Kiwi fruit rooting culture and acclimatization and transplanting method

Abstract

The invention provides a kiwi fruit rooting culture and acclimatization and transplanting method. The method comprises the following steps of 1, selecting tissue culture seedlings, wherein the tissueculture seedlings are inoculated into a rooting culture medium, and rooting culture is performed in a sealed mode; 2, performing rooting culture, wherein under the temperature of 25+ / -2 DEG C, the illumination intensity is 1,500-2,000 Lx, the illumination time is 11-13 h, and rooting culture is performed under the condition that light culture is performed for 30-35 d; 3, performing uncovering andacclimatizing, wherein a culture bottle cover is opened, and acclimation is performed for 4-10 d; 4, perfuming silver sand transplanting, wherein the rooting tissue culture seedlings obtained after uncovering and acclimatizing are transplanted into silver sand, irrigation is performed immediately, the sand is watered at a time, when the sand is about to be dry, watering is performed, silver sand acclimatizing is performed in the shady, cool and slightly humid environment under the normal temperature, the silver sand acclimatizing time is 3-4 weeks, and kiwi fruit seedlings are obtained; 5 performing rural soil planting, wherein kiwi fruit seedlings are transplanted into rural soil. According to the method, the Young Golden red No.50 kiwi fruit seedling growing period is shortened, the tissue culture seedling rooting quality is improved, and the transplanting survival rate is high.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a method for rooting culture, seedling hardening and transplanting of kiwifruit. Background technique [0002] Chinese kiwifruit (Actinidia chinensis) is a unique kiwi fruit species in China, with unique taste and high economic value. The planting scale has gradually expanded in recent years. 'Yangshi Jinhong 50' kiwifruit (Actinidia chinensis'YangshiJinhong 50') is an excellent variety selected from Chinese kiwifruit by Jiangsu Yang's Kiwifruit Science Research Institute in 2013. The average fruit weight is more than 100g. Red Heart is not only good-looking, but also delicious, with high sweetness and delicate flesh. It is considered to be a new variety that is expected to surpass 'Hayward'. It has a high evaluation from farmers and has a broad market prospect. Plant tissue culture technology is an asexual reproduction technology, which can effectively guaran...

AI Technical Summary

Problems solved by technology

Although my country's kiwifruit tissue culture technology is becoming more and more mature, kiwifruit is dioecious and the genes are highly heterozygous, and the tissue culture system is affected by the genotype. It is difficult to meet the needs of agricultural production by seed grafting

Rooting and seedling hardening are the key and difficult points of tissue culture technology, and there is no report on the method of rooting and hardening seedlings of 'Yangshi Jinhong 50' kiwifruit

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