47results about How to "Inoculation speed" patented technology

The invention belongs to the technical field of biology deep fermentation technology and particularly relates to a fast manufacture method for liquid strain. The method comprises the steps that for a culture medium, rice powder serves as a carbon source, sugar serves as an auxiliary carbon source, corn powder serves as a nitrogen source, peptone and yeast extract (powder) serve as auxiliary nitrogen sources, monopotassium phosphate and magnesium sulfate serve as inorganic salts, and water serves as a carrier; a glass container serves as a culture container; inoculation is performed after a liquid medium is sterilized, and the liquid strain is obtained through a rotary type shaking table. The fast manufacture method for the liquid strain has the advantages that cheap plant starch is used as the main carbon source and the nitrogen sources, and nutrition-allocated proportion is reasonable and comprehensive; the prepared liquid medium is appropriate in viscosity; a culture period is short, and the liquid strain with good quality, strong vitality and different sizes can be cultured in 2-3 days; at the time of inoculation, the fast manufacture method for the liquid strain is simple, convenient and fast; when a solid culture material is inoculated, the material flows fast and is easy to scatter, many strain running points appear and germinate fast and the like.

The invention relates to an inoculation method of an edible fungus, particularly to a highly efficient inoculation method of the cultivated stems of the edible fungus. The method comprises steps of: construction of a solar greenhouse plastic fungus producing shed; preparation of bagged fungus stems; preparation of medium: 82 percent of saw dust, 17 percent of wheat bran and 1 percent of gypsum; mechanical bagging, sterilization under atmospheric pressure and then moving into the fungus producing shed for cooling; inoculation in the fungus producing shed: after routine sterilization, cooled fungus stems are piled in single layer from east to west, 4 pores are pricked on each stem, then inoculation is carried out and thin film is used for covering. After the first layer of the stems are inoculated, piling in single layer is carried out again from east to west and then inoculation is carried out, and repeat the operation; each pile has 10 to 12 layers; the fungus stems after being inoculated are kept immovable at original places for hypha growing, piles are shifted when the hypha seals the pores of strain or connects with each other, and turn into fungus growth management. The inoculation method of the invention has the advantages that: integrates the inoculation place and the hypha growing and fungus producing place, reduces costs of equipment and manpower, needs no externally sleeved bag, thus saving time, labor and cost; allows cooperation of a plurality of people, thus increasing production efficiency by 1 time; has high inoculation speed, thus reducing the settling probability of mixed bacterium and high rate of finished products. Therefore, the method is applicable to scaled cultivation or factory production.

The invention discloses a method for cultivating cistanche tubulosa in a greenhouse, belonging to the technical field of plant cultivation. The method comprises the following steps: cutting tamarix chinensis in the greenhouse in spring in the first year, simultaneously inoculating the cistanche tubulosa, and carrying out reasonable light warm water fertilizer management after inoculating; and digging the cistanche tubulosa medicinal materials in autumn in the next year or harvesting seeds after the tamarix chinensis bears fruits in spring in the third year, wherein, the cultivation condition after inoculating is natural illumination, the indoor temperature is 18-32 DEG C, and the soil water content is 50-70%. The cultivation method carries out intensification management in the greenhouse, and has the advantages of simple and convenient operation, high cistanche tubulosa vaccination rate, rapid inoculation speed, short production period, high yield, and no freeze injury in winter.

The invention discloses a pholiota nameko three-grade seed production method taking amorpha fruticosa braches as base raw materials. The pholiota nameko three-grade seed production method comprises the following steps: obtaining branch wood sections, treating the branch wood sections, distributing wheat bran on the branch wood sections, preparing an auxiliary culture base material, forming a strain material bag, sterilizing at high pressure and inoculating. The auxiliary culture base material is prepared from the following materials in percentage by mass: 72%-85% of saw dust, 15%-25% of wheat bran, 0.5%-2% of gypsum and 0.5%-2% of lime; water is added into the materials and is uniformly mixed, and the moisture content is controlled to be about 65%. By the aid of the method for preparing pholiota nameko three-grade seeds, the fungi growth time can be shortened for 20-30 days, the inoculating speed is rapid and the pollution rate is greatly reduced; mycelia are thick and dense and grow vigorous; strains on the upper part can be prevented from being aged and the strain preservation period is prolonged by more than 30-40 days.

The invention discloses a disinfection and inoculation method of microphyte seeds and pollen. A special disinfection and inoculation technology for small seeds and plant pollen in a tissue culture process is designed by utilizing seeds and plant pollen of microphytes including orchids as materials in combination of a disinfection method of the tissue culture technology and tools such as an injector and the like; according to the technology, the problems that effective disinfection cannot be realized and a disinfectant cannot be completely removed due to too small orchid seeds and most plant pollen are solved, and the contamination rate in the tissue culture can be effectively reduced to be lower than 5%. Besides, with the adoption of the technology, disinfection and inoculation speeds can be greatly increased, meanwhile, due to the fact that a low-concentration sodium hypochlorite solution can effectively destroy seed coats of the microphyte seeds and cell walls of the pollen and cannot damage seed embryos and pollen cells, accordingly, the microphyte seeds of the plants such as the orchids and the like or the pollen cells of the plants can absorb nutrition of a culture medium more easily, and the seeds germinate 3-5 days in advance.

The invention discloses an edible fungus liquid strain storing and inoculating integrated machine. The edible fungus liquid strain storing and inoculating integrated machine is formed by connection of a storing device and an inoculating device. The storing device comprises a liquid strain storage bottle, a bottle cap and an O-shaped seal ring, wherein the O-shaped seal ring is arranged between the bottle neck and the bottle cap of the liquid strain storage bottle. The inoculating device comprises an inoculating syringe needle, a bend and a silicon rubber hose, wherein the inoculating syringe needle is connected with the silicon rubber hose through the bend, a hole is formed in the bottle cap, the bend and the silicon rubber hose penetrate through the hole to be inserted into the liquid strain storage bottle, and the external diameter of the bend is matched with the internal diameter of the hole. The edible fungus liquid strain storing and inoculating integrated machine has the advantages that the structure is simple, storing and inoculating of liquid strains are integrated, operation is simple and convenient, the inoculating speed is high, labor intensity of workers can be relieved obviously, and production efficiency is improved.

The invention discloses a large-scale aseptic-manipulation continuous production equipment and relates to the technical field of structures of large-scale aseptic-manipulation continuous production lines. The production equipment has the structure that a conveyor is arranged on the frame; an inlet platform, an inlet buffering barn, a sterilizing barn, a middle buffering barn, an inoculation barn,an outlet buffering barn and an outlet platform are orderly arranged above the conveyor from left to right; an ozone generator is arranged above the inlet buffering barn and is connected with the sterilizing barn through a pipeline; the inlets and the outlets of the inlet buffering barn, the middle buffering barn and the outlet buffering barn are respectively provided with a movable curtain; a dehumidification window is arranged above the inoculation barn; both sides of the inoculation barn are respectively provided with three pairs of protective sleeves; and an object table is arranged in themiddle of the interior of the inoculation barn. The production equipment is an inoculation machine having the functions of environment sterilization, material conveying, oxygen enhancement, dehumidification and continuous inoculation, greatly increases the inoculation speed and the certified product rate, reduces the labor intensity and has the advantages of no sterilization residue, no damage and low cost.

The invention discloses a branch strain cultivation method of dictyophora rubrovalvata. The cultivation method includes the following steps: (1) selecting raw materials; (2) selecting branches; (3) processing the branches; (4) preparing corn syrup; (5) performing primary boiling; (6) performing secondary boiling; (7) mixing raw materials; (8) performing sterilization; and (9) performing inoculation. Through the utilization of poplar branches as carriers of strains, scattered germination can be realized when the carriers are placed in culture mediums, so that the strains can grow at 360 degrees; the inoculation method is fast in inoculation, germination and feeding, good in uniform stability of germination, consistent in strain age, fast in inoculation speed and suitable for large-scale production, and can effectively shorten germination time and guarantee inoculation vitality; and the branch strains cultivated by the cultivation method can have the stability of solid strains and the inoculation speed of liquid strain.

The invention relates to the technical field of edible mushroom production, in particular to a mushroom double-sided solid inoculation machine. The machine comprises a frame and a chain conveying mechanism mounted on the frame and a main control cabinet, the chain conveying mechanism is provided with a bacteria pack trough for placing a bacteria pack, a rack is fixed to a purification and disinfection mechanism, the rack is further provided with a first punching mechanism, a second punching mechanism, a first inoculation seed pressing mechanism, a second inoculation seed pressing mechanism, apack turning mechanism and a pack discharging mechanism, the first punching mechanism, the first inoculation seed pressing mechanism, the pack turning mechanism, the second punching mechanism, the second inoculation seed pressing mechanism and the pack discharging mechanism are sequentially arranged in the chain conveying mechanism, and the front ends of the first punching mechanism and the secondpunching mechanism are all provided with bacteria pack disinfection mechanisms; the machine has the advantages of simple structure, convenient use, double-sided inoculation and high work efficiency.

The invention discloses a pleurotus edible fungus rapid proliferation nutrient solution, each 1,000 ml of hyphae rapid proliferation nutrient solution comprises 5 g of saccharose, 5 g of trehalose, 1 g of glucose, 0.05 g of chlorogenic acid, 0.05 g of VB2, 0.05 g of VB6 and the balance of water, and the PH is 7.0. Accordingly, the invention further discloses a pretreatment method of corresponding cultivated species. The proliferation nutrient solution added with different sugars, the vitamins and the antibacterial agent is used for pretreatment on cultivated specie mycelium, the fungus vigor is enhanced, the setting time of mycelium is shortened, the anti-bacterial capability of the mycelium is improved, and therefore, and the sundry fungus pollution rate is reduced while an open type inoculation method is ensured to be adopted. At the same time, a stapler breathable type sealing manner is adopted, oxygen supply during out-of fungus bag culture is enhanced, and the mycelial growth cycle is shortened. Rapid culture, high yield and harmlessness of the pleurotus edible fungus can be realized.

The invention relates to microbial culture and in particular relates to a special inoculation frame special for liquid cell culture. The inoculation frame comprises a base plate and a supporting plate of which the bottom is in running fit with the base plate, wherein a baffle plate is arranged at the rear part of the upper surface of the supporting plate, a bottleneck fixing mechanism is arranged at the front part of the upper surface of the supporting plate, and an angle adjusting device is arranged between the supporting plate and the base plate. The inoculation frame provided by the invention can be used for solving the technical problems of low inoculation speed, high operation strength, high contamination probability and the like of the prior art, and has the advantages of being stable in supporting structure, suitable for clamping fixation of triangular flasks with different specifications, high in inoculation speed, high in effect, saving in labor and the like.

The invention relates to a pollution-free inoculating machine and relates to the technical field of inoculating machines. The pollution-free inoculating machine comprises a frame and a body fixed on the same. The pollution-free inoculating machine is characterized in that the left end of the body is provided with a discharge hole, the right end of the body is provided with a feed hole, an air distribution chamber is arranged in the center of the body and is connected with an air inlet, the bottom of the air inlet is provided with an aerosol smoldering hole, gauze filter layers are disposed at the end of the air inlet and the end of the aerosol smoldering hole, activated carbon filter layers are disposed on left and right sides of the air distribution chamber, each activated carbon filter layer is provided with a raw cotton filter layer, and each of two sides of the air distribution chamber and the inner end of the air inlet is provided with a fan. The pollution-free inoculating machine is reasonable in structural design. Airflow is filtered layer by layer by means of the deseeded and not degreased raw cotton and the activated carbon layers, so that pollution-free inoculation is achieved. Inoculating is easy, so that inoculating process is simplified, and inoculating speed and inoculating success rate are increased.

The invention provides a tissue culture method for rapid expanding propagation of tissue culture seedlings for field planting in the production process of virus-free minitubers of potatoes. The tissueculture method comprises the steps: (1) ammonium nitrate in an MS culture medium formula is replaced with ammonium chloride, and calcium chloride is replaced with calcium nitrate; (2) carrageenan isreplaced with ginned cotton; (3) the using amount of each mother liquor and white sugar is decreased by 10-30%; (4) at 100 DEG C, after two or more minutes, direct filling is conducted, and the filling amount of a 240-type culture bottle is 20-25ml; (5) expanding propagation is conducted, specifically, mother seedlings are shorn into sections each with 1-2 leaves, after the completion of shearing,cutting is not needed, and gentle scattering and even shaking are conducted; and tissue culture is conducted, specifically, the illumination intensity of tissue culture is 3300 Lx. According to the method, the expanding propagation speed is increased by 30% or above, the tissue culture time is shortened by 7 days, and the cost is reduced by 35% or above.