary electrophoresis methods are: professional equipment is required; sample pretreatment procedures are cumbersome and demanding; the operation process is complicated and takes a long time; professionally trained technical personnel are required. Operators must have rich relevant experience; operators must understand various interference factors that affect chromatographic analysis, and understand the advantages and disadvantages of...

Claims

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Application Information

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IPC IPC(8): A61K39/395G01N33/532G01N33/558G01N33/68
Inventor 张少恩
Owner 博顿生物检验技术(杭州)有限公司
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