3results about How to "Improve rooting efficiency" patented technology

The invention discloses an optimization method of a pea tissue culture regeneration system. The optimization method comprises the following steps: step 1, preparing pea aseptic seedlings; step 2, preparing an explant; step 3, inducing cluster buds; 4, carrying out bud elongation culture; step 5, rooting culture; and 6, domesticating and transplanting regenerated seedlings. By systematically optimizing the tissue culture conditions of the peas, an efficient regeneration system is established and is suitable for multiple pea varieties, and the problem of low regeneration efficiency in the prior art is solved. By means of the optimization method, researchers can obtain high-efficiency regenerated pea plants in a short time, the success rate and rooting efficiency of tissue culture regeneration are improved, meanwhile, the survival rate of regenerated seedlings is increased, and an important technical guarantee is provided for molecular breeding and gene modification of peas and other leguminous crops.

The application belongs to the technical field of plant cultivation and particularly relates to a Blumea riparia growth medium, a tissue culture medium, a tissue culture method and application. The Blumea riparia growth medium takes MS medium as a basic medium and further comprises 0.2-0.3 mg / L NAA, 0.08-0.16 mg / L DA-6, 10 mg / L vitamin C, 20 mg / L myo-inositol, 30 g / L sucrose and 6 g / L agar. DA-6 and NAA are jointly applied to improve the rooting efficiency, root length and plant height of Blumea riparia. The Blumea riparia germplasm with high content of levorotatory borneol is used as an explant to carry out tissue culture and seedling raising, the Blumea riparia tissue culture seedling has high content of levorotatory borneol, and Blumea riparia stable genetic good seedlings can be produced.

The application discloses a culture method for improving rooting efficiency of Phoebe sheareri tissue culture seedlings, and the rooting efficiency of the Phoebe sheareri tissue culture seedlings is improved through multiple steps of seed disinfection, germination culture, external hormone treatment, active carbon adsorption of secondary metabolites and culture medium optimization, so that the tissue culture efficiency of the Phoebe sheareri is systematically optimized and improved, the survival rate of the Phoebe sheareri tissue culture is improved, and the rooting rate of the Phoebe sheareri is improved from 6.57% to 100%. In addition, the addition of active substances significantly promotes the bolting growth of the Phoebe sheareri, and the bolting rate is increased by about 20% compared with the culture medium without the addition, thereby providing strong support for the subsequent growth of the Phoebe sheareri, especially in the rapid growth of the Phoebe sheareri plant, and providing technical support for commercial application and forestry resource development.