Black fungus selenium-rich culture medium and method for producing black fungi thereof
A technology of black fungus and culture medium, applied in the field of black fungus liquid fermentation medium and its use of liquid strain technology to produce black fungus, can solve the problems of low nutritional content of products, long culture period, low efficiency, etc., and achieve the goal of improving selenium The effect of content, easy source and simple process
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[0051] Example 1
[0052] Preparation of liquid selenium-enriched strains: add potato flour 2.5%, bran flour 3.0%, corn flour 3.5%, sugar 0.7%, glucose 0.3%, sodium selenite 5mg / kg, potassium dihydrogen phosphate 100g into the fermentation tank , 50g of magnesium sulfate, 100L of water, canned, sterilized, cooled, and injected into 1000mL of the liquid parent species of black fungus in a bottle under aseptic conditions, the inoculum volume is 2% by volume; the fermentation culture temperature is 23±2°C, ventilation The volume is 105±5m 3 / h, the initial pH value was 7.0, and the fermentation was carried out for 6 days. The established black fungus was used to produce liquid selenium-enriched strains industrially;
[0053] Solid selenium-enriched medium: material preparation: in percent by weight, weigh 8700 g of sawdust, 1000 g of selenium-enriched Juncao, 200 g of wheat bran, and 100 g of gypsum, for subsequent use; and add 350 g of fresh soybean oil, bag, sterilize, and coo...
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[0055] Example 2
[0056] Preparation of liquid selenium-enriched strains: add potato flour 2.5%, bran flour 3.0%, corn flour 3.5%, sugar 0.7%, glucose 0.3%, sodium selenite 5mg / kg, potassium dihydrogen phosphate 100g into the fermentation tank , 50g of magnesium sulfate, 100L of water, canned, sterilized, cooled, and injected into 1000mL of the liquid parent species of black fungus in a bottle under aseptic conditions, the inoculum volume is 2% by volume; the fermentation culture temperature is 23±2°C, ventilation The volume is 105±5m 3 / h, the initial pH value was 7.0, and the fermentation was carried out for 6 days. The established black fungus was used to produce liquid selenium-enriched strains industrially;
[0057] Solid selenium-enriched medium: material preparation: in percent by weight, weigh 8200 g of sawdust, 1500 g of selenium-enriched Juncao, 200 g of wheat bran, and 100 g of gypsum, for subsequent use; and add 350 g of fresh soybean oil, bag, sterilize, and coo...
Example Embodiment
[0059] Example 3
[0060] Preparation of liquid selenium-enriched strains: add potato flour 2.5%, bran flour 3.0%, corn flour 3.5%, sugar 0.7%, glucose 0.3%, sodium selenite 5mg / kg, potassium dihydrogen phosphate 100g into the fermentation tank , 50g of magnesium sulfate, 100L of water, canned, sterilized, cooled, and injected into 1000mL of the liquid parent species of black fungus in a bottle under aseptic conditions, the inoculum volume is 2% by volume; the fermentation culture temperature is 23±2°C, ventilation The volume is 105±5m 3 / h, the initial pH value was 7.0, and the fermentation was carried out for 6 days. The established black fungus was used to produce liquid selenium-enriched strains industrially;
[0061] Solid selenium-enriched medium: material preparation: in percent by weight, weigh 6100 g of sawdust, 2300 g of selenium-enriched Juncao, 1500 g of wheat bran, and 100 g of gypsum, for subsequent use; and add 350 g of fresh soybean oil, bag, sterilize, and co...
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