46results about How to "Improve rooting efficiency" patented technology

The invention discloses an ex-vitro rooting method for tissue culture seedlings of pinus massoniana. The ex-vitro rooting method comprises the following steps: taking terminal buds of aseptic seedlings with 0.5cm long hypocotyls as explants for inducing cluster buds in a culture medium I; carrying out elongation culture on the induced cluster buds in a culture medium II, individually cutting off the terminal buds, and inoculating the cut terminal buds into a culture medium III for propagation to obtain subculture buds I; repeatedly elongating the subculture buds I, individually cutting off the subculture buds I, dividing the cut subculture buds I into terminal buds and bud sections, continuously propagating in a subculture propagation culture medium III to obtain subculture buds II, and repeatedly elongating and propagating the subculture buds II to obtain new propagation subculture buds; and transplanting the individually cut 2.5-3.5cm high subculture buds into a seedling culture cup for ex-vitro rooting, and transplanting the ex-vitro subculture buds into a seedling nursery for culture to obtain the ex-vitro tissue culture seedlings. According to the method disclosed by the invention, the production program of test-tube seedlings is simplified, the production cost is reduced, the production efficiency is improved, the rooting treatment time is 25-30 days, the ex-vitro rooting rate reaches 92%-98%, the root system quality is higher, the transplanting survival rate reaches 90%-95%, and the method has better economic, social and ecologic benefits.

The invention relates to a slip pepper isolated organism cultivating technique and method, which uses two kinds of organism cultivate techniques to realize the slip pepper regeneration. The invention is characterized in that it overcomes the technique bottleneck of slip pepper isolated organism cultivation as endotrophic mycorrhiza pollution, with which the slip pepper can be cultivated via various methods, to realize the regeneration and quick cultivation of slip pepper and harmless seed via germ cultivation and callus induction. The slip pepper seed cultivated by the invention is germ free and harmless while th cultivate index in each month can reach 7-8, the root rate can reach 100%, and the transplant survival rate can reach 87.2%. The invention has significant social and economic benefits.

The invention discloses a selenium-rich Hylocereus undulates high efficiency planting method. The planting method includes the following steps: (1) pretreatment of Hylocereus undulates branches: cutting stems off from mother plants of Hylocereus undulates, cutting the stems into stem segments with the length of 15-20 cm, soaking the stem segments into a rooting solution, performing auxiliary treatment on the stem segments on a position, 70-100 cm away from an ultraviolet radiator having a distance wavelength of 320-380 nm and the frequency of 2X10<14-16> Hz, for 30-45 min, and acquiring the treated Hylocereus undulates branches; (2) cuttage and seedling cultivation; and (3) fertilizing management: applying a selenium-rich organic fertilizer once every 3 months during growth of the Hylocereus undulates seedlings, and spraying the Hylocereus undulates seedlings with a selenium-rich solution once every 8 days during a period that the Hylocereus undulates branches have buds to a period that flowers fall. The selenium-rich Hylocereus undulates high efficiency planting method can significantly improve the survival rate of Hylocereus undulates branches, can greatly increase the yield and the selenium content of the Hylocereus undulates fruits, and has high market promotion value.

The invention discloses a grafting method of tree-like Chinese roses. The grafting method comprises the first step of loading a matrix into a seedbed, and afterwards laying gauze and black gauze on the seedbed; the second step of selecting branches of Rosa multiflora Thunb. var. carnea Thory as stock cuttings; the third step of inserting the stock cuttings into the matrix, and making the upper ends of the stock cuttings in contact with the gauze; the fourth step of during cultivation, using the black gauze to shade light within 10-14 days after cuttage of the stock cuttings, maintaining the humidity to be 70-75% and the temperature to be 22-25 DEG C, afterwards removing the black gauze, and maintaining the humidity to be 50-55%, wherein when light treatment is conducted, the temperature is 26-30 DEG C, and when dark treatment is conducted, the temperature is 8-10 DEG C; the fifth step of after cultivation is conducted for 4-5 months, the stock cuttings are cultivated into tree-like Chinese rose stocks, in 20 days before transplanting is conducted, using the tree-like Chinese rose stocks as the circle center, digging first annular ditches around the radius of the stocks with the radius being 50-60 cm, in 10 days before transplanting is conducted, digging second annular ditches around the radius of the stocks with the radius being 30-40 cm, and in 2-3 days before transplanting is conducted, digging third annular ditches around the radius of the stocks with the radius being 10-15 cm, and then conducting transplanting when transplanting is needed; the sixth step of conducting tree-like Chinese rose grafting from the middle ten days to the last ten days of October every year.

The invention belongs to an efficient nursery seedling raising technology in the technical field of agriculture and forestry, and particularly discloses a vine tea seedling raising method that can root efficiently in a laminated manner. The vine tea seedling raising method comprises two key technical links: using first-level nursery lamination processing cuttage twigs to promote rooting; using second-level nursery to grow strong seedlings. The method adopts two-segment type seedling raising, and avoids extensive cuttage management; the method adopts a laminated method for seedling raising, uses low temperature in winter to reduce infection and mold rot, greatly promotes cutting slips to form callus, and improves rooting efficiency; with the two-segment type seedling raising and the laminated technology combined, the method improves the survival rate of cuttage and the rate of strong seedlings.

The invention relates to the field of plant growing, and provides a rapid propagation method for calatheaveitchiana. The method is used for improving the propagation efficiency of the calatheaveitchiana. The rapid propagation method for the calatheaveitchiana comprises the steps that a calatheaveitchiana female parent is taken, a lateral bud is taken, part of a leaf sheath is stripped, and then the lateral bud is cleaned, then soaked with ethyl alcohol and immersed into a disinfection solution for disinfection; part of the leaf sheath is stripped, and the lateral bud is immersed into the disinfection solution again for a time and then taken out to be cleaned; the remaining leaf sheath is stripped to obtain an explant; the explant is subjected to adventitious bud induction culture until anadventitious bud seedling is obtained; the adventitious bud seedling is taken, the stem section is obtained and subjected to adventitious bud multiplication culture, rooting induction culture is conducted until a complete plant tissue culture seedling with main roots is obtained; the tissue culture seedling is cleaned and then transplanted into the substrate, and a lot of calatheaveitchiana seedlings are obtained. In the culture process, the seedling survival rate is high, more calatheaveitchiana plants can be obtained within the same culture time, and the propagation efficiency is greatly improved.

A cutting seedling rooting agent for zelkova serrata is characterized by being prepared from, by weight, 35-50 parts of ABT rooting powder, 1.5-2.5 parts of portulaca oleracea extracting solution, 2-4 parts of compound sodium nitrophenolate, 2-4 parts of mentha root dry powder, 2-4 parts of purple potato vine dry powder, 0.4-0.6 part of chrysanthemum extract, 1-2 parts of benzoic acid, 0.5-1.5 parts of brassin, 1-2 parts of saponin, 4-6 parts of chelated zinc, 0.4-0.6 part of low-sodium salt, 1.5-2.5 part of humic acid, 0.4-0.6 part of glyceryl behenate, 1.5-2.5 parts of rice vinegar, 1.5-2.5 parts of tea saponin, 1.5-2.5 parts of seaweed micro powder, 0.4-0.6 part of neutral protease, 4-6 parts of 25% carbendazim wettable powder and 0.3-0.5 part of L-glutamic acid. The cutting seedling rooting agent can bring forward rooting and improve the rooting efficiency.

The invention discloses a water culture cuttage rapid propagation method for cinnamomum porrectum, and particularly relates to the technical field of seedling culture. The method comprises the following steps of manufacturing a cuttage water tank, selecting and processing cutting slips, performing cuttage and performing cuttage management. The invention also provides application of the water culture cuttage rapid propagation method for the cinnamomum porrectum to rapid propagation of the cinnamomum porrectum. A water culture method is used for performing cuttage on annual semi-lignified tendertips of the cinnamomum porrectum, and the cutting slips can be promoted to take root in advance; the cuttage rooting efficiency of the cinnamomum porrectum can be obviously improved; the cuttage costis reduced; and the management is convenient.

The invention discloses a Peucedanum praeruptorum rooting culture medium. Every 1000mL of the Peucedanum praeruptorum rooting culture medium contains 0.1-0.3mg of alpha-naphthylacetic acid, 0.1-0.3mgof indoleacetic acid, 0.10-0.18mM of water-soluble neodymium-containing compounds, 0.2-0.8mg of rooting powder, 3-5g of agar powder, 28-34g of cane sugar and the balance of a basic culture medium. Through the rooting culture medium disclosed by the invention, the rooting rate of Peucedanum praeruptorum test-tube plantlets can be increased to 90% or above, and besides, the single-plant rooting number can also be increased to 5 or above, so that the transplanting survival rate of the Peucedanum praeruptorum test-tube plantlets can be greatly increased.

The invention discloses a cutting seedling raising rooting agent for zelkova schneideriana. The cutting seedling raising rooting agent for the zelkova schneideriana, which is provided by the invention, is prepared from the following raw materials in parts by mass: 25 to 50 parts of ABT rooting powder, 1.0 to 3.5 parts of herba portulacae extracting solution, 3 to 6 parts of alkyl glycoside, 1 to 4parts of mint root dry powder, 1 to 4 parts of isomaltotriose, 0.5 to 0.8 part of chrysanthemum extract, 1 to 3 parts of benzoic acid, 0.3 to 1.5 parts of brassin, 2 to 4 parts of saponin, 3 to 6 parts of chelated zinc, 0.3 to 0.6 part of low-sodium salt, 1.2 to 2.5 parts of humic acid, 0.2 to 0.6 part of glyceryl behenate, 1.0 to 2.5 parts of white vinegar, 1.0 to 2.5 parts of tea saponin, 1.5 to 2.5 parts of alga micro mist, 0.4 to 0.6 part of neutral proteinase, 3 to 6 parts of 25 percent carbendazim wettable powder and 0.3 to 0.5 part of L-glutamic acid. The cutting seedling raising rooting agent for the zelkova schneideriana, which is provided by the invention, can be used for advancing the rooting time of a cutting seedling of the zelkova schneideriana and improving rooting efficiency, and the rooting agent can be also used for preventing a nutrition deficiency phenomenon from occurring as a nutrient substance needed by a crop is not supplemented in time after the rooting agentis absorbed by the crop, has balanced nutrients and can be used during all growth periods of the crop.

The invention discloses a rapid rooting cutting breeding method for sabina chinensis. The rapid rooting cutting breeding method includes the following steps that 1, cutting is carried out in September mid to October early every year, cutting roots are disinfected, regenerated roots are induced, then drying in the shade is carried out, and the product is covered with wet cloth for refreshing; 2, ridges are prepared in a greenhouse, the ridges are filled with cutting media, soil dwelling pests are killed, then disinfection is carried out, tilling is carried out, soil is completely watered with water, and covering is carried out with films; 3, cuttings prepared in the step 1 are inserted into the cutting media in the step 2, the temperature and the humidity are controlled, watering is carried out in a timing mode, cutting is carried out for 2 months, then watering is carried out one time every week, and 0.1% of monopotassium phosphate is sprayed for supplementing nutrients; 4, from the end of September to beginning of October of second year, the height of cutting seedlings reaches 20 cm at the moment, and the seedlings are transplanted. According to the rapid rooting cutting breeding method for the sabina chinensis, a bottleneck of cutting of the sabina chinensis in spring is removed, reproduction of the sabina chinensis is quickened, the rooting efficiency of the sabina chinensis is improved, the survival rate of reproduction is 98% or above, and reproduction plants of the sabina chinensis are increased by one time or above every year.

The invention discloses a rooting agent for raising a cutting seedling of a zelkova serrata. The rooting agent comprises indole butyric acid and talcum powder, wherein the mass of the indole butyric acid in each kilogram of rooting agent is 1300-1600 mg. The rooting agent for raising the cutting seedling of the zelkova serrata can shorten time for rooting and can improve rooting efficiency, a zelkova serrata scion branch processed by the rooting agent can grow axillary buds after around 22 days of cutting, after three days of the buding of the scion branch, little amount of culluses are generated at a lower cutting opening of the scion branch; along with the rising of the temperature, after about 40 days, all axillary buds grow out, spires are grown, and about 80% of scion branch generates calluses; the rooting of the processed scion branch is mainly based on calluses in an intensive and bursting manner; the quantity of roots is large, branch roots are strong and the number of the branch root can even be hundreds.

The invention discloses a method for evidently increasing Litsea cubeba cluster bud rooting rate. The method includes the steps of firstly, separating Litsea cubeba cluster buds to obtain single buds;secondly using an anti-browning agent perform soaking pretreatment on the base cut of each single bud, wherein the anti-browning agent comprises astaxanthin; thirdly, inoculating the pretreated single buds to a seedling strengthening culture medium to perform seedling strengthening culture; fourthly, transferring the single buds after the seedling strengthening culture to a primary culture mediumto perform primary rooting culture to induce root primordium formation; fifthly, transferring the single buds after the primary rooting culture to a subculture medium to perform rooting subculture toobtain rooted seedlings, wherein the subculture medium comprises Litsea cubeba root tip tissue extract. The method has the advantages that cluster bud death caused by tissue browning during culture can be reduced greatly, the rooting efficiency and root growth speed of the Litsea cubeba cluster buds are increased evidently, Litsea cubeba rapid propagation efficiency is increased, and Litsea cubeba industry development is promoted.

The invention provides a cutting propagation method of clematis armandii. A proper clematis armandii cutting medium is found, a reasonable medium component proportion is set, by matching various cutting propagation details such as corresponding proper cutting depth, node number of cutting slips, leaf number, growth hormone with proper soaking concentration and components and the like, the rooting efficiency and survival rate are improved, the propagation efficiency of the clematis armandii is improved, the propagation yield of the clematis armandii is increased, the production cost is reduced, and the requirements of propagation production and landscape application of clematis armandii resources are met.

The invention provides a special rooting agent for forestry seedlings. The agent comprises, in parts by mass, the following components: 8-12 parts of auxin, 20-50 parts of catechol, 18-26 parts of copper tartrate, 7-26 parts of naphthylacetic acid, 0.1-5 parts of 3-aminopropanol, 20-30 parts of diuron, 50-70 parts of erucyl amide, 1-10 parts of salicylic acid, 0.5-10 parts of benayl aminopurine, 30-60 parts of cytokinin and 50-90 parts of deionized water. The special rooting agent for the forestry seedlings can effectively improve the rooting efficiency, preparation is simple and operation iseasy.

The invention discloses a method for growing seedlings of passion fruit. The seeds of passion fruit are soaked in a potassium permanganate solution with a mass concentration of 1-3%, and then washed with sterile water to obtain sterile seeds; Bacteria seeds are inoculated in the primary culture medium, and the primary culture is carried out to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings are used as explants, and inoculated in the secondary culture medium, and the subculture is carried out to obtain Rootless seedling: described rootless seedling is transplanted in the rooting medium, carries out rooting culture, obtains passion fruit seedling. The method for growing seedlings by tissue culture of the invention has high rooting efficiency and high survival rate, and can maintain good quality of plants.

The invention discloses rooting basic media (BPM) special for prunus maritima marshall tissue culture seedlings and a cultural method thereof. The rooting basic media (BPM) special for the prunus maritima marshall tissue culture seedlings and the cultural method thereof are characterized in that by means of the supporting measures such as improving MS basic medium components, adding rooting inductive agents (NAA and activated carbon) and adjusting culture conditions, the rooting rate in a bottle for 14 d can reach 100%, the transplanting survival rate can reach over 90%, and the transplanting survival rate is increased by 12%-34% than that of a report at present. 45 d is only needed from inoculation to transplanting survival, the efficiency of inducing rooting of the prunus maritima marshall tissue culture seedlings is improved, and cultured seedlings are in order and strong. The method for rapidly and efficiently inducing rooting of the prunus maritima marshall tissue culture seedlings has the advantages that the cost is reduced, the efficiency is high, the culture period of the prunus maritima marshall tissue culture seedlings is shortened, and the method for rapidly producing high quality seedlings is provided for scale production.

The invention discloses a method for improving the rooting efficiency of muskmelon seedlings. The method comprises the steps of soaking muskmelon seeds in a potassium permanganate solution of which the mass concentration is 1 to 3 percent, and then washing the muskmelon seeds by using sterile water to obtain sterile seeds; inoculating the sterile seeds into a primary culture medium for primary culture to obtain sterile seedlings; taking cotyledons and hypocotyledonary axis of the sterile seedlings as explants, inoculating the sterile seedlings into a secondary culture medium for secondary culture to obtain rootless seedlings; transplanting the rootless seedlings into a rooting culture medium for rooting culture to obtain muskmelon seedlings. A tissue culture seedling growth method has the advantages of high rooting efficiency and high survival rate, and good quality of a plant can be maintained.